Citation: Arnot DE (2014) How Malaria Parasites Avoid Running Out of Ammo. PLoS Genet 10(12): e1004878. https://doi.org/10.1371/journal.pgen.1004878
Editor: Kirk Deitsch, Weill Medical College of Cornell University, United States of America
Published: December 18, 2014
Copyright: © 2014 David E. Arnot. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: The author received no specific funding for this article.
Competing interests: The author declares that no competing interests exist.
Antigenic Variation in Plasmodium falciparum
Malaria parasites operate antigenic variation systems to avoid antibody recognition, thereby inhibiting their host's capacity to clear infections . Plasmodium falciparum, the most pathogenic of the human malaria species, bases immune evasion on switching expression of approximately 60 var genes. These encode transmembrane proteins (PfEMP1 antigens) on the red blood cell surface, which allow intraerythrocytic (IE) parasites to adhere to endothelial molecules . PfEMP1 proteins are thus not simply antigenic decoys, but functional vascular adhesion receptors. The disappearance of older parasites from the bloodstream results from PfEMP1 binding to endothelial receptors, a process called sequestration. This enables replicating stages to avoid the dangerous passage through the spleen, the main organ of defence against blood infection . In this issue, Claessens et al.  present new and intriguing data on how antigenic diversity can be continuously generated during persistent malaria infections.
Linking Antigenic Variation, Sequestration, and Pathology
Sequestration affects the course of clinical malaria ; and recent studies have linked the binding of specific PfEMP1s to endothelial protein C receptor (EPCR) with severe malaria pathology . This extends studies connecting pregnancy malaria with binding of a form of PfEMP1 called VAR2CSA to placental chondroitin sulphate A (CSA) . Individuals who experience multiple infections gradually acquire low level immunity that prevents the severe symptoms of the disease, but that does not prevent infection. One model of malaria pathogenesis proposes that after repeated exposures to parasites, there is progressive acquisition of blocking antibodies to a broad spectrum of PfEMP1 antigens. The most strongly adhesive PfEMP1 variants appear in early infections, since such variants would have greatest advantage in the absence of effective blocking antibodies. Naïve hosts would be most at risk, and the appearance of novel host receptors, for example the distinctive CSA present on the placental endothelial cells, selects for parasite PfEMP1 variants, which first time mothers would not previously have experienced and to which they had no antibodies .
The Repertoire Problem
While the “PfEMP1 binding determines pathology” hypothesis offers explanations for several observations on severe malaria and the age-dependent acquisition of immunity, it is not just the details that remain to be nailed down. The PfEMP1 proteins are encoded by approximately 60 var genes, their extracellular portion encoded by exon 1, a smaller intracellular domain encoded by exon 2. The extracellular domains are highly ordered combinations of 628 “conserved minimal PfEMP1 building blocks” . Practically all PfEMP1 encoding genes are intact and expressed in situ from telomeric and internal sites on 13 of the 14 P. falciparum chromosomes. This is a modest and conservatively ordered assembly compared to the approximately 1,600 strong trypanosome variant surface glycoprotein gene battery, most of which (65%) are pseudogenes on 11 megachromosomes, several intermediate chromosomes, and approximately 100 minichromosomes . How P. falciparum modulates variant switching to avoid “running out of repertoire” during infections is not understood, particularly since switching rates appear high enough to easily run through 60 genes in an infection . It is also notable that P. falciparum avoids creating var pseudogenes with trypanosome-like abandon.
The Whole Genome Sequencing Approach
Understanding the generation of var gene diversity clearly requires closer study of var recombination; and significant advances are reported by Claessens et al. in this issue. Studies of a handful of var crossovers revealed that it is usually ectopic (nonallelic) –. However, such small samples precluded crossover rate estimates and did not definitively establish where and when var recombination occurs. To increase event detection by screening large numbers of genomes, Claessens et al.  established cultures of P. falciparum isolates prior to cloning by limit dilution and re-expansion from single infected red blood cells. As numerous clonal lineages were generated, mutations arising in mitotically replicating cultures could be detected by whole genome sequencing (WGS). Remarkably, Claessens et al. have now sequenced over 200 P. falciparum clone genomes.
Analysis of 37 subclones of the 3D7 parent clone revealed 20 newly arising single nucleotide polymorphisms (SNPs) and 40 de novo structural genome changes—ten duplications, eight deletions, and 22 translocations. Strikingly, of the 19 structural changes that affected 3D7 exons, all recombined var genes. Other isolate analyses are less comprehensive, but WGS of other similarly generated clonal populations detected 11, 13, and zero var exon 1 recombinations in the Dd2, W2, and HB3 isolates, respectively. The WGS confirms earlier estimates of Bopp et al.  that the SNP mutation rates appear relatively constant between isolates (approximately 9×10−3 per replication cycle), and that genome rearrangements are highly concentrated in regions containing var genes. The var recombination to SNP ratio was calculated to be 0.25, 0.35, 0.54, and zero for 3D7, Dd2, W2, and HB3 respectively, the rate at which var genes recombine estimated to be 2×10−3 per replication cycle. In each 48 hour replication cycle, around 0.2% of parasites could contain a newly recombined var gene. Millions of new var genes will be created with every 48 hour asexual IE replication cycle, presumably when different var genes are in close proximity  during the mitotic chromosome divisions (Fig. 1).
The first mitotic division of single IE P. falciparum genomes occurs from around hours 20–28 of postmerozoite invasion (PI). The three rows illustrate different staining methods used to visualise parasites, nuclei, and chromosomes before, during, and immediately after the first of the parasite's asynchronous mitotic divisions . Giemsa: the developing trophozoite visualised after methanol fixation and Giemsa staining. CPO: Fluorescent staining following Coriphosphine O (CPO) uptake by parasitized erythrocytes. CPO, an acidic acridine derivative, is membrane permeable but shows no fluorescence when free in red blood cells and gives strong green emission using the argon laser (488 nm) when bound to parasite DNA in intact IE. FISH Differential Interference Contrast (DIC): Fluorescence in situ hybridisation (FISH) using cy3-labelled P. falciparum Rep 20 sequences, plus DAPI fluorescence, superimposed on the differential interference contrast image of intact IE parasites. Host cell membranes and vacuolar haemozoin are apparent. Rep 20 hybridises to repetitive sequences at both telomeres of each of the 14 chromosomes. Telomere clustering is evident before, during, and after the nuclear division. It is noteworthy that var genes are often found in tandem arrays immediately adjacent to the telomere repeats, thus telomere clustering could potentially align them for more efficient recombination. The most likely mitotic recombination scenario would appear to be that both DNA secondary structure-induced double strand breaks and microhomology mediated var recombination-associated repair occur during the DNA replication process itself.
WGS yielded an abundant harvest of over 100 crossovers to analyse. These new events conform to the pattern of highly structured ectopic recombination recently reported by Sander et al. . Participating var genes are in the same orientation, often with multiple recombination breakpoints, yet generating functional recombinants by in-frame joining of sequences of the same domain type. Intrinsically recombinogenic DNA secondary structures (DSS) were shown to occur around sites with 15–20 base pairs of near-identical sequence shared between recombination partners. Claessens et al.  confirm that these var-specific microhomology sharing recombinogenic sequences are ubiquitous throughout their much larger sample of crossovers. An analogy can be drawn with variable, diverse, and joining gene segment fusion (V[D]J)-based generation of diversity via recombination in the genes encoding immunoglobulins, although a similar pathway of error-prone nonhomologous end joining is not thought to be present in P. falciparum. Given the frame-conserving nature of var recombination, it seems most likely that accurate, microhomology-based homologous recombination repairs the double strand breaks occurring at var genes, within the DSS-marked recombination hotspots.
Finishing off Malaria?
This remarkable WGS effort on hundreds of clones derived from asexually-generated lineages provides compelling evidence that var gene recombination is mainly, but not exclusively, mitotic in origin. Sex must still advantageously reshuffle var repertoires. The obvious biological advantage to the malaria parasite of high frequency mitotic var recombination, which Claessens et al.  show occurs in no other P. falciparum gene family, is that it allows generation of novel and functional PfEMP1 protein antigens during the malaria infection itself. This is now testable in vivo. The key role of var-based antigenic variation in malaria pathology is further established. We now know that chronic malaria infections survive their encounters with the host immune system  while generating novel antigenic variants via mitotic recombination at a very high rate. A somewhat sobering perspective on the huge problem of eradicating chronic, but asymptomatic, malaria infections is added.
- 1. Brown KN (1973) Antibody induced variation in malaria parasites. Nature 242: 49–50.
- 2. Su X, Heatwole VM, Wertheimer SP, Guinet F, Herrfeldt JA, et al. (1995) The large diverse gene family var encodes proteins involved in cytoadherence and antigenic variation of Plasmodium falciparum-infected erythrocytes. Cell 82: 89–100.
- 3. Baruch DI, Gormley JA, Ma C, Howard RJ, Pasloske BL (1996) Plasmodium falciparum erythrocyte membrane protein 1 is a parasitized erythrocyte receptor for adherence to CD36, thrombospondin, and intercellular adhesion molecule 1. Proc Natl Acad Sci U S A 93: 3497–3502.
- 4. Claessens A, Hamilton WL, Kekre M, Otto TD, Faizullabhoy A, et al. (2014) Generation of Antigenic Diversity in Plasmodium falciparum by Structured Rearrangement of Var Genes During Mitosis. PLoS Genet 10: e1004812.
- 5. Newbold CI, Craig AG, Kyes S, Berendt AR, Snow RW, et al. (1997) PfEMP1, polymorphism and pathogenesis. Ann Trop Med Parasitol 91: 551–557.
- 6. Turner L, Lavstsen T, Berger SS, Wang CW, Petersen JE, et al. (2013) Severe malaria is associated with parasite binding to endothelial protein C receptor. Nature 498: 502–505.
- 7. Dahlback M, Jorgensen LM, Nielsen MA, Clausen TM, Ditlev SB, et al. (2011) The chondroitin sulfate A-binding site of the VAR2CSA protein involves multiple N-terminal domains. J Biol Chem 286: 15908–15917.
- 8. Ricke CH, Staalsoe T, Koram K, Akanmori BD, Riley EM, et al. (2000) Plasma antibodies from malaria-exposed pregnant women recognize variant surface antigens on Plasmodium falciparum-infected erythrocytes in a parity-dependent manner and block parasite adhesion to chondroitin sulphate A. J Immunol 165: 3309–3316.
- 9. Rask TS, Hansen DA, Theander TG, Gorm PA, Lavstsen T (2010) Plasmodium falciparum erythrocyte membrane protein 1 diversity in seven genomes–divide and conquer. PLoS Comput Biol 6: e1000933.
- 10. Marcello L, Barry JD (2007) Analysis of the VSG gene silent archive in Trypanosoma brucei reveals that mosaic gene expression is prominent in antigenic variation and is favored by archive substructure. Genome Res 17: 1344–1352.
- 11. Horrocks P, Pinches R, Christodoulou Z, Kyes SA, Newbold CI (2004) Variable var transition rates underlie antigenic variation in malaria. Proc Natl Acad Sci U S A 2004 101: 11129–11134.
- 12. Deitsch KW, Del Pinal A, Wellems TE (1999) Intra-cluster recombination and var transcription switches in the antigenic variation of Plasmodium falciparum. Mol Biochem Parasitol 101: 107–116.
- 13. Freitas-Junior LH, Bottius E, Pirrit LA, Deitsch KW, Scheidig C, et al. (2000) Frequent ectopic recombination of virulence factor genes in telomeric chromosome clusters of P. falciparum. Nature 407: 1018–1022.
- 14. Bopp SE, Manary MJ, Bright AT, Johnston GL, Dharia NV, et al. (2013) Mitotic evolution of Plasmodium falciparum shows a stable core genome but recombination in antigen families. PLoS Genet 9: e1003293.
- 15. Sander AF, Lavstsen T, Rask TS, Lisby M, Salanti A, et al. (2014) DNA secondary structures are associated with recombination in major Plasmodium falciparum variable surface antigen gene families. Nucleic Acids Res 42: 2270–2281.
- 16. Duffy MF, Byrne TJ, Carret C, Ivens A, Brown GV (2009) Ectopic recombination of a malaria var gene during mitosis associated with an altered var switch rate. J Mol Biol 389: 453–469.
- 17. Hamad AA, El Hassan IM, El Khalifa AA, Ahmed GI, Abdelrahim SA, et al. (2000) Chronic Plasmodium falciparum infections in an area of low intensity malaria transmission in the Sudan. Parasitology 120(Pt 5): 447–456.
- 18. Arnot DE, Ronander E, Bengtsson DC (2011) The progression of the intra-erythrocytic cell cycle of Plasmodium falciparum and the role of the centriolar plaques in asynchronous mitotic division during schizogony. Int J Parasitol 41: 71–80.