Health screening results of Cubans settling in Texas, USA, 2010–2015: A cross-sectional analysis

Background Protecting the health of refugees and other migrant populations in the United States is key to ensuring successful resettlement. Therefore, to identify and address health concerns early, the US Centers for Disease Control and Prevention (CDC) recommends a domestic medical examination (screening for infectious and noninfectious diseases/conditions) shortly after arrival in the US. However, because refugee/migrant populations often have differing health patterns from one another and the US population, the collection and analysis of health information is key to developing population-specific clinical guidelines to guide the care of resettled individuals. Yet little is known regarding the health status of Cubans resettling in the US. Among the tens of thousands of Cuban migrants who have resettled in the US, some applied as refugees in Cuba, some applied for parole (a term used to indicate temporary US admission status for urgent humanitarian reasons or reasons of public benefit under US immigration law) in Cuba, and others applied for parole status after crossing the border. These groups were eligible for US government benefits to help them resettle, including a domestic medical examination. We reviewed health differences found in these examinations of those who were determined to be refugees or parolees in Cuba and those who were given parole status after arrival. Methods and findings We conducted a retrospective cross-sectional analysis of the Texas Department of State Health Services database. Cubans who arrived from 2010 to 2015 and received a domestic medical examination in Texas were included. Those granted refugee/parolee status in Cuba were listed in federal databases for US-bound refugees/parolees; those who were paroled after arrival were not listed. Overall, 2,189 (20%) obtained either refugee or parolee status in Cuba, and 8,709 (80%) received parolee status after arrival. Approximately 62% of those who received parolee status after arrival at the border were male, compared with 49% of those who obtained prior refugee/parolee status in Cuba. Approximately one-half (45%) of those paroled after arrival were 19–34 years old (versus 26% among those who obtained refugee/parolee status in Cuba). Separate models were created for each screening indicator as the outcome, with entry route as the main exposure variable. Crude and adjusted prevalence ratios were estimated using PROC GENMOD procedures in SAS 9.4. Individuals paroled after arrival were less likely to screen positive for parasitic infections (9.6% versus 12.2%; adjusted prevalence ratio: 0.79, 0.71–0.88) and elevated blood lead levels (children ≤16 years old, 5.2% versus 12.3%; adjusted prevalence ratio: 0.42, 0.28–0.63). Limitations include potential disease misclassification, missing clinical information, and cross-sectional nature. Conclusions Within-country variations in health status are often not examined in refugee populations, yet they are critical to understand granular health trends. Results suggests that the health profiles of Cuban Americans in Texas differed by entry route. This information could assist in developing targeted screenings and health interventions.

a1111111111 a1111111111 a1111111111 a1111111111 a1111111111 who received parolee status after arrival at the border were male, compared with 49% of those who obtained prior refugee/parolee status in Cuba. Approximately one-half (45%) of those paroled after arrival were 19-34 years old (versus 26% among those who obtained refugee/parolee status in Cuba). Separate models were created for each screening indicator as the outcome, with entry route as the main exposure variable. Crude and adjusted prevalence ratios were estimated using PROC GENMOD procedures in SAS 9.4. Individuals paroled after arrival were less likely to screen positive for parasitic infections (9.6% versus 12.2%; adjusted prevalence ratio: 0.79, 0.71-0.88) and elevated blood lead levels (children �16 years old, 5.2% versus 12.3%; adjusted prevalence ratio: 0.42, 0.28-0.63). Limitations include potential disease misclassification, missing clinical information, and cross-sectional nature.

Conclusions
Within-country variations in health status are often not examined in refugee populations, yet they are critical to understand granular health trends. Results suggests that the health profiles of Cuban Americans in Texas differed by entry route. This information could assist in developing targeted screenings and health interventions.

Author summary
Why was this study done?
• Protecting the health of the tens of thousands of refugees and other migrants, including Cubans, resettling in the US is key to ensuring successful resettlement.
• Between 2010 and 2015, Cubans comprised a large portion of migrants resettling in the US arriving by one of two routes: (1) some received either refugee or parole status to enter the US while still in Cuba, and (2) some applied for parole status after arrival in the US.
• Yet little is known regarding the health status of Cubans entering the US (and, more specifically, whether the health status differed by these two routes of entry), and therefore more information is needed to inform screening procedures and health interventions for this population.
What did the researchers do and find?
• We developed mathematical models using data collected from clinic visits shortly after arrival to the US that describe differences in health patterns between these two routes of entry among Cubans who arrived in the US from 2010 to 2015.
• Overall, the health patterns between the two routes of entry may differ with respect to certain health outcomes; specifically, those with parole status after arrival were less likely to screen positive for parasitic infections.

Introduction
Protecting the health of refugee and other migrant populations arriving to the US is key to ensuring successful resettlement. Therefore, because these populations often have differing health patterns than those living in the US, the collection and analysis of health information is key to developing targeted clinical guidelines and interventions to assist US clinicians and public health professionals. However, little is known regarding the health status of Cubans resettling in the US. Yet in 2014, Cubans were among the top five largest groups to resettle in the US, with over 24,000 individuals resettling primarily in Florida, Texas, New York, and California [1]. From 2010 to 2015, the US witnessed an increase in the number of Cubans entering the country, and in 2015 alone, Cuban entries totaled over 43,000 individuals [2]. Historically, Cubans have entered the country as immigrants, refugees, or parolees (individuals allowed temporary admission for urgent humanitarian reasons or reasons of public benefit under US immigration law) for economic, political, and/or health reasons. A refugee is any person who is outside his or her country of nationality or habitual residence and is unable or unwilling to return to or seek protection of that country as a result of a well-founded fear of persecution based on race, religion, nationality, membership in a particular social group, or political opinion [1]. Regarding parole, Cubans could be granted such status while either still in Cuba (where a limited number of parole requests are granted through programs established by the US government, including visa lotteries and family reunification) or at the US border [2][3][4][5]. For the latter, thousands of Cubans were paroled into the US after crossing the border by boat or foot, usually via Florida or the US-Mexico border in Texas from the 1960s to 2017 [2][3][4]. By 2007, the predominant route for these individuals comprised travel by boat or plane to South or Central America and then over land to the US-Mexico border [6]. This route became more common given the difficulties of crossing by boat because those intercepted by the US Coast Guard prior to reaching land were returned to Cuba, as outlined in an agreement between the two countries [6]. Those who reached US soil were permitted to stay under the Cuban Adjustment Act [6]. Those granted parole status in Cuba entered through programs established by the US government, such as family reunification (2007 to present), which notified the US ahead of their arrival [2][3]. According to the Cuban Haitian Entrant Program  policies, all parolees regardless of entry route were eligible to apply for refugee benefits/services, including Refugee Medical Assistance (8 months of health insurance), administered by the Office of Refugee Resettlement [5]. This analysis focuses on Cuban refugees/parolees from both entry routes who received these refugee benefits/services. Before they enter the US, immigrants, refugees, and those given parole status in Cuba undergo an overseas medical examination following the Centers for Disease Control and Prevention's (CDC) Technical Instructions [7]. The purpose of this exam is to identify inadmissible conditions (e.g., tuberculosis disease, syphilis). If an inadmissible condition is identified during this exam, an individual would be required to undergo treatment prior to US entry. Data from these examinations are housed within CDC's Electronic Disease Notification (EDN) system, which includes records for all refugees and Cubans who received parole status in Cuba, and are provided to states where these individuals resettle [8]. The US Department of State's Worldwide Refugee Admissions Processing System (WRAPS), which notifies states of refugee arrivals, also includes records of Cubans granted parole status before leaving Cuba [9]. Cubans paroled at the border (i.e., upon arrival) did not receive an overseas examination and are not included in EDN records.
Regardless of entry route, Cuban refugees and parolees are recommended to undergo a voluntary domestic medical examination within 90 days of arrival in the US [5,10]. This assessment includes a comprehensive physical examination, screening for certain infectious diseases and noninfectious conditions, and vaccination updates [10]. If a positive screening is recorded, the individual is referred to appropriate care. Although the basis of the recommendations for this exam are similar across refugee populations, risk factors for certain diseases vary based on prior country of residence, age, and living circumstances. Much is known about disease prevalence among other populations resettling in the US, but the health status of Cubans has not been previously examined. Additionally, little is known regarding the differences in health status upon arrival between individuals paroled at the border and individuals who obtained refugee/parolee status in Cuba. We hypothesized that, because these two groups differed by entry route, and likely life experiences, we might also observe differences in health status. We compared health records from initial postarrival domestic medical examinations of Cubans entering Texas to identify health differences that can inform targeted screening and long-term health management strategies.

Participants and data collection
We conducted a retrospective cross-sectional analysis of the Texas Department of State Health Services (DSHS) database, examining differences in health status at the voluntary initial domestic (after US arrival) medical examinations among Cubans paroled at the border and those who obtained refugee/parolee status in Cuba. Cuban refugees and parolees who arrived in Texas between January 1, 2010, and September 30, 2015, and who received a domestic medical examination were included.
The Refugee Health Program within DSHS collected health screening and demographic data from all seven of the public health departments that perform domestic medical examinations for refugees and parolees. Data were retrieved from the state's database while the program was under DSHS (in 2016 the program moved to the US Committee for Refugees and Immigrants). DSHS data were cross-referenced with EDN and WRAPS to determine entry route [8][9]. Individuals who obtained refugee/parolee status in Cuba were identified through the presence of a record in EDN and/or WRAPS, indicating they received a predeparture overseas examination and an approval for refugee/parolee status before entry. Those who were paroled at the border were defined as individuals not listed in EDN or WRAPS. Ultimately, data analyzed only included information from the voluntary domestic medical examination for both groups. Data from the overseas examination were not included in the analysis; EDN/WRAPS data were only used to determine entry route.
Of note, all Cuban refugees/parolees listed in EDN as resettling in Texas were found in our data set (i.e., completed domestic medical examination), indicating there were no missing individuals from our denominator among those who obtained status in Cuba. Records were unavailable to determine the true denominator of those paroled at the border, but we assume not all received a domestic medical examination because it is voluntary, and therefore, there are likely some individuals not included from this group. This analysis was not guided by a specific prospective analysis plan. This study is reported as per the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) guideline (S1 STROBE Checklist).

Demographics and health measures
Demographic data and health variables collected during the domestic medical examination included sex; age; body mass index (BMI, calculated using weight and height); blood pressure; hemoglobin and hematocrit results (to identify potential for anemia); blood lead level (BLL); laboratory screening results for hepatitis B (serologic testing of hepatitis B surface antigen, hepatitis B surface antibody, and total hepatitis B core antibody), hepatitis C (antibody test), HIV infection, and eosinophilia; and screening for Mycobacterium tuberculosis infection by tuberculin skin test (TST) and/or interferon-gamma release assay (IGRA). For syphilis, all clinics screened using rapid plasma reagin (RPR) followed by Treponema pallidum particle agglutination (TPPA) assay (three clinics also initially screened with IgG). Two clinics reported only RPR results to DSHS; all others reported results following the full sequence. Clinical results were unavailable to distinguish latent from active infection. Entrants were also screened for parasitic infections (parasites assessed at the state laboratory included Ascaris, Clonorchis, Dientamoeba, amoebas, Giardia, hookworm, and Trichuris by ova and parasite examination using two stool samples per individual; Strongyloides and Schistosoma by serology with an enzyme immunoassay). For all health variables, only screening results are reported. Verified clinical diagnosis data were unavailable. Table 1 provides classifications and interpretations for each health assessment component (additional information on methods for outcome measurement or cutoff values aside from that presented in the text and Table 1 was unavailable).
Because only one blood pressure reading was available (clinical guidance suggests �2 separate readings to diagnose), a hypertension diagnosis was not recorded; rather, individuals were classified as having "normal" or "elevated" blood pressure. Similarly, only "potential for anemia" was recorded because additional factors (pregnancy, menstruation, repeat testing) often accounted for in official diagnoses were unavailable [12]. BMI and blood pressure were analyzed for individuals �18 years old, and BLL for children �16 years, in accordance with CDC guidelines [10,[13][14]. Of those screened for syphilis, 98% were �15 years old, as recommended by CDC [10].  Also in this category would be any persons with a positive HBsAg result who received the hepatitis B vaccination within 18 days before the blood draw for serologies or on the same day as but before the blood draw; however, vaccination and blood draw dates were not available to calculate this interval.

Analysis
Chi-squared comparisons were conducted to examine differences between the two entry routes (α < 0.05). Separate models were created for each component of the health assessment, with entry route as the main exposure variable. Crude and adjusted prevalence ratios (adjPRs), controlling for the county of medical examination (proxy for clinic) to limit bias due to possible differences in screening processes, were estimated using PROC GENMOD procedures (modified Poisson regression approach that ensures robust and conservative error estimation [16]; log-link function) in SAS 9.4 (Cary, NC, USA). Confounding by sex and age and twoway interactions by age (categorical variable; age as a continuous variable did not meet the linear assumption required for this type of model) with entry route and sex with entry route for each model were assessed to determine the most parsimonious model. Interaction terms were retained if significant (P < 0.05), and confounding terms were eliminated if the estimate did not differ by more than 10% without the inclusion of the potential confounder. Polytomous outcomes were dichotomized for interpretability of model results. For BMI, the <2% of individuals categorized as underweight were excluded, and those with normal BMI were compared with those classified as overweight or having obesity combined. Hepatitis B was not modeled because serology results become difficult to interpret in the absence of vaccination history. Hemoglobin/hematocrit and TST/IGRA screening results classified as "indeterminate" were excluded.

Ethical approval
This assessment was approved as nonresearch by the CDC and Texas DSHS. The Refugee Health Program of the Texas DSHS approved the use of these data for analysis. Written consent was not required. women, or >136 kg for men and women) values were excluded because of possible data inaccuracies [11]. 2 CDC guidelines followed [10,[12][13][14]; BMI and blood pressure restricted to �18 years old, and BLL restricted to children �16 years old. 3 Assuming female is not pregnant [12]. 4 Mitruka and colleagues (2018) [15]. 5 Without complete vaccination history, limitations exist to the hepatitis B algorithm used for categorization (HBsAg−/anti-HBc+/anti-HBs− could also indicate lowlevel infection; HBsAg−/anti-HBc−/anti-HBs− could indicate immune from vaccination with anti-HBs waning). 6 If both IGRA and TST were completed and recorded, the IGRA result was used in this analysis. 7 Serologic/laboratory results were unavailable within the data set; all values were reported as positive or negative in the data set. 8 All seven clinics screened using RPR followed by TPPA assay; three clinics also initially screened with IgG prior to this sequence; two clinics reported only RPR results (all others reported after full sequence completed). 9 Antibody test. 10 Parasites assessed: Ascaris, Clonorchis, Dientamoeba, amoebas, Giardia, hookworm, and Trichuris by ova and parasite examination using a stool sample; Strongyloides and Schistosoma by serology.

Results
A total of 10,898 Cubans were included. Of those, 8,709 (80%) were paroled at the border. The remaining 2,189 (20%) obtained refugee/parolee status in Cuba. Of those included, 41% were female, and the average age was 33.5 years (SD: 14.1; 13% �18 years old) ( Table 2). Approximately 66% were overweight or had obesity (33% normal BMI, 1% underweight), and over one-half (56%) had elevated blood pressure. Four percent had a positive TST and/or IGRA, indicating further testing was required to determine tuberculosis disease (�18 years old: 1.2%). Less than 1% of those tested screened positive for hepatitis B, and over one-half (57%) were classified as susceptible (as defined in Table 1). Ten percent screened positive for at least one parasite. In total, 130 individuals screened positive for syphilis (1.2%; all �18 years old), of which 92% were identified in clinics that submitted results after the full RPR/TPPA sequence. Approximately 4% had both abnormal hematocrit and hemoglobin results, suggesting potential anemia (16% abnormal hemoglobin only, 4% abnormal hematocrit only). Of the 1,178 children with valid BLL results, 8% recorded a level higher than CDC's reference level of �5 ug/dL (0.8% �10 ug/dL).
Of those included in the analytic cohort, the majority of individuals paroled at the border entered after 2012 (solid line , Fig 1), and the number of individuals who obtained refugee/ parolee status in Cuba remained relatively unchanged between years (Fig 1). Approximately 62% of those paroled at the border were male, compared with 49% who obtained status in Cuba. Approximately half (45%) of those paroled at the border were 19-34 years old (versus 26% among those who received status in Cuba). Chi-squared tests revealed significant differences in gender, age, BMI, hepatitis B infection and susceptibility, HIV infection, parasitic infection(s), presence of eosinophilia, potential anemia, and elevated BLL (EBBL) between the two entry routes ( Table 2). Table 3 presents crude prevalence ratios and adjPRs. Females paroled at the border were more likely than females who received status in Cuba to be overweight or have obesity (adjPR:

Discussion
Overall, our analysis suggests that the health profiles of individuals paroled into the US at the border and those who obtained refugee/parolee status in Cuba during 2010-2015 may differ with respect to certain health outcomes (e.g., BLLs in children). However, for some health outcomes, our results are inconclusive. The mechanisms for the patterns we observed remain unclear but may include differences in exposures both en route and before departure (potentially related to socioeconomic status or education); duration of the journey from Cuba to the US, which potentially impacted presentation or resolution of the health condition; and receipt of overseas medical examination and treatments provided. Additionally, human migration is complex and influenced by a variety of geopolitical and socioeconomic factors. Therefore, it is also possible the differences observed occurred by chance or were driven by self-selection of route. Surprisingly, we observed a higher risk for EBLL among children who obtained refugee/ parolee status in Cuba. Such patterns could be attributed to differences in exposures within Cuba associated with residential locations, family members' occupations, or unknown socioeconomic factors. For instance, prior analyses found high prevalence of EBLL among Cuban refugee/immigrant children associated with car battery exposure and/or contact with adults whose occupations involved car repair in Cuba [17]. Yet any potential association with these factors and entry route remains unclear. Additionally, although Cuba restricted lead paint use in 1934 [18], the allowable level of lead in paint remains high [19], and the use of leaded gasoline was not restricted until after 1998 [20]. Given the potential for lead persistence in the environment [21], individuals of both entry routes were potentially exposed in Cuba. However, presentation with elevated lead levels in the US could depend on time since exposure. For  1 Where percentages do not add to 100%, values were missing in the data set. 2 Chi-squared statistics; indeterminate results were excluded from chi-squared analysis. 3 Centers for Disease Control and Prevention guidelines followed [10,[12][13][14]; BMI and blood pressure restricted to �18 years old, normal blood pressure (<120 mm HG systolic and <80 mm Hg diastolic), elevated blood pressure (�120 mm HG systolic or �80 mm Hg diastolic), blood lead levels restricted to children �16 years. 4 If both IGRA and TST were completed and recorded, the IGRA result was used in this analysis. 5 All seven clinics screened using RPR followed by TPPA assay; three clinics also initially screened with IgG prior to this sequence; two clinics reported only RPR results (n = 11 of these screened positive; all others reported after full sequence completed). 6 Ascaris, 0.1%; Clonorchis, 0%; Dientamoeba, 0.5%; amoebas, 5.3%; Giardia, 2.2%; hookworm, 0.2%; Trichuris, 0.2%; Strongyloides, 2.2%; Schistosoma, 0.02%. 7 9.5% positive for only one, 0.5% positive for two, and 0.1% positive for three. 8 Ten individuals (0.8%) had a blood lead level value of �10 ug/dL.
https://doi.org/10.1371/journal.pmed.1003233.t002  County was included in the crude model to control for potential bias due to unknown differences between clinics in screening procedures. 2 An asterisk ( � ) denotes interaction between the two terms; terms without an asterisk are confounders. 3 Centers for Disease Control and Prevention Guidelines followed [10,[12][13][14]; BMI and blood pressure restricted to �18 years old, blood lead levels restricted to children �16. 4 If both IGRA and TST were completed and recorded, the IGRA result was used in this analysis. 5 "Indeterminate" classifications were excluded from models. 6 All seven clinics screened using RPR followed by TPPA assay; three clinics also initially screened with IgG prior to this sequence; two clinics reported only RPR results (all others reported after full sequence completed) 7 Using HBG and HCT results. 8 Sensitivity analysis that categorizes potential anemia as abnormal HBG or abnormal HCT: 16% within anemia in total population (15% among those paroled at the border, 19% among those who obtained status in Cuba); crude PR: 0.80 (0.75-0.86); adjusted PR: 0.94 (0.87-1.01). instance, little is known regarding average time spent en route for those paroled at the border; however, it is estimated that some resided in South and/or Central America for months or years [6]. Those who received refugee/parolee status in Cuba typically embarked on direct flights, spending minimal time in travel [6]. Therefore, given our understanding of migration patterns from Cuba, time between last lead exposure in Cuba and the US medical examination was likely greater among those paroled at the border, providing a larger window for elevated levels to decline [22]. Additional analyses are needed to understand the impacts of length of journey and exposures within Cuba on BLL among Cubans in the US.
Syphilis screening positivity was slightly higher among those paroled at the border (1.3% versus 0.9% among those who received status in Cuba). Additionally, our results suggest a higher prevalence of infection (latent or active) among males paroled at the border. However, the RPR/TPPA testing sequence serves only as a screening and cannot provide a confirmed diagnosis of current infection or determine whether an individual is infectious due to testing limitations (i.e., tests are not specific to syphilis, can be impacted by conditions such as pregnancy or immune disorders, and cannot distinguish prior infection from active disease) [23]. A definitive diagnosis requires a medical history and physical examination, which our analysis does not include [23]. Therefore, our values may overestimate active infections because of the potential inclusion of those with previous syphilis (resolved or treated) or those currently or previously positive for other treponemal infections. We were unable to obtain medical or treatment history and thus could not calculate the degree of overestimation. Additionally, comparisons to the US population are difficult because our analysis estimated prevalence, whereas syphilis is typically measured using incidence rates of reported primary and secondary syphilis cases (2016: 8.7 per 100,000 [24]), and no comparable US analyses have examined only RPR/ TPPA positivity. Even US-based prevalent syphilis infections estimates (117,000 estimated infected in 2008) are not entirely comparable, given the likely inclusion of previous infections in our estimates [25]. Ultimately, syphilis is treated during the overseas medical examination, possibly contributing to the lower overall prevalence among those who received refugee/ parolee status in Cuba, yet reasons for differences by sex remain unknown. [7,10].
We expected a higher prevalence of parasitic infections among those paroled at the border given their journey through regions that potentially lacked adequate sanitation and have higher parasitic infection rates compared with Cuba [26][27]. However, our analysis indicated that those who obtained refugee/parolee status in Cuba were more likely to screen positive for parasites. On the other hand, those who crossed the border were more likely to have eosinophilia, often considered a nonspecific proxy for parasitic infections. These discrepant results could be due to other undiagnosed parasitic infections, yet additional data are needed to disentangle this association.
In addition to understanding how these two groups compare, it is also important to compare with the US and other refugee populations. Using this information, clinicians can adjust their screening and intervention strategies to reflect the population being served. For example, we found that combined overweight and obesity prevalence was similar between the adult US population and the adult Cuban population (66% Cubans versus 71% US population; comparison limited by differences in age distributions) [28]. BLLs in children from Cuba were higher than US levels [29]. Compared with many other refugee populations, tuberculosis and hepatitis B infection prevalences were lower among Cubans [15,30]. In general, the Cuban refugee/parolee health profile is more similar to the US population than that of many other refugee populations.
Our analysis was subject to several limitations. Only screening results were used, indicating potential for misclassification and/or prevalence overestimation/underestimation in the event of indeterminate or false positive/negative results. Secondly, only 2010-2015 Texas data were used, and therefore, the results may not be representative of Cubans who resettled outside of Texas or in recent years. Entry route misclassification also cannot be ruled out (e.g., individual received an overseas medical examination but chose to cross the border). Although suspected to be small, it is unclear what proportion could have been misclassified in this manner. Only those who received refugee/parolee status in Cuba received an overseas medical exam; however, because this exam only screens for inadmissible conditions, the impact would only occur in the syphilis and tuberculosis models. Nonetheless, our ultimate goal is to understand health differences between the two groups upon arrival to the US to appropriately treat and care for them after arrival. Therefore, although we cannot control for the medical care prior to US arrival, we do not believe this to be of major issue in terms of our overall message. Additionally, the data set is likely missing individuals paroled at the border who did not receive a domestic medical screening. This omission potentially introduced bias if these individuals were demographically or clinically different from those examined; however, data are not available to assess whether differences exist. Lack of access to clinic-level information prevented the use of laboratory-specific cutoff values for abnormal results, and missing information limited our ability to make complex inferences (e.g., information about pregnancy, which impacts hemoglobin/hematocrit results interpretation, and history of Bacille Calmette-Guerin vaccine, which may cause a false positive TST reaction, were unavailable) [31]. Additionally, without vaccination history, hepatitis B serologic testing interpretation is difficult because, for some, hepatitis B surface antibody wanes postvaccination, yet protection persists through immune memory. In other cases, differentiation between susceptibility and low-level hepatitis B infection using only serology results is difficult. Although 9 of the 10 models included >80% of the study population, missing data limited our interpretations, particularly for hepatitis C. Lack of data availability on socioeconomic status or education level also prevented the ability to adjust our models based on these factors. Ultimately, our estimates are merely a cross-sectional view and can neither identify a directional relationship nor account for differences related to travel time or geographic route. Finally, given the large sample size, even minimal differences were significant. Yet differences in lead levels, parasitic infections/eosinophilia, and syphilis have clinical and public health significance and should continue to be investigated.
Although the Cuban Haitian Entrant Program's policies changed in 2017 regarding those paroled at the border [5], thousands of Cubans entered under its premises [2,6,32]. As outlined, among Cubans residing in the US, there exist two distinct subpopulations that differ in not only life experiences and entry routes but also health profiles. Therefore, understanding the health status of these two groups can be used to inform US-based public health recommendations and develop intervention strategies targeted to each subpopulation.

New contribution to literature
Few analyses have been conducted among Cuban entrants, with most focusing on policy changes across time. Only a small number focused on health, as does our analysis. Additionally, within-country variations in health status are not often examined in refugee populations, yet they are critical to understand granular health trends. Our analysis provides a more indepth view of the health profiles among Cubans upon US arrival and suggests that, although they shared a common country of origin, the health profiles of those paroled at the US border and those who obtained refugee/parolee status in Cuba were different.
Supporting information S1 STROBE Checklist. STROBE, strengthening the reporting of observational studies in epidemiology. (DOCX)