The maintenance and reformation of gene expression domains are the basis for the morphogenic processes of multicellular systems. In a leaf primordium of Arabidopsis thaliana, the expression of FILAMENTOUS FLOWER (FIL) and the activity of the microRNA miR165/166 are specific to the abaxial side. This miR165/166 activity restricts the target gene expression to the adaxial side. The adaxial and abaxial specific gene expressions are crucial for the wide expansion of leaf lamina. The FIL-expression and the miR165/166-free domains are almost mutually exclusive, and they have been considered to be maintained during leaf development. However, we found here that the position of the boundary between the two domains gradually shifts from the adaxial side to the abaxial side. The cell lineage analysis revealed that this boundary shifting was associated with a sequential gene expression switch from the FIL-expressing (miR165/166 active) to the miR165/166-free (non-FIL-expressing) states. Our genetic analyses using the enlarged fil expression domain2 (enf2) mutant and chemical treatment experiments revealed that impairment in the plastid (chloroplast) gene expression machinery retards this boundary shifting and inhibits the lamina expansion. Furthermore, these developmental effects caused by the abnormal plastids were not observed in the genomes uncoupled1 (gun1) mutant background. This study characterizes the dynamic nature of the adaxial-abaxial specification process in leaf primordia and reveals that the dynamic process is affected by the GUN1-dependent retrograde signal in response to the failure of plastid gene expression. These findings advance our understanding on the molecular mechanism linking the plastid function to the leaf morphogenic processes.
The efficient photosynthesis in plant leaves depends on the wide planar morphology of lamina with a lot of chloroplasts (plastids). Development of the planar lamina requires the specific expression of several key genes in the foreside (adaxial side) and backside (abaxial side) of the tiny leaf primordium. Such abaxial-specific genes include FIL and miR165/166. In this study, we characterized that the expression of FIL and the activity of miR165/166 are induced in all cells of initiating leaf primordia, and then repressed sequentially from the adaxial cells, thus gradually restricted to the abaxial cells. Furthermore, we found that this restriction of FIL expression and miR165/166 activity is retarded and lamina becomes narrow when the plastid function is inhibited in leaf primordia. Interestingly, such plastid effects on leaf development were not observed when the communication between plastid and nucleus was inhibited by gun1 mutation. Our study suggests that plastids modulate the gene expression dynamics in leaf primordia leading to narrow lamina formation when the plastid function is severely inhibited. Such developmental regulation by plastid presumably contributes to preventing the wasteful expansion of lamina with low photosynthetic activity.
Citation: Tameshige T, Fujita H, Watanabe K, Toyokura K, Kondo M, Tatematsu K, et al. (2013) Pattern Dynamics in Adaxial-Abaxial Specific Gene Expression Are Modulated by a Plastid Retrograde Signal during Arabidopsis thaliana Leaf Development. PLoS Genet 9(7): e1003655. https://doi.org/10.1371/journal.pgen.1003655
Editor: Xuemei Chen, University of California Riverside, United States of America
Received: November 6, 2012; Accepted: May 27, 2013; Published: July 25, 2013
Copyright: © 2013 Tameshige et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry for Education, Culture, Sports, Science, and Technology (MEXT) of Japan (No. 19060004) and a Grant-in-Aid for Creative Scientific Research from the Japan Society for the Promotion of Science (No. 19GS0315) to KOk. TTa and KTo were supported by a JSPS Fellowship from the Japan Society for the Promotion of Science (TTa, No. 21-1024; KTo, No. 20-2221). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
The expansion of a flat organ from an undifferentiated organ primordium provides an excellent model for studying the dynamics of formation and maintenance of gene expression domains. In the case of wing development in Drosophila, the wing primordium (wing disc) is subdivided into dorsal and ventral compartments. Each compartment specifically expresses key genes determining the future pattern of tissue growth and cell differentiation. Cells in each compartment are related by lineage and they are prevented from crossing the dorso-ventral boundary –.
In the case of leaf development in the model plant Arabidopsis thaliana, previous studies have revealed that several genes are expressed in an adaxial- or abaxial- specific manner. Their specific expression patterns are required for lamina expansion with adaxial-abaxial asymmetric cell differentiation (see – for review). It has been considered that their expression patterns are established during leaf initiation, that is, stages P0 to P1, and maintained during later stages –. However, previous studies have not focused on whether the gene expression states are maintained in each cell lineage as in the case of the fly wing. The adaxial-specific genes are three Class III Homeodomain-Leucine Zipper genes, PHABULOSA (PHB), PHAVOLUTA and REVOLUTA (REV) (PHB-like genes hereafter) –, and a LOB-domain family gene, ASYMMETRIC LEAVES2 . The abaxial-specific genes are four YABBY family genes, including FILAMENTOUS FLOWER (FIL) –, three KANADI genes (KANs) ,  and two AUXIN RESPONSE FACTOR genes (ARFs) , . In addition to such transcription factor genes, small regulatory RNAs are also distributed in an adaxial- or abaxial-specific manner. The microRNA miR165/166 represses the expression of PHB-like genes through mRNA cleavage in the abaxial region . The trans-acting small interfering RNAs targeting the two ARFs (tasiR-ARFs) also repress their targets through mRNA cleavage in the adaxial region , . Especially, the intercellular mobility of these small RNAs has been recently emphasized as the key feature to formation of spatial gene expression patterns , , .
The expression patterns of these transcription factors and small RNAs are considered to be the results from complex regulatory networks among themselves though many parts of the networks are yet to be elucidated . Nonetheless, it has been discussed that the adaxial- and abaxial-specific expression domains of such genes are separated and maintained owing to the mutual repression between these adaxial- and abaxial-specific genes via direct transcriptional repression, mRNA degradation and other negative regulations –, . It is highly likely that intracellular mutual repression between two (groups of) genes allows each cell to express only one (group of) gene(s) and maintain the gene expression state. On the other hand, it is not necessarily likely that the intercellular mutual repression between the genes contributes to the maintenance of the gene expression domains. While such intercellular effects prevent a cell from misexpressing the other genes within one gene expression domain, both the two domains might not be maintained for a long time because such intercellular effects might change the gene expression state of the cells on the domain boundary. This speculation is consistent with many studies showing that when two mobile factors decrease each other's quantity, the boundary between their distribution domains shifts in theory and real observations –.
In this study, we first performed computer simulations of a simple mathematical model assuming mutual repression between two factors representing the adaxial- and abaxial-specific genes. These simulations showed that the boundary position between their expression domains is not necessarily maintained, but might shift toward one end when the repression is mediated by mobile factors.
It has been described previously that the FIL-expression domain is separated from the expression domain of PHB-like genes, or the miR165/166-free domain, with no or a little overlap in primordia of leaves , flowers , cotyledons  and sepals . Orthologous genes of FIL and PHB also show similar complementary expression patterns in Antirrhinum  and Cabomba . Though the previous studies have not clearly shown whether or not the boundary position between these domains changes during leaf development, it has been reported that FIL-expression domains differ at different developmental stages of leaves , , . While the Arabidopsis leaves basically consist of six cell layers: the adaxial epidermis, four layers of mesophyll and the abaxial epidermis, FIL is initially expressed in the whole leaf at approximately the P0 stage, and then restricted to the abaxial four cell layers at later stages , . This expression domain is further restricted to the three abaxial cell layers at approximately the P6 (sixth youngest leaf) stage . The similar gradual restriction of the FIL expression to the abaxial cells has been reported in tomatoes .
Here we characterized in detail how FIL-expression and miR165/166-free (presumptively PHB-like genes expression) domains change during leaf development by careful observations of the gene expression markers and by lineage analysis of FIL-expressing cells. The results showed that all leaf founder cells express FIL and have miR165/166 activity, but the FIL-expressing cells become miR165/166-free cells sequentially from the adaxial to the abaxial side after leaf initiation. In other words, the boundary between FIL-expression and miR165/166-free domains shifts from the adaxial to the abaxial side similarly to the above computer simulation.
Our genetic analyses demonstrated that excessively fast shifting of the boundary is associated with narrow lamina formation and excess adaxialization in cell differentiation, whereas excessively slow shifting of the boundary is associated with narrow lamina formation and excess abaxialization. Furthermore, detailed analyses of the mutant enlarged fil expression domain2 (enf2) revealed that the boundary shifting is retarded by the GENOMES UNCOUPLED1- (GUN1-) dependent mechanism when plastid (chloroplast) gene expression machinery is compromised by inhibitor treatments and genetic mutations. GUN1 is an indispensable factor for the plastid-nucleus communication system known as retrograde signaling (see – for review). Therefore, our results strongly suggest that the GUN1-dependent plastid retrograde signal regulates leaf morphogenesis by affecting the dynamic change in the FIL-expression and miR165/166-free domains in leaf primordia.
From physiological point of view, the main advantage of lamina expansion is the efficiency in light reception for photosynthesis, thus depends on the functional integrity of plastids. On the other hand, from developmental viewpoint, the lamina expansion depends on the adaxial- and abaxial-specific genes in leaf primordia. We discuss how the link between adaxial-abaxial gene expression and plastid condition contributes to plant growth and development.
A Boundary between Two Gene Expression Domains Easily Shifts when Their Products Are Mobile and Repress Each Other's Expression
To know the theoretical stability of neighboring two gene expression domains when the two (groups of) genes repress each other's expression, we examined the domain stability by numerical analysis. Here we suppose a situation where
- Two gene products: AD and AB, are produced accordingly to the production functions f1 and f2, respectively, and degraded accordingly to the degradation functions g1 and g2, respectively, within each cell.
- AD represses the production and promotes the degradation of AB, on the other hand, AB represses the production and promotes the degradation of AD. Therefore, f1 and f2 are decreasing functions, and g1 and g2 are increasing functions (Figure 1A, S1A, S1B).
- When only a cell is considered, the cell can predominantly express either of AD or AB in a stable gene expression state due to the mutual repression between AD and AB. However, both expression states are stable. (Figure 1B)
- Cells are aligned in one-dimensional space representing the adaxial-abaxial axis.
- AD and AB are uniformly distributed within each cell and moves between neighboring cells in a gradient-dependent manner like the simple diffusion. (Figure 1A)
(A) Schematic illustration of the model of mutual repression and mobility. (B) The phase plane of the mutual repression system. Blue and red lines indicate nullclines of equations (1) and (2), respectively, without the diffusion terms. The filled circles with magenta and green indicate the stable steady states, named the AD-expressing state and the AB-expressing state, respectively. The open circle is an unstable steady state. (C–E) Simulation results of the mathematical model using three parameter sets: the symmetric parameter set between AD and AB (C); the asymmetric parameter sets (D, E). The parameter values are set to be p1 = p2 = 0.1, r1 = r2 = 2.0, d1 = d2 = 0.1, c1 = c2 = 2.0 and DAD = DAB = 0.1 (C), except for r2 = 1.8 (D), r1 = 1.8 (E).
Thus, the dynamics of AD and AB in cell “i” are formulated as (1)(2)where DAD and DAB are constant diffusion coefficients of AD and AB, respectively. For simplicity, the functions f and g are described as basal independent constants (the first terms) plus the Hill equations (the second terms).(3)(4)(j = 1, 2)
where pj is the basal production rate, rj is the inhibitory effect of the production rate (e.g., the effect of transcriptional repression), dj is the basal degradation rate and cj is the promotion strength of the degradation rate (e.g., the effect of mRNA cleavage) (Figure S1A, S1B). Such a mutual repression dynamics has the AD-expressing and the AB-expressing states as stable steady states with a certain range of parameter values (e.g., p1 = p2 = 0.1, r1 = r2 = 2.0, d1 = d2 = 0.1 and c1 = c2 = 2.0, Figure 1B) when only a single cell is considered (Figure 1B).
After such a bistable mathematical model was developed, we lay AD-expressing cells and AB-expressing cells side by side and simulate the time evolution of AD and AB expression. Here we consider only three AD-expressing cells and three AB-expressing cells as the initial states. The results are shown in Figure 1C–E. In the cases where parameters are symmetric between AD and AB (i.e., p1 = p2, r1 = r2, d1 = d2, c1 = c2 and DAD = DAB), the expression domains is maintained, in other wards, the initial domain boundary is fixed (Figure 1C). On the other hand, when a parameter is changed from such symmetric parameter set to asymmetric one, one gene expression domain expands and the other shrinks, namely, the domain boundary shifts toward the either end (e.g., p1 = p2, r1>r2, d1 = d2, c1 = c2 and DAD = DAB for Figure 1D, p1 = p2, r1<r2, d1 = d2, c1 = c2 and DAD = DAB for Figure 1E) (see also Figure S1C–F).
These simulations give examples of the known mathematical rule that negatively interacting mobile factors easily cause boundary shifting between their distribution domains , and suggest that this rule can be applied to molecular-biological systems including the regulatory network among the adaxial- and abaxial-specific genes in leaf primordia.
The Boundary between FIL-expression and mir165/166-free Domains Shifts
The almost exclusive relationship between the abaxial FIL-expression domain and the adaxial miR165/166-free domain has been characterized well , –. However, whether or not these domains are simply maintained during leaf development had not yet been explicitly demonstrated. If the domain separation is due to mutual repression between miR165/166 and PHB-like genes, FIL and PHB-like genes and between upstream regulators for these genes, the domain boundary might not be necessarily maintained.
To characterize in detail the spatio-temporal patterns of the FIL-expression and miR165/166-free domains during leaf development, we observed these domains at a series of leaf developmental stages. The FIL-expression and miR165/166-free domains were visualized by two fluorescent markers, FILpro:GFP (green fluorescent protein driven by the FIL promoter) and 35Spro:miYFP-W (miR165/166-sensor yellow fluorescent protein driven by the Cauliflower Mosaic Virus 35S promoter), respectively, as previously described .
Before leaf initiation (around P0 stages), FIL was likely expressed in all the leaf founder cells (Figure S2C, S2D), as previously reported , , and miR165/166 activity was higher in these cells than in the surrounding cells (Figure S2C, S2D). However, it was difficult to determine whether the FIL-expressing and miR165/166-active cells are all leaf founder cells because of the absence of a marker to distinguish those cells from the remaining meristem cells in which FIL expression and miR165/166-activity were not detected. Just after a leaf primordium initiated but before the six cell layers become obvious (P1 stage, approximately 20-µm-long), most leaf cells appeared to express FIL and have miR165/166 activity with the exception of only a few adaxial epidermal cells (Figure S2E, S2F).
At all of the developmental stages after the leaf initiation, the two domains were separated with not more than one-cell-width overlaps (Figure 2A–C, S2A, S2B). We hereafter refer to the boundary between FIL-expression and miR165/166-free domains as the FMB. In 50-µm-long primordia (P1–P2 stages), the FIL-expression and miR165/166-free domains occupied four to five abaxial cell layers and one to two adaxial cell layers, respectively, within the six cell layers (Figure 2A). It should be noted that FIL expression was detected in the distal tip cell (Figure 2A arrowhead) and the neighboring adaxial cell which presumably corresponds to future distal adaxial epidermis. Thus, FMB position was relatively adaxial rather than exactly the middle of the primordium at such early stages.
(A–F) Confocal images of longitudinal (A–C) and transverse (D–F) sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression patterns at different stages: 50-µm-long (A), 200-µm-long (B) and 300-µm-long (C–F). Lower schematic illustrations represent each boxed region in (A–C). (D–F), A series of sections from a leaf of approximately 300 µm in height. The approximate heights of the observation plane from the leaf base are indicated in (D–F). Arrowheads indicate the distal (A–C) and marginal (D–F) tip cells. (G–R) Confocal (G, Q, R) and stereoscopic (H–P) images showing VENUS expression patterns (yellow and yellow-green) of the FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS system in the third leaves of 12-day-old plants. The timing of DEX treatment for CRE/loxP recombination is indicated at the bottom left of each panel. The confocal imaging planes are a transverse section of a shoot apex (G) and third leaves (Q, R). The red color represents chlorophyll fluorescence. “+” marks the meristem center in all figures. Scale bars represent 50 µm (A–G), 1 mm (H–P) and 100 µm (Q, R). ad, adaxial side; ab, abaxial side.
When the leaf reached 300 µm in length (P6–P7 stages), the FIL-expression domain was restricted to one to two cell layers in the distal part and approximately three cell layers in the proximal part of the leaf (Figure 2C–F). On the other hand, the miR165/166-free domain in this stage was expanded compared to those in the early stages keeping the slight overlap with the FIL-expression domain. (Figure 2C–F). Thus, FMB position was more abaxial at these stages than at the early stages. In regard to the marginal epidermis, the elongating marginal tip cells but not the neighboring adaxial cells expressed FIL in the distal part (arrowheads in Figure 2D, S2G–J). In contrast, the marginal tip cells and the neighboring cells still expressed FIL in the proximal part where marginal cell elongation is not evident yet (arrowheads in Figure 2F, S2G).
During the later stages, though the expansion of the miR165/166-free domain and restriction of the FIL-expression domain continued to some extent, FIL expression was kept in the elongating margin cells, whole abaxial epidermis and most of the abaxial-most mesophyll even after the leaves exceed 1 mm in length and the round lamina morphology is developed (Figure S2K–P). However, the GFP signal intensity of FILpro:GFP gradually reduced to a level not enough to distinguish FIL-expressing cells clearly from the other cells at further later stages (data not shown).
The FMB position changes suggest that leaf cells switch from the FIL-expressing state to the miR165/166-free state, and the switch occurs sequentially from the adaxial to the abaxial cell layers and also from the central to the marginal cells within a layer.
FIL Expression Is Repressed Sequentially from the Adaxial Central Cells to the Abaxial Marginal Cells
Despite the clear abaxial shifting in FMB position, there remained a possibility that the switch in gene expression does not occur but gene expression states are just maintained in all cell lineages. If this were true, the FMB shifting should be attributed to rapid proliferation of the adaxial cells. To address this point by tracing the lineages of FIL-expressing cells, we used the dexamethasone- (DEX-) inducible CRE/loxP recombination system , which has recently been used in planta for cell lineage tracing , . Our system consists of two constructs: FILpro:CRE-GR and 35Spro:loxP-Ter-loxP-VENUS. 35Spro:loxP-Ter-loxP-VENUS does not express VENUS gene without FILpro:CRE-GR because of the transcriptional termination sequence (Ter) placed between Cauliflower Mosaic Virus 35S promoter (35Spro) and VENUS regions. However, this construct can conditionally generate a marker gene, 35Spro:VENUS (35Spro:loxP-VENUS in a precise description), only in the FIL-expressing cells by DEX-dependent and CRE-mediated DNA recombination at the two loxP sites. Thus, the FIL-expressing cells and their progenies are permanently marked by the fluorescence of VENUS retained in the endoplasmic reticulum after DEX application (see Text S1 and Figure S3 for the efficiency and specificity of this CRE/loxP system). If the FMB shifting is just due to the rapid proliferation of adaxial cells, DEX application at any developmental stage results in the same VENUS expression pattern. In contrast, if FMB is shifting depending on the gene expression switch, DEX applications at different developmental stages generate various expression patterns of VENUS corresponding to those of the FILpro:GFP at the stages of DEX application.
To reveal the FIL-expression dynamics during leaf development, DEX was applied to the FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS plants from a series of developmental stages and the VENUS expression patterns were observed in mature leaves. For this analysis, the third true leaves were analyzed because their growth rate is well characterized under our laboratory conditions. Fluorescent images of the adaxial-side view were captured to elucidate the quasi-three-dimensional expression patterns of VENUS in the leaves (Figure 2H–O) because the areas of strong and intermediate fluorescence intensities correspond to those of VENUS expression in the adaxial epidermis and the adaxial-most mesophyll, respectively (Figure S4). When the plants had been treated with DEX since seed germination (day 0), VENUS expression was detected either in all leaf cells or in almost all leaf cells except for a small region in the adaxial central part of the epidermis (Figure 2G–I, 2Q). DEX treatments from just before third leaf initiation (day 3) resulted in the same VENUS expression patterns as the treatments since day 0 did (Figure 2J). Given that FIL-expression patterns does not vary among the plants, such two patterns of VENUS expression likely reflect that FIL expression is initially induced in all leaf founder cells at the P0 stage and immediately repressed in the adaxial central epidermal cell(s) that does not accomplish the CRE/loxP recombination to generate the 35Spro:VENUS gene in some cases.
DEX treatment from day 4, when the leaf reaches approximately 20- to 50-µm-long (P1–P2 stage), generated slightly more VENUS-negative cells in the adaxial central part than DEX treatment beginning on day 0 or day 3 (Figure 2K, S5E). However, the marginal epidermis still expressed VENUS in the distal and proximal parts (Figure S4A, S4D) showing a good agreement with the aforementioned FILpro:GFP expression pattern at the comparable stage (Figure 2A, S2E, S2F). In a series of DEX treatments beginning from day 4 to 8, the later the treatment was performed, the more the VENUS expression area was restricted to the abaxial cell layers and to lateral and proximal parts of the two adaxial cell layers (Figure 2K–O, S4, S5E). In regard to the marginal epidermis, VENUS expression is detected in the proximal part, but not in the distal part when DEX was applied from day 6 (Figure 2M, S4B, S4E, S4F). Because the third leaf reaches to 200 to 300 µm in length (approximate P6 stage) by day 6, this VENUS expression pattern is in good agreement with the FILpro:GFP expression pattern in such leaves (Figure 2D–F). When DEX was applied from day 8 (approximately 1,000-µm-long, P9–P10 stage), VENUS expression in the two adaxial cell layers almost disappeared (Figure 2O, S4C, S5E), but two or three abaxial cell layers still expressed VENUS (Figure 2P, 2R, S4G, S4H), showing a good agreement again with the FILpro:GFP expression pattern (Figure S2K–P). In summary, the series of DEX treatments generated a variety of the VENUS expression patterns each of which highly correlates with each FILpro:GFP expression pattern at the corresponding stage. Therefore, it is indicated that FMB shifting is mainly caused by the gene expression switch from the FIL-expressing to the non-FIL-expressing state in each cell.
A Mutant enf2 Shows Slow FMB Shifting
To gain insights into the regulatory mechanisms for FMB shifting, we sought mutants defective in the shifting process. A candidate of such mutants, enlarged fil expression domain2 (enf2) was isolated from a genetic screen for altered FILpro:GFP expression patterns . Whereas the FIL-expression domain in the wild type did not surround the center provascular cells (Figure 3A), the domain in this mutant was abnormally large, frequently surrounding the provascular cells in around the P5 stage (Figure 3B).
(A–E) Confocal images of transverse sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression in the wild-type (A) and enf2 (B–E) leaf primordia. The arrowheads indicate the provascular cells. (C–E), A series of sections from a leaf of approximately 300 µm in height. The approximate heights of the observation plane from the leaf base are indicated. The comparable WT data are Figure 2D–F. (F) FIL-expression area sizes (%, y-axis) at different stages (grouped by section area sizes, x-axis) of the wild-type and enf2 leaf primordia. Bars indicate standard errors. n.s., not significantly different; *, significantly different (p<0.05, t-test) between the wild type and enf2. (G, H) Seedlings of the wild type and enf2. (I, J) Scanning electron microscope images of leaf sections from the wild type and enf2. Scale bars represent 50 µm (A–E, I, J) and 1 mm (G, H). WT, wild type.
To characterize the dynamics of FIL-expression and miR165/166-free domains, the markers FILpro:GFP and 35Spro:miYFP-W were observed in various developmental stages of enf2 leaf primordia. Whereas the two domains were almost separated at all stages in this mutant as in the wild type, the domain sizes were different between enf2 and the wild type especially in later developmental stages (Figure 3A–E, S5A–D). In small leaf primordia, the size of the FIL-expression domain in enf2 was comparable to that in the wild type (Figure 3F, S5A, S5C). By contrast, in relatively large leaf primordia, the FIL-expression domain in enf2 was significantly larger than that in the wild type (Figure 3F, S5B, S5D). For example, in 300-µm-long primordia of enf2, the FIL-expression domain was restricted to two to four abaxial cell layers (Figure 3C–E). Such a domain size was larger than that in the wild type at the same developmental stage (Figure 2D–F, 3C–F) suggesting the slower FMB shifting than in the wild type. Nonetheless, the FIL-expression domain in enf2 at this stage was smaller than that in the early stages (Figure S5C, S5D), suggesting that the FMB shifting did occur even in this mutant. Furthermore, the FILpro:CRE-GR and 35Spro:loxP-Ter-loxP-VENUS system revealed that FIL expression is initially induced in all of the leaf cells and gradually restricted during later stages in enf2 (Figure S5F–N). This analysis also showed that the sizes of the VENUS-negative area in leaves treated with DEX from day 3 did not significantly differ between the wild type and enf2 (the left data points in Figure S5E). This suggests that the earliest repression of FIL expression in the adaxial central epidermis at the P0 stage is not affected in enf2. The results from marker observation and lineage tracing can be interpreted as showing that the FMB shifting occurs in enf2, but more slowly than in the wild type.
Altered FMB Shifting Is Associated with Defects in the Lamina Expansion and the Adaxial-Abaxial Cell Differentiation
Mature leaves in enf2 are pale green, more serrated and narrower than those in the wild type (Figure 3G, 3H). In some cases, enf2 formed needle-like leaves lacking trichomes at a frequency of less than one percent (Figure S5O). These narrow and needle-like morphologies are similar to those of abaxialized leaves, including the leaves of 35Spro:FIL plants , , rev recessive mutants harboring another enhancer mutations , ,  and 35Spro:MIR165 plants –. Further observations of leaf anatomy by scanning electron microscopy revealed that adaxial mesophyll cells in enf2 were not as densely packed and columnar shape as those of the wild type but had air space and a bumpy cell surface looking more like that of the abaxial spongy mesophyll of the wild type (Figure 3I, 3J). In summary, enf2 leaves are partially abaxialized with respect to the lamina morphology and mesophyll differentiation, suggesting that slow FMB shifting results in narrow lamina formation and abaxialized mesophyll.
To know the relationship between the leaf morphological features and the FMB shifting speed, we analyzed FMB shifting in phb-1d heterozygous (phb-1d/+) plant, which is well known for the partially adaxialized leaf phenotype of narrow and cup-shaped leaves  (Figure 4H, 4I) with excessive densely packed and smooth mesophyll cells  (Figure 4J, 4K). When FILpro:GFP 35Spro:miYFP-W markers were introduced into phb-1d/+, the FIL-expression and miR165/166-free domains were separated as in the wild type (Figure 4A–E). The FIL-expression domain occupied three to four abaxial cell layers in the early primordium (approximately 50-µm-long) (Figure 4A, 4B; see also Figure 2A, S5A) and two to zero cell layers in the later primordium (approximately 300-µm-long) (Figure 4C–E; see also Figure 2D–F). The FILpro:CRE-GR and 35Spro:loxP-Ter-loxP-VENUS system revealed that FIL is expressed throughout the leaf at the initiating stages, even in phb-1d/+ plants (Figure 4F, 4G). Therefore, the speed of FMB shifting is greater in phb-1d/+ than in the wild type, suggesting that fast FMB shifting results in narrow and abnormal lamina formation and excess adaxialization of mesophyll.
(A–E) Confocal images of longitudinal (A) and transverse (B–E) sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression in phb-1d/+ leaf primordia. (C–E), A series of transverse sections from a leaf of approximately 300 µm in height. The comparable WT data are Figure 2D–F. (F, G) VENUS expression pattern (yellow and yellow-green) of FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS in phb-1d/+. A transverse section of leaf primordia (F) and a stereoscopic image of mature leaf (G). (H, I) Seedlings of the wild type and phb-1d/+. (J, K) Scanning electron microscope images of leaf sections in the wild type and phb-1d/+. Scale bars represent 50 µm (A–F), 1 mm (G–I) and 100 µm (J, K).
ENF2 Encodes a Plastid-Localized Protein
A positional cloning approach found a single base substitution at the second exon terminus (enf2-1 allele) of the gene AT1G31410 in the enf2 genome (Figure 5A). Because the mutant phenotype was rescued by introducing the wild-type genomic fragment of this gene (Figure S6A–C), we concluded that ENF2 is AT1G31410. The enf2-1 mutation results in no amino acid substitution when the mRNA is spliced as in the wild type. However, RT-PCR analysis revealed that the mutation leads to unusual splicing events (Figure 5B) that generate premature stop codons in the majority of the mRNA (data not shown). Thus, the amount of functional ENF2 mRNA is reduced in this mutant. However, the mutant of another enf2 allele (enf2-2) found in the SALK T-DNA insertion lines (Figure 5A) showed whiter and narrower leaves than enf2-1 and was seedling lethal (Figure S7B). In addition, ENF2 mRNA with the normal exon junctions was detected from the enf2-1 mutant as a small peak in an electropherogram of the RT-PCR product (Figure S7A). Therefore, viable enf2-1 mutant retains some ENF2 function and is a weak allele that is useful for analyses of ENF2 function without side effects of lethality.
(A) Schematic representation of the ENF2 gene. Boxes represent exons. The untranslated regions, the putative transit peptide and the PotD/F homology domain are highlighted in gray, green and blue, respectively. The mutations in the enf2-1 and enf2-2 alleles are indicated. LB, left boarder of T-DNA. Blue, pink and orange arrows indicate the primers for RT-PCR analysis (B). (B) RT-PCR using the primers represented as the blue and orange arrows (left), the blue and pink arrows (right). (C–E) Confocal images showing VENUS-ENF2 (green, C), chlorophyll (magenta, D) fluorescence and both (E) in petiole cells. (F, G) Confocal images showing a transverse (F) and longitudinal (G) sections of a shoot apex expressing ENF2pro:VENUS-ENF2 (green). Scale bars represent 50 µm (C–G).
We found an enf2 enhancer mutation (ene hereafter) in the process of cloning ENF2. The existence of this enhancer locus was indicated by the fact that some F2 plants from the cross between the wild type and enf2 showed a milder mutant phenotype than the parental enf2 in terms of the leaf morphology, color (Figure S7D, S7F) and FIL-expression pattern (Figure S7G). The positional cloning approach and rescue experiments identified a missense mutation in AT1G80070/SUS2 as the ene mutation (Figure S7H–L). This gene product is a homolog of yeast Prp8, which plays an important role in recognizing exon-intron junctions during mRNA splicing . To examine the possibility that the ene mutation weakens ENF2 function by affecting the splicing efficiency of ENF2 mRNA in the enf2-1 mutant, we compared the amounts of normally spliced ENF2 mRNA in the enf2-1 single mutant and the enf2-1 ene double mutant. The electropherogram of the ENF2 RT-PCR products revealed that enf2-1 ene contained less wild-type mRNA than enf2-1 (Figure S7A). Moreover, the ene single mutant was indistinguishable from the wild type in leaf morphology, color (Figure S7C, S7E) and FIL-expression pattern (Figure S7G). These data suggest that the ene mutation enhances the enf2 phenotype by decreasing the splicing efficiency of the enf2-1 allele, and we continued to use the enf2-1 ene double mutant (described as enf2 again below) for further analyses as a plant defective in ENF2 function.
To analyze expression and subcellular localization of ENF2 protein, we created transgenic plants expressing VENUS-ENF2 fusion gene under the control of the ENF2 promoter. This transgene, ENF2pro:VENUS-ENF2, was able to rescue enf2 (Figure S6A, S6B, S6D), indicating that ENF2pro:VENUS-ENF2 can confer authentic ENF2 expression and that VENUS-ENF2 is functional. VENUS-ENF2 was localized in chloroplasts (Figure 5C–E), suggesting that ENF2 is localized in chloroplasts. This result is consistent with recent reports of proteomic analyses that the Arabidopsis ENF2 protein and its maize homolog were detected in the plastid (chloroplast) fraction , . To clarify ENF2 function, the ENF2pro:VENUS-ENF2 expression pattern was analyzed. VENUS-ENF2 was expressed throughout the shoot apical meristem and leaf primordium (Figure 5F, 5G). Such a broad expression pattern of ENF2 is consistent with a previous transcriptomic study in which the ENF2 mRNA was detected from the FIL-expressing part and the central-zone of the shoot apical meristem  (http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi).
While there is no previous report showing the protein function of ENF2 and the homologs in plant, BLAST search (protein blast in NCBI: http://blast.ncbi.nlm.nih.gov/Blast.cgi) found that ENF2 amino acid sequence shows low similarity to those of two bacterial proteins, PotD (44% similarity) and PotF (46% similarity), which specifically bind to polyamines and transport them into cells . The amino acid residues indispensable for the polyamine binding of PotD and PotF are widely conserved among the bacterial homologs  (Figure S8 red). However, the corresponding regions are substituted in ENF2 by dissimilar residues that are conserved only among green plant lineages and cyanobacteria (Figure S8 green). The similarity and difference among the homologous proteins might imply that ENF2 in plastids has some functions similar to but distinct from those of PotD and PotF in bacteria.
ENF2 Is Required for Chloroplast Development and Plastid Gene Expression in Leaf Primordia
The mutant phenotypes of pale green (enf2) (Figure 3H) and white (enf2-2) (Figure S7B) color and the localization of ENF2 protein to plastid imply a role of ENF2 in chloroplast development. To characterize the effect of the enf2 mutation on chloroplast development, we observed the plastid inner structures by transmission electron microscopy (Figure S9A–F). Mature enf2 leaves had normal-looking chloroplasts because they showed highly stacked thylakoid membranes and large starch granules, as did the wild-type leaves (Figure S9C, S9F). In contrast, plastids in the enf2 leaf primordia had less-developed inner structures than those in the wild type in comparable stages (Figure S9B, S9E). Taken together with the similarity of the proplastid structure in the meristems of wild type and enf2 (Figure S9A, S9D), the chloroplast development is likely to be delayed in the mutant.
The enf2 phenotypes of pale green appearance, narrow laminae and defective differentiation of palisade mesophyll are common defects to plants harboring dysfunctional plastidial ribosome or plastidial RNA polymerase –. To examine whether plastid gene expression is impaired in enf2, the expression levels of all of the 80 protein-coding and two rRNA genes encoded by the plastid genome were analyzed by Quantitative Reverse Transcription PCR (qRT-PCR). On average, the expression levels were reduced to 49.0% of the wild type, with a range of 93.2% to 23.7% (Figure S9G). Because the amounts of rRNA were also reduced (the right-most two bars in Figure S9G), plastid gene expression might be globally down-regulated, not only at the RNA level but also at the level of translation in enf2.
It has been known that the plastid gene expression profile is affected when chloroplast development is inhibited by external stresses  and that inhibition of plastid gene expression by chemical treatments or genetic mutations leads to defective chloroplast development , , , . ENF2 is also important for chloroplast development and plastid gene expression, though it is unclear which of the chloroplast development and plastid gene expression is primarily affected in enf2.
Inhibition of Plastid Gene Expression Leads to Retardation of FMB Shifting
The abnormalities in plastid condition suggest the involvement of plastid function in regulating the FMB shifting. To test whether the defective chloroplast development is sufficient to retards FMB shifting and lead to narrow lamina development, chloroplast development was inhibited in the wild type by norflurazon treatment and dark growth condition. Norflurazon is an inhibitor of carotenoid biosynthesis  that bleaches seedlings by causing oxidative damaging of plastids in the light. Although application of 0.25 µM norflurazon caused leaf bleaching, the leaves had roundly expanded lamina even at 25 µM concentration (Figure S10A). Dark-grown seedlings were etiolated and lacking in chloroplast but formed round lamina (Figure S10B). The narrow appearance of the leaves was due to their elongated petioles (Figure S10C). Neither norflurazon nor dark condition led to as clear alterations in the FILpro:GFP 35Spro:miYFP-W expression patterns (Figure S10D, S10E) as enf2 showed. These results indicate that the FMB shifting and lamina morphology are not necessarily affected by the inhibition of chloroplast development.
To know whether the plastid gene expression activity affects the FMB shifting and lamina development, we inhibited the plastid translation in the wild type by lincomycin and erythromycin treatments. Both lincomycin and erythromycin specifically inhibit plastidial ribosome without clear effects on cytosolic and mitochondrial ribosomes . At concentrations of 100 µM, these chemicals bleached seedlings, and narrow and filamentous leaf formation was observed at concentrations greater than 200 µM (Figure 6A, 6B). In the leaf primordia of the lincomycin- and erythromycin-treated plants, FILpro:GFP 35Spro:miYFP-W marker expression patterns showed that the FMB positions were close to the adaxial side, similarly to that in enf2 (Figure 6E, 6F). The effects of lincomycin and erythromycin treatments on the lamina morphology and FMB position indicate that enf2-like phenotype is regenerated by inhibition of protein synthesis in plastids.
(A–D) Seedlings of lincomycin- and erythromycin- treated wild type, and untreated flv mutant and wild type. (E–H) Confocal images of transverse sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression in leaf primordium of each above plant. The arrowheads indicate the provascular cells. Scale bars represent 1 mm (A–D) and 50 µm (E–H). Lin, lincomycin; Ert, erythromycin.
Though the lincomycin- and erythromycin-treated plants showed enf2-like phenotype in morphology, they were albino and seedling lethal suggesting that plastid function is more extensively affected in these plants than in enf2 which is pale green and viable. To examine whether the impaired plastid gene expression is responsible for defective FMB shifting independently of abnormal photosynthesis and developmental arrest, we sought a mutant impaired in plastid gene expression machinery that exhibits narrow lamina with a mild phenotype in color and viability. For this purpose, the mutant flavodentata (flv) was selected because the FLV gene has been shown to encode a plastid-localized PPR protein required for RNA editing of the rpoC1 mRNA, which encodes a subunit of plastid-encoded RNA polymerase (I. Small, personal communication), and because flv, which is allelic to defectively organized tributaries4  (I. Small, personal communication), is viable and known for its narrow serrated leaves that are pale green in color ,  (Figure 6C). Observations of FILpro:GFP 35Spro:miYFP-W marker expression in flv revealed that the FMB position was close to the adaxial side, similarly to that of enf2 (Figure 6G). This result supports the role of plastid gene expression in FMB shifting and lamina expansion, and shows the separable nature of this role from the development of the photosynthetic apparatus and lethality.
From the result that enf2-like phenotype was regenerated by lincomycin and erythromycin treatments and flv mutation but not by norflurazon treatment and dark growth condition, it is suggested that the impaired plastid gene expression is the key to the defective FMB shifting and lamina morphology in enf2. However, it has been known that the plastid gene expression profile of the whole seedling RNA is also globally fluctuated by norflurazon treatment , . To examine whether or not the alterations in plastid gene expression in the shoot apex is less severe in the norflurazon-treated plants than in enf2, we also quantified the expression levels of several plastid genes in these plants. The result showed that not only the photosynthesis-related genes (rbcL, psaA, psaB, psbA, psbE) but also the genes for transcription (rpoA, rpoB, rpoC1, rpoC2) and for translation (rrn16S, rrn23S) are down-regulated as severely as or more than in enf2 (Figure S10F). This means that the expression levels of plastid genes in the whole shoot apex tissue are not always linked to the enf2-like phenotype. Nonetheless, simultaneous treatment of norflurazon and lincomycin resulted in narrow lamina formation (Figure S10G). This result indicates that the norflurazon-treated plants are not insensitive to, but show the developmental response to, the inhibition of plastid gene expression. Therefore, it is likely that the enf2-like phenotype is caused by direct inhibition of plastid gene expression machinery rather than by the reduced levels of plastid gene expression.
To further characterize the similarity between enf2 mutation and the lincomycin treatment, enf2 was also treated with lincomycin. When enf2 was treated with 150 µM of lincomycin, filamentous leaves were formed in more than 90 percent of seedlings (Figure S10H). In contrast to enf2, wild type required 450 µM of lincomycin to form such leaves at a similar frequency (Figure S10I). This result supports that enf2 mutation leads partial impairment in plastid gene expression as a certain concentration of lincomycin does, thus they both retard FMB shifting and inhibit lamina expansion.
The Retardation of FMB Shifting by Plastid Depends on the GUN1-dependent Retrograde Signal
The mechanism with which nuclear gene expression levels respond to the plastid condition is called the plastid retrograde signal. It has been known that changes in plastid gene expression and other plastid conditions affect the expression levels of nuclear genes, including photosynthetic genes, through the GENOMES UNCOUPLED1- (GUN1-) dependent retrograde signal (see – for review). In addition, another specific retrograde signal is also known to couple the nuclear gene expression with tetrapyrrole biosynthetic activity but not with plastid gene expression. Other GUN genes (GUN2, 3, 4 and 5) are involved in this specific pathway , .
To examine the involvement of known retrograde signals in the plastid effect on the lamina expansion and FMB shifting, we analyzed the responses of gun mutants to the inhibition of plastid gene expression. While gun2, 3, 4 and 5 mutants formed narrow or filamentous leaves as the wild type did (Figure S11A and data not shown), the gun1 mutant formed relatively round lamina (Figure 7A) when they were treated with lincomycin. The lincomycin-treated gun1 showed a similar FMB position to that of the untreated wild type (Figure 7D), suggesting that the unaffected FMB position in leaf primordia is the basis for the lamina expansion. In contrast to such distinct phenotype of gun1 under the lincomycin-treated condition, untreated gun1 mutant showed the leaf morphology indistinguishable from that of the wild type (Figure 7C), as previously reported , . The FMB position in gun1 leaf primordia did not differ significantly from that of the wild type at any stage of leaf development (Figure 7F, S11C). These gun1 phenotypes indicate that the GUN1 is involved in the retardation of FMB shifting and defective lamina expansion only in response to the inhibition of plastid gene expression, but not under normal plastid conditions.
(A–C) Seedlings of lincomycin-treated gun1, untreated enf2 gun1 and untreated gun1. (D–F) Confocal images of transverse sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression in leaf primordium of each above plant. The arrowheads indicate the provascular cells. Scale bars represent 1 mm (A–C) and 50 µm (D–F).
If the retarded FMB shifting in enf2 is due to the failure of plastid gene expression, additional gun1 mutation may suppress this phenotype. As expected, the enf2 gun1 double mutant, but not enf2 gun5, showed round lamina (Figure 7B, S11B). The FMB position in enf2 gun1 was similar to that of the wild type grown normally (Figure 7E, 6H). However, enf2 gun1 was different from enf2 not only in the FMB position and the lamina morphology, but also in the color and viability. The double mutant showed an almost albino phenotype and was seedling lethal (Figure 7B), suggesting that the suppressed developmental phenotypes were not due to rescued plastid condition. To confirm this point, we checked plastid gene expression levels in enf2 gun1 by qRT-PCR. Though the expression levels of the transcription-related genes (rpoA, rpoB, rpoC1, rpoC2) were similar to those in the wild type, the amount of ribosomal RNA (rrn16S, rrn23S) and other genes' mRNA were much more reduced in enf2 gun1 than in enf2 (Figure S11D). Therefore, it is likely that that gun1 mutation suppresses the enf2 phenotype not by rescuing the failure of plastid gene expression, but by diminishing the response to the abnormal plastid condition.
The albino and lethal phenotype of enf2 gun1 and the pale green and viable phenotype of enf2 suggest that the wild type allele of GUN1 is important for the viability and autotrophic growth of the plant when the plastid gene expression is accidentally impaired. This hypothesis was also supported by the observation that the wild type and gun1 plants differed in the viability after transient lincomycin treatment. After transfer from the lincomycin-containing medium to the standard medium, wild type plants produced green shoots and continued growth, whereas gun1 did not grow any longer (Figure S11E, S11F). These results indicate that GUN1 has a role in retarding FMB shifting and inhibiting lamina expansion when plastid gene expression is inhibited by internal and external damages, but the plant can adapt to such a severe situation thanks to the GUN1 function.
In this study, we revealed the following two points.
- The boundary between FIL expression and miR165/166-free domains shifts from adaxial side to the abaxial side during leaf primordial development. This boundary shifting is caused by alternative transfer of cellular gene expression profile from the abaxial-region specific to the adaxial-region specific (Figure 8A). Altered speeds of the boundary shifting are associated with the morphological changes in lamina expansion and in mesophyll differentiation.
- When plastid condition is impaired by chemical treatments and genetic mutations affecting plastidial ribosome and RNA polymerase, the boundary shifting is retarded and the lamina morphology becomes narrow (Figure 8B). These plastid effects depend on GUN1 gene (Figure 8C).
Most leaf cells express FIL and have miR165/166 activity just after leaf initiation. However, during the early developmental stages, the FIL-expressing and miR165/166-active cells switch the nuclear gene expression state to that expressing PHB-like genes, thus FMB shifts. When plastid gene expression machinery is functional (A), the gene expression switch in nuclei progress smoothly regardless whether GUN1 is functional or not. The pace of this gene expression switch is important for the full lamina expansion. When the plastid gene expression machinery is impaired (B), the GUN1-dependent retrograde signal affects the nuclei to delay or stop the gene expression switch. This plastid effect contributes to prevent the wide lamina expansion. Possibly, the GUN1-dependent retrograde signal regulates also other nuclear genes to repair the plastid condition. When the plastid gene expression machinery is impaired and the plant is devoid of the GUN1-dependent retrograde signal (C), the switch in nuclear gene expression progress normally and lamina expands despite the absence of photosynthetic activity.
These points together highlight a sophisticated developmental regulation to ensure the total photosynthetic efficiency of leaves by preventing the lamina expansion with inactive plastids. The molecular mechanisms and physiological importance of each point are discussed below.
The Mechanism and Developmental Importance of FMB Shifting
The mathematical model shows that a boundary between two gene expression domains easily shifts when the genes repress each other's expression via mobile factors. Though the whole regulatory network for the adaxial- and abaxial- specific genes is still largely unclear , this theory of boundary shifting gives a good working hypothesis for the mechanism of FMB shifting because of the following reasons. The mathematical model assumes only three points; two (groups of) genes (AD and AB) are repressing each other's expression; a cell can express only one (group) of the genes due to the mutual repression when there is no external cues fluctuating the gene expression; some of the gene products move between neighboring cells depending on the concentration gradient. Accordingly, a mutual repressive relationship is known at least between PHB-like genes and miR165/166 whereas the regulatory relationship between PHB-like genes and FIL is yet to be elucidated. PHB-like genes repress miR165/166 activity by positively regulating AGO10/PINHEAD , , by decreasing miR165/166 expression level via cytokinin signal  and possibly by activating tasiR-ARFs . The activity of miR165/166 in turn represses the expression of PHB-like genes through mRNA cleavage  and DNA methylation . In this context, the candidates of the mobile factors are miR165/166, cytokinin and possibly tasiR-ARFs. Therefore, an important suggestion from the mathematical model is that any unknown factors are not necessarily required to explain the shifting nature of FMB. Our mathematical model is also compatible with the phb-1d/+ phenotype because the shifting speed of the domain boundary toward the abaxial side is increased in the computer simulation when the AD degradation by AB is weakened (Figure S12). Such situation roughly corresponds to phb-1d/+ mutant in which the cleavage efficiency for PHB mRNA by miR165/166 is reduced  and the speed of FMB shifting is faster than in the wild type. Further comparisons between the model and real observations will be an important approach to evaluate the model and elucidate the molecular basis for the FMB shifting.
Our data suggest that the speed of the FMB shifting is important for round and wide lamina expansion (Figure 8A) because fast and slow FMB shifting were associated with narrow or abnormal lamina formation in phb-1d/+ and enf2 mutants. It has been characterized well that the functions of FIL, PHB-like genes and miR165/166 are required for the lamina growth because their loss-of-function mutations and overexpression lead to narrow lamina or needle-like leaf formation , , , –. However, a clear explanation of how FMB shifting is linked to the lamina expansion is one of the future challenges. Lamina expansion in the lateral direction requires WUSCHEL-RELATED HOMEOBOX1 (WOX1) and PRESSED FLOWER/WOX3 (PRS) expression  and local auxin biosynthesis by YUCCA genes  at the adaxial-abaxial juxtaposition domain. Therefore, the expression domains and durations of such genes for lamina expansion might be regulated in response to the stage-specific positions of FMB. To further characterize the relationship between FMB shifting and lamina expansion, an important challenges is the three-dimensional live-imaging of FMB shifting with simultaneous monitoring of cell proliferation and other genes' expression.
The Mechanism of the FMB Position Regulation through GUN1-Dependent Retrograde Signal
Our data show that chemical and genetic inhibition of plastid gene expression machinery retards FMB shifting via the GUN1-dependent mechanism (Figure 8B). It is known that the GUN1-dependent retrograde signal down-regulates photosynthetic genes in the nucleus by changing the expression levels of the transcription factor genes ABI4 and GLK1, which subsequently changes the expression levels of the downstream photosynthetic genes , , . One possible scenario is that ABI4 and GLK1 also affect the expression levels of FIL, miR165/166 and other adaxial- and abaxial-specific genes through the transcriptional regulation. Among the adaxial- and abaxial-specific genes, KAN1 and ETTIN/ARF3 are known to be up-regulated in response to the impaired plastid gene expression though the involvement of GUN1 in the up-regulation is unclear . On the other hand, it is also possible that the slow FMB shifting is a more indirect effect than such direct transcriptional regulations. For example, there are some reports pointing out that lincomycin-treated plant differently express the genes encoding cytosolic ribosomal proteins  and that some mutants of the genes for cytosolic ribosomal proteins form the partially abaxialized leaves as plastid-defective mutants do , –. Another study shows the importance of abscisic acid metabolism for the leaf morphological phenotype of a plastid-defective mutant . These reports suggest a possibility that changes in the cytosolic ribosomal proteins and abscisic acid metabolism mediate the regulation of the FMB position by the GUN1-dependent retrograde signal.
The Physiological Importance of the Leaf Developmental Regulation through GUN1-Dependent Retrograde Signal
Previous genetic studies have reported that the mutant plants harboring dysfunctional plastidial ribosomes or plastidial RNA polymerase show narrow laminae and/or defective palisade mesophyll differentiation as enf2 does –. However, the question of whether such developmental effects reflect only the inability to run the normal developmental program or a significant response to the plastid dysfunction has been unanswered. This question is partially answered by the phenotype of enf2 gun1 and lincomycin-treated gun1 because their normal FMB positions and round laminae indicate that the leaf primordia retain the ability to run the developmental program for FMB shifting and lamina expansion even when the plastids are dysfunctional (Figure 8C). This finding raises a question of whether the inhibition of such normal lamina development by the GUN1-dependent mechanism is beneficial for plant life in any respect when the plastid gene expression is impaired. The GUN1-dependent retrograde signal is considered to be the plastid-nucleus communication system to coordinate the nuclear gene expression with the changing plastid condition during chloroplast development , , . Because plastid gene expression is affected differently by various biotic and abiotic stresses  and genetic mutations in nuclei and plastids, the coordinated regulation of nuclear genes is required for the successful development of photosynthetic apparatus from non-chloroplast plastids. However, when the plastid gene expression machinery is heavily impaired at the primordial stages, it is difficult to develop a fully functional photosynthetic apparatus. In such a case, full lamina expansion is risky because the cost of the lamina formation is not compensated for by little photosynthetic product. Therefore, the inhibition of lamina expansion by the GUN1-dependent mechanism can be interpreted as the avoidance of such wasteful development. From this viewpoint, it is suggestive that the gun1 mutant becomes seedling lethal when transiently treated with lincomycin and in the enf2 mutant background. This less viable gun1 phenotype shows that the GUN1-dependent mechanism enables robust and sustainable plant development by optimizing the total photosynthetic efficiency even when plastid gene expression is impaired by internal and external fluctuations.
It is considered that the planar lamina morphology of seed plants had been evolved depending on the adaxial-abaxial asymmetry of gene expression in leaf primordia . Meanwhile, the evolutionary advantage of the planar morphology is the efficient light reception in chloroplasts for photosynthesis, thus depends on ensuring the functional integrity of plastids. From this evolutionary point of view, the problem of how the regulatory system for adaxial-abaxial gene expression pattern and lamina expansion by plastid has been evolved is as important as that of how planar lamina morphology with adaxial-abaxial asymmetry has been evolved.
Materials and Methods
Arabidopsis thaliana plants of Columbia (Col) accession with or without FILpro:GFP 35Spro:miYFP-W markers were used as the wild type. The phb-1d/+ mutant (L.er accession background) was used after crossing to Col more than four times. The enf2 mutants (enf2-1 ene and each single mutant) were isolated from the EMS-treated FILpro:GFP plants as described previously  and used for all analyses after backcrossing more than 4 times to parental line. enf2-2 (SALK_063761) was obtained from the Arabidopsis Biological Resources Center (ABRC) at Ohio State University. The flv (CS3254 from ABRC), gun1-1, gun2-1, gun3-1, gun4-1 and gun5-1 mutants ,  were previously described.
Plant seeds were sterilized and kept at 4°C in the dark for two days before sowing on 0.9% agar plates containing 1% sucrose and 0.5× Murashige-Skoog salt medium. For chemical treatments, each plate contained DEX (10 µM final concentration), lincomycin, erythromycin or norflurazon (see Results and Figures for the concentrations). For DEX treatment, growing seedlings were dipped in a DEX solution (50 µM) before being transferred to DEX plates. The plants were grown at 22°C and under continuous white fluorescent light of approximately 60 µmol photon m−2 s−1 except for the dark treatment, in which the plates were covered with aluminum foil. The phb-1d/+ mutant was grown at 16°C to moderate its phenotypic severity. Some plants were transferred to or sown in soil when seeds were needed.
Microscopy and Image Analysis
To observe GFP, YFP and VENUS marker expression patterns by confocal microscopy, plant samples were embedded into agarose gel, sectioned and observed as previously described . The height of the observation plane from the leaf base was estimated from the section thickness, the section number from the meristem-containing section and the focal plane position within the observed section. The FILpro:GFP-positive areas in leaf primordia were measured using ImageJ v1.45s (National Institutes of Health, MD) as previously described . In most of the observations, the plants were observed when the first leaf grew as big as the cotyledon.
To observe the VENUS expression area by stereoscopy, an SZX16 fluorescence stereoscope equipped with a GFP filter and a CCD camera DP72 (OLYMPUS, Japan) was used. From the acquired RGB color images, the green channel image was extracted, and the VENUS-positive areas in the adaxial epidermis and the adaxial-most mesophyll were measured using ImageJ. Normal stereoscopy images were acquired with the same stereoscope under white light illumination.
Scanning electron microscopy was performed as previously described . The frozen leaves were cracked to observe the mesophyll structure.
For plastid ultrastructure observation, 8- or 16-day-old seedlings of the wild type and enf2 were fixed by two steps in 0.05 M cacodylate buffer at pH 7.4. The first buffer contained 4% (w/v) paraformaldehyde and 1% (v/v) glutaraldehyde, and the second buffer contained 0.5% osmium tetraoxide. These fixation steps took overnight and two hours, respectively. Fixed samples were dehydrated with a series of ethanol solutions and transferred into propylene oxide. These processed samples were embedded into EPON 812 resin (TAAB Laboratories, UK). Ultrathin sections made with an Ultramicrotome (Leica, Austria) were stained by 4% uranyl acetate and 0.4% lead citrate. The ultrathin sections were examined with a transmission electron microscope H-7600 (Hitachi, Japan) at 80 kV.
Plasmid Construction and Plant Transformation
All T-DNA transformation was performed by vacuum infiltration using the Agrobacterium tumefaciens strain ASE. Transgenic plants were screened for BASTA or Kanamycin resistance. The marker genes FILpro:GFP , 35Spro:miYFP-W , FILpro:CRE-GR (see below) and 35Spro:loxP-Ter-loxP-VENUS  were introduced into each mutant by genetic cross after T-DNA transformation into the wild type (Col). Double transgenic FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS plants were obtained by crossing the single transgenic lines and analyzed in the F1 generation.
For the enf2 mutant complementation, a 6.1-kb AT1G31410/ENF2 genomic fragment, including 2647 bp upstream from the start codon and 1199 bp downstream from the stop codon, was amplified from BAC#T8E3 (from ABRC) by PCR using the primers 5′-CGGGGTACCTGATTGAGAATGTGATGAAGG-5′ and 5′-GGCTCTAGAGACCTCGGGTAAAACCC-3′. This fragment was cloned into a modified pBluescript II SK+ (Agilent Technologies, CA), transferred to a binary vector, pGWB-NB1 , by the GATEWAY system (Life Technologies, CA), and then introduced into enf2 plants. To express the VENUS-ENF2 fusion gene from the ENF2 promoter, this 6.1-kb fragment was modified by insertion of VENUS CDS between the region of the putative plastid-transit peptide (predicted by TargetP, http://www.cbs.dtu.dk/services/TargetP/) and the PotD/F homology domain. For the ene complementation experiment, a 10.5-kb AT1G80070/SUS2 genomic fragment, including 527 bp upstream from the start codon and 498 bp downstream from the stop codon, was amplified from the wild-type genome by PCR using the primers 5′-CGGGGTACCTGCCGATTCTCCCGGATTTTCA-5′ and 5′-ATGAGCTGCGGCCGCAGGAGGGATGATAAAACTGCTGT-3′. This 10.5-kb fragment was finally transferred into the vector pGWB-NB1 as the 6.1-kb ENF2 fragment above. For the construction of FILpro:CRE-GR gene, the 6,011-bp FIL promoter  and the CRE-GR coding sequence in pML518  were cloned in tandem into the multicloning site of a modified pBluescript II SK+ and finally transferred into the vector pGWB-NB1. The binary vector containing the 35Spro:loxP-Ter-loxP-VENUS gene was previously described as pML988 .
For mapping of the ENF2 and ENE loci, the F2 population from the F1 hybrid between enf2 (enf2-1 ene, Col accession background) and an L.er accession plant was used. The ENF2 locus was mapped into a 54 kb region of chromosome I using polymorphism markers. Sequencing of all of the annotated genes (AT1G31370 to AT1G31540) in this region found a mutation in the AT1G31410 gene. The ENE locus was linked to the lower arm terminus of chromosome I, and the mutation in the AT1G80070/SUS2 gene was found by sequencing the linking region. New CAPS and SSLP markers were designed using information from the Monsanto Arabidopsis Polymorphism and L. er Sequence Collection (http://www.arabidopsis.org/Cereon/index.jsp).
ENF2 mRNA Splicing Analysis
Total RNA was extracted from whole aerial parts of 8-day-old seedlings with the Plant RNeasy Mini Kit (QIAGEN, Germany). cDNA synthesis was performed using the SuperScriptIII First Strand Synthesis System for RT-PCR (Life Technologies) with a mixed primer of random hexanucleotide and Oligo(dT), and part of the ENF2 cDNA was amplified by PCR of 40 thermal cycles to saturate the amplification. The primer sequences were 5′-CCGATTGTCGTTACAGAGAATG-3′, 5′-AGGAGCTTTTTCTCCCGCATA-3′ and 5′-ACTCGTCCTCCTCTTTGTTC-3′ (blue, pink and orange arrows in Figure 5A, respectively). The PCR products were analyzed by conventional agarose gel-electrophoresis, and each fragment of a distinct size was sequenced to identify the abnormal exon junctions. The same PCR products were also applied to microfluidics-based electrophoresis, using a 2100 Bioanalyzer (Agilent Technologies, CA), to detect the normally spliced mRNAs.
qRT-PCR Analysis for the Plastid Genes
Total RNA was extracted from a shoot apex sample containing the apical meristem and only approximately the seven youngest leaves of not more than 500 µm in height. The cotyledons and hypocotyl were eliminated as much as possible. To compare the wild type, enf2, norflurazone-treated plant and enf2 gun1 at comparable stages with similar leaf numbers and sizes, their shoot apices were collected at 5.5, 7, 10 and 13 days old, respectively. cDNA synthesis was performed with QuantiTect Reverse Transcription Kit (QIAGEN) accordingly to the manufacturer's instructions. For quantitative PCR, QuantiTect SYBR Green PCR Kit (QIAGEN) and the primer sets shown in Table S1 were used. The data collection and analysis were performed with Rotor-Gene Q (QIAGEN) and the Rotor-Gene 6000 series software 1.7 (QIAGEN). Some primer sequences were based on the previous study . The average expression levels and the standard errors were calculated from biological quadruplicate data.
Mathematical Model and Simulations
All calculations and related graphical representation were performed with Mathematica 7.0 (Wolfram Research, IL). The Hill coefficients for the functions fi and gi were set to n = 2 and n = 1, respectively, because the transcriptional repression is generally implemented by dimerized or larger complexes of transcription factors and mRNA cleavage by small RNA is a one-to-one reaction, but other higher values of the Hill coefficients gave similar results (data not shown). For numerical simulations, equations (1–2) were discretized in time with the time step Δt = 0.2 by the fourth-order Runge–Kutta method, and the reflective boundary condition was imposed.
Sequence data from this article can be found in GenBank/EMBL databases under the following accession numbers: AtENF2, NP_174426.2 (Arabidopsis thaliana); NtENF2, XP_003628983.1 (Medicago truncatula); OsENF2, CAE01823.2 (Oryza sativa); ZmENF2, NP_001146059.1 (Zea mays); SmENF2, XP_002991704.1 (Selaginella moellendorffii); PpENF2, XP_001760514.1 (Physcomitrella patens); CsENF2, EIE23974.1 (Coccomyxa subellipsoidea); VcENF2, XP_002946447.1 (Volvox carteri); AvENF2, YP_323566.1 (Anabaena variabilis); NsENF2, ZP_01631537.1 (Nodularia spumigena); NaENF2, YP_003722186.1 (Nostoc azollae); BbPotD, AAB91528.1 (Borrelia burgdorferi); MhLpp38, AAA84748.1 (Mannheimia haemolytica); PfPotD, AAC15511.1 (Pseudomonas fluorescens); AaPotD, AAC27498.1 (Aggregatibacter actinomycetemcomitans); TpPotD, AAC65630.1 (Treponema pallidum); HiPot2, P44731.2 (Haemophilus influenzae); HiPot1, P45168.1 (Haemophilus influenzae); EcPotF, AAC73941.1 (Escherichia coli) and EcPotD, NP_415641.1 (Escherichia coli).
A simple mathematical model generates the shifting boundary between gene expression domains. (A) The function fj(z) is a decreasing function ranged between pj and pj + rj. (B) The function gj(z) is an increasing function ranged between dj and dj + cj. (C–F) The relationships between the parameter and the boundary dynamics type. p1 and p2 (C), r1 and r2 (D), d1 and d2 (E), c1 and c2 (F), are varied from the parameter set for Figure 1C.
The expression patterns of FILpro:GFP and 35Spro:miYFP-W in leaf primordia at various developmental stages. (A, B) The boxed region in Figure 1B. 35Spro:miYFP-W signal (A) and FILpro:GFP signal (B) are individually shown with schematic illustrations below. (C–P) The leaf primordia at around P0 stage (C, D), P1 stage (20-µm-long) (E, F), around P6 stage (G–J) and around P10 stage (1,300-µm-long) (K–P). Confocal images show FILpro:GFP (green) and 35Spro:miYFP-W (magenta) signals in longitudinal (C) and transverse (D–F, L–P) sections and the section planes parallel to the lamina (H–J). (H–J), A Z-series of optical sections with 5 µm intervals from the same primordium. (L–P), A series of transverse sections from the same primordium. The approximate heights of the observation planes from the leaf base are indicated. (G, K), The scanning electron microscope images showing the elongation of marginal tip cells at each stage. Arrowheads indicate the marginal tip cells elongating (white) and not elongated yet (black). Scale bars represent 50 µm (C), 20 µm (D–F), 100 µm (G–J, L–P) and 500 µm (K).
The efficiency and spatio-temporal specificity of the CRE/loxP recombination. (A, B) Confocal images showing the fluorescent signals from VENUS (yellow) of FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS system (A) and FILpro:GFP (green) and 35Spro:miYFP-W (magenta) (B) in a longitudinal section of a reproductive shoot apex. Arrowheads, the center of the flower primordium; “+”, the center of the shoot apical meristem; Scale bars, 50 µm. (C–F) Stereoscopic fluorescent images of the third leaves from the FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS plants grown on DEX containing (C, D) and DEX-free (E, F) medium. ad, adaxial view; ab, abaxial view; Scale bars, 1 mm. The average sizes (%) and the standard errors of the epidermal VENUS expression areas measured from more than ten of such images are shown below each image. (G, H) Confocal images showing the VENUS (yellow) expression pattern in the transverse sections of FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS plants at 6 hours (A) and 12 hours (B) after DEX application. Each differential interference contrast (DIC) image is shown on the right. “+”, the center of the shoot apical meristem; ad, adaxial side; ab, abaxial side; Scale bars, 50 µm.
The VENUS expression patterns in the FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS leaves. (A–C) Fluorescent stereoscopic images of the third leaves treated with DEX from the day 4 (A), day 6 (B) and day 8 (C). left panel, both fluorescence of VENUS (yellow-green) and chlorophyll (red); right, VENUS fluorescence alone. (D–H) Confocal images showing VENUS fluorescence (yellow) and chlorophyll fluorescence (red) in the leaf sections. The corresponding section planes are indicated in (A–C) as broken lines. Scale bars represent 1 mm (A–C), 500 µm (D–H).
The enf2 phenotypes. (A–D) Confocal images of transverse sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression in the wild type (A, B) and enf2 (C, D) leaf primordia. FIL-expression area sizes (%) are indicated at the bottom right. The specimens in (A, C) are at around the P2 stage, and those in (B, D) are at around the P4 stage of leaf development. (E) The VENUS expression area sizes (%, y-axis) in wild-type (black lines) and enf2 (red lines) leaves harboring FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS. The x-axis represents the DEX treatment dates. The data for the adaxial epidermis and the adaxial-most mesophyll are shown as broken lines and normal lines, respectively. Bars indicate the standard errors. (F–N) Stereoscopic images showing VENUS fluorescence (yellow-green) of FILpro:CRE-GR 35Spro:loxP-Ter-loxP-VENUS in the third leaves of 15-day-old enf2. The DEX treatment dates are indicated at the bottom left. (F–M) are adaxial-side views, and (N) is an abaxial-side view. (O) The needle-like structure of an enf2 leaf. n.s., not significantly different (p≥0.05, t-test) between the wild type and enf2. Scale bars represent 50 µm (A–D) and 1 mm (F–O).
The transgene ENF2 and ENF2pro:VENUS-ENF2 rescue the enf2 phenotype. The stereoscopic images of seedlings (left), confocal images showing FILpro:GFP expression patterns (middle) and the corresponding DIC images (right) are shown for each plant of the wild type (A), enf2 (B) and transformed enf2 (C, D). The transgenes ENF2 (C) and ENF2pro:VENUS-ENF2 (D) are introduced into enf2. Scale bars represent 2 mm (left panels) and 100 µm (middle and right panels).
The effects of the ene mutation. (A) The electropherogram of the ENF2 RT-PCR products (Figure 5B right panel) from the wild type, enf2-1 and enf2-1 ene (enf2) plants. Each line shows average of each triplicate data. The peaks of normally spliced ENF2 mRNA are indicated by red arrowheads and displayed in a close-up view (inset). (B–F) Seedlings of enf2-2, the wild type, enf2-1, ene and enf2-1 ene (enf2). (G) FIL-expression area sizes (%, y-axis) at different stages (grouped by section area sizes, x-axis) of wild-type, enf2-1, ene and enf2-1 ene (enf2) leaf primordia. Bars indicate the standard errors. n.s., not significantly different; *, significantly different (p<0.05, t-test) between the wild type and each mutant. (H) Schematic representation of the AT1G80070/SUS2/ENE gene. (I–L) Seedlings of the wild type (I), enf2-1 single mutant (J), enf2 mutant (K) and SUS2-transformed enf2 (L). These plants were grown under 16°C to emphasize the growth difference between enf2-1 single mutant and enf2 mutant. Scale bars represent 1 mm (B–F) and 5 mm (I–L).
Amino acid sequence alignment of ENF2 and PotD homologs. Only the part mainly interacting with polyamine in PotD is shown. Red represents the residues indispensable for the interaction with polyamine in PotD and conserved in other homologs . Green represents the ENF2 residues corresponding to the red parts and conserved among other homologs.
The plastid in enf2 shows defects in chloroplast development and plastid gene expression profile. (A–F) The transmission electron microscope images showing the plastid in the wild type (A–C) and in enf2 (D–F). All images indicate subepidermal cells. The stages are meristem (A, D), leaf primordia at the P4–P6 stage (B, E) and mature leaves (C, F). Scale bars represent 1 µm (A, B, D, E), 2 µm (C, F). (G) qRT-PCR results showing the transcript abundance of plastid genes encoding proteins (left eighty) and 16S and 23S rRNA (right two) in enf2. The results were normalized to 18S rRNA, and the enf2 values relative to the wild type are represented as log2 values. The protein-encoding genes are sorted on the x-axis by their location on the plastid genome, which corresponds to the order in Table S1. Error bars indicate standard errors.
The effects of norflurazon treatment and dark growth. (A–C) Seedlings of the norflurazon-treated plant (A) and the dark-grown plant (B, C). (B), A close-up view of a leaf lamina of dark-grown seedling. (D, E) Confocal images of transverse sections showing FILpro:GFP (green) and 35Spro:miYFP-W (magenta) marker expression in each plant of (A, B). (F) qRT-PCR results showing the transcript abundance of plastid genes encoding proteins (left eleven) and 16S and 23S rRNA (right two) in norflurazon-treated shoot apex (grey). The results were normalized to 18S rRNA, and the relative values to the untreated plants are represented as log2 values. Error bars indicate standard errors. The data of enf2 mutant (white) are the same as in Figure S9. (G) A wild-type seedling treated with 25 µM norflurazon and 450 µM lincomycin. (H) An enf2 seedling treated with 150 µM lincomycin. (I) A wild-type seedling treated with 450 µM lincomycin. Scale bars represent 1 mm (A–C, G–I) and 50 µm (D, E).
The involvement of plastid retrograde signal in the developmental effects from the plastid gene expression. (A) A gun5 seedling treated with 230 µM lincomycin (B) An enf2 gun5 seedling. (C) FIL-expression area sizes (%, y-axis) at different stages (grouped by section area sizes, x-axis) of the wild-type and gun1 leaf primordia. Bars indicate the standard errors. n.s., not significantly different (p≥0.05, t-test) between the wild type and gun1. (D) The qRT-PCR results showing the transcript abundance of plastid genes encoding proteins (left eleven) and 16S and 23S rRNA (right two) in enf2 gun1 (grey) shoot apex. The results were normalized to 18S rRNA, and the relative mutant values to the wild type are represented as log2 values. Error bars indicate standard errors. The data of enf2 mutant (white) are the same as in Figure S9. (E, F) The wild type and gun1 seedlings grown on the standard medium after growth on the lincomycin-containing medium for two weeks each. Scale bars represent 1 mm.
The model of mutual repression and mobility is applicable to simulation of phb-1d/+ phenotype. Computer simulation results with an assumed wild type parameter set (A): p1 = p2 = 0.1, r1 = 2.0, r2 = 1.8, d1 = d2 = 0.1, c1 = c2 = 2.0 and DAD = DAB = 0.1, and an assumed phb-1d/+ parameter set (B): p1 = p2 = 0.1, r1 = 2.0, r2 = 1.8, d1 = d2 = 0.1, c1 = 1.9, c2 = 2.0 and DAD = DAB = 0.1. Note that c1 value is smaller in B than in A. The adaxial-most cell was fixed in the AD-expressing state through each simulation, and the other abaxial five cells were set to be in the AB-expressing state as their initial conditions and followed the equations (1–4) during the simulations.
Primers used for plastid gene expression analyses.
We thank ABRC; the Model Plant Research Facility in National Institute for Basic Biology [NIBB] Bioresource Center; N. Mochizuki (Kyoto Univ.) for the seeds; M. Lenhard (Universität Potsdam) and T. Nakagawa (Shimane Univ.) for the vectors; M. Kanai (NIBB) for the primers; N. Sugimoto (Fujita Health Univ.) for performing the RT-PCR experiments; Y. Yagi (Kyushu Univ.), T. Shiina (Kyoto Prefectural Univ.) and members of Kiyotaka Okada's lab (NIBB) for their technical advice and helpful discussions; and T. Shikanai (Kyoto Univ.), I. Small (Univ. of Western Australia), R. Kerstetter (Monsanto Company), A. Mochizuki (RIKEN) and C. Kawaguchi (NIBB) for their helpful discussions.
Conceived and designed the experiments: TTa, KOk, RTs. Performed the experiments: TTa KWa KTo MKo KTa. Analyzed the data: TTa. Contributed reagents/materials/analysis tools: TTa KWa KTo NMa MKa MNi KOk. Wrote the paper: TTa HFu KTo KTa RTs KOk. Performed the numerical simulations: TTa HFu.
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