• Loading metrics

Multipotent Genetic Suppression of Retrotransposon-Induced Mutations by Nxf1 through Fine-Tuning of Alternative Splicing

  • Dorothy Concepcion,

    Affiliations Department of Medicine, University of California San Diego School of Medicine, La Jolla, California, United States of America, Department of Cellular and Molecular Medicine, University of California San Diego School of Medicine, La Jolla, California, United States of America, Rebecca and John Moores UCSD Cancer Center, University of California San Diego School of Medicine, La Jolla, California, United States of America

  • Lisbeth Flores-García,

    Affiliations Department of Medicine, University of California San Diego School of Medicine, La Jolla, California, United States of America, Department of Cellular and Molecular Medicine, University of California San Diego School of Medicine, La Jolla, California, United States of America, Rebecca and John Moores UCSD Cancer Center, University of California San Diego School of Medicine, La Jolla, California, United States of America

  • Bruce A. Hamilton

    Affiliations Department of Medicine, University of California San Diego School of Medicine, La Jolla, California, United States of America, Department of Cellular and Molecular Medicine, University of California San Diego School of Medicine, La Jolla, California, United States of America, Rebecca and John Moores UCSD Cancer Center, University of California San Diego School of Medicine, La Jolla, California, United States of America

Multipotent Genetic Suppression of Retrotransposon-Induced Mutations by Nxf1 through Fine-Tuning of Alternative Splicing

  • Dorothy Concepcion, 
  • Lisbeth Flores-García, 
  • Bruce A. Hamilton


Cellular gene expression machinery has coevolved with molecular parasites, such as viruses and transposons, which rely on host cells for their expression and reproduction. We previously reported that a wild-derived allele of mouse Nxf1 (Tap), a key component of the host mRNA nuclear export machinery, suppresses two endogenous retrovirus-induced mutations and shows suggestive evidence of positive selection. Here we show that Nxf1CAST suppresses a specific and frequent class of intracisternal A particle (IAP)-induced mutations, including Ap3d1mh2J, a model for Hermansky-Pudlak syndrome, and Atcayhes, an orthologous gene model for Cayman ataxia, among others. The molecular phenotype of suppression includes ∼two-fold increase in the level of correctly-spliced mRNA and a decrease in mutant-specific, alternatively-processed RNA accumulating from the inserted allele. Insertional mutations involving ETn and LINE elements are not suppressed, demonstrating a high degree of specificity to this suppression mechanism. These results implicate Nxf1 in some instances of pre-mRNA processing, demonstrate the useful range of Nxf1CAST alleles for manipulating existing mouse models of disease, and specifically imply a low functional threshold for therapeutic benefit in Cayman ataxia.

Author Summary

Retroviruses and transposable elements are molecular parasites that integrate into the host genome and require host cell machinery for gene expression, replication and dissemination. Integrating elements can alter the expression of nearby host genes through both transcriptional and post-transcriptional mechanisms. Components of the host cell machinery that can adapt to favor genetic programs of the host cell over those of the parasite may afford one level of innate immunity. In laboratory mice, endogenous retroviruses are virus-derived mobile elements that account for many spontaneous mutations. A frequent class involves retrotransposition into introns of genes in the transcriptional sense orientation, which alters host gene pre-mRNA splicing. Here we show that for the intracisternal A particle (IAP) family of endogenous retroviruses, an allele of the canonical mRNA export factor Nxf1 found in wild Asiatic mice (Mus musculus castaneus) suppresses most insertions of this class (six of seven tested). To our knowledge, these results make Nxf1 the most broadly interacting modifier gene yet documented in this well-studied species. These results have significant implications for manipulating gene expression in mouse models of disease, the role of Nxf1 in pre-mRNA processing and in the dynamic range for therapeutic intervention in Cayman ataxia.


Retroviruses and transposable elements both utilize host cell factors for their own expression and influence the expression of adjacent host genes through a variety of mechanisms. Components of host cell gene regulatory machinery that interact with molecular parasites may be regarded as components of innate immunity if they can discriminate between host and parasite expression [1]. The generality and exploitability of any given mechanism is an important practical question. Nuclear-cytoplasmic export of RNA is an important point of contact between molecular parasites and host genomes that may fit this criterion for several molecular parasites in mice and humans [2],[3]. We have previously reported that a wild-derived allele of Nxf1, which encodes the major mRNA nuclear export factor, can significantly suppress two mutations caused by insertions of endogenous retroviruses into introns of cellular genes by modulating their mature transcript levels ∼2 fold [4]. A 16 kb transgene containing the full Nxf1 haplotype, but no other recognized gene, was able to confer the modifier phenotype. Whether this interaction could be generalized to a broader class of insertional events, and if so for what range of insertions, was limited by the relatively small number of events examined.

Nxf1 (also called Tap) was first described as a cellular factor that interacts with the Tip protein of herpesvirus saimiri [5] and subsequently shown to be an essential host factor for nuclear export of unspliced viral genomes of simple retroviruses [6]. Although recruitment of Nxf1 to cellular mRNPs may generally be mediated by protein contacts [7],[8], both Nxf1 and its yeast homolog Mex67p also bind RNA directly [9][11]. In mammals, known direct targets of Nxf1 include both exogenous and endogenous viral RNAs as well as host sequences [6], [12][14]. In addition, we previously reported that one Nxf1 haplotype shows hallmarks of recent positive selection in wild Mus musculus castaneus accessions [4], which may suggest a host-pathogen interaction mediated by Nxf1 in wild populations.

Endogenous retroviruses (ERVs) are non-infectious molecular parasites that are frequent mutagens in mice. Several families of ERV are highly polymorphic among classical inbred strains and among wild accessions [15]. In laboratory mice, ERV insertions account for 10–15% of spontaneous mutations [16],[17], depending on the strains from which estimates are drawn. The intracisternal A particle (IAP) and MusD/early transposon (ETn) families of ERV, which account for most of these, have different apparent rates of transposition in different inbred strains: IAPs appear to be particularly active in C3H strains and ETn elements in A strains [16]. Characteristics of autonomously active copies have been described [18]. Interestingly, the size distribution for newly integrated ETn elements is both broader and, on average, a lower percentage of full length than for IAP elements [19]. As both families are thought to have derived originally from infectious viruses, mechanisms that regulate ERVs or mitigate their impact on host genomes may have broader implications for both gene expression and host-parasite interactions.

To test the range of insertion events for which the modifier activity of Nxf1CAST is effective, we examined gene expression, visible phenotypes, or both for five additional IAP, one LINE, and seven ETn insertion alleles. The host genes cover a wide range of phenotypes, expression patterns, and biochemical pathways:

  • The genes mutated in classical coat color mutations mahogany (Atrn) and mahoganoid (Mgrn) both mediate intercellular signaling by secreted agouti protein. Atrn encodes a transmembrane accessory receptor [20],[21], while Mgrn encodes an E3 ubiquitin-protein ligase that participates in endosomal trafficking [22]. Spontaneous alleles at either gene range in effect from modest coat color changes to spongiform neurodegeneration with associated neurological deficits [20],[21],[23],[24]. Among these alleles, AtrnmgL and Mgrnmd are de novo IAP insertions into introns in the transcriptional sense orientation [24],[25] that decrease the steady-state level of correctly processed mRNA in mutant tissues, resulting in moderate coat color darkening, but lacking the neurodegeneration seen in stronger alleles.
  • Spontaneous mocha alleles of the intracellular trafficking adapter protein gene Ap3d1 [26] include a hypomorphic IAP insertion allele (mh2J) that reduces levels of wild-type RNA and protein. In addition to coat color dilution caused by sorting defects in melanosomes, mh2J and more severe alleles show substantial mortality, neurological and behavioral impairments [27]. Because mutations in other Ap3 complex proteins are associated with Hermansky-Pudlak syndrome, mocha mice have been used to model this disease [26],[27].
  • The ataxiaJ mutation is an IAP insertion into an intron of the ubiquitin specific protease gene Usp14. Although the protein targets have not been systematically identified, loss of Usp14 activity results in synaptic defects that manifest behaviorally as tremor and ataxic gait in Usp14axJ mice [28].
  • The classical mouse locus jittery is orthologous to the CRAL-TRIO domain gene ATCAY mutated in human Cayman ataxia [29]. Patients with this recessive disorder have a prominent but non-progressive psychomotor impairment consistent with cerebellar disease [30]. The hesitant mutation (Atcayhes ) is an IAP insertion into the first intron, resulting in profound locomotor deficits with no obvious neuroanatomical correlates [29].
  • Mutations of the Mitf transcription factor gene block melanocyte development, causing white-spotting and other defects in mice and Waardenburg syndrome in humans. The mouse black-eyed white allele is a L1 LINE element inserted into an intron that disrupts splicing of one alternative 5′ exon [31]. Loss of this isoform results in recessive severe white spotting, such that the fur is most often completely white, with pigmented patches occurring in some animals. Weaker alleles of Mitf show larger and more frequent area of pigmented fur, providing a sensitive phenotypic readout for allele strength and modifier genes [32].
  • The MusD/ETn family are endogenous retroviruses that are more closely related to the IAP superfamily than most other currently active mouse retroelements [33],[34]. BALB/cJ and A/J strains carry several recent MusD/ETn family insertions that are mutagenic with respect to host genes [19]. In particular, Zhx2 is a transcriptional repressor required to down-regulate expression of Afp fetal globin RNA. Loss of Zhx2 expression in BALB/cJ (but not other BALB/c lines) due to an ETn-II insertion results in persistent Afp expression into adulthood [35],[36]. Insertion of an ETn in the gene encodng dysferlin, Dysf, in A/J mice results in loss of expression and creates an orthologous gene model for human limb-girdle muscular dystrophy 2B [37].

Here we show that Nxf1CAST suppresses six of six IAP insertions of the IΔ1 class [38], the most frequent class of new insertions, but does not suppress a full-length IAP, a L1-LINE, nor any of six ETn insertion mutations. We quantify RNA and protein levels to show a consistent ∼2-fold increase in normal gene expression from the mutant allele in each case of suppression. Concomitant decrease in the expression of mutant-specific RNAs implicates Nxf1 in pre-mRNA processing in addition to its known role in mRNA export. For disease models and other mouse mutations induced by IAP-IΔ1 retrotransposition, Nxf1CAST provides a genetic rheostat for gene activity in situ.


Nxf1CAST Suppresses RNA Expression Phenotypes of Mgrnmd, but Not AtrnmgL

To test whether Nxf1CAST can suppress the RNA processing defects in AtrnmgL and Mgrnmd, we examined whole brain RNA of progeny from genetic crosses to Nxf1CAST, comparing homozygous mutant littermates that differ in Nxf1 genotype. Because each of these crosses also segregated other loci contributing to coat color, we did not assess pigmentation phenotypes for these two mutants.

For Atrn (Figure 1), abnormally processed message from mgL alleles are detected on Northern blots by probes containing exons 5′ to the insertion site, but not by the 3′ untranslated region ([25] and Figure 1A, B). Because the large but low-abundance normally spliced message was difficult to quantify reliably from Northern blots, we used TaqMan quantitative RT-PCR to assay RNA abundance in mgL mutant brains. Comparing mgL to control animals shows non-significant reduction in abundance of 5′ sequences (Figure 1C), but ∼6-fold loss of full-length transcript, represented by an assay 3′ to the mgL insertion (Figure 1D). However, this assay shows no effect of Nxf1 genotype on Atrn expression.

Figure 1. Nxf1CAST does not suppress mRNA deficit in AtrnmgL.

(A) Scale diagram of the mouse Atrn locus indicates the location and approximate size of the IAP insertion in mgL allele and the Northern blot probe. (B) Northern blot analysis of brain poly(A)+ RNA (5 µg per lane) from non-mutant and mutant littermates shows reduced level of full-length Atrn mRNA in mgL animals, independent of Nxf1 allele (B for C57BL/6, C for CAST/Ei). (C, D) Quantitative PCR (TaqMan) data shows nominal reduction in the abundance of spliced 5′ sequences, but ∼6-fold reduction of 3′ sequences, consistent with alternative splicing and 3′ end formation in the insertion, with no difference between Nxf1 genotypes. Error bars indicate standard deviation.

In contrast, for Mgrn, Nxf1-dependent differences in the level of correctly and alternatively spliced RNA isoforms from md alleles were readily quantified (Figure 2). A probe 5′ to the md insertion (Figure 2A) detects both normal and mutant-specific Mgrn RNAs (Figure 2B). Correctly processed normal RNA is elevated in the presence of Nxf1CAST, while levels of several mutant-specific transcripts is decreased (Figure 2B–D), consistent with the mode of suppression previously reported for Pitpnvb and Eya1BOR. A probe 3′ to the insertion detects only the correctly spliced form, at levels comparable to the 5′ probe (not shown). Quantitative RT-PCR across the inserted intron confirms a ∼2-fold increase in correctly-spliced transcript levels by Nxf1CAST (Figure 2E).

Figure 2. Nxf1CAST suppresses pre-mRNA processing defects in Mgrnmd.

(A) Diagram of the Mgrn locus indicates locations and sizes of the md IAP insertion and probes. (B) Northern blot of brain poly(A)+ RNA (3 µg per lane) shows increased level of full-length Mgrn RNA and concomitantly reduced levels of mutant-specific transcripts in md mutant mice in the presence of Nxf1CAST. (C, D) Quantification of multiple independent Northern blot experiments is shown. Graphs show means of replicate experiments normalized to nonmutant control samples on each blot. Error bars indicate standard deviation. Presence of the lowest molecular weight band was inconsistent across experiments and not included in panel D. *p≤0.05, Wilcoxon signed-ranks test applied to paired (same-blot) samples. (E) Quantitative RT-PCR across the inserted intron indicates ∼2-fold increase in correctly spliced Mgrn1 RNA in the presence of Nxf1CAST. **p≤0.005, unpaired t-test with one tail.

Nxf1CAST Suppresses RNA, Protein, and Phenotypic Expression of Ap3d1mh2J

To test Nxf1CAST activity on a mutation for which protein level and phenotype were accessible, we analyzed RNA and protein levels, coat color (eumelanin) dilution and tremor severity of Ap3d1mh2J mutant animals (Figure 3). Locations of the mh2J insertion and probes are indicated in Figure 3A. Although Northern blots show high variance between experiments, comparisons between paired subjects examined on each blot shows a statistically significant increase in normal-sized Ap3d1 transcript and a modest decrease in mutant-specific transcript in the presence of Nxf1CAST (Figure 3B–D). Quantitative RT-PCR confirms the increase in correctly spliced RNA (Figure 3E). Western blots show a corresponding increase in full-length Ap3d1 protein levels detected by an antibody to N-terminal residues (Figure 3F,G). Correspondingly, a smaller protein species detected only in mutant animals is decreased in Nxf1CAST animals. As predicted from this molecular analysis, Ap3d1mh2J mutant animals also had improved pigmentation and neurological assessment scores in the presence of Nxf1CAST as rated by observers blinded to genotype (Figure 3H–J).

Figure 3. Nxf1CAST suppresses Ap3d1mh2J RNA, protein and visible phenotypes.

(A) Diagram of Ap3d1 shows location and size of the mh2J IAP insertion and Northern blot probes. (B) Northern blot of brain poly(A)+ RNA (5 µg per lane) shows that the level of full-length Ap3d1 RNA in mh2J mice is partially restored and levels of mutant-specific RNAs are reduced in the presence of Nxf1CAST. (C) Quantification of Northern blot experiments with paired samples, showing means and standard deviation of normalized values across all experiments. *p≤0.05, Wilcoxon signed-ranks test using 5 pairs (using one Nxf1 heterozygous sample to complete a pair) and p≤0.01, paired t-test with 4 pairs; each test has one tail. (D) Levels of mutant-specific RNAs appear decreased in the presence of Nxf1CAST, *p≤0.05, Wilcoxon signed-ranks test. (E) Quantitative RT-PCR analysis with primers spanning the inserted intron. **p = 0.0003, unpaired t-test with one tail. (F) Western blot of brain protein extracts shows increased level of Ap3d protein from mh2J in Nxf1CAST mice. (G) Quantification of replicate Western blots shows ∼2-fold increase in Ap3d with Nxf1CAST. *p = 0.03, paired t-test, one tail. (H) mh2J coat color dilution is attenuated in Nxf1CAST. (I) Average coat color scores, comparing mice to a printed grading matrix, and (J) tremor severity scores were assessed by observers blinded to genotype. Error bars indicate standard deviation. **p≤0.01, *p≤0.05, t-test with one tail.

Nxf1CAST Suppresses RNA, Protein, and Phenotypic Expression of Usp14axJ

We similarly tested Nxf1CAST activity on molecular and visible phenotypes of Usp14axJ (Figure 4). The insertion and probes used are indicated in Figure 4A. Quantification of Northern blots and RT-PCR experiments from brain RNA shows significantly increased levels of correctly processed RNA in the presence of Nxf1CAST (Figure 4B–D). Quantification of Western blots shows that this is translated into an increased level of Usp14 protein (Figure 4E,F). Behaviorally, Usp14axJ mutant animals also showed improved neurological assessment scores, with visibly reduced tremor amplitude in the presence of Nxf1CAST (Figure 4G and Videos S1 and S2). In contrast to other mutations suppressed by Nxf1CAST, normalized levels of mutant-specific isoforms of Usp14 RNA did not differ significantly by Nxf1 genotype. Comparing Northern blots hybridized with either 5′ or 3′ probes (as indicated in Figures 25), we find Usp14axJ and Eya1BOR differ from other suppressed mutations in producing RNA isoforms that contain 5′ exons, IAP sequences and 3′ exons [4],[28] where most others produce primarily 5′ exons and terminal IAP sequences.

Figure 4. Nxf1CAST suppresses Usp14axJ expression and behavioral phenotypes.

(A) Diagram of Usp14 locus shows locations of the axJ IAP insertion and Northern blot probes. (B) Northern blot of brain poly(A)+ RNA (4 µg per lane) shows that the level of full-length Usp14 RNA in axJ mice is partially restored and levels of mutant-specific RNAs are reduced in the presence of Nxf1CAST. (C) Quantification of paired samples from multiple Northern blot experiments, showing means and standard deviations. *p≤0.05, Wilcoxon signed-ranks test. (D) Quantitative RT-PCR shows ∼2-fold difference in expression of correctly spliced Usp14 RNA in mutant brains homozygous for Nxf1CAST. *p≤0.05, unpaired t-test with one tail. (E) Western blot of brain protein extracts shows increased level of Usp14 protein from axJ in Nxf1CAST mice. *p≤0.05, Wilcoxon signed-ranks. (F) Quantification of replicate Western blots shows increased Usp14 expression in the presence of Nxf1CAST. *p≤0.05, Wilcoxon signed-ranks. (G) Neurological assessment scores for tremor assigned by observers blinded to genotype show highly significant improvement in animals homozygous for Nxf1CAST. Error bars indicate standard deviation. **p<0.01 unpaired t-test. See Videos S1 and S2.

Figure 5. Nxf1CAST suppresses Atcayhes RNA, protein and behavioral phenotypes.

(A) Diagram of Atcay shows location of the hes IAP insertion, Northern blot probes and TaqMan assay. (B) Northern blot of brain poly(A)+ RNA (8 µg per lane) shows that level of mutant-specific Atcay transcripts in hes mice are reduced in the presence of Nxf1CAST. (C) Quantification of replicate Northern blot experiments shows reduced level of mutant-specific transcripts in Nxf1CAST brains. **p<0.01 paired t-test with one tail. (D) Quantitative PCR (TaqMan) analysis of Atcay RNA in hes homozygotes shows ∼2-fold increase in Nxf1CAST. *p<0.05 paired t-test with one tail. (E) Western blot of brain protein extracts shows increased level of Caytaxin protein from expression of Atcay in Nxf1CAST mice. (F) Quantification of replicate Western blots shows ∼2-fold increase in Caytaxin with Nxf1CAST. *p<0.05, t-test, one tail. (G) Average neurological assessment scores assigned by observers blinded to genotype show highly significant improvement in animals homozygous for Nxf1CAST. Error bars indicate standard deviation. **p<0.01, t-test, one tail. See Videos S3 and S4.

Nxf1CAST Suppression of Atcayhes Indicates High Sensitivity of Phenotype to Increased Expression

To test Nxf1CAST activity in the context of a human disease model, we analyzed several levels of molecular and behavioral phenotypes for the Atcayhes mutation (Figure 5). The locations of the hes insertion and probes are indicated in Figure 5A. Atcayhes alleles express prominent mutant-specific Atcay RNAs and very low levels of correctly processed full-length RNA [29]. Northern blots to quantify size-specific RNA levels show reduced level of each mutant-specific RNA detected by a probe 5′ to the insertion (Figure 5B,C). A probe 3′ to the insertion detects only the full length “normal” RNA and is quantifiable only in non-mutant samples (not shown). To measure levels of normal RNA in mutant samples, we used a quantitative RT-PCR (TaqMan) assay (Figure 5D). The presence of Nxf1CAST significantly increases the level of correctly processed Atcay RNA accumulating from hes alleles. This difference is also translated into higher levels of the encoded Caytaxin/BNIP-H protein (Figure 5E,F). Atcayhes mutant animals have profound ataxia and an unusual jumping behavior (see Video S3). Nxf1 genotype had a highly significant impact on Atcayhes neurological phenotypes as rated by multiple observers blinded to genotype, including both reduced ataxia and complete elimination of jumps from open field behavior (Figure 5G and Videos S3 and S4).

Nxf1CAST Does Not Suppress L1-LINE Mutation of Mitfmi-bw

To test a non-viral class of retrotransposon, we examined whether Nxf1CAST would suppress the black-eyed white L1-LINE insertion allele of Mitf. This mutation results in loss of pigmented melanocytes and extreme white spotting, leaving only occasional patches of pigment on the head or ears. Despite this low threshold for phenotype modulation, and known effects of other strain backgrounds, we saw no evidence for modification by Nxf1CAST in an F2 cross. Among 14 Mitfmi-bw, Nxf1B6 and 9 Mitfmi-bw, Nxf1CAST doubly homozygous progeny, we observed a single animal of each genotype with dark patches on the head or ears.

Nxf1CAST Does Not Suppress Typical ETn Insertions

We tested Nxf1CAST activity on both sense and antisense-oriented ETn insertions of recent origin in both BALB/cJ and A/J mice. Expression levels of Zhx2 and its repression target Afp were assayed by quantitative RT-PCR from adult liver at P40 from 24 BALB/cJ x B6-Nxf1CAST F2 animals selected by genotype (Figure 6A,B). The BALB/cJ-derived insertion allele expressed ∼1.5% non-mutant levels of Zhx2, with no difference between Nxf1 alleles. Similarly, the effect on Afp persistence, potentially a more sensitive indicator of Zhx2 function, showed no significant difference between Nxf1 alleles, although inter-individual variation was high (Figure 6B, right panel), likely due to other factors segregating in this cross [39].

Figure 6. Nxf1CAST does not suppress ETn-induced Zhx2Afr mutation.

(A) Diagram shows genomic organization of Zhx2, including location of the ETn-IIa insertion. Insertion is not to scale. (B) Quantitative RT-PCR shows reduced Zhx2 expression from mutant alleles (m), but no suppression by Nxf1CAST. Persistent Afp expression in adult liver in mutant animals is highly variable among F2 animals at P40, but not significantly different between Nxf1 genotypes. Error bars indicate standard deviation.

We tested the ability of Nxf1 to elevate transcript levels for another 5 sense and 3 antisense intronic ETn insertions in a second cross, A/J x B6-Nxf1CAST (Figure 7). Genomic organization and the location and orientation of the insertions are indicated (Figure 7A). Quantitative RT-PCR measurements from brain or muscle (depending on known pattern of expression for each gene) showed no significant differences between Nxf1 genotypes for either sense or antisense insertions (Figure 7B,C). A fifth sense-oriented insertion, in Prkca, showed no difference between inserted and uninserted alleles for either RNA or protein levels in this cross.

Figure 7. Nxf1CAST does not suppress ETn-induced mutations in A/J.

(A) Genomic organization of five sense and three antisense-oriented ETn insertions monitored in F2 mice from A/J x B6–Nxf1CAST. Insertions are not to scale. (B) Quantitative PCR (SYBR green) on brain and/or muscle cDNA shows reduced expression of sense-oriented ETn alleles, but no significant differences between Nxf1 genotypes. m, insertion allele at each indicated locus. (C) Quantitative PCR shows modest (Atp9a) or no difference (Art3, Mark3) in expression of antisense-oriented ETn insertions, with no difference attributable to Nxf1. Error bars indicate standard deviation.

Nxf1CAST-Sensitive Insertions Carry the IΔ1 Deletion

Among sense-oriented IAP elements, only AtrnmgL was not suppressed by Nxf1CAST; as the inserted intron does not appear to be differentiated in position, length, or sequence composition from mutations that were suppressed (Figures 15 and data not shown) we determined the DNA sequence of each of these inserted elements, as well as the original Pitpnvb insertion [4],[40]. We amplified each insertion using high-fidelity PCR optimized for long sequences, using unique primers flanking each insertion site (Supplemental material online). Ap3d1mh2J, Atcayhes, Mgrn1md, Pitpnvb and Usp14axJ insertions all amplified fragments of 5.5 to 6.0 kb, while the AtrnmgL insertion required modified conditions to support adequate amplification of a unique ∼8 kb product. DNA sequence analysis showed that the AtrnmgL element is a full length (type I) IAP, while each of Nxf1CAST-sensitive elements includes the 1.9 kb deletion of gag-pol sequence typical of type IΔ1 elements [38] (Figure 8A). All 6 elements belong to the IAPEz subfamily (, and contain an RTE-D transport element [41],[42] near the 3′ LTR. Calculated trees for each segment of aligned sequence shows that the full length AtrnmgL element is not otherwise an outlier in overall sequence composition, except for the undeleted region of the gag gene (Figure 8B). Inclusion in the tree of two recently identified IAP-IΔ1 insertions, Atp2b2jog and Gria4spkw1 [43],[44], suggests that they too should be sensitive to Nxf1CAST-mediated suppression as they fall within sequence clades of suppressed elements for each segment.

Figure 8. The gag region and IΔ1 deletion differentiate AtrnmgL from other IAP insertions.

(A) Organization and pairwise percent identity of sequenced IAP elements from mutations in this study. RTE, RNA transport element; ppt, polypurine tract. Sequences have been deposited in GenBank (Accession numbers FJ854355–FJ854360). (B) Neighbor-joining trees for each of four aligned sequence blocks show similar topology for LTR and pol/RTE/ppt regions. For the undeleted portion of the gag gene, AtrnmgL clusters with Pitpnvb as a separate group. Recently described and sequenced IAP-IΔ1 elements inserted in Atp2b2jog and Gria4spkw1 mutations fall within the group of suppressed elements in all four sequence regions.


Our results show that Nxf1CAST suppresses a broad and frequent class of IAP-induced mutations. The magnitude of increased normal transcript is ∼2-fold and the impact on gene expression and behavioral phenotypes are significant in each case of this class examined. Nxf1CAST increases the steady-state level of correctly spliced host gene transcript and almost always decreases the level of mutant-specific alternatively spliced transcript for six of seven sense-oriented IAP insertions examined to date (Table 1). The one exception, AtrnmgL, differs from all of the suppressed elements we sequenced in having an intact gag-prt-pol coding sequence. Sequences within the deleted region may therefore mediate an additional level of Atrn repression that is not relieved by Nxf1CAST. Each insertion, including AtrnmgL also had a number of more subtle sequence variations, including smaller indels and further studies will be required to clarify which sequence differences contribute to the lack of suppression. However, the current data do provide a clear guide for the class of insertional mutation most likely to be quantitatively modulated by Nxf1CAST, type IΔ1 IAPs, which are by far the most frequent class recovered from spontaneous mouse mutations. While it is possible that other genes within the congenic interval contribute to any one effect, transgenic mouse and lentiviral gene transfer studies with Pitpnvb indicate that the main effect is due to Nxf1, as do the consistency of findings across all six suppressed mutations. Negative data from six ETn-inserted loci indicate that Nxf1CAST is highly selective, and therefore unlikely to result in collateral changes in gene expression when used to manipulate IAP-induced mutations. Indeed, preliminary microarray data failed to identify any significant expression changes in whole brain RNA (B.A.H., unpublished data).

The simplest explanation for the molecular data from the six mutations suppressed by Nxf1CAST would be for Nxf1 to participate in pre-mRNA processing prior to the completion of splicing. This could occur by recruitment of Nxf1 to the nascent transcript by sequences in the IAP (or proteins bound to them co-transcriptionally) and subsequent interactions between Nxf1 and other components of the mRNP. Under such a model, amino acid differences (S48P and E610G) between the allelic Nxf1 proteins would alter the balance of alternative splicing either directly through interactions with splicing machinery or indirectly through an effect on transcriptional elongation rate or preference for termination site in the insertion. An alternative explanation might be for the export activity of Nxf1 to drive the nascent RNP into a territory with different relative activities for splicing and degradation, but this seems more difficult to reconcile with simultaneously increased levels of the correctly spliced message and decreased levels of the mutant splice form in five of the six suppression events.

Nxf1 protein interacts with several factors that could influence alternative splicing, including U2AF35 [45], several SR proteins [7],[8],[46],[47], and components of the TREX complex [48],[49]. Nxf1 is also recruited to the class of retroviral RNA transport elements (RTE-D), found in the IAPs we sequenced from suppressed mutations, through its interaction with RBM15 (OTT1) [42], which has also been linked to both splicing and export of Epstein-Barr virus mRNA [50]. Although these interactions are generally interpreted as recruiting export factors to mature RNPs [51], recruitment of Nxf1 to the nascent transcript through retroviral or cellular RNA transport elements could, in principle, alter the recruitment or activity of splicing factors. Both the RNA binding activity and much of the known protein interaction network around Nxf1 are conserved with respect to the Saccharomyces homolog, Mex67p [11],[48],[52]. It is interesting in this context that in splicing-specific RNA profiling of yeast mutations with defects in mRNA production the expression profile of MEX67-deficient strains cluster with transcriptional elongation factors [53]. Altered elongation rate is thought to be one mechanism that can regulate alternative splicing [54] and recruitment of Nxf1 to elongating nascent transcript could in principle alter the assembly or kinetics of other factors on the elongating pre-mRNA.

The extension of suppressor activity to a wider class of insertional mutations has several practical implications. First, these results predict that Nxf1CAST should be able to modify other mutations that involve similar IAP insertions, for which new examples continue to be reported [43],[44],[55]. Indeed, the recent description of an IAP allele of Pofut1 notes variable reduction of phenotype among F2 progeny in a cross to CAST/Ei, the strain from which the suppressing allele of Nxf1 was derived [55]. The congenic Nxf1CAST stock we have developed should be a useful tool to allow in situ titration of gene expression from either spontaneous or engineered alleles involving such insertions. Second, the range of titration in each of the six cases we have examined is ∼1.5 to 2-fold and semi-dominant. This holds over a fairly broad range of mutational effects on gene expression, ranging from ∼2% and 4% of wild-type levels (unsuppressed and suppressed, respectively) for Atcayhes to 50% and 75% for Eya1BOR. Finally, our in vivo gene titration results across six different mutations suggests that for a wide range of loci and allele strengths, even modest recovery of function may have dramatic phenotypic benefits. This is strikingly true in the case of Atcayhes, where even a 2% increment of expression has a dramatic impact on behavioral phenotype (Videos S3 and S4). This implies that for Cayman ataxia, even a small amount of recovery in biochemical or cellular function would have substantial therapeutic benefit.

We have now demonstrated suppressor activity of the Nxf1CAST allele toward six different mutations with distinct biochemical and physiological properties in the mouse. To the best of our knowledge this is now the most broadly validated suppressor or modifier gene activity in this well-studied species.

Materials and Methods


Congenic C57BL/6J (B6)–Nxf1CAST mice were derived in our laboratory [4] and maintained by backcrossing to B6. Crosses described here were initiated with a stock at N19 or later backcross generation. C3H/HeJ–AtrnmgL and B6–Mgrnmd were obtained from Dr. Teresa Gunn, Cornell University; mixed stock–Ap3d1mh2J and C3H–Atcayhes from Dr. Margit Burmeister, University of Michigan; B6–Usp14axJ from Dr. Scott Wilson, University of Alabama, Birmingham; and B6–Mitfmi-bw from Dr. Lynn Lamoreux, Texas A&M University. A/J and BALB/cJ were purchased from the Jackson Laboratory. Mice were maintained in specific pathogen-free conditions in accordance with protocols approved by the University of California at San Diego IACUC. Phenotypic comparisons were carried out using littermate pairs. Scores for behavioral phenotypes were assessed by at least 3 trained observers blinded to genotype. Videos of representative behaviors are available online as supporting information.


Genotypes for Nxf1 and each insertional mutation were determined by custom PCR assays for each locus. Conditions for PCR of full-length insertions were optimized using a commercial kit (MasterAmp Extra-Long PCR Kit, Epicentre) and primers in unique flanking sequences. DNA sequence analysis from the resulting PCR products used standard methods, as previously implemented in our laboratory [56] and assembled in Sequencher 4.8. Primers and PCR conditions are provided in the supporting information. Sequence alignments and neighbor-joining trees were performed in MUSCLE [57],[58] on the European Bioinformatics Institute web site (


Freshly dissected tissues were homogenized in Trizol reagent (Invitrogen) and processed for RNA according to the manufacturers instructions. Poly(A)+ RNA was purified by oligo(dT) cellulose chromatography. Northern blots were prepared from formaldehyde-agarose gels by capillary transfer to Hybond-N membranes and crosslinked by exposure to 2400 J UV light. Probes were prepared from cDNA fragments by random primer labeling. Hybridizations to each filter were quantified by phosphorimage analysis (Storm, Molecular Dynamics) and normalized to subsequent hybridization of Gapd to the same membrane as an internal control. Quantitative PCR assays were performed on total RNA. TaqMan assays for Atrn (Applied Biosystems, assays Mm00437738_m1 and Mm01270975_m1) and Atcay (Mm01172843_m1) were performed by the UCSD Center for AIDS Research Genomics Core Laboratory and normalized to a Gapd TaqMan assay. All other quantitative RT-PCR experiments were performed using intron-spanning primers that flank the inserted intron, detected by SYBR green fluorescence in a Bio-Rad CFX96 instrument, and quantified by the ΔΔCt method. Measurements were performed in triplicate for each sample. Samples to be compared were measured on the same plate during a single run. Custom primer sequences and conditions are provided as Tables S1, S2, S3, and S4 online.


Freshly dissected tissues were homogenized in CelLytic M Cell Lysis reagent (Sigma #C2978) plus protease inhibitors and quantified using a bichromate assay (BCA, Pierce). Samples were subjected to SDS-PAGE and Western blotting onto Hybond-ECL membranes. Antibodies and dilutions used were goat anti-Ap3d1 (Rockland, 1∶1000), rabbit anti-Caytaxin/BNIP (Gift of Dr. Low Boon Chuan [59], 1∶5000), rabbit anti-Usp14 (Bethyl Laboratories, 1∶5000). Relative levels of immunoreactivity were quantified using infrared dye-coupled secondary antibodies (Rockland, 1∶10,000) on a LI-COR imager and normalized to rabbit anti-PITPβ [40] as an internal control that correlated with BCA-measured total protein.


Summary data are plotted in figures as mean values, with error bars indicating standard deviations. For variables with expected normal distributions, including quantitative PCR experiments and behavioral observations in which several observers rated performance against a calibrated scale, hypotheses were tested using paired or unpaired t-tests depending upon whether the underlying materials were from explicitly paired samples (e.g., matched littermates) or aggregates (e.g., sibs and cousins). For variables expected to have non-normal distributions across trials (including blotting procedures, in which normalization and scaling across experiments complicate the analysis, and paired samples for which some replicate pairs represent different ages or breeding designs) hypotheses were tested using a nonparametric Wilcoxon signed-ranks test applied to replicates of paired experimental measures. Statistical calculations were carried out in Microsoft Excel or SISA online, [60] (t-tests) or using the VassarStats public web interface, (Wilcoxon tests).

Supporting Information

Video S1.

Video of behavioral phenotypes for Usp14axJ with Nxf1B6.

(0.26 MB MOV)

Video S2.

Video of behavioral phenotypes for Usp14axJ with Nxf1CAST.

(0.58 MB MOV)

Video S3.

Video of behavioral phenotypes for Atcayhes with Nxf1B6.

(1.20 MB MOV)

Video S4.

Video of behavioral phenotypes for Atcayhes with Nxf1CAST.

(0.51 MB MOV)


We gratefully acknowledge Drs. Margit Burmeister, Teresa Gunn, Scott Wilson, and Lynn Lamoreux for providing mutant mouse stocks; Dr. Low Boon Chuan for anti-BNIP-H (Caytaxin) antibody; Wendy Alcaraz, Edward Chen, Peter Gent, Chen-Jie Hong, Anchang Hu, Eunnie Kim, Tiffany Poon, and Eric Raponi for assistance in scoring behavioral phenotypes; Pinyi Du for performing TaqMan assays in the UCSD Center for AIDS Research Genomics Core Laboratory; and Dr. Xiang-Dong Fu for helpful discussions and comments on a draft manuscript.

Author Contributions

Conceived and designed the experiments: DC LFG BAH. Performed the experiments: DC LFG BAH. Analyzed the data: DC LFG BAH. Wrote the paper: DC LFG BAH.


  1. 1. Marques JT, Carthew RW (2007) A call to arms: coevolution of animal viruses and host innate immune responses. Trends Genet 23: 359–364.
  2. 2. Satterly N, Tsai PL, van Deursen J, Nussenzveig DR, Wang Y, et al. (2007) Influenza virus targets the mRNA export machinery and the nuclear pore complex. Proc Natl Acad Sci U S A 104: 1853–1858.
  3. 3. Fontoura BM, Faria PA, Nussenzveig DR (2005) Viral interactions with the nuclear transport machinery: discovering and disrupting pathways. IUBMB Life 57: 65–72.
  4. 4. Floyd JA, Gold DA, Concepcion D, Poon TH, Wang X, et al. (2003) A natural allele of Nxf1 suppresses retrovirus insertional mutations. Nat Genet 35: 221–228.
  5. 5. Yoon DW, Lee H, Seol W, DeMaria M, Rosenzweig M, et al. (1997) Tap: a novel cellular protein that interacts with tip of herpesvirus saimiri and induces lymphocyte aggregation. Immunity 6: 571–582.
  6. 6. Gruter P, Tabernero C, von Kobbe C, Schmitt C, Saavedra C, et al. (1998) TAP, the human homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. Mol Cell 1: 649–659.
  7. 7. Lai MC, Tarn WY (2004) Hypophosphorylated ASF/SF2 binds TAP and is present in messenger ribonucleoproteins. J Biol Chem 279: 31745–31749.
  8. 8. Huang Y, Yario TA, Steitz JA (2004) A molecular link between SR protein dephosphorylation and mRNA export. Proc Natl Acad Sci U S A 101: 9666–9670.
  9. 9. Segref A, Sharma K, Doye V, Hellwig A, Huber J, et al. (1997) Mex67p, a novel factor for nuclear mRNA export, binds to both poly(A)+ RNA and nuclear pores. EMBO J 16: 3256–3271.
  10. 10. Braun IC, Rohrbach E, Schmitt C, Izaurralde E (1999) TAP binds to the constitutive transport element (CTE) through a novel RNA-binding motif that is sufficient to promote CTE-dependent RNA export from the nucleus. EMBO J 18: 1953–1965.
  11. 11. Katahira J, Strässer K, Podtelejnikov A, Mann M, Jung JU, et al. (1999) The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. EMBO J 18: 2593–2609.
  12. 12. Li Y, Bor YC, Misawa Y, Xue Y, Rekosh D, et al. (2006) An intron with a constitutive transport element is retained in a Tap messenger RNA. Nature 443: 234–237.
  13. 13. Lindtner S, Felber BK, Kjems J (2002) An element in the 3′ untranslated region of human LINE-1 retrotransposon mRNA binds NXF1(TAP) and can function as a nuclear export element. RNA 8: 345–356.
  14. 14. Zolotukhin AS, Michalowski D, Smulevitch S, Felber BK (2001) Retroviral constitutive transport element evolved from cellular TAP(NXF1)-binding sequences. J Virol 75: 5567–5575.
  15. 15. Reuss FU, Frankel WN, Moriwaki K, Shiroishi T, Coffin JM (1996) Genetics of intracisternal-A-particle-related envelope-encoding proviral elements in mice. J Virol 70: 6450–6454.
  16. 16. Maksakova IA, Romanish MT, Gagnier L, Dunn CA, van de Lagemaat LN, et al. (2006) Retroviral elements and their hosts: insertional mutagenesis in the mouse germ line. PLoS Genet 2: e2.
  17. 17. Hamilton BA, Frankel WN (2001) Of mice and genome sequence. Cell 107: 13–16.
  18. 18. Dewannieux M, Dupressoir A, Harper F, Pierron G, Heidmann T (2004) Identification of autonomous IAP LTR retrotransposons mobile in mammalian cells. Nat Genet 36: 534–539.
  19. 19. Zhang Y, Maksakova IA, Gagnier L, van de Lagemaat LN, Mager DL (2008) Genome-Wide Assessments Reveal Extremely High Levels of Polymorphism of Two Active Families of Mouse Endogenous Retroviral Elements. PLoS Genet 4: e1000007.
  20. 20. Nagle DL, McGrail SH, Vitale J, Woolf EA, Dussault BJ Jr., et al. (1999) The mahogany protein is a receptor involved in suppression of obesity. Nature 398: 148–152.
  21. 21. Gunn TM, Miller KA, He L, Hyman RW, Davis RW, et al. (1999) The mouse mahogany locus encodes a transmembrane form of human attractin. Nature 398: 152–156.
  22. 22. Kim BY, Olzmann JA, Barsh GS, Chin LS, Li L (2007) Spongiform neurodegeneration-associated E3 ligase Mahogunin ubiquitylates TSG101 and regulates endosomal trafficking. Mol Biol Cell 18: 1129–1142.
  23. 23. He L, Lu XY, Jolly AF, Eldridge AG, Watson SJ, et al. (2003) Spongiform degeneration in mahoganoid mutant mice. Science 299: 710–712.
  24. 24. Phan LK, Lin F, LeDuc CA, Chung WK, Leibel RL (2002) The mouse mahoganoid coat color mutation disrupts a novel C3HC4 RING domain protein. J Clin Invest 110: 1449–1459.
  25. 25. Gunn TM, Inui T, Kitada K, Ito S, Wakamatsu K, et al. (2001) Molecular and phenotypic analysis of Attractin mutant mice. Genetics 158: 1683–1695.
  26. 26. Kantheti P, Qiao X, Diaz ME, Peden AA, Meyer GE, et al. (1998) Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles. Neuron 21: 111–122.
  27. 27. Kantheti P, Diaz ME, Peden AE, Seong EE, Dolan DF, et al. (2003) Genetic and phenotypic analysis of the mouse mutant mh2J, an Ap3d allele caused by IAP element insertion. Mamm Genome 14: 157–167.
  28. 28. Wilson SM, Bhattacharyya B, Rachel RA, Coppola V, Tessarollo L, et al. (2002) Synaptic defects in ataxia mice result from a mutation in Usp14, encoding a ubiquitin-specific protease. Nat Genet 32: 420–425.
  29. 29. Bomar JM, Benke PJ, Slattery EL, Puttagunta R, Taylor LP, et al. (2003) Mutations in a novel gene encoding a CRAL-TRIO domain cause human Cayman ataxia and ataxia/dystonia in the jittery mouse. Nat Genet 35: 264–269.
  30. 30. Nystuen A, Benke PJ, Merren J, Stone EM, Sheffield VC (1996) A cerebellar ataxia locus identified by DNA pooling to search for linkage disequilibrium in an isolated population from the Cayman Islands. Hum Mol Genet 5: 525–531.
  31. 31. Yajima I, Sato S, Kimura T, Yasumoto K, Shibahara S, et al. (1999) An L1 element intronic insertion in the black-eyed white (Mitf[mi-bw]) gene: the loss of a single Mitf isoform responsible for the pigmentary defect and inner ear deafness. Hum Mol Genet 8: 1431–1441.
  32. 32. Boissy RE, Lamoreux ML (1995) In vivo and in vitro morphological analysis of melanocytes homozygous for the misp allele at the murine microphthalmia locus. Pigment Cell Res 8: 294–301.
  33. 33. McCarthy EM, McDonald JF (2004) Long terminal repeat retrotransposons of Mus musculus. Genome Biol 5: R14.
  34. 34. Stocking C, Kozak CA (2008) Murine endogenous retroviruses. Cell Mol Life Sci 65: 3383–3398.
  35. 35. Perincheri S, Peyton DK, Glenn M, Peterson ML, Spear BT (2008) Characterization of the ETnII-alpha endogenous retroviral element in the BALB/cJ Zhx2 ( Afr1 ) allele. Mamm Genome 19: 26–31.
  36. 36. Perincheri S, Dingle RW, Peterson ML, Spear BT (2005) Hereditary persistence of alpha-fetoprotein and H19 expression in liver of BALB/cJ mice is due to a retrovirus insertion in the Zhx2 gene. Proc Natl Acad Sci U S A 102: 396–401.
  37. 37. Ho M, Post CM, Donahue LR, Lidov HG, Bronson RT, et al. (2004) Disruption of muscle membrane and phenotype divergence in two novel mouse models of dysferlin deficiency. Hum Mol Genet 13: 1999–2010.
  38. 38. Kuff EL, Lueders KK (1988) The intracisternal A-particle gene family: structure and functional aspects. Adv Cancer Res 51: 183–276.
  39. 39. Belayew A, Tilghman SM (1982) Genetic analysis of alpha-fetoprotein synthesis in mice. Mol Cell Biol 2: 1427–1435.
  40. 40. Hamilton BA, Smith DJ, Mueller KL, Kerrebrock AW, Bronson RT, et al. (1997) The vibrator mutation causes neurodegeneration via reduced expression of PITP alpha: positional complementation cloning and extragenic suppression. Neuron 18: 711–722.
  41. 41. Zolotukhin AS, Schneider R, Uranishi H, Bear J, Tretyakova I, et al. (2008) The RNA transport element RTE is essential for IAP LTR-retrotransposon mobility. Virology 377: 88–99.
  42. 42. Lindtner S, Zolotukhin AS, Uranishi H, Bear J, Kulkarni V, et al. (2006) RNA-binding motif protein 15 binds to the RNA transport element RTE and provides a direct link to the NXF1 export pathway. J Biol Chem 281: 36915–36928.
  43. 43. Sun XY, Chen ZY, Hayashi Y, Kanou Y, Takagishi Y, et al. (2008) Insertion of an intracisternal A particle retrotransposon element in plasma membrane calcium ATPase 2 gene attenuates its expression and produces an ataxic phenotype in joggle mutant mice. Gene 411: 94–102.
  44. 44. Beyer B, Deleuze C, Letts VA, Mahaffey CL, Boumil RM, et al. (2008) Absence seizures in C3H/HeJ and knockout mice caused by mutation of the AMPA receptor subunit Gria4. Hum Mol Genet 17: 1738–1749.
  45. 45. Zolotukhin AS, Tan W, Bear J, Smulevitch S, Felber BK (2002) U2AF participates in the binding of TAP (NXF1) to mRNA. J Biol Chem 277: 3935–3942.
  46. 46. Huang Y, Gattoni R, Stevenin J, Steitz JA (2003) SR splicing factors serve as adapter proteins for TAP-dependent mRNA export. Mol Cell 11: 837–843.
  47. 47. Le Hir H, Gatfield D, Izaurralde E, Moore MJ (2001) The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay. EMBO J 20: 4987–4997.
  48. 48. Strässer K, Masuda S, Mason P, Pfannstiel J, Oppizzi M, et al. (2002) TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417: 304–308.
  49. 49. Stutz F, Bachi A, Doerks T, Braun IC, Seraphin B, et al. (2000) REF, an evolutionary conserved family of hnRNP-like proteins, interacts with TAP/Mex67p and participates in mRNA nuclear export. RNA 6: 638–650.
  50. 50. Hiriart E, Gruffat H, Buisson M, Mikaelian I, Keppler S, et al. (2005) Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. J Biol Chem 280: 36935–36945.
  51. 51. Moore MJ, Proudfoot NJ (2009) Pre-mRNA processing reaches back to transcription and ahead to translation. Cell 136: 688–700.
  52. 52. Reed R, Hurt E (2002) A conserved mRNA export machinery coupled to pre-mRNA splicing. Cell 108: 523–531.
  53. 53. Burckin T, Nagel R, Mandel-Gutfreund Y, Shiue L, Clark TA, et al. (2005) Exploring functional relationships between components of the gene expression machinery. Nat Struct Mol Biol 12: 175–182.
  54. 54. Kornblihtt AR (2006) Chromatin, transcript elongation and alternative splicing. Nat Struct Mol Biol 13: 5–7.
  55. 55. Schuster-Gossler K, Harris B, Johnson KR, Serth J, Gossler A (2009) Notch signalling in the paraxial mesoderm is most sensitive to reduced Pofut1 levels during early mouse development. BMC Dev Biol 9: 6.
  56. 56. Concepcion D, Seburn KL, Wen G, Frankel WN, Hamilton BA (2004) Mutation rate and predicted phenotypic target sizes in ethylnitrosourea-treated mice. Genetics 168: 953–959.
  57. 57. Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
  58. 58. Edgar RC (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5: 113.
  59. 59. Buschdorf JP, Li Chew L, Zhang B, Cao Q, Liang FY, et al. (2006) Brain-specific BNIP-2-homology protein Caytaxin relocalises glutaminase to neurite terminals and reduces glutamate levels. J Cell Sci 119: 3337–3350.
  60. 60. Uitenbroek DG (1997) SISA-Binomial.