Figures
The top left image of Fig 2B is incorrect. The authors have provided a corrected version of Fig 2 here.
(A) Western blot showing similar levels of expression for N- and C-terminal FLAG-tagged wild-type RPS7 (WT-N and WT-C, respectively) and RPS7Mtu (M-N and M-C, respectively) proteins in HEK-293 cells. The RPS7-specific band is indicated by *, and NPT2 (arrowhead) expression is shown as a control. (B) Subcellular localization of N- and C-terminal FLAG-tagged RPS7 proteins. Wild-type RPS7 and RPS7Mtu both localize to speckles in the nucleus and are observed throughout the cytoplasm. Scale bar = 10 μm. All panels are at the same magnification. (C) Representative Northern blot analysis of liver and brain RNA from wild-type (WT) and Rps7Mtu/+ (M) mice detecting various rRNA precursors using a probe within the internal transcribed spacer (ITS1). The 30S and 21S rRNA precursors are indicated. (D) Quantitation of the signals of Northern experiments reported as the ratio between 30S and 21S rRNA precursors was significantly different between Rps7+/+ and Rps7Mtu/+ (* indicates p<0.01). The average of the values is reported in the bar graphs with S.E.M.
Reference
Citation: Watkins-Chow DE, Cooke J, Pidsley R, Edwards A, Slotkin R, Leeds KE, et al. (2015) Correction: Mutation of the Diamond-Blackfan Anemia Gene Rps7 in Mouse Results in Morphological and Neuroanatomical Phenotypes. PLoS Genet 11(11): e1005682. https://doi.org/10.1371/journal.pgen.1005682
Published: November 19, 2015
Copyright: © 2015 Watkins-Chow et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited