New insights into impaired muscle glycogen synthesis

General rights Unless other specific re-use rights are stated the following general rights apply: Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. • Users may download and print one copy of any publication from the public portal for the purpose of private study or research. • You may not further distribute the material or use it for any profit-making activity or commercial gain • You may freely distribute the URL identifying the publication in the public portal


Perspectives
G lucose is the most rapidly accessible substrate in the body. Its storage as glycogen in muscle and liver is of central importance as a first source of energy for muscle contractions and prevention against hypoglycemia. Glycogen synthesis and breakdown are regulated by insulin and catecholamines as well as by glucose-6-phosphate and the amount of glycogen. There are two isoforms of glycogen synthase (GS), one in muscle and one in liver, encoded by different genes (GYS1 and GYS2, respectively) [1]. Insulin stimulates glycogen synthesis by activating protein phosphatase 1 (PP1), which activates GS, inactivates glycogen phosphatase, and inactivates glycogen synthase kinase 3, an inhibitor of GS. PP1 has a glycogen-targeting subunit (PPP1R3A), which facilitates localization of PP1 to glycogen.

Impaired Insulin-Stimulated Glycogen Synthesis Precedes Type 2 Diabetes
It is well established that patients with type 2 diabetes as well as persons at risk of the disease, i.e., first-degree relatives of patients with type 2 diabetes, are insulin resistant and show impaired insulin-stimulated glycogen synthesis [2,3], possibly as a consequence of impaired stimulation of GS by insulin [4]. It has therefore been suggested that impaired glycogen synthesis could be central in the pathogenesis of type 2 diabetes. A common variant in the GYS1 gene has been associated with type 2 diabetes and insulin resistance, as well as increased risk of cardiovascular morbidity and mortality [5,6]. Carriers of the variant allele of this polymorphism are not able to increase their GS protein in response to exercise [7]. Interestingly, there seems to be an interaction between this polymorphism and exercise in the prevention of cardiovascular disease, i.e., risk genotype carriers do not experience the protective effect of exercise on cardiovascular risk [8].

Inborn Errors of Glycogen Synthesis
Although several inborn errors of metabolism affect breakdown of glycogen (leading to glycogen storage diseases), until recently no genetic defects have been known to affect glycogen synthesis in muscle, only in liver [9]. Newborn children with mutations in both alleles of their GYS2 gene present with severe hypoglycemia. In 2002 a digenic form of severe insulin resistance was reported: in a human pedigree, the patients carried both a stop/frameshift mutation in the regulatory subunit of PPP1R3A as well as a mutation in the gene encoding for the transcription factor PPARγ [10]. Now, in a new study published in PLoS Medicine, the same authors demonstrate that 1.4% (one out of 70) of whites in the United Kingdom carry this stop/ frameshift mutation in PPP1R3A, and that carriers of this mutation show decreased muscle glycogen levels as measured by 13 C mass spectroscopy [11]. They also developed a mouse model carrying the human mutation. In muscle tissue from these mice, the mutant protein failed to bind to glycogen, thus decreasing GS activity and glycogen synthesis. However, there was no increased frequency of diabetes in mutation carriers in the mice.
The authors claim that this is the first genetic defect shown to specifically decrease skeletal muscle glycogen synthesis and content. This was certainly true when this work was performed, but very recently a homozygous mutation was described in the GYS1 gene in children with severe cardiomyopathy and exercise intolerance [12]. In accordance with the liver disease caused by mutations in the GYS2 gene, the disease was called muscle glycogen storage disease 0. The number 0 here implies that there is little or no glycogen formed.
The Perspectives section is for experts to discuss the clinical practice or public health implications of a published article that is freely available online. Stephen O'Rahilly and colleagues describe the effect of a mutation in PPP1R3A, present in 1.36% of participants from one UK population, that directly impairs glycogen synthesis and decreases glycogen levels in human skeletal muscle.

More a Problem of Muscle Contractility Than of Glucose Tolerance
What Are the Clinical Implications?
There are several important implications of these two papers [11,12]. First, genetic defects do cause impaired glycogen synthesis in muscle. Second, impaired glycogen synthesis in muscle does not a priori lead to insulin resistance, impaired glucose tolerance, or diabetes, since glucose tolerance was normal in carriers of mutations in both the PPP1R3A and GYS1 genes. By analogy, glucose tolerance was also normal in mice carrying a mutation in the muscle glycogen synthase gene (the MGSKO mouse) [13]. Rather than affecting glucose tolerance, glycogen deficiency in muscle seems to influence exercise and cardiac performance. It seems that heart muscle, which has twice as much glycogen as skeletal muscle, is more vulnerable to disturbances in glycogen metabolism than skeletal muscle, since glycogen breakdown is the fastest source of energy in the heart. It would be important to study if carriers of the PPP1R3A variant show differences in their exercise and cardiac performance.
Given that mutations in PPP1R3A are seen in one out of 70, and in GYS1 in one out of 100 white people, it will be important to screen for these mutations in individuals with signs of exercise intolerance or cardiomyopathy.
Although impaired glycogen synthesis is a hallmark of insulin resistance and precedes type 2 diabetes, it seems unlikely that it is involved in the pathogenesis of the disease. Impaired glycogen synthesis is rather the consequence of insulin resistance in more proximal steps of intracellular glucose metabolism. In contrast, the previous findings of increased risk of cardiovascular morbidity and mortality in carriers of a common variant in the GYS1 gene may point to the central role of glycogen metabolism for heart muscle.
Taken together, these studies have shed new light on the role of disturbed glycogen synthesis in disease pathogenesis. Genetic screening can now be combined with relevant cardiorespiratory exercise tests and muscle biopsies in individuals with unclear symptoms of exercise fatigue and poor cardiac performance. ◼