The alignment of the immunoblots in Figure 1A is incorrect. The authors have provided a corrected version here.
Panel a. Wild-type (WT) and variant CFTR proteins were expressed in HEK 293T cells and immunoblotted with anti-CFTR and anti-Aldolase antibodies. Replicate lanes are in small font. Band B, expected size of immature ER core-glycosylated CFTR; band C, mature complex-glycosylated CFTR. Panel b. Whole-cell Cl− currents were measured in WT and variant CFTR-expressing HEK 293T cells, as described in Methods. Panel c. Whole-cell currents of WT-CFTR were measured in HEK 293T cells co-expressed with WNK1 and SPAK using patch pipette contained a low concentration of Cl− (10 mM). A representative trace of reversal potential measurement is shown in the left panel. The permeability ratio PHCO3/PCl was calculated according to the Goldman-Hodgkin-Katz equation. I–V relationships at the indicated points are presented in the accompanying graph. The conductance ratio GHCO3/GCl was calculated by measuring each outward current (i.e., slope between Erev and Erev+25 mV). RMP, resting membrane potential. Panel d. Whole-cell currents of R170H-CFTR were measured in HEK 293T cells using the same protocol shown in panel c. Panel e. A summary of the PHCO3/PCl values obtained from WT-CFTR in the standard state (left) compared to WT-CFTR and the nine CFTRBD variants with WNK1 + SPAK activation (right, underlined). Panel f. A summary of the GHCO3/GCl values in the standard state (left) with WNK1 + SPAK activation (right). Values throughout are means ± SEM. * p<0.05, **p<0.01: difference from WT in cells co-expressed with WNK1 and SPAK.
Citation: The PLOS Genetics Staff (2014) Correction: Mechanisms of CFTR Functional Variants That Impair Regulated Bicarbonate Permeation and Increase Risk for Pancreatitis but Not for Cystic Fibrosis. PLoS Genet 10(10): e1004778. https://doi.org/10.1371/journal.pgen.1004778
Published: October 6, 2014
Copyright: © 2014 The PLOS Genetics Staff. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.