As the central dogma of molecular biology, genetic information flows from DNA through transcription into RNA followed by translation of the message into protein by transfer RNAs (tRNAs). However, mRNA translation is not always perfect, and errors in the amino acid composition may occur. Mistranslation is generally well tolerated, but once it reaches superphysiological levels, it can give rise to a plethora of diseases. The key causes of mistranslation are errors in translational decoding of the codons in mRNA. Such errors mainly derive from tRNA misdecoding and misacylation, especially when certain codon-paired tRNA species are missing. Substantial progress has recently been made with respect to the mechanistic basis of erroneous mRNA decoding as well as the resulting consequences for physiology and pathology. Here, we aim to review this progress with emphasis on viral evolution and cancer development.
Citation: Ou X, Cao J, Cheng A, Peppelenbosch MP, Pan Q (2019) Errors in translational decoding: tRNA wobbling or misincorporation? PLoS Genet 15(3): e1008017. https://doi.org/10.1371/journal.pgen.1008017
Editor: Ronald B. Gartenhaus, University of Maryland Medical School, UNITED STATES
Published: March 28, 2019
Copyright: © 2019 Ou et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by grants from the National Key Research and Development Program of China (2017YFD0500800), China Agricultural Research System (CARS-42-17), Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System (CARS-SVDIP) and Special Fund for Key Laboratory of Animal Disease and Human Health of Sichuan Province (2016JPT0004). QP is supported by the KWF (Dutch Cancer Society) Young Investigator (10140). XO is supported by the China Scholarship Council for Joint-Ph.D. fellowships (No. 201706910003). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
In all living organisms, DNA is transcribed into RNA, and RNA is translated into protein. The latter process is executed by the ribosome, which constitutes the translation machinery that produces the cellular proteome by decoding mRNAs. Deciphering mRNA codons by transfer RNAs (tRNAs) in the ribosome involves Watson-Crick base pairing . However, the translation machinery is not always perfect, and errors in the amino acid composition may occur [2–5]. The general error rates of genomic replication (about 10−8) are estimated to be approximately 10,000-fold lower than those of protein synthesis (about 10−4), and thus in most instances mRNA translation is the key process contributing to inaccuracy of the cellular proteome . The discrepancy between error rates in DNA replication and mRNA translation may partially relate to the fact that DNA replication occurs at the level of individual nucleotides (involving 41 = 4 possible permutations), whereas the translation machinery interprets mRNA codons in triplets (involving 43 = 64 possible permutations) .
In the canonical interpretation, 61 aminoacyl-tRNAs and 3 suppress tRNAs decode 64 triplet codons that specify 20 amino acids . The resulting redundancies in the genetic code attribute to synonymous codons, which involve wobbling at position 3. For each amino acid, the number of codon usage varies from two to six according to codon degeneracy. In parallel, the numbers of certain amino acid–specified tRNAs (based on recognition of anticodons) also vary from two to six box tRNA sets. Translational decoding of the mRNA codons is constrained by factors during codon–anticodon recognition and often constitutes the rate-limiting step during protein synthesis. Besides the abundance of tRNA species, mRNA translation is regulated by nearly 100 epigenetic tRNA modifications, especially at the wobble position [8, 9]. The efficiency of mRNA decoding machinery is also essentially regulated by codon usage bias that is distinguished by over- or underrepresented synonymous codons [10, 11]. Accordingly, optimizing of tRNA wobble and codon usage in mRNA can substantially enhance translation efficiency and accuracy [10–12].
Nevertheless, mistranslation universally occurs. Pre- or post-mRNA translation may indirectly introduce errors of protein synthesis during transcription and posttranslational processing . However, the translation machinery can directly contribute to mistranslation by tRNA misdecoding (leading to misincorporation or stop-codon readthrough), tRNA misacylation (leading to wrong tRNA–amino acid coupling), codon reassignment or ribosomal translocation-provoked frameshifts (Fig 1) . It is becoming increasingly clear that such mistranslation has consequences on the pathophysiology of a variety of diseases (Fig 1) (Table 1), including multiple sclerosis, neurodegeneration, mitochondrial myopathy, encephalopathy, lactic acidosis, stroke-like episodes, Parkinson's disease, and cancer [14–19]. In this review, we aim to describe the key mechanisms that underlie mistranslation and illustrate potential implications using viral evolution and carcinogenesis as examples.
In physiological conditions, errors in mRNA translation may occur but are generally well tolerated. However, the frequency of errors is dramatically increased in response to stresses. When amino acid misincorporation reaches intolerable levels, this contributes to dysfunction of cellular physiology and may cause pathogenesis. In general, the error ratio in translation decoding primarily depends on tRNA wobbling (cognate) and misdecoding (noncognate) as well as misacylation of tRNAs. aa, amino acid; iMet-tRNA, initiator tRNA Methionine; tRNA, transfer RNA.
tRNA wobbling compensates for missing tRNA species
In the ribosome, tRNAs detect appropriate mRNA codons using the anticodon loop and transfer proper amino acids to polypeptides. However, the number of obligatory tRNA species (based on anticodons) for mRNA translation is substantially smaller than the theoretically required 64 species necessary for full codon matching . Life solves this problem by allowing wobbling or superwobbling (also known as the “four-way wobbling”), thus allowing fewer tRNA species to translate all mRNA codons (Table 1) [20–22]. In the human genome, there are approximately 10-fold excess of tRNA gene copies as compared to the number of possible codons (613 versus 64) [23, 24]. Nevertheless, the recently released GtRNAdb 2.0 database indicates that 15 out of theoretically necessary 64 tRNA species are missing, partially because of low confidence (scores < 50), including eight tRNAA34NN and seven tRNAG34NN (Fig 2) .
In the central dogma, DNA transcribes RNA and RNA translates protein. In the human mutation database, the major (57%) mutation types are missense/nonsense (the right panel) that reflect the consequence of DNA errors at genomic level. Ribosome, as the translation machinery, essentially transduces genetic code to functional protein performed by aa-tRNAs. In human genome, 15 out of 64 tRNA types are actually missing partially because of low confidence (score < 50), including eight tRNAANN and seven tRNAGNN. Because of these missing tRNAs and the expanding wobble rules, the mRNA codon can be decoded by cognate or noncognate tRNAs, leading to modulation of translation efficiency and misincorporation (the left panel). At the bottom, the revised wobble rules and the consequent wobble types are listed according to the wobble position 3 of triplet codon. As for eight tRNAANN, NNU codons will be decoded by NNG or NNU anticodon of tRNAs. As for seven tRNAGNN, NNC codons will be decoded by NNA(I) or NNU anticodon of tRNAs. For specific missing tRNAs, the consequent wobble (tRNA wobbling or misdecoding) are detailed in Fig 3. aa-tRNA, aminoacyl-tRNA; tRNA, transfer RNA.
How to decode these codons without fully paired tRNAs remains an intriguing question. Because of wobbling and superwobbling, it is possible to use 32 tRNA species for decoding all 64 possible codons [1, 22]. In plastid genomes, even 25 tRNA species suffice protein biosynthesis by “four-way wobbling” . tRNA species with an unmodified U at wobble site can decode all four triplets (NN/A, G, C, and U). This relaxed wobble has been identified in Mycoplasma spp. and particular organelles, including mitochondria and, as mentioned, in plastids [21, 25, 26]. Therefore, to decode those unpaired codons, cognate or noncognate tRNAs are forced to wobble at position 3 of the codons by wobbling or superwobbling (Fig 3).
Sixty-four tRNA sets are summarized and specified in parallel with codon degeneracy (left bottom and central). Faithful or misincorporated protein can result from decoding by cognate or near-cognate tRNA at position 3. For eight missing tRNAANN (yellow text), NNU•tRNAUNN wobble-dependent misdecoding by near-cognate tRNAs mainly occurs at the two box (I–VI) and six box tRNA sets (arginine-serine misincorporation) (VII). For seven missing tRNAGNN (red text), NNC codons will be decoded by cognate tRNAs without amino acid misincorporation (VIII) because they happen at the four and six box tRNA sets. Since leucine and phenylalanine share UUN codon, leucine-phenylalanine misincorporation may occur across the six and two box tRNA sets. Besides wobbling at position 3, mRNA codons can be falsely decoded by “far-cognate” tRNA at position 1 and 2 (in the text). Missing tRNAs are indicated as question mark. Individual wobble and misdecoding are labeled as green and red text, respectively. tRNA, transfer RNA.
Decoding unpaired codons by excessive tRNA wobbling
Though tRNA wobbling enables translation compatibility, this also increases the probability of misdecoding by noncognate tRNAs. Among the eight missing species of tRNAANN, the NNU codons likely only pair with tRNAGNN, tRNAUNN, and tRNAINN as dictated by the revised wobble rules (Table 2) (Fig 2), because the tRNAANN is missing, and so does tRNAINN (leading to wobbling with either adenine, cytosine, or uridine), as conversion of tRNAANN to tRNAINN is catalyzed by the tRNA-dependent adenosine deaminases 2 (ADAT2) . Specifically, if NNU codons pair with tRNAGNN, it will lead to a G•U wobble pair without concomitant amino acid misincorporation, as the same amino acid is coded by NNU and NNC (tRNAGNN) codons (Fig 3). If NNU codons pair with tRNAUNN, however, the resultant U•U pair will cause amino acid misincorporation. NNU and NNA (tRNAUNN) code for different amino acids at the 2-fold degenerate codon box, resulting in leucine→phenylalanine, lysine→asparagine, glutamic acid→aspartic acid, glutamine→histidine, Stop→tyrosine, and selenocysteine→cysteine misdecoding (Fig 3). According to the original wobble hypothesis of Francis Crick, the codons decoded by the two box tRNA sets must distinguish either NNU/C or NNA/G . However, based on the revised wobble rules, NNU•tRNAUNN-mediated decoding is at bay with Crick’s assumption and might lead to misincorporation of amino acids. Leucine→phenylalanine, lysine→asparagine, and glutamine→histidine misincorporations have been reported to occur in bacterial and mammalian cells when such cells suffer from phenylalanine, asparagine, and histidine starvation, respectively [28–30]. Misreading of codons by the “Two-out-of-three” hypothesis, which entails that the first two nucleotides in each codon are essential for anticodon recognition, has been suggested to pose a threat to translation fidelity . This type of misreading may occur in those 2-fold degenerate codons as uniquely discriminated by wobble bases. It has been experimentally proven that tRNA superwobbling suffices to decode all four triplets of 4-fold degenerate codons in plastids [21, 22]. Such superwobbling may allow the 2-fold degenerate codons to cross-decode by NNU•tRNAUNN-mediated decoding (Fig 3).
With respect to the seven missing species of tRNAGNN, the NNC codons are expected to pair with tRNAANN, tRNAUNN, and tRNAINN (Fig 2) (Table 2). All missing tRNAGNN can be decoded through wobble pairing without accompanying misincorporation of amino acids (Fig 3) because they occur in 4- or 6-fold degenerate tRNA boxes for which cognate tRNAs are available (Fig 3). However, misincorporation of amino acid may occur if NNC codons are misdecoded by noncognate tRNAUNN, in which the amount of tRNAGNN is not limiting, because NNC and NNA (tRNAUNN) code for different amino acids in twice-degenerated codons (Fig 3). Furthermore, codon UAU (tyrosine)-UAA (Stop) mismatch will truncate the elongation process of nascent peptide (Fig 3). Conversely, if the stop codon (UGA) is mismatched by a Selcys-tRNAUCA, this will lead to an excessively translation elongation (Fig 3). Arginine and serine share an AGN wobble (AGA and AGG for arginine; AGU and AGC for serine), and this predisposes organisms to a potential arginine→serine misincorporation (Fig 3). Such arginine→serine misincorporation affects the quality of therapeutic antibody production by Chinese hamster ovary cells, illustrating the relevance of mRNA misdecoding . In conclusion, unpaired codons are likely to be misdecoded by noncognate tRNAs because of excessive tRNA wobbling, raising questions as to the consequences of such misdecoding for living organisms .
tRNA wobbling at three codon positions compromises the fidelity of the translation decoder
Nonsense translation, so-called stop-codon readthrough, can result from aberrant decoding of stop codons by noncognate aminoacyl-tRNAs (examples are Gln[CAG/CAA], Tyr[UAU/UAC], and Lys[AAG/AAA] for the UAA and UAG stop codons respectively; Trp[UGG], Arg[AGA], and Cys[UGU/UGC] for the UGA stop codon) [2, 33]. The occurrence of such readthrough highlights the possibility of position 3 and 1 wobbling in translational machinery and provides an indication as to how common translational misdecoding in living organisms is. In ciliates, ribosome profiling has demonstrated that all three stop codons can be misdecoded, whereas rates of such miscoding depend on the position within mRNA molecule (coding region or the end) . Position 1 wobbling occurs not only in stop codons but also in sense codons, such as the misreading of arginine CGU/CGC codons as cysteine UGU/UGC codons [35, 36]. By using the prokaryote ortholog of elongation factor Tu (EF-Tu) for targeted mass spectrometry, it has been reported that even position 2 can be misdecoded by noncognate tRNAs, as illustrated by the detection of the arginine CGU codon misdecoded by tRNAGAG-Leu . Thus, substantial misdecoding at all three positions is possible [2, 5] (Table 1). This consequently compromises the fidelity of the translation decoder.
It has been reported that G•T mismatching occurs in both DNA and RNA duplex following tautomerization and ionization, and this plays important roles in replication and translation errors [37, 38]. The Watson-Crick-like mismatch can evade fidelity checkpoints and appears to occur with probabilities (10−3 to 10−5) that strongly imply a universal role of this mismatch in translation errors . The rG•rT mismatch at position 3 may not lead to mistranslation in decoding center, because NNU and NNC (rG•rC/rU) code for the same amino acids in such twice-degenerated codons, and the same holds true for NNG and NNA (rU•rA/rG) (Fig 3). However, more mistranslation results if rG•rT mismatch takes place at position 1 and 2 [5, 35, 36]. Hence, in toto a picture emerges—that amino acid misincorporation in the nascent peptide chain is prone to occur mainly because of the absence of fully Watson-Crick pairing tRNAs and by excessive wobbling at all three codon positions .
Quality control of the translation machinery
Faithful translation of the mRNA codons into protein is essential for cellular physiology. The fidelity of the translation machinery firstly depends on the specific coupling of amino acids to their cognate tRNA species, which is catalyzed by aminoacyl-tRNA synthetases (aaRSs) (Fig 4a and 4b). aaRS is capable of discriminating its cognate substrates from structurally analogous tRNAs and amino acids . Subsequently, eukaryotic elongation factor 1A (eEF-1A) or prokaryotic EF-Tu delivers the aminoacyl-tRNA to the ribosome A site for elongation of nascent peptide chain after proper codon–anticodon recognition . Thus, aaRSs are cardinal in protecting protein synthesis against misacylation , but their specificity is not absolute. For instance, in E. coli, four types of misacylated-tRNA—including Cys-tRNAPro, Ser-tRNAThr, Glu-tRNAGln, and Asp-tRNAAsn—do not evoke a correctional reaction . In both mice and bacteria, serine is prone to be misacylated by alanyl-tRNA synthetases (AlaRSs) . In mycobacteria, an increase in the substitution of glutamic acid→glutamine and aspartic acid→asparagine by translational misincorporation has been linked to phenotypic resistance to rifampicin treatment . Thus, beneficial mistranslation in both prokaryotes and eukaryotes may exist and improve their survival or facilitate drug resistance [43–45]. Apart from misdecoding, misacylation of amino acids to tRNA molecules is another important source of mistranslated proteins, despite the presence of mechanisms preventing such events.
(a) aa-tRNAs are synthesized by sampling from the amino acid pool and tRNA pool and require catalysis by aaRSs. This process may accidently introduce misacylated aa-tRNAs, because the types of tRNAs and amino acids are difficult to be distinguished by involved aminoacyl synthetase because of analogous structures. (b) During elongation, tRNA wobbling will increase translation efficiency. Misincorporation can also be introduced because of tRNA misdecoding (amino acid misincorporation caused by excessive wobble decoding), especially when certain codon-paired tRNA species are missing. Finally, the fidelity of translation machinery will be impaired and produce mutated proteome, including RNA and DNA polymerases, aaRSs, and accessories. (c) Mistranslation of RdRP in RNA viruses will augment generation of a mutated virome (quasispecies) and facilitate viral evolution and adaption. (d) Similarly, mistranslation of cellular DNA replication-related enzymes and relative proteins amplifies mutagenesis in the genome and contributes to cancer development. aaRS, aminoacyl-tRNA synthetase; aa-tRNA, aminoacyl-tRNA; RdRP, RNA-dependent RNA polymerase; tRNA, transfer RNA.
How could tRNA wobbling guarantee faithful decoding by the codon–anticodon duplex? During elongation, eEF-1A or EF-Tu delivers amino acid–coupled tRNA to the ribosome A site . Subsequently, the ribosome rechecks the codon–anticodon duplex that involves the highly conserved G530, A1492, and A1493 of 16S RNA via stabilization of the first two Watson-Crick pairs of the duplex [31, 46]. A correct confirmation of the codon–anticodon duplex will induce a conformational domain closure in the ribosome and result in the formation of the appropriate peptide bond and elongate the nascent protein . Analysis of X-ray structures suggests that the positions 1 and 2 of the A codon are obligatory Watson-Crick base pairs. In prokaryotes, when U•G and G•U wobbles at the first or second codon–anticodon position, the decoding center forces this pair to adopt the geometry close to that of a canonical C•G pair . Using nuclear magnetic resonance (NMR) relaxation dispersion, it has recently been revealed that dG•dT misincorporation during replication is likely mediated via tautomerization and ionization . As discussed, these Watson-Crick-like mismatches may further contribute to tRNA wobbling and consequently misdecoding . Although the hydrogen bond is the major force to form codon–anticodon pairs , the van der Waals forces, steric complementarity, and shape acceptance may concurrently contribute to the codon–anticodon recognition essentially for quality control [3, 40].
mRNA mistranslation in physiology
The integrity of mRNA translation sustains essential cellular physiology in all domains of life. Low level of mistranslation, however, is well tolerated and even contributes to stress responses, as it creates a degree of diversity in the proteome (also known as “statistical proteome”) . Yeasts engineered to misincorporate serine at leucine CUG codon initially lose fitness but quickly adapt by promoting the evolution of genome architecture . Experiments employing misacylated aminoacyl-tRNAs show that up to 10% of overall mistranslation in E. coli does not compromise physiology of this organism and is even compatible with bacterial proliferation . aaRSs of mycoplasma with mutations in the editing domain provoke misacylation tRNAs with highly similar amino acids that contribute to antigen diversity as to escape host immune defenses . In mammalian cells, up to 10-fold methionyl-misacylation to non–methionine-tRNAs will protect against reactive oxygen species (ROS)-mediated damage when cells undergo oxidative stress, such as exposure to viral infections, Toll-like receptor ligands, or xenobiotics .
Rates of mistranslation vary dramatically between organisms and different environmental conditions (Table 1). An overall amino acid misincorporation rate of approximately 3‰–5‰ during translation is regarded as compatible with normal physiology [50, 51]. In contrast, exceeding 1% misincorporation is usually deleterious and may provoke pathogenesis . For example, the 50% tRNAAla mischarging with serine residues by an editing-defective AlaRS is associated with neurodegeneration . In addition, defective AlaRS is also related to cardioproteinopathy . However, the capacity of organisms to deal with mistranslation appears diverse, and subphysiological mistranslation is tolerant and even beneficial.
Errors of translation and viral evolution
Viral genomes are dynamically mutated with frequent emergence of new quasispecies. The spectrum for the hypermutation of viral genomes, sometimes denominate as mutant clouds . Mutation rates at genomic level (substitutions per nucleotide per cell infection [s/n/c]) range from 10−8 to 10−6 s/n/c for DNA viruses and from 10−6 to 10−4 s/n/c for RNA viruses . Apparently, there is an error threshold to constrain viral evolution dependent on the genome size and permutations of errors . Within the virome, RNA viruses in particular mutate tremendously as a consequence of RNA-dependent RNA polymerases (RdRPs) being error-prone. For instance, the mutation rate of RdRPs that mediate poliovirus and foot-and-mouth disease virus (FMDV) replication can further expand or reduce the quasispecies diversity by regulation of replication fidelity [56, 57]. The consequences of the mutations highly depend on both the position and properties of the affected amino acid residues. To take FMDV as an example, a W237F mutation but not a W237I mutation in the polymerase leads to a high fidelity and thus contributes to the subsequent mutation rates .
Little is known of the consequences of an error-prone translation machinery on viral evolution. As discussed, erroneous protein synthesis is prone to occur especially when cells suffer cellular stresses like viral infection. In this situation, the viral RNA is possibly mistranslated during the inaccurate translation . Several types of errors in the translational machinery have been linked to viral adaptability. An example is the apparent selective pressure exerted on fungal mitovirus to exclude UGA (tryptophan) codons from its coding sequence because of the lack of fidelity of decoding this codon by the host mitochondrion . When organisms are recoded to obtain nonassigned codons, compensatory mechanisms emerge, including frameshifts and stop-codon readthrough . In yeast, mistranslation has been demonstrated to provoke evolution of genomic architecture . Hence, genomic mutagenesis is substantially associated with mistranslation that promotes the likelihood of evolution . It is well possible that viruses also utilize mistranslated genome-copying machinery (e.g., RdRP) for viral evolution. Besides the classical mutations inherited from error-prone replication at the genomic level, we propose that mistranslation may generate additional RdRP mutants at the protein level that are not inheritable. Except for negative-strand RNA viruses, there is no RdRP incorporated in the virion. Therefore, upon infection, translation of the viral genome is invariably ahead of its replication. Thus, when an RNA virus releases its genome into the host cell after uncoating, errors in RdRP may be accidently introduced by mistranslation [58, 59]. The resulting mixture of wild-type and mutated RdRP enzymes initiate replication associated with a spectrum of viral quasispecies (Fig 4c). Those species that possess the best viral fitness finally survive and become dominant.
As for DNA viruses, the mechanisms driving viral mutation are more diverse and less well understood. Degradation of HIV-1 proviral DNA with G→A hypermutation has an important role in host responses to infection . This sublethal mutagenesis catalyzed by cytidine deaminases in the family of apolipoprotein B RNA-editing catalytic polypeptide-like 3 (APOBEC3) can induce drug-resistant and generate immune-escape viruses [60, 61]. In hepatitis B virus–infected patients, however, such mutations may have undesired consequences with respect to the viral reverse transcriptase (e.g., the A181T and M204I mutations) and mediate adefovir resistance . Analogously, it has been reported that mutations in palm, finger, and 3′-5′ exonuclease domains of herpesviruses DNA polymerase are introduced as a consequence of nucleoside analogue-based therapy . Mistranslation in DNA viruses can also generate viral proteins that are more prone to provoke mutations in the viral genome, but hard data for this notion are currently still lacking.
Errors of translation in cancer development
Malignant transformation is usually associated with accumulation of large numbers of DNA mutations. Once occurring in essential oncogenes and tumor suppressors, these are also intimately associated with cancer development and progression [64, 65]. The importance of DNA mutation-dependent alteration in protein composition is illustrated by the recent identification of approximately 3,400 driver mutations in tumor exomes . In the human mutation database, 57% of the mutations are missense/nonsense (Fig 2). This reflects the major consequences of DNA errors that are driven by either DNA replication errors or environmental factors. Apart from genomic alterations, mistranslation may also be important in cancer cells. It has been reported that DNA replication errors are responsible for two-thirds of the mutations observed in 17 cancer types . Hence, reduced fidelity of DNA-replicating enzymes appears more important than environmental factors for generating cancer-associated mutations. The implication of this notion is that if mistranslation of DNA-replicating enzymes reduces replication fidelity, this would be expected to further advance cancer development . Of note, translation machinery is largely rewired during tumorigenesis . By shaping tRNA pool to match protumorigenic mRNAs, the translation of oncogenes is facilitated to prime oncogenesis, such as highly up-regulated tRNAGlu-UUC and tRNAArg-CCG in breast cancer [70, 71]. Moreover, mutated components of ribosome are involved in carcinogenesis as well and may foster disease by compromising the ribosome (translation fidelity) to “translate” cancer . For example, missense mutations of the ribosomal protein RPS15, a component of the 40S ribosomal subunit, is involved in chronic lymphocytic leukemia . How the compromised translation machinery contributes to the nature of hypermutated tumor transformation at the genomic level is an intriguing question. In analogy to RNA viruses, mistranslation of DNA polymerases and APOBEC3H in cancer may occur before genomic replication [68, 73–75]. The cellular proteome in G1 phase of cell cycle must duplicate before S phase, and the demand on the translational machinery may provoke errors with respect to mRNA decoding . The ribosomal fidelity in (pre-) malignant cells may become compromised, resulting in mistranslated DNA polymerase molecules, which in turn drive further genomic instability [69, 75]. This further contributes to hypermutation and consequently tumorigenesis (Fig 4d) [70, 77]. In apparent support of this notion, mistranslation caused by serine-to-alanine misreading tRNA has been shown to promote the development of epithelial cancer in mouse models . Moreover, mutated DNA polymerase ε (P286R) in mice models provokes ultra-mutagenesis that can rapidly develop into lethal cancers of diverse lineages .
It is important to note that N→T missense mutations are widespread in cancer . This type of mutation increases translation efficiency through facilitating tRNA wobbling and superwobbling that provides the cancer cells with advantage to compete clones but will concomitantly provoke amino acids misincorporation, especially when the two box tRNA sets are involved (Fig 3). As described, epigenetic modification of tRNA (U34) further supports tumorigenesis by up-regulating U34 enzymes and enhancing codon wobble of especially tumor promoting genes, an effect that prominently involves SRY-box 9 (SOX9) and elongator complex protein 3 (Elp3) [78, 79]. A high level of the U34 enzyme promotes alternative translation and has been linked to resistance to anti-BRAF therapy through wobble decoding of hypoxia-inducible factor 1A (HIF1A) mRNA in a codon-specific manner . Thus, the error-prone translation machinery appears to contribute to mutagenesis during cancer development.
Though mistranslation promotes carcinogenesis, it also offers possible targets for anticancer therapeutics. Targeting enzymes catalyzing U34 tRNA modification has been demonstrated the potential for treating melanoma . Depletion of the U34 enzymes Elp3 or cytoplasmic tRNA 2-thiolation protein 1/2 (CTU1/2) provokes cell death in patient-derived BRAFV600E melanoma cultures . Genetic incorporation of noncanonical amino acids by decoding specific a codon is another approach . Misincorporations of p-acetylphenylalanine at target codons have been explored to develop bispecific antibody-based therapy for breast cancer and acute myeloid leukemia [83, 84]. Moreover, certain mutant peptides of human tumors can serve as T-cell epitopes for immunotherapy . These tumor-specific immunogens as potentially personalized vaccines have been shown to boost immune rejection to the tumors in mouse model [85, 86].
Conclusion and perspective
mRNA mistranslation universally occurs across all living organisms. It is generally well tolerated in physiology and even helps the organism adapt and withstand cell stresses. However, excessive mistranslation is pathogenic and implicated in many diseases. Mistranslation may also provide targets for drug and vaccine development, in particular against viral infection and cancer.
Although mRNA mistranslation can be caused by a variety of mechanisms, tRNA misdecoding and tRNA misacylation are the key drivers. The former is largely attributed to the partially missing tRNAs and excessive wobbling decoding. Consequently, mRNA codons can be coupled to cognate or near-cognate tRNAs at position 3, leading to modulation of translation efficiency and misincorporation . By furthering wobbling at position 1 and 2, mRNA codon can be falsely decoded by “far-cognate” tRNAs. We speculate that if wobbling or superwobbling concurrently occurs at all three positions, especially with regard to the codons decoded by the two box tRNA sets, no functional protein would likely be produced.
The development of high-throughput sequencing and ribosome profiling technologies has greatly advanced our understanding of tRNA decoder . However, proteomic analysis at single molecular level remains technically infeasible. This hampers a detailed characterization of the protein “quasispecies” pool that results from mistranslation. In the future, deciphering single codon–anticodon decoding will help providing more mechanistic insights as to how tRNA decoding relates to translation fidelity.
We apologize to colleagues whose work was not cited because of the content limit. We also thank Holsan Tang (EUR Rotterdam) for graphics support and valuable suggestions.
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