Roles for the C. albicans Mediator Middle domain subunit Med31 in the expression of genes related to morphogenesis, host–pathogen interactions, and pathogen-specific gene families

To start delineating the function of the Mediator complex in C. albicans, we made homozygous deletion mutants in the Middle domain subunit Med31. In C. albicans, Med31 is encoded by orf19.1429 and it displays 48.2% and 39.80% sequence identity with its S. cerevisiae and Schizo. pombe orthologs respectively. Transcriptome-wide profiles of the med31ΔΔ mutant were obtained and compared to those of a complemented med31ΔΔ+MED31 strain. Routine manipulations during mutant strain construction can result in gross chromosomal rearrangements, such as aneuploidies [41], which could profoundly affect the results of transcriptome analysis. Inspection of the med31ΔΔ transcriptional profile in the chromosomal context did not reveal a colour distribution associated with aneuploidies (Figure S1A), indicating that the med31ΔΔ mutant has the same chromosomal structure as the complemented strain. Additionally, gene sets representing 50 kb fragments were included in the Gene Set Enrichment Analysis (see below), and this analysis also did not reveal any gross chromosomal alterations (data not shown).

In agreement with a general role for Med31 in gene expression, and consistent with data from S. cerevisiae and Schizo. pombe [7], [28], [42], [43], 7.8% of the genome (510 genes) was differentially expressed in the absence of MED31 (cut-off of 1.5 fold, p<0.05, Dataset S1). Out of the genes differentially expressed in med31ΔΔ cells, 61.7% (315) were down-regulated and 38.2% (195) were up-regulated. This is consistent with a predominantly positive role of Med31 in transcription, and is in line with reports in S. cerevisiae and Schizo. pombe [7], [28], [42], [43]. To reveal the cellular pathways regulated by Med31 in C. albicans, we performed Gene Set Enrichment Analysis (GSEA) [44], [45]. GSEA compares a list from the transcript profile of interest created by ranking all of the genes according to the change in their expression (in this case that of a med31ΔΔ mutant) to a predefined gene set, and asks if a specific gene set is enriched in the top (up-regulated genes) or the bottom (down-regulated genes) of the ranked list [44], [45]. A ranked list of genes from the transcript profile of med31ΔΔ cells was compared to a custom database of 8123 gene sets (http://candida2.bri.nrc.ca/andre/GSEA/index.cfm; Sellam and Nantel, submitted) constructed using GO annotations and protein interaction data from CGD (PMID: 19808938), SGD (http://www.yeastgenome.org) and BioGRID [46], most currently published C. albicans transcriptional profiling and ChIP-CHIP experiments, our own TF motif database (PMID: 18342603), and S. cerevisiae genetic-association data (PMID: 20093466). Since profiles can exhibit correlations with hundreds of overlapping gene sets, significantly enriched gene sets (p<0.005, FDR<25%) were further organized and visualized using the Cytoscape: Enrichment Map plug-in (PMCID: PMC2981572), which produces networks of gene sets that share significant overlaps with each other (Figure 1A shows the most prominent networks of genes; the complete network is shown in Figure S2 where the details can be visualised by using the “zoom in” function in the pdf document). Figure 1B shows examples of enrichment plots for selected gene sets. The complete GSEA output can be found at http://dl.dropbox.com/u/7211133/Med31%20GSEA%20Results.zip.

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Figure 1. GSEA analysis of the genes differentially expressed in the absence of Med31 in C. albicans.

A) The network of functional groups of gene regulated by Med31 was constructed with GSEA and Enrichment Map. Blue circles are down-regulated gene sets, while the up-regulated gene sets are represented by red circles. The diameter of the circle reflects the number of modulated gene transcripts in each gene set. Where different functional groups of genes are linked in the network, they are separated by grey lines to indicate the functions. The full network is presented as a pdf document in Figure S2, where the details can be visualized using the “zoom in” function. B) Example enrichment plots for selected genes sets differentially expressed in the med31ΔΔ mutant are presented. On the x-axis are genes ranked according to their expression in the med31ΔΔ mutant, starting with the up-regulated genes on the left hand side, and all the way down to the down-regulated genes on far right. The position of the individual genes in the gene set are shown by black vertical lines. The cumulative value of the enrichment score (y-axis) is represented by the green line. A positive normalised enrichment score (NES) indicates enrichment in the up-regulated group of genes in the med31ΔΔ mutant, while a negative NES indicates prevalence of the genes in the down-regulated group. The title for each of the graphs indicates the genes set used to compare to the med31ΔΔ set. BMDM phagocytosis: the set of genes up-regulated in C. albicans upon phagocytosis by bone-marrow derived monocytes [47]; CDC5-7h-UP: gene set up-regulated in the C. albicans 7 h post depletion of the polo-like kinase CDC5 [53]; HU-6h-Down: gene set down-regulated upon a 6 h treatment of cells with the DNA replication inhibitor hydroxyurea (HU) [53]; RAS1-Hyphae-up: gene set up-regulated in ras1 mutants under hyphal growth [52]; G1-S and S-G2: genes expressed at the G1-S and S-G2 phases of the cell cycle in C. albicans [54]. p value of 0.0 represents <0.001, FDR is the false discovery rate.

https://doi.org/10.1371/journal.pgen.1002613.g001

GSEA detected enrichment for nucleolar functions, rRNA and ribosome biogenesis genes, and genes involved in nucleotide biosynthesis in the set of genes down-regulated in the med31ΔΔ mutant, while genes required for mitochondrial function were up-regulated (Figure 1A and 1B). Enrichment was also found in gene sets important for virulence-promoting function in C. albicans. Those include genes differentially expressed during C. albicans-host interactions with mouse macrophages [47], reconstituted human oral epithelial cells [48] and polymorphonuclear leukocytes [49], as well as genes differentially expressed in conditions which alter cellular morphogenesis, such as the induction of hyphal growth [50], [51], mutations in the Ras-cAMP morphogenesis pathway (ras1 and cdc35/cyc1) [52], and inhibition of cell cycle progression that causes pronounced polarised growth (treatment with hydroxyurea or down-regulation of the polo-like kinase CDC5) [53] (Figure 1A and 1B). Genes expressed at the G1-S and S-G2 transition of the cell cycle [54] were up-regulated in the med31ΔΔ mutant, as were those required for membrane and cell wall biosynthesis (Figure 1A and 1B). Genes required for cytokinesis were down-regulated (as shown by the black arrow in Figure 1A, and in the enrichment plot in Figure 1B).

Modulation of several gene sets enriched in the med31ΔΔ mutant, for example down-regulation of genes required for protein synthesis and up-regulation of those required for cell wall biogenesis, is part of a more general stress response in C. albicans [55]. Mediator has been previously implicated in stress responses in yeasts [7], [28], and it is therefore possible that some of the differences in the med31ΔΔ transcriptome are due to activation of stress responses upon loss of Med31 function. However, our analysis indicates that this is unlikely to be the cause for much of the differential gene expression in the mutant. There was little correlation between the med31ΔΔ transcriptional profile and our large database of transcriptional profiles produced from stressed cells when analysed by GSEA (of note, profile to profiles comparisons such as those done by GSEA tend to produce the strongest correlations and therefore if a correlation existed it is very likely that it would have been detected by GSEA). We further used scatter plots to directly compare the med31ΔΔ profile with stressful conditions, such as osmotic or oxidative stress, and these comparisons confirmed lack of extensive correlation between the med31ΔΔ transcriptome and differential gene expression upon stress (Figure S1B).

Analysis of gene ontology terms using the GO term finder tool at the Candida Genome database and the genes up- or down-regulated by at least 1.5 fold in med31ΔΔ cells (see Dataset S1) confirmed that genes related to morphogenesis, mitochondrial function and the cell wall were differentially expressed (Table 1 and Dataset S2). Interestingly, several central regulators of filamentous differentiation, such as the transcription factors Tec1, Efg1, Cph1 and Nrg1, were amongst the down-regulated genes, as were six out of the eight genes from the FGR6 (Filamentous Growth Regulator) family located in the RB2 repeat sequence (Dataset S2). The FGR6 family is one of the gene families found to be enriched in pathogenic yeast species, and specifically expanded in C. albicans [56]. While GSEA scored the cell wall gene set as up-regulated, we noticed that there were also several genes in this group that appeared at the bottom of the list, in the down-regulated group. In fact, another Candida-specific gene family expanded in pathogens was down-regulated in the med31ΔΔ mutant, that encoding the ALS cell wall adhesins [56] (Table 2). The major C. albicans adhesin ALS1 was one of the most down-regulated genes in the mutant (5 fold down-regulation, Table 2 and Dataset S1). ALS5 and ALS6 were also down-regulated (Table 2), but of note, these genes are expected to be expressed at very low levels in the wild type. Additionally, several other genes encoding cell wall proteins were down-regulated in the mutant, as were genes necessary for cell wall construction and remodelling, in particular those required for cytokinesis and regulated by the transcription factor Ace2 (e.g. the chitinase CHT3 and the endoglucanase ENG1) [57] (Table 2; notably GSEA also scored the cytokinesis genes as down-regulated and this is shown in Figure 1). The existence of several down-regulated cell wall genes indicates that the up-regulation of genes with roles in cell wall integrity that is detected in the med31ΔΔ mutant (Figure 1 and Table 2) likely reflects a compensatory feedback regulation due to a defective cell wall structure in the absence of Med31. That med31ΔΔ mutants have altered cell walls is supported by phenotypic analysis demonstrating changes in sensitivity to the cell wall targeting drugs congo red and calcofluor white (Table 3).

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Table 1. GO term analysis of genes differentially expressed in the C. albicans med31ΔΔ mutant.

https://doi.org/10.1371/journal.pgen.1002613.t001

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Table 2. Cell wall–related genes differentially expressed in the C. albicans med31ΔΔ mutant.

https://doi.org/10.1371/journal.pgen.1002613.t002

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Table 3. Stress responsive phenotypes of the C. albicans Mediator mutants.

https://doi.org/10.1371/journal.pgen.1002613.t003

In conclusion, the transcriptome analysis indicated a broad role for Med31 in cell physiology in C. albicans, with functions in morphogenesis and cell cycle progression, growth and metabolism, cell wall integrity, the expression of the cytokinesis genes under the control of the transcription factor Ace2 and those regulated by the interaction of C. albicans with host cells. Finally, two gene families enriched in pathogenic yeasts, the FGR family of filamentous growth regulators and the ALS adhesins, required Med31 for wild type expression levels.

Regulation of Ace2-dependent genes and cell wall adhesins by Med31 in yeast and hyphal growth

Down-regulation of Ace2 target genes in the absence of Med31 in C. albicans is in agreement with a role for Med31 and other Mediator subunits in Ace2-dependent gene expression that is conserved between C. albicans, Schizo. pombe and S. cerevisiae (this study and [28]). Comparing more broadly the genes affected in med31ΔΔ cells with those reported to be differentially expressed in the C. albicans ace2 mutant [57] revealed that differential expression of 35 genes is shared between these two transcription factors (Table S1). Genes involved in cytokinesis and cell wall functions were predominant in the shared “down-regulated” group, whereas mitochondrial biogenesis genes were predominant in the shared “up-regulated” group (Table S1). This analysis suggests that the functions of Med31 in cell wall integrity and metabolism are mediated, at least in part, by Ace2-dependent roles.

To explore this further, we used quantitative PCR (qPCR) to directly compare the expression levels of candidate genes in the med31ΔΔ and ace2ΔΔ mutants (Figure 2A). Under yeast growth conditions (as was done for the transcriptome analysis) the mRNA levels for the chitinase CHT3 and the cell wall proteins PIR1, EAP1 and ALS1 were reduced in both med31ΔΔ and ace2ΔΔ cells (albeit to a different degree). CHT3 and PIR1 have been previously reported as Ace2-targets, while ALS1 and EAP1 were not [57]. The expression of the transcription factor TYE7 was reduced in med31ΔΔ cells (consistent with the microarray data), but not in the ace2ΔΔ mutant.

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Figure 2. Med31 is required for the expression of Ace2-dependent genes and adhesins in yeast and hyphal growth.

A) Cells from med31ΔΔ or ace2ΔΔ mutants were grown in YPD at 30°C (yeast morphology) or in Spider media at 37°C to induce filamentous growth, and levels of the indicated genes determined by quantitative PCR. The expression of the indicated genes was normalised to ACT1 and expressed relative to wild type levels, which were set to 1. Equivalent results were obtained when the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) encoding gene TDH3 was used for normalization (Figure S3). Shown are the averages of at least three independent experiments and the standard error. B) The promoter regions (2 kb) of ALS1, ALS3 and EAP1 were searched for putative Ace2 binding motifs from S. cerevisiae (RRCCAGC) or C. albicans (MMCCASC) [57]. The three motifs conforming to C. albicans consensus sequences were found for the ALS3 promoter, whereas none were found for the other two genes. The activating regions A1 and A2 in the ALS3 promoter were mapped by [59]: A1: from −321 bp to −471 bp (upstream of the START codon); A2: from −1049 to −1438 bp.

https://doi.org/10.1371/journal.pgen.1002613.g002

In C. albicans, the expression of cell wall proteins is activated upon filamentous differentiation [50], [51], [58]–[61]. This includes ALS1 and other ALS and non-ALS adhesins. Therefore, we next tested if Med31 was required for the expression of the ALS1, and two hypha-specific adhesins ALS3 and HWP1, during filamentous growth. For these experiments cells were grown in filament-inducing Spider media at 37°C. The mRNA levels of both ALS1 and ALS3 were down-regulated in med31ΔΔ cells (Figure 2A). Ace2 was not required for ALS1 expression during hyphal growth, while the levels of ALS3 were lower in cells lacking Ace2, but the effect was less than in the absence of Med31 (Figure 2A). HWP1 was up-regulated in both mutants (1.8–3 fold) (Figure 2A). Collectively, the qPCR analysis suggests that Med31 and Ace2 have common, but also independent roles in the expression of the cytokinesis genes and the cell wall adhesins during yeast and hyphal growth.

Given that we found novel cell wall protein targets that require Ace2 for wild type expression (ALS1, ALS3 and EAP1), we searched the upstream regulatory regions of these genes for putative Ace2 binding sites as defined in [57]. There are three Ace2-binding motifs within 1.5 kb upstream of the start codon for ALS3 (Figure 2B). No motifs that strictly conform to the consensus sequence were found in the promoters of ALS1 and EAP1, although variant motifs could be found (data not shown). The motifs in the ALS3 promoter included one at −467 bp, which is in a region found to be essential for ALS3 activation in hyphae (the so-called A1 region) [59]. This suggests that ALS3 could be a direct target of Ace2.

Phenotypic analysis shows roles for Med31 in cytokinesis, morphogenesis, and virulence

We next performed phenotypic analysis of the C. albicans med31ΔΔ mutant to address the biological relevance of the observed changes in gene expression. Cells lacking Med31 displayed a cytokinesis defect (Figure 3A), consistent with a role in Ace2-dependent gene expression. ∼40% of cells from med31ΔΔ cultures showed a cell-chain phenotype typical of mutants that cannot undergo cytokinesis. This phenotype was observed in two independently constructed homozygous deletion mutants and was partially complemented by re-introduction of a wild type copy of MED31 into the mutant genome (Figure 3A and 3B).

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Figure 3. Med31 is required for cytokinesis and filamentous growth of C. albicans.

A) Cultures of wild type C. albicans, the med31ΔΔ mutant and the complemented med31ΔΔ+MED31 strain were grown to log phase and cells were observed by microscopy using DIC for bright field (left panel) or through the DAPI filter for calcofluor white staining (right panel). Strains lacking Med31 display a cell separation defect, forming cell chains with the cells attached at the mother-daughter junction, as judged by staining with calcofluor white. B) To determine the proportion of cell chains at least 200 cells were counted for each of the strains. Cell counts were performed after a brief 1 s sonication to disperse cell aggregates. Shown are averages of three independent experiments and the standard error. C) Wild type or mutant strains were streaked on plates containing filamentation-inducing media and incubated at 37°C for 4–5 days. The colonies were photographed using a stereo dissecting microscope. The med31ΔΔ mutant was unable to undergo filamentous differentiation on plates in all media tested. The mutant was also compromised for filamentation in liquid media (data shown in Figure S5).

https://doi.org/10.1371/journal.pgen.1002613.g003

The med31ΔΔ mutant also displayed phenotypes consistent with altered cell membrane and cell wall integrity that were suggested by transcriptome analysis. The mutant was sensitive to formamide, SDS, the sterol-binding antifungal drug nystatin, DMSO and growth at 16°C, all phenotypes consistent with defective membrane structure. The mutant was also sensitive to the cell wall-targeting drug congo red, but more resistant to the chitin-binding dye calcofluor white (Table 3 and Figure S4). Furthermore, the med31ΔΔ mutant was sensitive to oxidative and salt stress and ethanol (Table 3 and Figure S4). Some of these phenotypes are also observed in S. cerevisiae and Schizo. pombe med31 mutants, suggesting conserved roles [28], [43]. We also tested the C. albicans ace2ΔΔ mutant side by side with med31ΔΔ for tolerance to the various compounds (Table 3 and Figure S4). Resistance to calcoflour white was also observed in the ace2ΔΔ mutant (Table 3 and [62]), as was a mild sensitivity to nystatin and growth at 16°C, indicating that these phenotypes could be due to the role of Med31 in Ace2-dependent gene expression. However, the other sensitivities of the med31ΔΔ mutant were not shared by the ace2ΔΔ mutant, and are therefore unrelated to Ace2-dependent phenotypes.

The transcriptome analysis indicated a role for Med31 in cellular morphogenesis and we therefore tested whether Med31 was necessary for the yeast-to-hypha morphogenetic switch in response to a variety of inducers in vitro. The med31ΔΔ mutant was unable to produce filaments on solid Spider and M199 media, or on plates containing N-acetylglucosamine (Figure 3C). The mutant was also compromised for filamentation in liquid media, however to a lesser extent than on plates (Figure S5). med31ΔΔ cells could filament in response to serum and in M199 media in culture, but with delayed kinetics and with a proportion of cells remaining in yeast form (Figure S5). In Spider media the mutant had a more pronounced defect, and even after 7 h cells were still largely in yeast form (Figure S5). However, after prolonged incubation of 12 h, filaments were observed in this medium also (data not shown). To address whether the role for Med31 in filamentous growth would be important in a disease context, we further tested the ability of med31ΔΔ cells to filament in vivo, using the C. albicans-Caenorhabditis elegans infection model [63], [64]. This is a well-established host-pathogen system, which recapitulates key elements of disease as seen in vertebrates, most notably, the requirement for filamentous growth [63]. Only 16.5% of the worms infected with the mutant developed filaments after 3 days compared to 49% for worms infected with wild type C. albicans or the complemented strain (Figure 4A and 4B). Moreover, for those med31ΔΔ-infected worms that did develop filaments, there was a significant delay in filamentation, there were many fewer filaments per worm compared to the wild type or complemented strains, and the filaments were much shorter (Figure 4A; a similar phenotype was observed even after 7 days of infection, Figure S6). We also evaluated the pathogenic potential of the med31ΔΔ mutant in the worm. The kinetics of killing of the worm by C. albicans was delayed in worms infected with the med31ΔΔ mutant compared to those infected with the wild type (p<0.02 for all experiments performed) (Figure 4C). This result supports a role for Med31 in C. albicans virulence.

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Figure 4. The med31ΔΔ mutant is defective for filamentation and virulence in an animal host.

A) The worm C. elegans was infected by wild type C. albicans, the med31ΔΔ mutant and the med31ΔΔ+MED31 complemented strain and the appearance of penetrative filamentation was monitored daily over a period of seven days. The worms were photographed with a 40× magnification objective. B) The percentage of worm filamentation was determined after three days of infection. Shown are averages of 4 experiments and the standard deviation. The p value was <0.002 for both the comparison of the mutant with the wild type, and the mutant with the complemented strain. C) The ability of the med31ΔΔ mutant to kill worms was determined in the first 80 h post infection, when most of the worm death due to penetrative filamentation occurs. Three independent experiments were performed and equivalent results obtained. A representative experiment is shown. The med31ΔΔ mutant killed the C. elegans host with delayed kinetics compared to the wild type and the reconstituted strains (blue line- med31ΔΔ, grey line- WT, red line- med31ΔΔ+MED31). The log-rank test used for statistical analysis returned a p value of 0.0032 for the wild type versus med31ΔΔ mutant comparison, while there was no significant difference between the wild type and the med31ΔΔ+MED31 complemented strain p = 0.5572).

https://doi.org/10.1371/journal.pgen.1002613.g004

Med31 is necessary for biofilm formation, and ALS1 is a relevant gene target for this phenotype

We next sought to show that the regulation of the adhesins by Med31 was biologically relevant. To that end, we tested the ability of the med31ΔΔ mutant to form biofilms, as both ALS1 and ALS3 are key adhesins for biofilm formation by C. albicans [65]. Biofilm formation was tested in vitro, on serum-coated silicone disks as previously described [66]. In the absence of Med31, biofilms were severely compromised in both density and depth, as determined by confocal scanning laser microscopy (Figure 5A). Scanning electron microscopy (SEM) showed a similar defect (Figure S7). Quantitative analysis confirmed the phenotype observed by microscopy (Figure 5B). The med31ΔΔ mutant displayed a strong defect at the earliest time point of 90 minutes (adherence stage). The med31ΔΔ mutant grew somewhat slower than the wild type in planktonic conditions (Figures S4 and S8), however at the 90 minutes time point in the biofilm formation assay we did not observe significant cell growth for any of the strains, including the wild type (data not shown), strongly suggesting that the defect in the med31ΔΔ mutant is due to an adherence defect and not to the observed slower growth.

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Figure 5. Med31 is necessary for wild-type biofilm formation, and ALS1 is a relevant Med31 gene target for this phenotype.

A) Mature biofilms (48 h) formed on serum-coated silicone disks were stained with FUN-1 and concavalin A-Alexa488 and imaged using confocal microscopy. The 3D images were reconstructed using the Amira 5.2.1 software. Cells lacking MED31 showed a clear defect in their ability to form mature biofilms. The depths for the biofilms were as follows: WT = 54.57 µm, med31ΔΔ = 23.47 µm and med31ΔΔ+MED31 = 45 µm. A biofilm formation defect for the med31ΔΔ mutant was confirmed by scanning electron microscopy (Figure S7). B) Biofilms of the indicated strains were formed in 96 well plates and analyzed quantitatively by crystal violet staining over a 48 h time course. Shown are averages of at least three experiments performed in triplicates and the standard error. p values were <0.01.

https://doi.org/10.1371/journal.pgen.1002613.g005

Rescue by over-expression of target genes has been previously used as a strategy for identification of transcription factor targets relevant for biofilm formation (for example in the C. albicans bcr1 mutant [65]). We employed a similar strategy to test whether ALS1 is a relevant Med31 target gene for biofilm formation. The idea is that, if lower expression of ALS1 in the med31ΔΔ mutant is contributing to the biofilm defect, its ectopic expression under a constitutive promoter should, at least in part, rescue biofilm formation by med31ΔΔ cells. As shown in Figure 5B, expression of ALS1 in the med31ΔΔ mutant under the constitutive TEF1 promoter led to a substantial rescue of the biofilm defect at all time points, including the early adherence and initiation stages. Similar rescue was observed in three independent med31ΔΔ+TEF1-ALS1 clones. Expression of TEF1-ALS1 in the wild type strain did not significantly change biofilm biomass (data not shown). As an independent confirmation that the biofilm formation defect of the med31ΔΔ mutant is not due to slower growth, we tested whether introduction of the TEF1-ALS1 construct was rescuing the growth defect of the med31ΔΔ mutant. We did not observe rescue of the mutant growth defect by TEF1-ALS1 (Figure S8), although biofilm formation was rescued (Figure 5B). This confirms that the biofilm defect is due to lower adherence and not slower growth. Collectively, these results suggest that ALS1 is a biologically relevant gene target of Med31 for biofilm formation by C. albicans.

The Mediator Head domain subunit Med20 and Srb9/Med13 from the Kinase domain also contribute to morphogenesis, biofilm formation, and adhesin gene expression in C. albicans

To address more generally the roles of Mediator in C. albicans, we constructed mutants in the Med20 subunit from the Head domain (orf19.2711.1) and the Kinase domain subunit Srb9/Med13 (orf19.1452; for simplicity the mutant is indicated as srb9ΔΔ in the figures) [27]. In agreement with a general role for Mediator in adhesin gene expression, both MED20 and SRB9/MED13 were required for the expression of ALS1 and ALS3 in hyphae, and SRB9/MED13 was further required for wild type transcript levels of HWP1 (Figure 6A). Consistent with the effects on adhesins, both MED20 and SRB9/MED13 were necessary for wild type biofilm formation (Figure 6B). In regards to filamentous growth, we observed a similar trend as with the med31ΔΔ cells: filamentous growth was severely compromised in med20ΔΔ and srb9/med13ΔΔ mutants on plates (Figure 6C), while in liquid media the effects were much less pronounced (Figure 6D). In liquid media, med20ΔΔ showed a mild defect with a larger proportion of pseudohyphae (for example see the 3 and 5 h time points in serum), while srb9/med13ΔΔ behaved like the wild type (Figure 6D). To address the similarities and differences between the Mediator subunits more broadly, we analysed whether Med31-dependent genes during yeast growth were also dependent on Med20 and Srb9/Med13 for their expression. EAP1 was down-regulated in med20ΔΔ cells, ALS1 was down-regulated in both mutants, and CHT3 was only affected in srb9/med13ΔΔ cells (Figure 7A). The med20ΔΔ mutant did not display a cytokinesis defect, consistent with wild type CHT3 levels (Figure 7B). The srb9/med13ΔΔ cells were slightly elongated and a fraction (between 20–40%) formed what appeared similar to pseudohyphae, perhaps consistent with lower CHT3 levels (Figure 7B). The med20ΔΔ and srb9/med13ΔΔ mutants shared some (but not all) sensitivities to antifungal compounds and stress conditions with the med31ΔΔ strain (Table 3), and some of these sensitivities are conserved with what has been reported for the homologous mutations in model yeasts [28].

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Figure 6. The Mediator Head domain subunit MED20 and SRB9/MED13 from the Kinase domain contribute to filamentous growth and biofilm formation by C. albicans.

A) Cells from the indicated strains were grown in hyphae (Spider media) and relative mRNA levels for the indicated genes determined by qPCR. ACT1 was used for normalization. Similar results were obtained when THD3 was used as the normalization control (Figure S3). Shown are averages for three independent biological repeats and the standard error. B) Biofilm formation for the wild type, med20ΔΔ, srb9/med13ΔΔ and complemented strains was assessed in 96 well plates by crystal violet staining as in Figure 5B. Shown are averages of at least three independent biological replicates and the standard error. p values were <0.05. C) Filamentous growth on Spider plates was assessed after 4–5 days at 37°C. The med20ΔΔ and srb9/med13ΔΔ mutants were defective, and the phenotype was complemented by reintroducing MED20 or SRB9/MED13 into the respective mutant genomes. D) Overnight cultures were grown in YPD to saturation and cells diluted into pre-warmed filamentation media (YPD +10% serum or Spider media) to assess filamentous growth at 37°C. Photographs were taken after 1, 3 or 5 hours.

https://doi.org/10.1371/journal.pgen.1002613.g006

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Figure 7. Regulation of adhesins and Ace2-dependent genes by MED20 and SRB9/MED13 in C. albicans.

A) Cells from the indicated strains were grown in yeast form (YPD) and relative mRNA levels for the indicated genes determined by qPCR. ACT1 was used for normalization. Similar results were obtained when THD3 was used as the normalization control (Figure S3). Shown are averages for three independent biological repeats and the standard error. B) Yeast cell morphology of the wild type and Mediator mutants was assessed in YPD at 30°C. The scale bar is 20 µm.

https://doi.org/10.1371/journal.pgen.1002613.g007

The roles of Med31 in adhesion are different between S. cerevisiae and C. albicans

In the model yeast S. cerevisiae Mediator has been implicated in the repression of the FLO cell wall adhesins, due to the inhibitory functions of the Kinase domain subunits (including Srb9/Med13) and the Tail domain component Sin4/Med16 [25], [40]. Repression of the cell wall adhesins by the Mediator Kinase domain has also been reported in Schizo. pombe [28]. Our data in C. albicans showed that Srb9/Med13 is a positive regulator of the genes encoding adhesins (Figure 6), thus indicating that the roles of this Mediator Kinase domain subunit in adhesion are different in C. albicans. To probe this notion further, we tested the role of Med31 in adhesion in S. cerevisiae using the Σ1278b strain background, which expresses the FLO11 adhesin and is capable of adhesion-dependent phenotypes such as biofilm formation on polystyrene [67]. The wrinkled colony morphology in the med31Δ mutant was enhanced compared to the wild type, a phenotype that is indicative of higher FLO11 levels (Figure 8A). Indeed, the expression of FLO11 was significantly up-regulated in the absence of MED31 (40 fold), and consistently, the mutant was hyper-adherent in the S. cerevisiae biofilm model (Figure 8B and 8C). The levels of the other FLO family members, which are silent in the wild type, were not up-regulated under the conditions assayed (data not shown). These results are consistent with a repressive role for Med31 in the expression of FLO11 and biofilm formation in S. cerevisiae.

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Figure 8. The roles of Med31 in adhesion are different between S. cerevisiae and C. albicans.

A) Colonies of wild type S. cerevisiae Σ1278b and the med31Δ mutant were grown on YPD plates at 30°C and photographed. The flo11Δ strain was used as the negative control, to show smooth colony morphology in the absence of FLO11. B) Expression of FLO11 was tested by qPCR after 90 min in 0.2% glucose synthetic complete media, the condition used for biofilm formation in C). Levels of FLO11 were normalized to ACT1, and expressed related to the wild type, which was set to 1. Shown are averages from at least three independent biological repeats and the standard error. C) The ability of the S. cerevisiae med31Δ mutant to adhere to polystyrene was assessed in 0.2% glucose synthetic complete media as described in Materials and Methods. Quantification was performed by crystal violet staining. At least three independent cultures were used, assayed in quadruplicates. For the mutant, two independently constructed deletion strains were used and gave equivalent results. The flo11Δ mutant was assayed in parallel as a negative control and showed no adherence at any of the time points (not shown). p<0.001.

https://doi.org/10.1371/journal.pgen.1002613.g008

Overlapping and specific roles for the Mediator subunits in C. albicans

Our data in C. albicans is consistent with reports in model yeasts in that Mediator subunits show overlapping, but also some specific roles [7], [28], [43]. In S. cerevisiae and Schizo. pombe, mutants in the Head and Middle domain subunits tend to correlate in regards to gene expression and cellular phenotypes [7], [28], [43]. We also find this for the C. albicans med31ΔΔ and med20ΔΔ mutants. For example, the expression of the cell wall adhesins ALS1, ALS3, EAP1 is down-regulated in the absence of either MED31 or MED20, both mutants display biofilm defects and a stronger filamentation defect on plates than in liquid media, and they share sensitivities to compounds such as formamide, DMSO, SDS, ethanol and congo red (Table 3). However, our data also supports differences in the roles of Med20 and Med31: Med20 is not required for the expression of the Med31-regulated genes CHT3, PIR1 and TYE7, it shows a milder biofilm formation and filamentous growth defect than Med31, and there are differences in the sensitivities to some compounds between the two mutants. Shared but also distinct roles of Med20 and Med31 are supported by results in S. cerevisiae showing a 0.41 Pearson correlation coefficient between the transcript profiles form the two mutants [43]. In Schizo. pombe, in addition to Med31, Med20 and other Head domain subunits also control the expression of Ace2-dependent genes and the mutants show defects in cell separation [28]. Deletion of MED20 in C. albicans did not result in lower expression of CHT3 or a cytokinesis defect (Figure 7). Together with Med18 and the C-terminal domain of Med8, Med20 forms a structural and functional sub-complex within the Head domain, the Med8C/18/20 submodule [28], [68], [69]. Genetic analysis in Schizo. pombe supports the idea that Med18 can compensate for the loss of Med20 [28]. It is therefore possible that Med18 would need to be inactivated in C. albicans to uncover the roles of the Mediator Head domain in Ace2-dependent transcription and cytokinesis.

In contrast to the core Mediator complex which functions predominantly in transcriptional activation, the Kinase domain is mainly a repressor of transcription and the phenotypes of the Kinase mutants in model yeasts tend not to correlate with mutants in the core Mediator (for example see [7], [28]). Our data in C. albicans supports this, as the med31ΔΔ and med20ΔΔ mutants were more similar to each other in terms of gene expression and phenotypes than to the srb9/med13ΔΔ strain. However, the srb9/med13ΔΔ strain shared some phenotypes with med31ΔΔ and med20ΔΔ including the effects on ALS gene expression, biofilm defects and sensitivities to compounds that affect the cell wall, such as congo red and SDS. The Mediator kinase domain consists of four subunits: Srb8/Med12, Srb9/Med13 and the kinase-cyclin pair Srb10/Srb11. The C. albicans mutants in Srb10/Srb11 were in the Kinase collection constructed by Blankenship et al. [35]. Our srb9/med13ΔΔ mutant shares the sensitivity to oxidative stress with cells inactivated for SRB10 or SRB11 (Table 3 and [35]), however the srb10 and srb11 mutants were reported to display wild type biofilm formation [35]. The Kinase domain subunits share many functions, but can also have different roles, in particular positive functions in the transcription of some genes have been reported that are not shared by the whole domain [70], [71]. The data reported here (Figure 6) and in [35] support distinct roles for the C. albicans Mediator Kinase domain subunits in biofilm formation.

The connection between Med31 and Ace2

Our data indicates that in C. albicans the transcriptional activator Ace2 could be modulating a number of Med31-dependent effects on gene expression, in particular transcriptional activation of the cytokinesis genes, the expression of the adhesins EAP1 and ALS3 and the cell wall protein PIR1, and the regulation of genes with mitochondrial functions (Figure 2 and Table S1). The ALS3 promoter contains a putative consensus Ace2 binding site within a region known to be required for activation [59], suggesting it could be a direct target of Ace2. Consistent with shared functions, both ace2ΔΔ and med31ΔΔ mutants display a cytokinesis defect, as well as adherence and biofilm formation phenotypes (this study and [72]). In Schizo. pombe Mediator interacts directly with Ace2, via the Head domain subunit Med8, and therefore Mediator plays a direct role in Ace2-dependent transcription [73]. By analogy to Schizo. pombe, and given that we did not observe a change in transcript levels for ACE2 in the med31ΔΔ mutant that would indicate an indirect effect (Dataset S1), we propose that in C. albicans Ace2 also interacts directly with Mediator for transcriptional activation. Our data also suggests that Med31 and Ace2 have roles in transcription that are independent of each other. Unlike Ace2, which is indispensable for cytokinesis in C. albicans, a milder phenotype is observed in the absence of Med31 (Figure 3B). There is only a partial overlap between genes differentially expressed in ace2 and med31 mutants respectively ([57] and this study, Table S1), and the degree of the effects on gene transcription differs between the two mutants (for example CHT3 is much more affected by inactivation of ACE2 than MED31, while MED31 has a stronger effect on PIR1, Figure 2). ALS1, which we show is a key target of Med31, does not require Ace2 during high transcription in hyphae (Figure 2). As in Schizo. pombe [28], [73], it could be that in C. albicans Mediator subunits additional to Med31 are involved in co-activating Ace2-dependent transcription. Conversely, Med31 certainly acts through additional DNA binding transcription factors, which remain to be identified.

Mediator and the expression of cell wall adhesins in different yeast species

Med31, Med20 and Srb9/Med13 all positively regulate the expression of the ALS1 and ALS3 cell wall adhesins in C. albicans, and Srb9/Med13 is further required for wild type expression of HWP1. Consistent with the transcriptional defects, the mutants are defective for biofilm formation, an adhesin-dependent phenotype [65], . Moreover, our genetic data supports the notion that the regulation of ALS1 by Med31 is biologically relevant for biofilm development. The activators that mediate the effects of Mediator on adhesin transcription remain to be characterised. The results that Med31 is required for Ace2-dependent transcription, and that Ace2 has a role in ALS3 expression suggest a potential role for Ace2 in Med31-dependent regulation of ALS3 (Figure 2). The srb9/med13ΔΔ mutant showed parallels in regards to both morphogenesis and gene expression phenotypes with the mutant inactivated in the activator Bcr1. Both mutants are required for the expression of ALS1, ALS3 and HWP1, and, as was seen for the bcr1 mutant, the srb9/med13ΔΔ mutant is proficient for hyphal growth in liquid media, but shows biofilm defects (Figure 6 and [65], [66]).

The biochemical, genetic and gene expression data in yeast supports the notion that Mediator is universally required for RNA polymerase II-dependent transcription (reviewed in [12]), and we therefore suggest that Mediator is directly involved in the transcription of the adhesin genes. Chromatin immunoprecipitation (ChIP) experiments to address occupancy of the ALS gene promoters by Med31 remained inconclusive, as we observed variability between biological replicates, from minimal to large 20–30 fold enrichments (data not shown). We suspect that this variability could be due to the exact timing of the crosslinking of Med31 to the promoters in respect to the transcriptional activation of the ALS genes in hyphae and/or how uniformly filamentous growth/ALS gene transcription is induced in the population of cells in the culture. ChIP studies in S. cerevisiae have yielded different results between labs in regards to Mediator occupancy, from modest (albeit functionally important) enrichments at some constitutively transcribed genes [75], no enrichment of Mediator subunits on the majority of transcribed genes [76], [77], to detectable enrichment of Mediator subunits upstream of many active, as well as inactive genes, and even in the coding regions of some genes [78]. More prominent enrichment for Mediator subunits is seen on genes that are responsive to stress (e.g. heat shock, or change of carbon source from glucose to galactose) [75]–[77]. Mediator does not bind directly to DNA, which is likely to be a factor in ChIP experiments. It has also been proposed that the interactions of Mediator with promoters could be transient [77]. Moreover, different Mediator subunits can yield different fold enrichments over the background (for example see [76], [77]), and it is therefore possible that a Mediator subunit other than Med31 needs to be assayed to detect consistent Mediator occupancy on promoters in C. albicans. In addition to a direct role for Mediator in adhesin gene expression, an alternative (and not mutually exclusive) possibility is that Mediator regulates the expression of transcription factors, which then in turn regulate the adhesins. The expression of several transcription factors was lower in med31ΔΔ mutants (Table 1, Dataset S2), for example that of EFG1, TEC1 and CPH1, which have been previously shown to regulate the expression of Med31-regulated adhesins ALS1, ALS3 and EAP1 [59], [79].

In S. cerevisiae Med31 is a repressor of the adhesin FLO11 (Figure 8), suggesting different functions for this Mediator subunit in the expression of cell wall adhesion molecules and regulation of adhesion-dependent phenotypes in comparison to C. albicans. In the S. cerevisiae med31Δ mutant, the reminder of the Mediator complex is intact [43], supportive of a specific role for Med31 in the repression of the FLO11 gene that does not result simply from the loss of other repressor subunits from the Mediator complex. Previous reports in S. cerevisiae for Srb9/Med13 also show repression of the FLO adhesins [25], which is again opposite to the activating function for Srb9/Med13 in the expression of ALS1/3 and HWP1 that we observed in C. albicans. Other subunits of the Mediator Kinase domain as well as Sin4/Med16 from the Tail also inhibit expression of the S. cerevisiae FLO genes [25], [40]. In Schizo. pombe, the Mediator Kinase domain subunits Srb10 and Srb8/Med12 also repress the cell wall adhesins, despite the fact that some of the affected adhesins are not related to the FLO genes [28].

While the FLO and ALS gene are not related, there is conservation in terms of the pathways and transcription factors that regulate their expression in S. cerevisiae and C. albicans respectively. Examples include positive regulation by activators Flo8, Ste12/Cph1 and Tec1, and negative regulation by repressors Nrg1, Tup1 and Sfl1 [50], [59], [80]–[83]. Mediator interacts with gene-specific transcription factors to regulate gene expression [76], [84]. It is possible that the key transcription factor(s) through which Med31 and Srb9/Med13 act to regulate the adhesin-encoding genes differ between the two yeasts. How Mediator subunits repress the S. cerevisiae FLO genes is not well understood, but a suggested mechanism involves functional interactions with the repressors Sfl1 and Tup1 [85]. Both Sfl1 and Tup1 are required for repression of the ALS genes in C. albicans [50], [81], but the requirement for specific Mediator subunits in the repression of adhesins by these factors in C. albicans could differ from what has been suggested in S. cerevisiae. Another possibility relates to silencing mechanisms. The FLO genes in S. cerevisiae are subject to position-dependent silencing regulated by histone deacetylases ([86]; reviewed in [87]). The C. albicans adhesins are not known to be regulated by silencing. Mediator has been implicated in the regulation of chromatin structure and gene expression in regions affected by silencing, such as the sub-telomeres [12], [21]–[24]. Based on work in S. cerevisiae, it has been proposed that Mediator contributes to the establishment of a repressive chromatin structure by binding to the silenced regions and influencing the recruitment of the histone deaceytlase Sir2 and the histone acetyltransferase Sas2 and thus the acetylation status of histone H4 K16, a mark of active chromatin [24]. While this has not been tested directly, an interesting possibility is that the regulation of chromatin contributes to the repressive role of the Mediator subunits in the expression of the S. cerevisiae FLO genes. For example, the Tail subunit Sin4/Med16, which is a repressor of FLO11 and of the sub-telomeric FLO1 adhesin [40], [85], contributes to sub-telomeric silencing [24]. Med7 has also been found to affect chromatin structure, by regulating H4K16 acetylation and the presence of Sir2 and Sas2 at the sub-telomeres [24]. Med7 is closely functionally linked with Med31 - the N-terminal part of Med7 together with Med31 forms a structural and functional sub-module of Mediator, Med7N/31 [43]. It will be interesting to study whether differences in silencing mechanisms that operate on the FLO and ALS genes contribute to determining how Mediator subunits control their expression.

Yeast strains and growth conditions

The C. albicans strains used in this study are derivatives of BWP17 [88]. The med31ΔΔ, srb9ΔΔ and med20ΔΔ strains were constructed by standard methods based on PCR and homologous recombination, using ARG4 and URA3 as selective markers. The complemented strains were constructed by re-introducing a wild type copy of MED31, SRB9 or MED20 under own promoter and terminator into the HIS1 locus of the respective mutants using the integrative plasmid pDDB78. To make matched HIS1⁺ mutant strains, an empty pDDB78 vector was integrated into the genome of the respective strains. The ace2 mutant is a homozygous mutant in the BWP17 strain background and was a generous gift from Aaron Mitchell (this strain is also URA3⁺ ARG4⁺ HIS1⁺). The med31ΔΔ +TEF1-ALS1 overexpression strain was constructed using the plasmid pCJN498, as described in [65]. The S. cerevisiae med31Δ mutant was constructed in the ∑1278b strain using the KANMX4 cassette. The wt and flo11Δ mutant of Σ1278b were a generous gift from Todd Reynolds and are described in [67].

Standard growth conditions were YPD (2% glucose, 2% peptone, 1% yeast extract), at 30°C, 200 rpm. For ura⁻ strains the media was supplemented with 80 µg/ml uridine. The mutants were selected using minimal media lacking the appropriate amino acids. The TEF1-ALS1 overexpression strains have a nourseothricin resistance cassette (NAT) and were selected on 400 µg/ml NAT plates (nourseothricin was from Werner Bioagents). The S. cerevisiae mutant was selected on 200 µg/ml G418 plates.

For cell morphology analysis (Figure 3A), cells were classified as being in a chain if 3 or more cells were attached. An average of 200 cells per sample were counted, and the experiments were repeated at least with 3 independent cultures. The average and the standard error are shown in the figure. To observe the mother-bud junctions, cells were stained with calcofluor white (1 mg/ml) for 8 min in the dark, followed by washes in phosphate buffered saline (PBS). Imaging was done using an Olympus IX81 microscope with the Olympus cell∧M software, using the 100× objective with DIC or the DAPI filter for calcofluor white stained cells.

Filamentous growth was tested by dilution of cells from overnight cultures grown to OD⁶⁰⁰ = 0.1–0.2 into pre-warmed YPD+10% calf serum, Spider media (1% nutrient broth, 1% D-mannitol, 2 g K₂HPO₄), M199 or N-acetylglucosamine media (9 g NaCl, 6.7 g yeast nitrogen base and 0.56 g N-acetylglucosamine per liter) and incubated at 37°C for the times indicates in the figures. All cell imaging was done using an Olympus IX81 microscope with the Olympus cell∧M software. For testing filamentation on plates, C. albicans strains were re-streaked on plates containing filamentous-growth inducing media. Plates were incubated for up to five days at 37°C and colonies examined and photographed with a stereo dissecting microscope (Olympus SZX 16).

For analysis of sensitivities to various drugs and chemicals, ten fold serial dilutions of cultures from wild type and mutant strains were dropped on control plates, or plates containing the compounds indicated in Figure S4 and Table 3. Plates were incubated at 30°C for three days (unless growth was assessed at 37°C or 16°C), and photographed.

Biofilm assays and imaging

C. albicans biofilms were grown in 96-well microtiter plates or on silicone disks for quantitative or qualitative analysis respectively. Quantitative biofilm assays were performed as described [89], [90]. 100 ml of cultures of C. albicans wild type or mutant strains (10⁷ cells/ml in Spider media) were added to wells and incubated at 37°C with gentle shaking (75 rpm) for 90 min (adhesion phase). Non-adherent cells were discarded and 100 µl of fresh Spider media were added to each of the wells. Biofilms were allowed to develop for a future 4.5 h (6 h in total), 24 h, and 48 h, representing the early, intermediate or mature stage of biofilm development respectively. The medium was replenished after 24 h by aspiration and addition of fresh medium. Biofilm biomass was determined at the different time points using crystal violet staining. Wells containing only Spider medium with no yeast served as negative controls. For qualitative studies, biofilms were formed in vitro on serum-treated silicone disks, which is a well-established system for biofilm analysis [66]. Sterile silicone disks were pretreated with fetal bovine serum (Sigma) overnight at 37°C with gentle shaking (75 rpm). The silicone disks were then washed twice with PBS and transferred to a 12-well plate containing 2 ml of freshly prepared cell suspensions (10⁷ cells/ml in Spider media). The plate was incubated for 1.5 h at 37°C with gentle shaking (75 rpm) to allow the yeast to adhere to the disk surfaces. The silicone disks were then washed in PBS and transferred to a new 12-well plate with Spider media followed by incubation for 48 h at 37°C with shaking at 75 rpm. The established biofilms were examined with SEM or CLSM. For SEM, biofilms were fixed with glutaraldehyde (2.5%, v/v, in 0.1 M cacodylate buffer, pH 7.0) and 1% osmium tetraoxide at room temperature, and dehydrated with gradually increased ethanol (50%, 75%, 95%, 100%, and absolute 100%) and hexamethyldisilazane (HMDS) (50%, 75%, 95%, 100%, and absolute 100%). Samples were coated with gold with a Balzers SCD005 sputter coater and viewed under a Hitachi S570 scanning electron microscope. For CSLM, biofilms were stained with FUN-1 (10 µM, Molecular Probes) and Concanavalin A–Alexafluor488 conjugate (Con A, 25 µg ml⁻¹; Molecular Probes) for 45 min at 37°C. Stained biofilms were observed with a Leica SP5 CLSM, and images were captured and processed using the softwares Leica LAS AF and Amira 5.2.1. All experiments for quantitative analysis were repeated at least three times in triplicate, and qualitative assays were repeated at least 3 times. One-way ANOVA was used to compare the difference in biofilm biomass produced by different C. albicans strains. p values of <0.05 were considered to be statistically significant.

For S. cerevisiae biofilms (Figure 8), overnight cultures were growth in synthetic complete media supplemented with 2% glucose. The cells were then resuspended into 96 well polystyrene plates to an OD⁶⁰⁰ = 1.0 using synthetic 0.2% glucose media [67]. Adherence was assayed at the time points indicated in the figure using crystal violet staining as described above.

RNA extraction, quantitative PCR, and microarray analysis

RNA was extracted using the hot-phenol method from cultures grown in YPD at 30°C to log phase (OD⁶⁰⁰ = 1). Following hot-phenol extraction, 100 µg of RNA samples were further purified using the RNAeasy kit and following the manufacturer's instructions. The microarray analysis was performed as described [91], on microarrays spotted with 6459 70-mer oligonucleotides (GEO Platform GPL9818). Data normalization and analysis was conducted in GeneSpring GX version 7.3 (Agilent Technologies). The microarray data set has been deposited in GEO, under accession number GSE31632. Genes that were up- or down-regulated in the med31ΔΔ mutant by 1.5 fold or more (p≤0.05) were selected from a Volcano Plot and considered to be differentially expressed. Gene ontology analysis was performed at the Candida genome database (CGD, candidagenome.org) [92]. GSEA analysis ([93] and Sellam et al, submitted) was performed using the GseaPreranked tool and the weighted enrichment statistics on 6387 (for C. albicans) gene sets each containing 5–500 genes. Statistical significance was estimated from 1000 permutations. Enrichment maps were constructed with Cytoscape 2.8 (http://www.cytoscape.org; [94]) and the Enrichment Map 1.1 plug-in (http://baderlab.org/Software/EnrichmentMap) using the default settings. For quantitative PCR, reverse transcription was performed using the Transcriptor High Fidelity cDNA synthesis kit from Roche. qPCR reactions were prepared using Fast-Start Sybr Green Master (Roche) on an Eppendorf Realplex master cycler and analysed by absolute quantification. The expression levels of the mRNAs were normalized to the level of ACT1 or the GAPDH encoding gene TDH3. Three independent cultures were analyzed, with two technical replicates each. qPCR primers that enable differential amplification of the ALS1 and ALS3 genes were from Green et al [95]. Sequences for all qPCR primers used in this study are listed in Table S2. For analysis of gene expression in YPD, cultures were grown under the same condition as for the microarrays analysis, to mid log phase (OD⁶⁰⁰ = 1). For assaying expression of genes under hyphal growth, strains were grown in Spider media as described in [65].

Worm infection assays

The worm-C. albicans infection assay was performed as described previously [64]. Briefly, young adult nematodes were allowed to feed for 4 h on lawns of C. albicans grown on solid BHI media (Difco) containing ampicillin (100 µg/ml), kanamycin (50 µg/ml) and streptomycin (200 µg/ml). Worms were washed with M9 media and transferred into wells of a six-well microtiter dish (Corning) containing 2 ml of liquid media (80% M9 and 20% BHI) at 60 to 80 worms per well. The plates were incubated at 25°C, and worms were qualitatively assessed at 24 h intervals for penetrative C. albicans filamentation using a DIC microscope and photographed using an Olympus IX81 microscope with the Olympus cell∧M software. The % of worms with penetrative filamentation was determined from four independent experiments at day 3 of the infection. Means and the standard error were calculated, and the p value was determined using the student t-test. The killing assays (Figure 4C) were performed three times and equivalent results were obtained.