Control over gene expression is exerted, in multiple stages of spermatogenesis, at the post-transcriptional level by RNA binding proteins (RBPs). We identify here an essential role in mammalian spermatogenesis and male fertility for ‘RNA binding protein 46’ (RBM46). A highly evolutionarily conserved gene, Rbm46 is also essential for fertility in both flies and fish. We found Rbm46 expression was restricted to the mouse germline, detectable in males in the cytoplasm of premeiotic spermatogonia and meiotic spermatocytes. To define its requirement for spermatogenesis, we generated Rbm46 knockout (KO, Rbm46-/-) mice; although male Rbm46-/- mice were viable and appeared grossly normal, they were infertile. Testes from adult Rbm46-/- mice were small, with seminiferous tubules containing only Sertoli cells and few undifferentiated spermatogonia. Using genome-wide unbiased high throughput assays RNA-seq and ‘enhanced crosslinking immunoprecipitation’ coupled with RNA-seq (eCLIP-seq), we discovered RBM46 could bind, via a U-rich conserved consensus sequence, to a cohort of mRNAs encoding proteins required for completion of differentiation and subsequent meiotic initiation. In summary, our studies support an essential role for RBM46 in regulating target mRNAs during spermatogonia differentiation prior to the commitment to meiosis in mice.
Male fertility relies upon continuous daily production of millions of fertilization-competent sperm. These sperm are created in the testis during spermatogenesis, the developmental program founded upon spermatogonial stem cells (SSCs). SSCs divide to produce progeny spermatogonia that either remain in the stem cell pool or commit to differentiate and enter meiosis and ultimately form sperm. The balance between stem cell self-renewal and production of gametes is controlled by changes in the expression of a large complement of genes. An emerging concept in control over gene expression is the essential role of proteins that bind to mRNAs and regulate their stability, storage, and/or translation into proteins. Here, we identify such an RNA binding protein–RBM46 –that is only expressed in the male and female germline and required for gamete production and thus fertility in both sexes. In male mice with a specific deletion of Rbm46, spermatogenesis is arrested at spermatogonial differentiation. RBM46 binds a specific cohort of mRNAs encoding factors essential for differentiation and meiosis, and is thus positioned to play a critical role in post-transcriptional control over gene expression in mammalian spermatogonia.
Citation: Peart NJ, Johnson TA, Lee S, Sears MJ, Yang F, Quesnel-Vallières M, et al. (2022) The germ cell-specific RNA binding protein RBM46 is essential for spermatogonial differentiation in mice. PLoS Genet 18(9): e1010416. https://doi.org/10.1371/journal.pgen.1010416
Editor: Wei Yan, The Lundquist Institute, UNITED STATES
Received: May 16, 2022; Accepted: September 7, 2022; Published: September 21, 2022
Copyright: © 2022 Peart et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: All relevant data are within the manuscript and its Supporting Information files.
Funding: This work was supported by a University of Pennsylvania University Research Foundation (URF) Award (to R.P.C.) and grants from the National Institutes of Health (HG006892 to R.P.C, HD090083 and HD105963 to C.B.G.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The foundation of mammalian spermatogenesis is provided by the regenerative pool of spermatogonial stem cells (SSCs). SSCs are dispersed throughout the normal testis and, upon division, progeny of SSCs either replenish the SSC pool or proliferate as transit-amplifying undifferentiated progenitor spermatogonia. These progenitor spermatogonia commit to meiosis by differentiating in response to retinoic acid (RA). The essential differentiation program in the mouse lasts 8.6 days, culminating in entry into meiosis as preleptotene spermatocytes. Disruption of spermatogonial fate diminishes male fertility by ultimately impairing sperm production; indeed, a block in differentiation of undifferentiated spermatogonia results in maturation arrest, while overactive differentiation can lead to eventual germline loss. Spermatogonia that commit to the lengthy differentiation program have but two fates–either initiating meiosis as spermatocytes or dying by apoptosis. Indeed, we are unaware of any pharmacologic-treated or mutant or knockout (KO) mouse models with testes containing stable populations of differentiating spermatogonia. Despite the critical nature of the differentiation program, the underlying molecular mechanisms remain largely undefined. One reason for this is the relative paucity of transcriptome-wide changes [1–5]. In line with this, recent studies from our lab revealed RA activates the ‘mammalian target of rapamycin complex 1’ (mTORC1) kinase signaling complex, leading to enhanced translation of differentiation-required proteins such as KIT, STRA8, and SOHLH1/2 [6–9]. Taken together, this reveals a critical reliance upon post-transcriptional control mechanisms for gene regulation during spermatogonial differentiation.
Gene expression can be profoundly controlled at the post-transcriptional level, by regulating pre-mRNA splicing, polyadenylation, mRNA stability, translation, and/or localization [10–12]. These regulatory events are largely directed by sequence-specific RNA binding proteins (RBPs). RBPs are expressed in many tissues and cell types, but male germ cells express an especially high number of unique RBPs. Exemplary germ cell specific RBPs include MSY2, DAZL, BOLL, NANOS2, NANOS3, PIWIL1, DND1, RBMXL2, and DDX4, all of which play essential roles during spermatogenesis, as evidenced by mouse KO studies [13–19]. These RBPs have specialized functions at distinct steps of spermatogenesis, indicating the critical importance of RBPs in regulating gene expression to ensure maintenance of male fertility.
While performing a functional screen for a collection of cDNAs, we observed mRNAs encoding the predicted RBP RBM46 were restricted to testes in mouse and human transcriptome databases . Based on this highly restricted expression pattern, we predicted an essential role for RBM46 in spermatogenesis. To test this hypothesis, we generated Rbm46 KO (Rbm46-/-) male mice and discovered loss of RBM46 blocked the completion of spermatogonia differentiation, preventing sperm formation and resulting in infertility. The results presented here position RBM46 as a critical regulator of post-transcriptional gene expression in differentiating mammalian spermatogonia that is essential for completion of spermatogenesis and male fertility.
RBM46 is expressed specifically in spermatogonia and spermatocytes in mouse testes
In a search for novel RBPs expressed in the male germline, we identified a putative candidate encoded by the Rbm46 gene that was testes-specific in transcriptomic datasets . Analysis of single cell RNA-seq data  revealed Rbm46 mRNAs were detectable in adult testes in undifferentiated and differentiating premeiotic spermatogonia, increased in preleptotene, leptotene/zygotene, and pachytene meiotic spermatocytes as well as secondary spermatocytes, declined in early postmeiotic round spermatids, and were undetectable in mid- and late round spermatids, as well as somatic cells of the testis (Fig 1A). We next sought to define the expression pattern of RBM46 protein in mouse testes. Since none of the commercially available antibodies yielded consistent results in immunostaining, CRISPR/Cas9 technology was used to generate mice with tandem copies of the FLAG epitope tag inserted at the N-terminus of RBM46 (Fig 1B). Male mice with homozygous insertion of sequences encoding the FLAG tag (Rbm46FLAG/FLAG) appeared normal and were fertile; their histologically normal testes (S1 Fig) suggested the FLAG tag did not adversely affect RBM46 function. Immunostaining these adult testes using anti-FLAG antibodies revealed RBM46 protein was specifically expressed in cytoplasm of undifferentiated and differentiating spermatogonia as well as spermatocytes, but not in spermatids, sperm, or somatic cells (Fig 1C–1G).
(A) Violin plots showing relative mRNA levels from single cell (sc)RNA-seq data from adult mouse testes . Undiff = undifferentiated; diff = differentiating; spg = spermatogonia; lep/zyg = leptotene + zygotene; pach = pachytene; spc = spermatocyte; tid = spermatid; MΦ = macrophage; PV = perivascular; PTM = peritubular myoid. (B) Diagram depicting insertion of the 2x FLAG tag upstream of exon 2 of the genomic Rbm46 locus. (C-G) IIF was performed to localize the RBM46-FLAG (green in C, red in D-G) in testes from adult Rbm46FLAG/FLAG mice. (C) RBM46-FLAG (green) was detectable in germ cells but not in GATA4+ (red) Sertoli cells. (D-G) RBM46-FLAG (red) was faintly detectable in ZBTB16+ (green) undifferentiated spermatogonia and KIT+ (green) differentiating spermatogonia, indicated by white arrows. Insets in D-E are single fluorescent channel images of individual ZBTB16+ undifferentiated and chains of KIT+ differentiating spermatogonia (in white), respectively. RBM46-FLAG (red) became readily detectable in SYCP3+ (green) spermatocytes (white arrows = pachytene, yellow = leptotene) and was undetectable in lectin+ (green) spermatids (white arrows). Nuclei were stained with DAPI (blue). Scale bars = 25 μm.
RBM46 is essential for fertility in both sexes
To define the requirement for the RNA binding protein RBM46 in spermatogenesis, CRISPR/Cas9 technology was used to generate Rbm46-/- mice. A founder male was identified with a frameshifting deletion between exons 2–3 (Fig 2A). This frameshift in the region encoding the first RNA Recognition Motif (RRM) led to a premature termination codon that disrupted all three consensus RRMs, giving high confidence for a functional null allele (S2A–S2C Fig). Rbm46-/- mice were viable, healthy, and displayed no overt defects (not shown). However, neither Rbm46-/- male nor female mice were able to produce pups when mated with WT counterparts, revealing a requirement for RBM46 in fertility. Compared to WT littermates, adult Rbm46-/- ovaries lacked oocytes, revealing complete loss of the germline (S3A and S3B Fig).
(A) Diagram showing the genomic Rbm46 locus and the cut sites for deletion within exons 2–3. (B-C) Testes from Rbm46-/- mice were significantly smaller than those from Rbm46+/+ littermate controls. (D-E) PAS-stained Bouin’s-fixed testes from adult Rbm46+/- (D) and Rbm46-/- (E) mice, respectively. In contrast to control (D), Rbm46-/- seminiferous epithelia (E) exhibited a single cellular layer, which included apparent Sertoli cells (white arrows) and spermatogonia (yellow arrows). (F-I) IIF was done to confirm the cellular identity of remaining cells in Rbm46-/- seminiferous epithelia. Compared to controls (F), Rbm46-/- seminiferous epithelia (G) contained ZBTB16+ (green) undifferentiated spermatogonia (in tubules marked with an asterisk), and germ cells were immunostained for the pan germ cell marker TRA98 (red). Compared to controls (H), some spermatogonia (indicated by white arrows) in Rbm46-/- testes were STRA8+ (green, I), indicating response to RA, but none were KIT+ (red, I), revealing impaired differentiation. Interstitial cells (marked by yellow arrows, shown in H-I) are always KIT+. Nuclei were stained with DAPI (blue). Triple asterisks indicate statistical significance at P<0.001. Scale bars = 50 μm.
In this study, we focused on the male infertility phenotype. Paired testis weights of Rbm46-/- mice were considerably lower (42.5 ± 13.0 mg) than those from Rbm46+/+ (255.5 ± 67.9 mg) and Rbm46+/- (227 ± 55.9 mg) littermate controls (Fig 2B). This dramatic decrease in Rbm46-/- testis size (Fig 2C) suggested impaired spermatogenesis. Indeed, histological analysis confirmed that, as compared to Rbm46+/+ and Rbm46+/- testes (which appeared normal, e.g., Fig 2C and 2D), Rbm46-/- testes had severe defects in spermatogenesis, with seminiferous tubules containing Sertoli cells and only a few apparent spermatogonia, but lacking spermatocytes, spermatids, or testicular sperm (Fig 2E). This result was confirmed by staining adult testes for the pan germ cell marker TRA98 (also termed GCNA [18, 22, 23] along with the somatic Sertoli cell marker GATA4 (S4A and S4B Fig). In comparison to normal-appearing Rbm46+/- testes (Fig 2F), most tubules in Rbm46-/- adult mice lacked germ cells, although there were isolated populations of ZBTB16+/TRA98+ undifferentiated spermatogonia (Fig 2G). To confirm the absence of more advanced germ cells in Rbm46-/- testes, co-immunostaining was done to detect differentiating spermatogonia markers KIT and STRA8, the latter of which is also highly expressed in preleptotene spermatocytes [24, 25]. As expected, tubule cross sections in control testes contained numerous KIT+ differentiating spermatogonia and STRA8+ preleptotene spermatocytes (Fig 2H). In Rbm46-/- testes, some TRA98+ spermatogonia were also STRA8+, revealing the capacity to respond to RA; however, none were KIT+ (Figs 2I and S4F), suggesting an inability to undergo bona fide RA-induced differentiation. In agreement with this, there were no SYCP3+ meiotic spermatocytes in Rbm46-/- testes, in contrast to controls (S4C–S4F Fig). Taken together, these findings suggest impaired spermatogonial differentiation and reveal an absence of meiotic spermatocytes in Rbm46-/- testes.
Although Rbm46 mRNA and protein were detectable primarily in germ cells, we tested the cell-autonomous requirement by generating germ cell-specific conditional KO mice. These mice were created by crossing Rbm46fl/fl and Stra8-iCre, the latter of which is expressed beginning in undifferentiated progenitor spermatogonia . The testis phenotype of adult Rbm46fl/-;Stra8-Cre mice (S5 Fig) was indistinguishable from those with conventional whole-body deletion (Fig 2), confirming an essential cell autonomous role for RBM46 during male germ cell development.
Spermatogonial differentiation is impaired in developing Rbm46-/- testes
We next sought to precisely define the onset of the spermatogenic defect in Rbm46-/- testes. To accomplish this, we examined Rbm46-/- testes during the well-characterized first wave of spermatogenesis, when populations of progressively advanced germ cells predictably appear on successive days . In control testes, at P6, 8, 10, 15, and 21 the most advanced germ cell types were differentiating spermatogonia, preleptotene spermatocytes, leptotene spermatocytes, pachytene spermatocytes, and round spermatids, as expected  (Fig 3A–3E). In stark contrast, Rbm46-/- testes only contained apparent spermatogonia on each of these days (Fig 3F–3J, 3K and 3L), and there was no difference in numbers of spermatogonia as early as P6 (S6 Fig).
(A-E) The first cohort of spermatogenic cells appear on predictable days during the first wave of spermatogenesis in control PAS-stained Rbm46+/+ testes. These include spermatogonia (red arrows), preleptotene spermatocytes (orange arrows), leptotene spermatocytes (yellow arrows), pachytene spermatocytes (green arrows), and round spermatids (blue arrows). (F-J) In contrast, Rbm46-/- testes contained only spermatogonia (red arrows) and apparently degenerating germ cells (grey arrows). Meiotic spermatocytes were not observed at any age examined. (K-L) P8 testes contain abundant TRA98+ (brown) spermatogonia in both control and Rbm46-/- testes. (M-O) P10, P15, and P21 Rbm46-/- testes contained TRA98+ (brown) basally located spermatogonia. Scale bars = 50 μm.
To confirm the identity of the resident germ cells in developing Rbm46-/- testes, we performed immunostaining for the bona fide spermatogonia differentiation protein marker KIT, which also is expressed in somatic cells in the interstitial compartment [29–34]. At P8, 10, 15, and 21 KIT was readily detectable in the membrane of differentiating spermatogonia, as expected (Fig 4A–4D). In Rbm46-/- testes, significantly fewer KIT+ spermatogonia were present at each of these ages, with numbers remaining stagnant as the mice age (Fig 4E–4I). Thus, we conclude that although spermatogonia initiated the program of differentiation, it was not sustained, leading to stalled germ cell development and an absence of meiotic cells.
(A-D) Darkly staining KIT+ (brown) spermatogonia (gray arrows, A) were numerous in Rbm46+/+ testes. KIT+ interstitial cells are indicated with white arrows. (E-H) At P8, there are scattered faintly staining KIT+ spermatogonia (gray arrows, E), and fewer are seen afterwards, from P10 (F) through P15 (G) and then at P21 (H). (I) Quantitation of KIT+ spermatogonia at each age. Scale bar = 50 μm. Triple asterisks indicate statistical significance at P<0.001.
RBM46 is required for activation of differentiation- and meiosis-associated gene expression in spermatogonia
To begin to define underlying molecular defects in Rbm46-/- spermatogonia, we performed bulk RNA-Seq on WT and Rbm46-/- testes from P8 mice. This age was selected for analysis as it represented a time that, although there was some germ cell degeneration in Rbm46-/- testes (Fig 3G), WT and Rbm46-/- testes had similar apparent numbers of germ cells (Fig 3K–3L). Quantitation revealed a ~21% decrease in numbers of TRA98+ germ cells in Rbm46-/- testes. We reasoned differences in gene expression at the mRNA level would reveal key dysregulated genes due to either direct regulation by RBM46 on mRNA stability or indirect downstream consequences of Rbm46 deletion. We used DESeq2 to identify differences in mRNA levels between WT and Rbm46-/- testes. For protein coding genes (using a cutoff adjusted p-value <0.05), we identified 561 upregulated and 1,218 downregulated transcripts (S1 Table). Changes in mRNA abundance were modest, with only 167 downregulated genes and 33 upregulated genes showing >2-fold changes (Fig 5A). Gene ontology (GO) analysis of downregulated genes identified numerous terms relevant to spermatogenesis, including several related to meiosis: ‘spermatogenesis’, ‘synapsis’, ‘male gamete generation’, and ‘synaptonemal complex assembly’ (Fig 5B). Examples of meiotic genes with reduced mRNA levels in Rbm46-/- testes included Dmc1, H2afx, Meiob, Spo11, Mov10l, Hormad1, Sycp2, and Sycp3. We also identified reduced levels of several mRNAs encoding proteins involved with (e.g., Stra8) or required for (e.g., Kit, Sohlh1; Fig 5C) spermatogonia differentiation. There were no significant changes in mRNA levels of most markers of undifferentiated spermatogonia (e.g., Gfra1, Id4, Nanos2/3, Cdh1, Ret, Itga6, Itgb1, and Sall4). GO analysis of upregulated genes did not identify terms with apparent relevance to spermatogenesis (Fig 5B). We did, however, note increased levels of somatic cell markers (e.g., Sertoli cell mRNAs Sox9 and Clu and Leydig cell markers Cyp17a1, Hmgcs2, and Prlr [21, 35, 36]). Using the Majiq computational pipeline , we only found few changes in alternative splicing (see S2 Table) and, although the splicing differences were important (S7A Fig) and mostly involved alternative first or last exon events (S7A Fig), all but eight genes (Lrif1, Apobec3, Zfp429, Chd1l, Prickle2, Selenbp2, Zfp697, Ndufs1) affected at the splicing level were unaffected at the level of mRNA abundance. Genes with differential splicing were not enriched for any specific GO term. In summary, there was an apparent decrease in the mRNA abundance of genes encoding proteins required for spermatogonial differentiation and meiosis, which is likely due to indirect action of RBM46, in that differentiating spermatogonia and preleptotene spermatocytes were absent in P8 Rbm46-/- testes.
(A) Heatmap of 200 genes with >2-fold changes in Rbm46-/- relative to Rbm46+/+ controls at P8. (B) Gene ontology terms for biological processes enriched in genes that are downregulated (top) or upregulated (bottom) in Rbm46-/-. Circle size and numbers correspond to the number of genes that are differentially expressed and represented in a GO term over to the total number of genes listed in the GO term. (C) Expression level of genes involved in spermatogonia differentiation and somatic cell markers. P-values are DESeq2 adjusted p-values comparing Rbm46+/+ to Rbm46-/- testes.
RBM46-bound mRNAs are enriched for functions in RNA processing, meiosis, and translation regulation
To identify mRNAs directly bound by RBM46 in the male germline, we used enhanced crosslinking coupled with immunoprecipitation and RNA-seq (eCLIP-Seq). This method provides unbiased genome-wide coverage from small amounts of cellular input, enabling identification of RBP binding sites at single nucleotide resolution . We used testes from Rbm46FLAG/FLAG mice (Fig 1B), as the FLAG-tagged RBM46 protein can be efficiently and specifically immunoprecipitated using FLAG antibodies. Because RBM46 is expressed in both spermatogonia and spermatocytes (Fig 1D–1F), we used eCLIP in testes from RBM46FLAG/FLAG mice at P21, an age when they contain spermatogonia, spermatocytes, and the very first emergent round spermatids . Immunoprecipitated material was separated by electrophoresis, transferred to a nitrocellulose membrane, and the region containing crosslinked RNAs excised and released from the membrane (Figs 6A and S8). eCLIP libraries were prepared and five replicate eCLIP samples were sequenced with corresponding inputs, processed, and mapped at ~8 x 106 non-redundant reads to the genome (mm10) . We anticipated enrichment of binding sites in mRNA 3’ untranslated regions (3’ UTRs), similar to reports of other cytoplasmic RBPs in male germ cells [40–43]. To our surprise, nearly equal percentages of CLIP tags were present in the 3’ UTR and protein coding sequences, though when corrected for the percentage of these regions in the transcriptome there was a modest enrichment of binding sites in the 3’ UTR over the CDS (Fig 6B). CLIP tags also showed a relatively uniform distribution across mRNAs (Fig 6C).
(A) FLAG-stained western blot of lysates from Rbm46WT/WT and Rbm46FLAG/FLAG showing input and immunoprecipitated proteins, respectively. Band at the expected protein size is indicated by arrow. (B) Genomic distribution of RBM46 binding in the testis, displayed as percentages of binding to each sequence feature. (C) Representative UCSC genome browser view showing exonic binding of RBM46 across Dazl and Meioc. (D) WebLogo showing U-rich RNA binding sequence motif identified using mCross as the most enriched sequence at the RBM46 crosslink sites. (E) Gene ontology analysis performed using EnrichR showing the top biological processes of RBM46-bound mRNAs.
To determine the binding specificity of RBM46, we extracted sequences around CITS and performed de novo motif discovery using mCross, an algorithm developed to simultaneously model RBP binding specificity and the crosslink position in the binding motif . After pooling all replicates, mCross was used extract sequences around crosslink-induced truncation sites for de novo motif discovery, which identified 90,243 crosslink-induced truncation sites (CITS, P<0.001) [39, 45]. This analysis revealed a U-rich motif with a UGAU core and predominant crosslinking at the U1 position of the core (Fig 6D). The UGAU motif is highly enriched at the crosslink sites (with a 25-fold enrichment for crosslinking at U1 of the UGAU motif), while a moderate enrichment was observed in regions around CLIP tag peaks. Given the high signal-to-noise ratio of CITS, we identified a stringent subset of RBM46 target transcripts based on the presence of CITS satisfying two criteria: 1) presence of the UGAU motif with crosslinking at the U1 position; and 2) ≥50 putative truncated tags at the crosslink sites. This allowed us to identify 1,349 CITS associated with 873 unique genes. Gene Ontology (GO) analysis of these genes was performed using DAVID . RBM46 target transcripts were enriched for terms relevant to spermatogenesis using GO analysis (Fig 6E). Of note, there was significant enrichment of genes involved in RNA processing that included several RBPs with functions in spermatogenesis (e.g., DAZL, BOLL, PABPC1, CELF1, CEBP1, PTBP2, and RBM46 itself) and translation initiation factors (e.g., EIF1A, EIF2S1, EIF4G1, and EIF4G2). RBM46 also showed enriched binding to mRNAs encoding essential meiosis proteins (e.g., SYCP1, SYCP2, SYCP3, MEIOC, SPO11, TEX15, HORMAD1, HSPA2, and BRCA2). A list of mRNAs that were bound by RBM46 at P21 and exhibited differential abundance in P8 Rbm46-/- testes are presented in S3 Table.
Here, we localized the germ cell specific RBP RBM46 to the cytoplasm of spermatogonia and spermatocytes, but not in other testes germ cell types nor in somatic cells. We generated KO mice and discovered a germ cell autonomous requirement for RBM46 in spermatogenesis and male fertility. Specifically, RBM46 was essential, in spermatogonia, to complete differentiation in both developing and adult testes. Rbm46 KO testes had altered transcriptomes, with downregulation of transcripts encoding differentiation- and meiosis-associated genes. Using enhanced crosslinking immunoprecipitation  followed by binding analysis with the CLIP Tool Kit , we determined RBM46 directly bound, at a U-rich consensus sequence, to mRNAs encoding proteins involved in spermatogenesis as well as in general translation regulation. In summary, RBP46 is required for spermatogonial differentiation and male fertility, and directly binds to mRNAs encoding genes essential for differentiation and meiosis in the male germline.
All stages of spermatogenesis, from survival of prospermatogonia to the maintenance of SSCs to meiosis and spermiogenesis, require post-transcriptional regulation by RBPs. Indeed, a number of essential RBPs have been identified that repress or activate the translation of select mRNAs, including NANOS2, NANOS3, DAZL, TIAR/TIAL1, PIWIL2/MILI, PIWIL4/MIWI2, DDX4/VASA, MSY2, and LIN28A [16, 40, 43, 47–52]. The functions of essential RBPs include regulation of mRNA splicing, polyadenylation, localization, stability/degradation, and translation [53–55]. While the mechanistic functions and global regulatory targets have been identified for multiple essential RBPs, many RBPs necessary for spermatogenesis remain to be defined and characterized. Therefore, identification of a novel RBP and its genome-wide regulatory targets provides new insights into the molecular pathways that control germ cell gene expression during maintenance and differentiation.
Once in the cytoplasm, mRNAs face three possible fates: translation, storage, or degradation. Transit between these fates is well-known to regulate key transitions during male germ cell development [56, 57]. Our RNA-Seq findings offer further support for a spermatogonial differentiation block in Rbm46-/- testes. However, the changes in mRNA levels were rather modest, including markers of differentiated spermatogonia and meiotic genes. The RNA-seq experiment was performed using testes at P8, a time when there was a ~21% decrease in germ cells, notably differentiating spermatogonia and the first emergent preleptotene spermatocytes entering meiosis. The loss of these cells, and their transcriptomes suggest many, if not most changes in transcript levels in Rbm46-/- testes were indirect, due to the differentiation impairment and not due to changes in RNA posttranscriptional control.
RBM46 was recently discovered to be part of a complex containing several essential proteins. These include the disordered protein MEIOC (required for mouse meiosis [58, 59]), the exoribonuclease XRN1 (Drosophila pacman, required for spermatogenesis and male fertility ), and the RNA helicase YTHDC2 (Drosophila bgcn , required for progression through meiosis in the male mouse germline [62–66]). This YTHDC2-containing complex bound in testes containing both spermatogonia and spermatocytes to a canonical U-rich binding motif [67, 68]. This sequence closely resembles the one identified here, in P21 testes, which contain spermatogonia, spermatocytes, and the first emergent spermatids . YTHDC2’s function in gametogenesis was recently shown to be independent of its N6-methyladenosine (m6A)-modified RNA binding [67, 68]; therefore, it is possible that RBM46, as a resident in this RNA management complex, provides additional RNA binding function (allosterically or directly) through the U-rich binding sequence we identified. In Drosophila, Bgcn is required for translation control and expressed in a reciprocal pattern to the Nanos proteins . Based on published reports and the present data, this arrangement appears to be conserved in mice–we discovered a requirement for RBM46 in spermatogonial differentiation, whereas others have shown NANOS2 and NANOS3 are required for SSC maintenance [70–72]. Therefore, RBM46 may aid in target recognition for YTHDC2 functions in translational regulation.
The uniform binding observed across both coding sequences and UTRs of mRNA transcripts is somewhat uncommon among RBPs but resembles the diffuse mRNA binding pattern shown by CLIP of Fragile X mental retardation protein (FMRP) and LIN28A [51, 73]. FMRP is present in actively translating polysomes and regulates translation [74–76]. Similarly, binding of LIN28A across the CDS and UTRs positively regulates translation of mRNAs, including meiotic transcripts in mouse testes [52, 77–79]. Thus, the atypical binding pattern of RBM46 is consistent with or permissive for a role in translation regulation.
RBM46 is a highly conserved RBP whose function has been examined in flies, fish, and now mice. In Drosophila, the mouse ortholog of RBM46 is encoded by the RBP ‘tumorous testis’ (Tut), which is required for spermatogenesis and male fertility . Interestingly, the phenotype of Tut mutant flies is similar to that reported here–germ cell development is blocked at differentiation, and thus contain only undifferentiated spermatogonia. In addition, in zebrafish (Danio rerio), rbm46 is expressed in male germ cells and is required for spermatogonia to enter or progress through meiosis. . Indeed, male rbm46 mutants were sterile, with testes containing only spermatogonia that proliferated into >16 interconnected 3C-4C germ cells, suggesting incorrect meiotic entry. Rbm46-depleted gonads were sex-reversed to testes, and transcriptome analyses revealed many more changes in mRNA abundance (4,436 up and 3,571 down) than we observed here, including reduced levels of many meiotic mRNAs (e.g., spo11, dmc1, rad51, msh4, mlh1, rec8, smc1b, sycp1-3). These findings support a major role in directing meiotic gene expression. Here, we identified numerous mRNAs encoding essential meiosis proteins among the top RBM46 CLIP targets in P21 testes containing a mixture of spermatogonia and spermatocytes (e.g., SYCP1, SYCP2, SYCP3, MEIOC, SPO11, TEX15, HORMAD1, HSPA2, and BRCA2). These findings provide further support for RBM46 functions in meiosis while also suggesting that mRNAs highly bound by RBM46 support translation, or at least do not inhibit it.
In two previous studies from the same research group, a critical role for RBM46 was reported in embryonic stem cell (ESC) and trophectoderm differentiation [82, 83]. These studies found RBM46 promoted Cdx2 mRNA stability and degradation of beta-catenin (Ctnnb1) mRNAs in ESCs. However, the second manuscript was recently retracted by the authors . It is notable that Rbm46 expression is rather low in ESCs in available datasets, suggesting the primary roles of RBM46 are in male germ cell development and function. Furthermore, the fact that male Rbm46-/- mice were otherwise normal, without any phenotypes other than infertility, is not compatible with an essential role of RBM46 outside of the germline.
All animal procedures and experiments were approved by the Institutional Animal Care and Use Committees (IACUC) at the University of Pennsylvania (protocol #803164) and East Carolina University (approval A3469-01).
Rbm46FLAG/FLAG mice and Rbm46-/- mice were generated in the Penn Transgenic and Chimeric Mouse and CRISPR-Cas9 Mouse Targeting Core Facilities (supported by NIH grant P30DK050306). To create Rbm46FLAG/FLAG mice, Alt-R CRISPR-Cas9 crRNA (Integrated DNA Technologies (IDT: Iowa City, IA)) targeting the sequence 5’-ATCAGTGTTTCTTCATTCA-3’ (anti-sense) and a rescue donor oligo were created containing two tandem copies of the FLAG Tag in-frame after the ATG start codon with a 5’ 91 nt homology arm and 3’ 37 nt homology arm. The crRNA and donor oligos were microinjected in fertilized eggs together with an mRNA encoding Cas9 protein.
For Rbm46-/- mice, two crRNAs were generated in vitro using T7 polymerase to target the following sequences in Rbm46 exon 2 (5’- ATGAATGAAGAAAACACTGA-3’ and 5’-ATAATTGTTAAGAATCCGGA-3’ (anti-sense)). The two crRNAs were microinjected together into fertilized eggs along with Cas9 mRNAs. Resulting pups were screened by PCR for heterozygous KI or deletion and founder mice were confirmed by DNA sequencing. Mice were humanely euthanized by CO2 asphyxiation followed by cervical dislocation. Mice were on a B6SJLF1/J hybrid genetic background (strain #100012, The Jackson Laboratory).
Tissue collection, fixation, and immunostaining
For cryosections or paraffin embedding, testes were fixed for 4 hrs–overnight in either fresh 4% paraformaldehyde or Bouin’s solution, respectively, at 4°C and prepared as described previously . Bouin’s-fixed testes were stained with Periodic Acid Schiff (PAS) using standard methods. For immunohistochemistry (IHC), immunostaining was performed on Bouin’s-fixed sections as described . Brightfield images were captured on an Axio Observer A1 inverted microscope outfitted with a Zeiss Axiocam 503 color digital camera and Zen software (Carl Zeiss Microscopy, LLC).
For indirect immunofluorescence (IIF), immunostaining was performed on cryosections as described . Alexa-Fluor conjugated secondary antibodies (Thermo Scientific) raised against the animal host of the primary antibody (Table 1) were incubated for 1 hr at room temperature at a 1:500 dilution. Coverslips were mounted for IIF with Vectastain containing DAPI (Vector Laboratories). Sections were imaged using a Fluoview FV1000 confocal laser scanning confocal microscope (Olympus America).
Testes from P8 mice were flash frozen in liquid nitrogen and ground using a mortar and pestle. Ground tissue was homogenized in TriZol reagent by passing samples through 18- and 26-gauge needles, and RNA was extracted with RNeasy minikit (Qiagen) using manufacturer’s instructions. Total RNA was then submitted to Genewiz and Illumina libraries were prepared after rRNA depletion using the Illumina Ribo-Zero kit. Sequencing was performed using Illumina HiSeq for 150 bp paired end sequencing using four replicates each from wild type control and Rbm46-/- samples. Adapters were trimmed from RNA-Seq samples using BBDuk, aligned to the mouse GRCm38 genome assembly using STAR v.2.5.1B, and sorted and indexed using samtools v.1.9. For gene expression quantification, salmon v.0.14.0 was used in mapping-based mode with selective alignment on trimmed fastq files using GENCODE vM23 annotation to create the index. Differential gene expression analysis was performed with DESeq2 v.1.22.2. Differential splicing analysis was performed with MAJIQ v.2.1 using GENCODE vM23 reference transcriptome annotation without intron retention quantification. We identified differentially spliced junctions by keeping junctions that had a delta PSI of at least 15 with a probability that the delta PSI is above 15 of at least 95%. Gene ontology analysis was performed with enrichR v.1.0 using a 2018 release of the GO Consortium annotations.
Testes were harvested from mice and rinsed in PBS. Testes were detunicated, triturated, dounced in PBS, and tissue material was crosslinked three times at 400 mJ/cm2 using a Stratalinker 2400 (Stratagene). Samples were then flash-frozen in liquid nitrogen and stored at -80°C until use. Each replicate was derived from a pair of testes from a single mouse. Samples were lysed, and crosslinked RNP complexes were treated with 5 U/ml RNAse I, immunoprecipitated, and used to generate eCLIP libraries and control input libraries as previously described . In brief, to extract RBM46-specific interactors, cleared immunoprecipitants were resolved on 4–12% Bis-Tris protein gel and transferred to a nitrocellulose membrane. The RNA:RNP complex was extracted from the nitrocellulose membrane by cutting a region that included the RNA binding protein, RBM46 (size ~62 kDa) and a region of the membrane ~50 kDa above the RBM46 band. The RNA was isolated from the membrane following proteinase K and urea treatments. An Illumina Nova-Seq was used for 50 bp paired end sequencing. Raw data from Rbm46 eCLIP experiments and input controls were processed using CLIP Tool Kit (CTK) . Unique tags were identified after stringent mapping to the reference genome (mm10) and collapsing of PCR duplicates. Only read2, which corresponds to the 5’ end of CLIP tags, was used for analyses.
S1 Fig. Testes from adult Rbm46FLAG/FLAG mice were morphologically normal.
(A-B) Similar to Bouin’s-fixed and PAS-stained testes from adult (P>60) WT (A) mice, those from Rbm46FLAG/FLAG mice (B) contained normal complements of male germ cells (Spg = spermatogonium; Pl = preleptotene spermatocyte; L = leptotene spermatocyte; Z = zygotene spermatocyte; PS = pachytene spermatocyte; RS = round spermatid; ES = elongating spermatid; CS = condensing spermatid; SC = Sertoli cell nucleus) within that appropriate seminiferous tubule stages, indicated on each cross section in Roman numerals. Scale bar = 50 μm.
S2 Fig. Schematic of Rbm46 whole-body and conditional KO alleles.
(A) For whole-body KO allele, the deleted region is indicated by scissors. (B) For conditional KO allele, inserted loxP sites are represented by blue arrows. (C) RBM46 protein contains three RRMs, indicated in yellow.
S3 Fig. Adult Rbm46-/- ovaries lacked a germline.
(A-B) PAS-stained ovaries from Rbm46+/+ and Rbm46-/- mice, with genotypes indicated on each image. The cortex of an Rbm46+/+ ovary (A) contained numerous oocytes (white arrows) in follicles at various stages of development. In contrast, the Rbm46+/+ ovary lacked oocytes or organized follicles (B). Scale bar = 200 μm.
S4 Fig. Adult Rbm46-/- testes contained abundant Sertoli cells but lacked SYCP3+ meiotic spermatocytes.
(A-B) GATA4+ Sertoli cells (green) were present in both Rbm46+/- and Rbm46-/- testes, but there were few TRA98+ (red) germ cells in Rbm46-/- testes. (C-D) In contrast to Rbm46+/- testes, there were no SYCP3+ (green) spermatocytes in Rbm46-/- testes. (E-F) Using Rbm46+/- and Rbm46-/- testes, the numbers of germ cells (E) and % cell fate (F) were quantified. Nuclei were stained with DAPI (blue). Scale bar = 50 μm.
S5 Fig. Conditional deletion of Rbm46 with Stra8-Cre resulted in an adult spermatogenesis phenotype resembling that of whole-body KO mice.
(A-C) Compared to controls, adult conditional KO testes were dramatically reduced in size. (D) Seminiferous epithelia from control mice (left panel) contained Sertoli cells as well as all advanced germ cell types, with examples marked including leptotene (Lep) and pachytene (Pac) spermatocytes as well as elongated spermatids (ES). In stark contrast, seminiferous epithelia of conditional KO testes contained only somatic Sertoli cells and a few spermatogonia (Spg). Scale bar = 50 μm.
S6 Fig. Similar numbers of spermatogonia present in Rbm46+/- and Rbm46-/- testes at P6.
S7 Fig. Differential splicing events in Rbm46-/- testes at P8.
(A) Heatmap depicts 36 splicing events with a change in percent spliced in (PSI) of at least 15% in Rbm46-/- relative to Rbm46+/+ testes at P8. (B) Distribution of the types of altered splicing events in Rbm46-/- testes. Absolute number of changing events for each type shown on the chart. ALE = Alternative Last Exon; AFE = Alternative First Exon; Alt 3 = Alternative 3’ splice site; Alt 5 = Alternative 5’ splice site.
S8 Fig. CLIP of Rbm46FLAG/FLAG in P21 mouse testes.
(A) SDS-PAGE of crosslinked immunoprecipitants and input from Rbm46FLAG/FLAG and Rbm46WT/WT testes. RNAs in the immunoprecipitants were ligated (on beads) with an RNA linker containing the IRDye 800CW fluorochrome to enable RNA visualization. (B) Corresponding anti-FLAG western blot of crosslinked immunoprecipitants and input from Rbm46FLAG/FLAG and Rbm46WT/WT testes following FLAG immunoprecipitation.
S1 Table. List of differentially expressed genes in Rbm46-/- testes at P8.
S2 Table. List of differential splicing events in Rbm46-/- testes at P8.
- 1. Gewiss RL, Shelden EA, Griswold MD. STRA8 induces transcriptional changes in germ cells during spermatogonial development. Mol Reprod Dev. 2021. Epub 2021/01/06. pmid:33400349.
- 2. Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S, et al. A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq. Dev Cell. 2018;46(5):651–67.e10. Epub 2018/08/28. pmid:30146481.
- 3. Grive KJ, Hu Y, Shu E, Grimson A, Elemento O, Grenier JK, et al. Dynamic transcriptome profiles within spermatogonial and spermatocyte populations during postnatal testis maturation revealed by single-cell sequencing. PLoS Genet. 2019;15(3):e1007810. Epub 2019/03/21. pmid:30893341; PubMed Central PMCID: PMC6443194.
- 4. Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC, et al. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. Cell reports. 2018;25(6):1650–67.e8. Epub 2018/11/08. pmid:30404016.
- 5. Hermann BP, Mutoji KN, Velte EK, Ko D, Oatley JM, Geyer CB, et al. Transcriptional and Translational Heterogeneity among Neonatal Mouse Spermatogonia. Biol Reprod. 2015. Epub 2015/01/09. pmid:25568304
- 6. Kirsanov O, Renegar RH, Busada JT, Serra ND, Harrington EV, Johnson TA, et al. The rapamycin analog Everolimus reversibly impairs male germ cell differentiation and fertility in the mousedagger. Biology of reproduction. 2020;103(5):1132–43. pmid:32716476; PubMed Central PMCID: PMC7609841.
- 7. Serra ND, Velte EK, Niedenberger BA, Kirsanov O, Geyer CB. Cell-autonomous requirement for mammalian target of rapamycin (Mtor) in spermatogonial proliferation and differentiation in the mousedagger. Biol Reprod. 2017;96(4):816–28. Epub 2017/04/06. pmid:28379293.
- 8. Busada JT, Niedenberger BA, Velte EK, Keiper BD, Geyer CB. Mammalian target of rapamycin complex 1 (mTORC1) Is required for mouse spermatogonial differentiation in vivo. Dev Biol. 2015;407(1):90–102. pmid:26254600; PubMed Central PMCID: PMC4641790.
- 9. Busada JT, Chappell VA, Niedenberger BA, Kaye EP, Keiper BD, Hogarth CA, et al. Retinoic acid regulates Kit translation during spermatogonial differentiation in the mouse. Dev Biol. 2015;397(1):140–9. Epub 2014/12/03. pmid:25446031
- 10. Lambert NJ, Robertson AD, Burge CB. RNA Bind-n-Seq: Measuring the Binding Affinity Landscape of RNA-Binding Proteins. Methods in enzymology. 2015;558:465–93. pmid:26068750; PubMed Central PMCID: PMC5576890.
- 11. Van Nostrand EL, Freese P, Pratt GA, Wang X, Wei X, Xiao R, et al. A large-scale binding and functional map of human RNA-binding proteins. Nature. 2020;583(7818):711–9. pmid:32728246; PubMed Central PMCID: PMC7410833.
- 12. Gerstberger S, Hafner M, Tuschl T. A census of human RNA-binding proteins. Nat Rev Genet. 2014;15(12):829–45. pmid:25365966.
- 13. Yang J, Morales CR, Medvedev S, Schultz RM, Hecht NB. In the absence of the mouse DNA/RNA-binding protein MSY2, messenger RNA instability leads to spermatogenic arrest. Biology of reproduction. 2007;76(1):48–54. pmid:17035640.
- 14. Tsuda M, Sasaoka Y, Kiso M, Abe K, Haraguchi S, Kobayashi S, et al. Conserved role of nanos proteins in germ cell development. Science. 2003;301(5637):1239–41. Epub 2003/08/30. pmid:12947200.
- 15. Suzuki A, Niimi Y, Shinmyozu K, Zhou Z, Kiso M, Saga Y. Dead end1 is an essential partner of NANOS2 for selective binding of target RNAs in male germ cell development. EMBO Rep. 2016;17(1):37–46. pmid:26589352; PubMed Central PMCID: PMC4718414.
- 16. Ruggiu M, Speed R, Taggart M, McKay SJ, Kilanowski F, Saunders P, et al. The mouse Dazla gene encodes a cytoplasmic protein essential for gametogenesis. Nature. 1997;389(6646):73–7. Epub 1997/09/04. pmid:9288969.
- 17. Ehrmann I, Crichton JH, Gazzara MR, James K, Liu Y, Grellscheid SN, et al. An ancient germ cell-specific RNA-binding protein protects the germline from cryptic splice site poisoning. Elife. 2019;8. pmid:30674417; PubMed Central PMCID: PMC6345566.
- 18. Tanaka SS, Toyooka Y, Akasu R, Katoh-Fukui Y, Nakahara Y, Suzuki R, et al. The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Genes Dev. 2000;14(7):841–53. Epub 2000/04/15. pmid:10766740; PubMed Central PMCID: PMC316497.
- 19. Carpinelli MR, de Vries ME, Auden A, Butt T, Deng Z, Partridge DD, et al. Inactivation of Zeb1 in GRHL2-deficient mouse embryos rescues mid-gestation viability and secondary palate closure. Disease models & mechanisms. 2020;13(3). pmid:32005677; PubMed Central PMCID: PMC7104862.
- 20. Su AI, Wiltshire T, Batalov S, Lapp H, Ching KA, Block D, et al. A gene atlas of the mouse and human protein-encoding transcriptomes. Proc Natl Acad Sci U S A. 2004;101(16):6062–7. pmid:15075390; PubMed Central PMCID: PMC395923.
- 21. Hermann BP, Cheng K, Singh A, Roa-De La Cruz L, Mutoji KN, Chen IC, et al. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids. Cell Rep. 2018;25(6):1650–67 e8. pmid:30404016; PubMed Central PMCID: PMC6384825.
- 22. Enders GC, May JJ, 2nd. Developmentally regulated expression of a mouse germ cell nuclear antigen examined from embryonic day 11 to adult in male and female mice. Dev Biol. 1994;163(2):331–40. pmid:8200475.
- 23. Carmell MA, Dokshin GA, Skaletsky H, Hu YC, van Wolfswinkel JC, Igarashi KJ, et al. A widely employed germ cell marker is an ancient disordered protein with reproductive functions in diverse eukaryotes. Elife. 2016;5. pmid:27718356; PubMed Central PMCID: PMC5098910.
- 24. Oulad-Abdelghani M, Bouillet P, Decimo D, Gansmuller A, Heyberger S, Dolle P, et al. Characterization of a premeiotic germ cell-specific cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene. J Cell Biol. 1996;135(2):469–77. Epub 1996/10/01. pmid:8896602; PubMed Central PMCID: PMC2121034.
- 25. Zhou Q, Nie R, Li Y, Friel P, Mitchell D, Hess RA, et al. Expression of stimulated by retinoic acid gene 8 (Stra8) in spermatogenic cells induced by retinoic acid: an in vivo study in vitamin A-sufficient postnatal murine testes. Biol Reprod. 2008;79(1):35–42. Epub 2008/03/07. pmid:18322276.
- 26. Sadate-Ngatchou PI, Payne CJ, Dearth AT, Braun RE. Cre recombinase activity specific to postnatal, premeiotic male germ cells in transgenic mice. Genesis (New York, NY: 2000). 2008;46(12):738–42. pmid:18850594; PubMed Central PMCID: PMC2837914.
- 27. Geyer CB. Setting the stage: the first round of spermatogenesis. In: Oatley JM, Griswold MD, editors. The Biology of Mammalian Spermatogonia. New York: Springer; 2017. p. 39–63.
- 28. Bellve AR, Cavicchia JC, Millette CF, O’Brien DA, Bhatnagar YM, Dym M. Spermatogenic cells of the prepuberal mouse. Isolation and morphological characterization. J Cell Biol. 1977;74(1):68–85. Epub 1977/07/01. PubMed Central PMCID: PMC2109873. pmid:874003
- 29. Manova K, Nocka K, Besmer P, Bachvarova RF. Gonadal expression of c-kit encoded at the W locus of the mouse. Development. 1990;110(4):1057–69. Epub 1990/12/01. pmid:1712701.
- 30. Sorrentino V, Giorgi M, Geremia R, Besmer P, Rossi P. Expression of the c-kit proto-oncogene in the murine male germ cells. Oncogene. 1991;6(1):149–51. Epub 1991/01/01. pmid:1704118.
- 31. Yoshinaga K, Nishikawa S, Ogawa M, Hayashi S, Kunisada T, Fujimoto T. Role of c-kit in mouse spermatogenesis: identification of spermatogonia as a specific site of c-kit expression and function. Development. 1991;113(2):689–99. Epub 1991/10/01. pmid:1723681.
- 32. Packer AI, Besmer P, Bachvarova RF. Kit ligand mediates survival of type A spermatogonia and dividing spermatocytes in postnatal mouse testes. Mol Reprod Dev. 1995;42(3):303–10. Epub 1995/11/01. pmid:8579844.
- 33. Schrans-Stassen BH, van de Kant HJ, de Rooij DG, van Pelt AM. Differential expression of c-kit in mouse undifferentiated and differentiating type A spermatogonia. Endocrinology. 1999;140(12):5894–900. Epub 1999/12/01. pmid:10579355.
- 34. Kissel H, Timokhina I, Hardy MP, Rothschild G, Tajima Y, Soares V, et al. Point mutation in kit receptor tyrosine kinase reveals essential roles for kit signaling in spermatogenesis and oogenesis without affecting other kit responses. EMBO J. 2000;19(6):1312–26. Epub 2000/03/16. pmid:10716931; PubMed Central PMCID: PMC305672.
- 35. Ernst C, Eling N, Martinez-Jimenez CP, Marioni JC, Odom DT. Staged developmental mapping and X chromosome transcriptional dynamics during mouse spermatogenesis. Nature communications. 2019;10(1):1251. pmid:30890697; PubMed Central PMCID: PMC6424977.
- 36. Green CD, Ma Q, Manske GL, Shami AN, Zheng X, Marini S, et al. A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq. Dev Cell. 2018;46(5):651–67 e10. pmid:30146481; PubMed Central PMCID: PMC6713459.
- 37. Vaquero-Garcia J, Barrera A, Gazzara MR, Gonzalez-Vallinas J, Lahens NF, Hogenesch JB, et al. A new view of transcriptome complexity and regulation through the lens of local splicing variations. Elife. 2016;5:e11752. pmid:26829591; PubMed Central PMCID: PMC4801060.
- 38. Van Nostrand EL, Pratt GA, Shishkin AA, Gelboin-Burkhart C, Fang MY, Sundararaman B, et al. Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP). Nature methods. 2016;13(6):508–14. Epub 2016/03/29. pmid:27018577; PubMed Central PMCID: PMC4887338.
- 39. Shah A, Qian Y, Weyn-Vanhentenryck SM, Zhang C. CLIP Tool Kit (CTK): a flexible and robust pipeline to analyze CLIP sequencing data. Bioinformatics. 2017;33(4):566–7. pmid:27797762; PubMed Central PMCID: PMC6041811.
- 40. Li H, Liang Z, Yang J, Wang D, Wang H, Zhu M, et al. DAZL is a master translational regulator of murine spermatogenesis. National science review. 2019;6(3):455–68. pmid:31355046; PubMed Central PMCID: PMC6660020.
- 41. Zagore LL, Sweet TJ, Hannigan MM, Weyn-Vanhentenryck SM, Jobava R, Hatzoglou M, et al. DAZL Regulates Germ Cell Survival through a Network of PolyA-Proximal mRNA Interactions. Cell Rep. 2018;25(5):1225–40 e6. pmid:30380414; PubMed Central PMCID: PMC6878787.
- 42. Yamaji M, Jishage M, Meyer C, Suryawanshi H, Der E, Yamaji M, et al. DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs. Nature. 2017;543(7646):568–72. pmid:28297718; PubMed Central PMCID: PMC5488729.
- 43. Mikedis MM, Fan Y, Nicholls PK, Endo T, Jackson EK, Cobb SA, et al. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors. Elife. 2020;9. pmid:32686646; PubMed Central PMCID: PMC7445011.
- 44. Feng H, Bao S, Rahman MA, Weyn-Vanhentenryck SM, Khan A, Wong J, et al. Modeling RNA-Binding Protein Specificity In Vivo by Precisely Registering Protein-RNA Crosslink Sites. Mol Cell. 2019;74(6):1189–204 e6. pmid:31226278; PubMed Central PMCID: PMC6676488.
- 45. Weyn-Vanhentenryck Sebastien M, Mele A, Yan Q, Sun S, Farny N, Zhang Z, et al. HITS-CLIP and Integrative Modeling Define the Rbfox Splicing-Regulatory Network Linked to Brain Development and Autism. Cell Reports. 2014;6(6):1139–52. pmid:24613350
- 46. Huang DW, Sherman BT, Tan Q, Kir J, Liu D, Bryant D, et al. DAVID Bioinformatics Resources: expanded annotation database and novel algorithms to better extract biology from large gene lists. Nucleic Acids Res. 2007;35(Web Server issue):W169–75. pmid:17576678; PubMed Central PMCID: PMC1933169.
- 47. Beck AR, Miller IJ, Anderson P. RNA binding protein TIAR is essential for primordial germ cell development. Proc Natl Acad Sci U S A. 1998;95:2331–6. pmid:9482885
- 48. Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita Y, et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development. 2004;131(4):839–49. Epub 2004/01/23. pmid:14736746.
- 49. Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Takamatsu K, Chuma S, Kojima-Kita K, et al. MVH in piRNA processing and gene silencing of retrotransposons. Genes Dev. 2010;24(9):887–92. Epub 2010/05/05. pmid:20439430; PubMed Central PMCID: PMC2861188.
- 50. Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A, Ikawa M, et al. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev. 2008;22(7):908–17. Epub 2008/04/03. pmid:18381894; PubMed Central PMCID: PMC2279202.
- 51. Mayr F, Heinemann U. Mechanisms of Lin28-mediated miRNA and mRNA regulation—a structural and functional perspective. Int J Mol Sci. 2013;14(8):16532–53. pmid:23939427; PubMed Central PMCID: PMC3759924.
- 52. Wang M, Yu L, Wang S, Yang F, Wang M, Li L, et al. LIN28A binds to meiotic gene transcripts and modulates their translation in male germ cells. J Cell Sci. 2020;133(12). pmid:32376786.
- 53. Licatalosi DD. Roles of RNA-binding Proteins and Post-transcriptional Regulation in Driving Male Germ Cell Development in the Mouse. Advances in experimental medicine and biology. 2016;907:123–51. pmid:27256385; PubMed Central PMCID: PMC6219387.
- 54. Legrand JMD, Hobbs RM. RNA processing in the male germline: Mechanisms and implications for fertility. Seminars in cell & developmental biology. 2018;79:80–91. pmid:29024760.
- 55. Paronetto MP, Sette C. Role of RNA-binding proteins in mammalian spermatogenesis. Int J Androl. 2010;33(1):2–12. Epub 2009/03/14. pmid:19281489
- 56. Brengues M, Teixeira D, Parker R. Movement of eukaryotic mRNAs between polysomes and cytoplasmic processing bodies. Science. 2005;310(5747):486–9. Epub 2005/09/06. pmid:16141371; PubMed Central PMCID: PMC1863069.
- 57. Keiper BD, Rhoads RE. Translational recruitment of Xenopus maternal mRNAs in response to poly(A) elongation requires initiation factor eIF4G-1. Dev Biol. 1999;206(1):1–14. Epub 1999/01/27. pmid:9918691.
- 58. Soh YQS, Mikedis MM, Kojima M, Godfrey AK, de Rooij DG, Page DC. Meioc maintains an extended meiotic prophase I in mice. PLoS Genet. 2017;13(4):e1006704. Epub 2017/04/06. pmid:28380054; PubMed Central PMCID: PMC5397071.
- 59. Abby E, Tourpin S, Ribeiro J, Daniel K, Messiaen S, Moison D, et al. Implementation of meiosis prophase I programme requires a conserved retinoid-independent stabilizer of meiotic transcripts. Nat Commun. 2016;7:10324. Epub 20160108. pmid:26742488; PubMed Central PMCID: PMC4729902.
- 60. Zabolotskaya MV, Grima DP, Lin MD, Chou TB, Newbury SF. The 5’-3’ exoribonuclease Pacman is required for normal male fertility and is dynamically localized in cytoplasmic particles in Drosophila testis cells. Biochem J. 2008;416(3):327–35. pmid:18652574.
- 61. Gönczy P, Matunis E, DiNardo S. bag-of-marbles and benign gonial cell neoplasm act in the germline to restrict proliferation during Drosophila spermatogenesis. Development. 1997;124(21):4361–71. pmid:9334284.
- 62. Bailey AS, Batista PJ, Gold RS, Chen YG, de Rooij DG, Chang HY, et al. The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline. eLife. 2017;6. Epub 20171031. pmid:29087293; PubMed Central PMCID: PMC5703642.
- 63. Hsu PJ, Zhu Y, Ma H, Guo Y, Shi X, Liu Y, et al. Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis. Cell research. 2017;27(9):1115–27. Epub 20170815. pmid:28809393; PubMed Central PMCID: PMC5587856.
- 64. Wojtas MN, Pandey RR, Mendel M, Homolka D, Sachidanandam R, Pillai RS. Regulation of m(6)A Transcripts by the 3’→5’ RNA Helicase YTHDC2 Is Essential for a Successful Meiotic Program in the Mammalian Germline. Mol Cell. 2017;68(2):374–87.e12. Epub 20171012. pmid:29033321.
- 65. Jain D, Puno MR, Meydan C, Lailler N, Mason CE, Lima CD, et al. ketu mutant mice uncover an essential meiotic function for the ancient RNA helicase YTHDC2. eLife. 2018;7. Epub 20180123. pmid:29360036; PubMed Central PMCID: PMC5832417.
- 66. Liu R, Kasowitz SD, Homolka D, Leu NA, Shaked JT, Ruthel G, et al. YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis. Cell reports. 2021;37(11):110110. pmid:34910909; PubMed Central PMCID: PMC8720241.
- 67. Li L, Krasnykov K, Homolka D, Gos P, Mendel M, Fish RJ, et al. The XRN1-regulated RNA helicase activity of YTHDC2 ensures mouse fertility independently of m(6)A recognition. Mol Cell. 2022;82(9):1678–90.e12. Epub 20220318. pmid:35305312.
- 68. Saito Y, Hawley BR, Puno MR, Sarathy SN, Lima CD, Jaffrey SR, et al. YTHDC2 control of gametogenesis requires helicase activity but not m(6)A binding. Genes Dev. 2022;36(3–4):180–94. Epub 20220120. pmid:35058317; PubMed Central PMCID: PMC8887132.
- 69. Li Y, Minor NT, Park JK, McKearin DM, Maines JZ. Bam and Bgcn antagonize Nanos-dependent germ-line stem cell maintenance. Proc Natl Acad Sci U S A. 2009;106(23):9304–9. Epub 20090522. pmid:19470484; PubMed Central PMCID: PMC2695086.
- 70. Lolicato F, Marino R, Paronetto MP, Pellegrini M, Dolci S, Geremia R, et al. Potential role of Nanos3 in maintaining the undifferentiated spermatogonia population. Dev Biol. 2008;313(2):725–38. Epub 2007/12/20. pmid:18089289.
- 71. Suzuki A, Saga Y. Nanos2 suppresses meiosis and promotes male germ cell differentiation. Genes Dev. 2008;22(4):430–5. Epub 2008/02/19. pmid:18281459; PubMed Central PMCID: PMC2238665.
- 72. Sada A, Suzuki A, Suzuki H, Saga Y. The RNA-binding protein NANOS2 is required to maintain murine spermatogonial stem cells. Science. 2009;325(5946):1394–8. Epub 2009/09/12. pmid:19745153.
- 73. Darnell JC, Van Driesche SJ, Zhang C, Hung KY, Mele A, Fraser CE, et al. FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism. Cell. 2011;146(2):247–61. Epub 2011/07/26. pmid:21784246; PubMed Central PMCID: PMC3232425.
- 74. Greenough WT, Klintsova AY, Irwin SA, Galvez R, Bates KE, Weiler IJ. Synaptic regulation of protein synthesis and the fragile X protein. Proc Natl Acad Sci U S A. 2001;98(13):7101–6. pmid:11416194; PubMed Central PMCID: PMC34629.
- 75. Zalfa F, Achsel T, Bagni C. mRNPs, polysomes or granules: FMRP in neuronal protein synthesis. Curr Opin Neurobiol. 2006;16(3):265–9. Epub 20060516. pmid:16707258.
- 76. Maurin T, Zongaro S, Bardoni B. Fragile X Syndrome: from molecular pathology to therapy. Neurosci Biobehav Rev. 2014;46 Pt 2:242–55. Epub 20140122. pmid:24462888.
- 77. Graf R, Munschauer M, Mastrobuoni G, Mayr F, Heinemann U, Kempa S, et al. Identification of LIN28B-bound mRNAs reveals features of target recognition and regulation. RNA Biol. 2013;10(7):1146–59. pmid:23770886; PubMed Central PMCID: PMC3849162.
- 78. Hafner M, Max KE, Bandaru P, Morozov P, Gerstberger S, Brown M, et al. Identification of mRNAs bound and regulated by human LIN28 proteins and molecular requirements for RNA recognition. RNA. 2013;19(5):613–26. pmid:23481595; PubMed Central PMCID: PMC3677277.
- 79. Wilbert ML, Huelga SC, Kapeli K, Stark TJ, Liang TY, Chen SX, et al. LIN28 binds messenger RNAs at GGAGA motifs and regulates splicing factor abundance. Mol Cell. 2012;48(2):195–206. pmid:22959275; PubMed Central PMCID: PMC3483422.
- 80. Chen D, Wu C, Zhao S, Geng Q, Gao Y, Li X, et al. Three RNA binding proteins form a complex to promote differentiation of germline stem cell lineage in Drosophila. PLoS Genet. 2014;10(11):e1004797. Epub 20141120. pmid:25412508; PubMed Central PMCID: PMC4238977.
- 81. Dai X, Cheng X, Huang J, Gao Y, Wang D, Feng Z, et al. Rbm46, a novel germ cell-specific factor, modulates meiotic progression and spermatogenesis. Biology of reproduction. 2021;104(5):1139–53. pmid:33524105.
- 82. Wang C, Chen Y, Deng H, Gao S, Li L. Rbm46 regulates trophectoderm differentiation by stabilizing Cdx2 mRNA in early mouse embryos. Stem cells and development. 2015;24(7):904–15. pmid:25397698.
- 83. Zhai L, Wang C, Chen Y, Zhou S, Li L. Rbm46 regulates mouse embryonic stem cell differentiation by targeting beta-Catenin mRNA for degradation. Plos One. 2017;12(2):e0172420. pmid:28212427; PubMed Central PMCID: PMC5315375.
- 84. Retraction: Rbm46 regulates mouse embryonic stem cell differentiation by targeting β-Catenin mRNA for degradation. PLoS One. 2021;16(11):e0259919. Epub 20211109. pmid:34752488; PubMed Central PMCID: PMC8577737.
- 85. Niedenberger BA, Geyer CB. Advanced immunostaining approaches to study early male germ cell development. Stem cell research. 2018;27:162–8. Epub 2018/02/25. pmid:29475796; PubMed Central PMCID: PMC5894494.