ࡱ > g bjbjVV 4 r< r< P + 8 @ T (! p < S U U U U U U $ " J% y i y S p B- ^ 0 (! % % % y y (! % : Text S1: Details of Participating Cohorts
Study Samples
Participants for the current analysis were drawn from 5 cohort studies, including the Atherosclerosis Risk in Communities Study (ARIC), the Cardiovascular Health Study (CHS), the Coronary Artery Risk Development in Young Adults Study (CARDIA), the Invecchiare in Chianti Study (InCHIANTI), and the Multi-Ethnic Study of Atherosclerosis (MESA). These groups comprise the CHARGE (Cohorts for Heart and Aging Research in Genome Epidemiology) Consortium. All participants provided informed consent. Local ethical committees at each institution approved the individual study protocols.
Study Samples, Phenotype, and Genotyping in the Participating Cohorts
The Atherosclerosis Risk in Communities Study
The ARIC study is a multi-center prospective investigation of atherosclerotic disease in a predominantly bi-racial population ADDIN EN.CITE 198932643264326417The Atherosclerosis Risk in Communities (ARIC) Study: design and objectives. The ARIC investigatorsAm J EpidemiolAm J Epidemiol687-70212941989/04/01African Continental Ancestry GroupArteriosclerosis/blood/epidemiology/*etiologyBlood CoagulationCohort StudiesCoronary Disease/epidemiology/mortalityEuropean Continental Ancestry GroupFemaleHospitalizationHumansMaleMiddle AgedPopulation SurveillanceProspective StudiesRisk FactorsUltrasonography/methodsUnited States1989Apr0002-9262 (Print)
0002-9262 (Linking)2646917http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=2646917eng[1]. White and African American men and women aged 45-64 years at baseline were recruited from 4 communities: Forsyth County, North Carolina; Jackson, Mississippi; suburban areas of Minneapolis, Minnesota; and Washington County, Maryland. A total of 15,792 individuals participated in the baseline examination in 1987-1989, with follow-up examinations in approximate 3-year intervals, during 1990-1992, 1993-1995, and 1996-1998.
ARIC Study samples were genotyped using the Affymetrix Genome-Wide Human SNP Array 6.0 (Santa Clara, California); for the current analysis only white participants were analyzed. Sample exclusion criteria included discordant with previous genotype data (n=83), genotypic and phenotypic sex mismatch (n=32), suspected first-degree relative of an included individual based on genotype data (n=297), genetic outlier as assessed by Identity by State (IBS) using PLINK ADDIN EN.CITE Purcell200732633263326317Purcell, S.Neale, B.Todd-Brown, K.Thomas, L.Ferreira, M. A.Bender, D.Maller, J.Sklar, P.de Bakker, P. I.Daly, M. J.Sham, P. C.Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA 02114, USA. shaun@pngu.mgh.harvard.eduPLINK: a tool set for whole-genome association and population-based linkage analysesAm J Hum GenetAm J Hum Genet559-758132007/08/19Genome, Human/*geneticsHumansLinkage (Genetics)/*geneticsPolymorphism, Single NucleotidePopulation/*genetics*Software2007Sep0002-9297 (Print)
0002-9297 (Linking)17701901http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=177019011950838S0002-9297(07)61352-4 [pii]
10.1086/519795eng[2] and >8 SD along any of the first 10 principal components in EIGENSTRAT ADDIN EN.CITE Price200632673267326717Price, A. L.Patterson, N. J.Plenge, R. M.Weinblatt, M. E.Shadick, N. A.Reich, D.Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. aprice@broad.mit.eduPrincipal components analysis corrects for stratification in genome-wide association studiesNat GenetNat Genet904-93882006/07/25AlgorithmsAllelesCase-Control StudiesDatabases, Nucleic AcidGenetic MarkersGenome, HumanGenomics/*statistics & numerical dataGenotypeHumansPhenotypePolymorphism, Single NucleotidePrincipal Component Analysis2006Aug1061-4036 (Print)
1061-4036 (Linking)16862161http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16862161ng1847 [pii]
10.1038/ng1847