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Correction: Mechanisms of CFTR Functional Variants That Impair Regulated Bicarbonate Permeation and Increase Risk for Pancreatitis but Not for Cystic Fibrosis

  • The PLOS Genetics Staff

Correction: Mechanisms of CFTR Functional Variants That Impair Regulated Bicarbonate Permeation and Increase Risk for Pancreatitis but Not for Cystic Fibrosis

  • The PLOS Genetics Staff
PLOS
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The alignment of the immunoblots in Figure 1A is incorrect. The authors have provided a corrected version here.

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Figure 1. Functional characteristics of the nine CFTRBD variants.

Panel a. Wild-type (WT) and variant CFTR proteins were expressed in HEK 293T cells and immunoblotted with anti-CFTR and anti-Aldolase antibodies. Replicate lanes are in small font. Band B, expected size of immature ER core-glycosylated CFTR; band C, mature complex-glycosylated CFTR. Panel b. Whole-cell Cl currents were measured in WT and variant CFTR-expressing HEK 293T cells, as described in Methods. Panel c. Whole-cell currents of WT-CFTR were measured in HEK 293T cells co-expressed with WNK1 and SPAK using patch pipette contained a low concentration of Cl (10 mM). A representative trace of reversal potential measurement is shown in the left panel. The permeability ratio PHCO3/PCl was calculated according to the Goldman-Hodgkin-Katz equation. I–V relationships at the indicated points are presented in the accompanying graph. The conductance ratio GHCO3/GCl was calculated by measuring each outward current (i.e., slope between Erev and Erev+25 mV). RMP, resting membrane potential. Panel d. Whole-cell currents of R170H-CFTR were measured in HEK 293T cells using the same protocol shown in panel c. Panel e. A summary of the PHCO3/PCl values obtained from WT-CFTR in the standard state (left) compared to WT-CFTR and the nine CFTRBD variants with WNK1 + SPAK activation (right, underlined). Panel f. A summary of the GHCO3/GCl values in the standard state (left) with WNK1 + SPAK activation (right). Values throughout are means ± SEM. * p<0.05, **p<0.01: difference from WT in cells co-expressed with WNK1 and SPAK.

https://doi.org/10.1371/journal.pgen.1004778.g001

Reference

  1. 1. LaRusch J, Jung J, General IJ, Lewis MD, Park HW, et al. (2014) Mechanisms of CFTR Functional Variants That Impair Regulated Bicarbonate Permeation and Increase Risk for Pancreatitis but Not for Cystic Fibrosis. PLoS Genet 10(7): e1004376