Multivesicular Body Formation Requires OSBP–Related Proteins and Cholesterol

In eukaryotes, different subcellular organelles have distinct cholesterol concentrations, which is thought to be critical for biological functions. Oxysterol-binding protein-related proteins (ORPs) have been assumed to mediate nonvesicular cholesterol trafficking in cells; however, their in vivo functions and therefore the biological significance of cholesterol in each organelle are not fully understood. Here, by generating deletion mutants of ORPs in Caenorhabditis elegans, we show that ORPs are required for the formation and function of multivesicular bodies (MVBs). In an RNAi enhancer screen using obr quadruple mutants (obr-1; -2; -3; -4), we found that MVB–related genes show strong genetic interactions with the obr genes. In obr quadruple mutants, late endosomes/lysosomes are enlarged and membrane protein degradation is retarded, although endocytosed soluble proteins are normally delivered to lysosomes and degraded. We also found that the cholesterol content of late endosomes/lysosomes is reduced in the mutants. In wild-type worms, cholesterol restriction induces the formation of enlarged late endosomes/lysosomes, as observed in obr quadruple mutants, and increases embryonic lethality upon knockdown of MVB–related genes. Finally, we show that knockdown of ORP1L, a mammalian ORP family member, induces the formation of enlarged MVBs in HeLa cells. Our in vivo findings suggest that the proper cholesterol level of late endosomes/lysosomes generated by ORPs is required for normal MVB formation and MVB–mediated membrane protein degradation.


Introduction
The multivesicular body (MVB) sorting pathway provides a mechanism for the lysosomal degradation of membrane proteins and has a role in many processes, including growth factor receptor down-regulation [1], antigen presentation [2], developmental signaling [3,4], the budding of enveloped viruses [5], and cytokinesis [6,7]. MVBs form when the limiting membrane of the late endosomes invaginates and buds into the lumen of the organelle, selecting a subset of the proteins from the limiting membrane in the process [8,9]. The MVB sorting machinery is constituted by proteins that form the endosomal sorting complexes required for transport (ESCRT-I, -II, and -III) [10,11]. These ESCRT complexes are recruited sequentially to endosomal membranes where they function in sorting cargo and generating characteristic intralumenal vesicles. MVBs then fuse with lysosomes, resulting in degradation of their cargo. In addition to the ESCRT proteins, lipid molecules have been assumed to be involved in MVB formation by creating local microdomains in the endosomal membrane that induce the inward membrane curvature. For example, lysobisphosphatidic acid (LBPA) and ceramide were shown to induce the formation of internal vesicles in liposomes [12,13]. Furthermore, treatment with anti-LBPA antibodies disrupts normal MVB formation in mammalian cells, suggesting that LBPA has a role in driving lumenal-vesicle formation at the cellular level [14].
In eukaryotes, different organelles within a cell generally have distinct cholesterol concentrations. Such differences are thought to be necessary for various biological functions ranging from membrane trafficking to signal transduction [15]. Obtaining the normal subcellular cholesterol distribution is thought to require a variety of intracellular cholesterol movements through vesicular and nonvesicular mechanisms [16,17]. Recently, oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) have been shown to mediate a number of cellular processes including signal transduction, lipid metabolism, vesicular trafficking and nonvesicular sterol transport [18][19][20]. OSBP was first identified as a high-affinity cytosolic receptor for oxysterols, such as 25-hydroxycholesterol [21]. Subsequently, most eukaryotes have been shown to have proteins homologous to OSBP, including 12 ORP-homologs in humans (OSBP and ORP1 to ORP11), four in C. elegans (this study; OBR-1 to OBR-4), four in D. melanogaster, and seven in the budding yeast S. cerevisiae (Osh1p to Osh7p) [19,22]. Most ORPs share two highly homologous structural features: a PH domain at the amino-terminus and a ,400-amino acid sterol-binding domain at the carboxyterminus ( Figure S1) [19]. The mammalian ORP family can be subdivided into six subfamilies (I-VI) based on gene organization and amino acid homology. Yeast ORPs share comparatively low sequence homologies with mammalian ORP proteins and are not classified into the ORP subfamilies, whereas C. elegans and D. melanogaster ORPs clearly fall into subfamilies I, II, IV and V based on the homology of the sterol-binding domains (Figures S1, S3, S4, S5, S6).
Many lines of evidence suggest that ORPs have a role in sterol distribution among intracellular organelles. Raychaudhuri showed that yeast ORPs (Osh4p, Osh5p, and Osh3p) have a role in transporting sterol from the yeast plasma membrane to the esterification compartment, ER [18]. In addition, the cholesterol distribution in yeast ORPs mutants was abnormal. A crystal structure analysis indicated that Osh4p is able to accommodate a variety of sterols including cholesterol [23]. In in vitro analyses, Osh4p and mammalian ORPs transferred sterols from donor to acceptor liposomes [18,24]. In mammalian cells, the transport of newly synthesized cholesterol from the ER to the cell surface is enhanced by expression of ORP2 [25]. Although increasing evidence supports the involvement of ORP proteins in subcellular cholesterol distribution, knockout studies of ORPs in animals have not been reported, and consequently, the biological significance of distinct cholesterol concentrations in subcellular compartments remains to be elucidated.
In the present study, we generated deletion mutants of all ORP family members in C. elegans (obr-1, -2, -3, and obr-4). We also performed an RNAi modifier screen using obr quadruple mutants and found that a group of MVB-related genes including ESCRT complex genes show strong genetic interactions with obr genes.

Generation of deletion mutants of C. elegans ORP family members
A database search revealed the presence of four ORP family members in C. elegans, which are classified into ORP subfamilies I, II, IV and V based on the homology of the sterol-binding domains. We named these ORP genes obr-1, obr-2, obr-3, and obr-4, respectively [obr: Oxysterol Binding protein (OSBP) Related ( Figure S1, S3, S4, S5, S6) [19]]. To address the functions of ORP members, we generated deletion mutants of all four ORP genes in C. elegans by PCR-based screening of TMP/UVmutagenized libraries ( Figure S2) [26]. All of these mutations appear to be null or strong loss-of-function alleles because inhibition of each obr gene by RNAi failed to enhance the obr quadruple mutant phenotypes, such as embryonic lethality and slow growth as described below.
Single mutant worms with deletions in obr-1, obr-2, obr-3, or obr-4 were viable and fertile, and displayed an essentially normal phenotype under a dissection microscope ( Table 1). The obr-1;obr-2;obr-3;obr-4 quadruple mutants that lacked all obr genes exhibited embryonic lethality (,11%) and slow growth during larval development (,18%) ( Table 1). Hatched obr quadruple mutants were able to develop to adults and produce subsequent progeny, although they had a reduced brood size (60% of that of wild-type worms) and showed abnormal cuticle structure ( Figure S7B and S7D). These data indicate that four C. elegans ORP proteins act redundantly during embryonic and larval development. This is similar to the case in yeast where any one of the 7 ORPs is sufficient for viability [27].

Knockdown of multivesicular body (MVB)-related genes enhances embryonic lethality of obr quadruple mutants
To gain insights into the molecular mechanisms of embryonic lethality in obr quadruple mutants, we conducted a synthetic lethal screen. We used feeding RNAi clones on chromosomes I and III in the Ahringer library to identify RNAi clones that cause embryonic lethality in the obr quadruple mutant background, but not in the wild-type background (see Materials and Methods, and Table S1). As a result, we identified 28 genes that showed synthetic lethality in obr quadruple mutants (Table S2, hereafter, we refer to obr-1;obr-2;obr-3;obr-4 quadruple mutants as the ''obrs mutants''). These enhancer genes included genes encoding vesicular transportrelated proteins, signaling proteins, and nuclear proteins. Interestingly, among the 28 enhancer genes, 6 genes (hgrs-1, vps-28, vps-2, vps-20, vps-4, and vps-34) have been reported to function in the formation of multivesicular bodies (MVBs), the machinery for degrading membrane proteins ( Figure 1). Knockdown of vps-4 caused complete embryonic lethality in the obrs mutants as compared to 13% embryonic lethality in wild-type animals ( Figure 1). RNAi against other enhancer genes (hgrs-1, vps-28, vps-2, vps-20, and vps-34) also showed remarkably increased embryonic lethality (50-80%) in the obrs mutants as compared to wild-type worms (0-10%) under the present feeding RNAi conditions ( Figure 1). In the eri-1(mg366); lin-15B(n744) background, which is hypersensitive to RNAi, knockdown of vps-4, hgrs-1, vps-28 or vps-32.2 (a component of ESCRT-III) resulted in embryonic lethality with high penetrance in wild-type worms, indicating that ESCRT components are essential for embryonic development in C. elegans [28] (data not shown).
Formation of MVBs requires the components of four complexes that include Vps27 (sometimes referred to as ESCRT-0), ESCRT-I, ESCRT-II, and ESCRT-III [10]. These complexes are recruited sequentially to endosomal membranes where they function in sorting cargo and generating intralumenal vesicles. The 6 obr enhancer genes encode C. elegans homologues of the ESCRT components or their regulatory molecules. These include hgrs-1, a homologue of yeast Vps27, vps-28, a component of ESCRT-I, vps-2 and vps-20, components of ESCRT-III, vps-4, a homologue of yeast Vps4/AAA ATPase that is recruited by ESCRT-III to disassemble

Author Summary
The multivesicular body (MVB) sorting pathway provides a mechanism for the lysosomal degradation of membrane proteins, such as growth factor receptors. The formation of MVBs is unique in that the curvature is directed toward the lumen of the compartment rather than the cytosol. During MVB formation, the curvature-inducing proteins, such as clathrins, could not be involved in the inward invagination of the endosomal membrane. Under these circumstances, lipids have been assumed to play a role in the membrane invagination step by creating local membrane environments; however, the lipids involved in this step have not been fully elucidated. Here we demonstrate that cholesterol, an essential membrane component in animals, is critical for MVB formation and function. We found that disruption of OSBP-related proteins (ORPs), which have been proposed to function in cellular cholesterol distribution and metabolism, reduces the cholesterol content in late endosomes/lysosomes, leading to impaired MVB function. MVB sorting pathway is known to be involved in many processes, including growth factor receptor down-regulation, exosome secretion, antigen presentation, the budding of enveloped viruses, and cytokinesis. Our findings provide a novel link between cholesterol and these biologically important functions. Figure 1. Synergism between obr genes and genes related to MVB formation in embryogenesis. The graph depicts the effects of suppression of MVB-related genes on the viability of wild-type and obr quadruple mutants (obr-1;2;3;4). Non-viable animals died at the embryo stage and were scored as described in Materials and Methods after feeding RNAi against the indicated genes. Error bars represent the standard error of the proportion. doi:10.1371/journal.pgen.1001055.g001 and recycle the ESCRT machinery, and vps-34, a class III phosphoinositide 3 (PI3) kinase required for recruitment of ESCRT-0 to early endosomal membranes. ZK930.1, a homologue of mammalian p150 that encodes the PI3 kinase regulatory subunit, was also identified as an obr enhancer gene ( Figure 1; Table S2).
obr quadruple mutants exhibit enlarged late endocytic compartments A strong genetic interaction between obr genes and MVBrelated genes led us to hypothesize that late endocytic compartments (late endosomes/lysosomes) are affected in obrs mutants. In S. cerevisiae, disruption of an MVB-related gene, such as vps-4 or vps-28, causes enlargement of aberrant late endocytic compartments and disturbance of membrane protein degradation [29]. To assess the morphology of late endocytic compartments in obrs mutant embryos, we first used the fluorescent probe LysoSensor Green, which accumulates in acidic compartments because of protonation [30]. In wild-type embryos, the probe localized to small punctate vesicles throughout embryogenesis (Figure 2A). In contrast, in obrs mutant embryos, the number of large fluorescent vesicles increased, indicating that late endocytic compartments were enlarged in obrs mutant embryos ( Figure 2B). Knockdown of vps-4 in wild-type embryos also caused the appearance of similar large fluorescent vesicles as observed in obrs mutant embryos ( Figure 2C), and these enlarged vesicles were synergistically increased in the obrs mutant background ( Figure 2D, Figure  S12A). These data indicate that in obrs mutant embryos, late endocytic compartments were enlarged and these morphological defects were enhanced by knockdown of the MVB-related genes.
We next examined the expression of LET-23, a C. elegans homologue of the epidermal growth factor (EGF) receptor which is known to be degraded via the MVB pathway [1]. In wild-type embryos, LET-23::GFP was observed mostly in small punctate vesicles (,0.7 mm) during embryonic morphogenesis ( Figure 2I and 2Q). In obrs mutants, LET-23::GFP vesicles were enlarged and GFP intensity was stronger than that in wild-type embryos ( Figure 2J). A portion of LET-23::GFP-positive vesicles colocalized with the LysoTracker-labeled endosomes/lysosomes ( Figure  S12G, S12H, S12I). Knockdown of vps-4 in wild-type embryos also caused the appearance of large vesicles similar to those observed in obrs mutant embryos ( Figure 2K and 2Q). These observations indicate that LET-23::GFP was partly localized to late endocytic compartments in obrs mutants, although it is possible that some of the LET-23::GFP was localized to compartments other than endosomes/lysosomes. In the obrs mutant background, the GFP level was synergistically increased by knockdown of an MVB-related gene such as vps-4, hgrs-1 or vps-28 ( Figure 2L and 2Q). These results suggest that degradation of the EGF receptor LET-23 was retarded in obrs mutants and this defect was synergistically enhanced by knockdown of MVB-related genes.
Next, we investigated the fluid-phase endocytosis in obr mutants using a transgenic strain that secretes GFP from the muscle into the body cavity (myo-3p::ssGFP) [31]. In wild-type animals, the secreted soluble GFP (ssGFP) was rapidly endocytosed by the coelomocytes and degraded ( Figure 3I and 3J) [31]. However, mutants defective in endocytosis or intracellular transport of endocytosed soluble proteins in coelomocytes showed increased levels of ssGFP in the body cavity [31]. In the obrs mutants, ssGFP appeared to be efficiently endocytosed by coelomocytes, producing animals with bright green coelomocytes as observed in wild-type worms ( Figure 3K and 3L). To obtain higher temporal resolution, we microinjected Texas-red BSA into the body cavity of the worms [31,34]. In wild-type worms, 20 min after injection, the marker started accumulating in the late endosomes of coelomocytes as indicated by the RME-8::GFP-positive compartments ( Figure 3M). By 60 min, it was observed increasingly in lysosomes but was absent from RME-8::GFP-positive late endosomes ( Figure 3N and 3O). In obrs mutants, the fluid-phase endocytosis and postendocytic trafficking proceeded with the same kinetics as observed in wild-type worms ( Figure 3P-3R). We also checked receptor-mediated endocytosis of a yolk protein VIT-2 in oocytes (Rme) [35], and found that VIT-2 was efficiently incorporated into oocytes in the obrs mutants in a similar manner to that in wild-type worms ( Figure S8A and S8C). Taken together, these data indicate that endocytic trafficking of soluble proteins to lysosomes is not affected in obrs mutant coelomocytes.
obr quadruple mutants are delayed in the degradation of membrane protein We next examined internalization and subsequent degradation of cell surface membrane proteins in the obrs mutants. To this end, we used a transgenic worm expressing a member of the caveolin protein family, CAV-1, that has been reported to be degraded via the MVB pathway during the oocyte-to-embryo transition [36]. In control oocytes prior to fertilization, CAV-1::GFP was concentrated in intracellular vesicles ( Figure 4A, an oocyte indicated by ''21'') [37]. Immediately after oocytes passed through the spermatheca and were fertilized, the CAV-1::GFP signal of intracellular vesicles was lost and the CAV-1::GFP signal on plasma membrane rapidly increased ( Figure 4A, an embryo indicated by ''+1''). Most of CAV-1::GFP was internalized and degraded in the one-cell stage embryo and was not observed beyond the two-cell stage ( Figure 4A, Figure S10A and S10B, embryos indicated by ''+2'' to ''+4''). The post-fertilization increase in the amount of CAV-1::GFP on the cell surface and its subsequent re-internalization were not affected either in the obrs mutants or vps-4 RNAi worms (''+1'' and ''+2'' embryos in Figure 4A-4C). Consistent with previous results [36], knockdown of an MVB-related gene, such as vps-4, hgrs-1, vps-28, or vps-20, resulted in a substantial delay in the degradation of internalized CAV-1::GFP, which remained on internal membranes even in the ''+5'' embryo (an embryo at about the 26-cell stage) ( Figure 4B and data not shown). The obrs mutants exhibited slightly but significantly retarded degradation of internalized CAV-1::GFP, where significant CAV-1::GFP signal was observed in intracellular membranes of +2 and +3 embryos ( Figure 4C). A western blot analysis also revealed that the amount of CAV-1::GFP increased in the obrs mutants ( Figure 4D). The milder defects in CAV-1::GFP degradation in the obrs mutants than in vps-4 RNAi worms indicate that obr genes are not essential for the degradation of membrane proteins, but are required for efficient degradation of those proteins in C. elegans embryos.

Knockdown of MVB-related genes causes high embryonic lethality under cholesterol-depleted conditions
Because ORPs have been implicated in intracellular cholesterol transport, we tested the possible involvement of cholesterol in MVB formation. C. elegans requires cholesterol for normal development, but does not possess the enzymes necessary for de novo sterol biosynthesis. Therefore C. elegans membrane cholesterol must be supplied by the diet [38]. The first generation of wild-type worms placed on cholesterol-depleted plates develop from eggs to adults without external cholesterol because cholesterol is supplied from mother worms grown on normal plates (Brenner condition; 5 mg/ml of cholesterol). However, 5% of second-generation embryos died ( Figure 5A) and the development of all hatched larvae was arrested at the early larval stage (data not shown) [39]. Under these cholesterol-restricted conditions, second-generation obrs mutants exhibited 96% embryonic lethality whereas the mutants showed only 11% embryonic lethality under cholesterolsupplemented conditions ( Figure 5A). The hypersensitivity of obrs mutants to cholesterol deprivation suggests that the OBR proteins are involved in the utilization of cholesterol in C. elegans.
We next performed knockdown of MVB-related genes under cholesterol-restricted conditions (see Materials and Methods). Under cholesterol-restricted conditions, knockdown of MVB-related genes, such as hgrs-1 and vps-4, resulted in remarkably reduced viability and high penetrance embryonic lethality ( Figure 5A). The reduced viability of hgrs-1(RNAi) and vps-4(RNAi) worms under cholesterolrestricted conditions is similar to that observed in the obrs mutant background (Figure 1). These results suggest that cholesterol content is critical for MVB formation during embryogenesis and that obr molecules regulate cholesterol content in C. elegans.
To examine whether the late endosomal/lysosomal defects observed in obrs mutants occur in wild-type worms under cholesterol-restricted conditions, we again used LysoSensor Green to visualize late endocytic compartments. As observed in obrs mutants ( Figure 2B), late endocytic compartments were enlarged under the cholesterol-restricted conditions ( Figure 5C). We also found that LET-23::GFP vesicles were enlarged and their GFP intensity was stronger under cholesterol-restricted conditions than under cholesterol-supplemented conditions ( Figure 5F and 5G). These data indicate that cholesterol is essential for the normal morphology of late endocytic compartments and for the degradation of membrane proteins via MVB formation.
To examine the cholesterol content of the late endocytic compartments, wild-type and obrs mutants were fed with radioactive cholesterol and homogenized with a Dounce homogenizer device [40]. The crude membrane fraction (20,0006g ppt in Figure S11A) was subjected to density gradient centrifugation by using a Lysosome Isolation Kit (see Materials and Methods). ER and Golgi membranes were found in the high-density fractions ( Figure S11A; fractions #1-4, PAF-2 and COGC-3, respectively) and late endosomes/lysosomes were recovered in the low-density fractions ( Figure S11A; fractions #7, 8, RAB-7::GFP). In wild-type animals, appreciable amount of radioactive cholesterol was recovered in the late endosomal/lysosomal fractions (fractions #7 and #8), whereas the cholesterol content in the late endosomal/lysosomal fractions of the obrs mutants was approximately 75% of that of wild-type worms ( Figure S11A and S11B). The total cholesterol content in obrs mutants was also reduced significantly (to ,60% of that of wild-type, Figure S11C), indicating that ORPs are also important for determining the cholesterol content of C. elegans.

ORP1L, a mammalian homologue of obr-2, is required for the formation of MVBs in HeLa cells
Finally, we examined whether the functions of C. elegans obr members are conserved across species. We expressed all human ORP family members in HeLa cells and found that only ORP1L localized at lysosomes (data not shown) as reported previously [41]. ORP1L is structurally classified to ORP subfamily II which includes C. elegans obr-2 ( Figure S1 and Figure S4). To determine the effects of ORP1L depletion on late endosomal/lysosomal morphology, we analyzed the morphology at the ultrastructural level by electron microscopy. In control cells, late endosomal/ lysosomal compartments appeared as relatively dense round structures of 0.2-to 1-mm diameter, in which numerous small vesicles (MVBs) could be seen ( Figure 6B and 6C). In contrast, large swollen vacuoles of 0.6-to 1.8mm diameter appeared in ORP1L siRNA-treated cells ( Figure 6A and 6D-6F). These enlarged structures appeared to be MVBs because they still contained some intralumenal vesicles, although significantly less in number compared with the intralumenal vesicles in MVBs of control cells. Furthermore, ORP1L siRNA-treated cells had ,30% less MVBs than control cells ( Figure 6G). We next investigated whether depletion of ORP1L affects EGF receptor degradation (see Text S1). In HeLa cells treated with the control siRNA, the EGF receptor was gradually degraded after 1, 2, and 3 hr of EGF stimulation. siRNA against ORP1L delayed EGFinduced receptor degradation more than the control siRNA ( Figure S13A and S13B). In conclusion, these results indicate that ORP1L is required for MVB formation, normal morphology of late endosomes/lysosomes and membrane protein degradation, and these functions are evolutionarily conserved in mammals.

ORP proteins facilitate the formation of MVBs and subsequent membrane protein degradation
Cholesterol is a structural component of animal membranes that influences fluidity, permeability and formation of lipid microdomains. ORP family members have been implicated in  the cholesterol distribution among intracellular organelles [18][19][20][21][22][23][24][25]; although their in vivo functions are not fully understood. In the present study, we generated deletion mutants of all ORP family members in C. elegans (obr-1, -2, -3, and obr-4) ( Figure S2; Table 1). We also performed an RNAi modifier screen using obr quadruple mutants (obrs mutants) and found that a group of MVB-related genes including ESCRT complex genes show strong genetic interactions with obr genes (Figure 1; Table S2).
In obrs mutants, degradation of membrane proteins, such as an EGF receptor (LET-23::GFP) ( Figure 2I-2L) and caveolin (CAV-1::GFP) (Figure 4), is delayed and late-endosomes/lysosomes are enlarged (embryos; Figure 2B, coelomocytes; Figure 3B and 3E). At the ultrastructural level, obrs mutants have enlarged vacuoles which are not observed in wild-type worms ( Figure S7A and S7B). Similar defects of endocytic compartment have been reported in ESCRT-depleted S. cerevisiae [29] and mammalian cells [42,43], in which MVB formation is impaired. These observations indicate that ORP molecules are required for efficient membrane protein degradation via the MVB sorting pathway. On the other hand, endocytosed soluble proteins, such as GFP and Texas-red BSA, are normally delivered to lysosomes and are efficiently degraded in obrs coelomocytes ( Figure 3K, 3L, and 3P-3R). This data indicate that, at least in obrs coelomocytes, endocytic trafficking from the plasma membrane to lysosomes is not affected and that fusion of late endosomes and lysosomes occurs normally to generate mature lysosomes. Together, these observations suggest that ORP molecules are selectively involved in the degradation of membrane proteins via the MVB sorting pathway. In this study, we analyzed embryonic epithelial cells ( Figure 2I-2Q, Figure 5F and 5G) and fertilized eggs (Figure 4) to examine the degradation of membrane cargos (LET-23::GFP and CAV-1::GFP, respectively), and analyzed coelomocytes ( Figure 3I-3R) to examine the degradation of lumenal cargos (GFP and Texas-red BSA). The finding that lumenal cargos are normally degraded while membrane cargos are not may be because of tissue differences rather than differences in the cargospecific functions of ORPs. Therefore, further analyses will be needed to determine if ORPs are involved in the degradation of lumenal cargos in general.

Sufficient cholesterol is required for MVB formation
How are ORP molecules involved in MVB formation? In the present study, we showed that the total cholesterol content in obrs mutants was significantly reduced compared to wild-type worms, indicating that ORPs are important for utilization of cholesterol in C. elegans ( Figure S11C). We also demonstrated that the cholesterol content of late endosomes/lysosomes was reduced in obrs mutants ( Figure S11A and S11B). How C. elegans ORPs control the intracellular cholesterol level is unclear at this time. As mentioned above, ORPs are implicated in many cellular processes including signal transduction, cholesterol metabolisms, vesicular transport and nonvesicular sterol transport [20]. One possibility is that ORPs is involved in cholesterol transport to late endosomes/lysosomes directly by binding cholesterol or indirectly by regulating other cholesterol-binding proteins. ORPs may also control intracellular signaling and/or vesicular transport that determine the cholesterol content among intracellular organelles.
In obrs mutants, knockdown of MVB-related genes remarkably increased embryonic lethality (Figure 1). Knockdown of MVBrelated genes also induces high penetrance embryonic lethality under cholesterol-restricted conditions ( Figure 5A). Furthermore, late-endosomes/lysosomes are enlarged in both obrs mutants and cholesterol-restricted worms ( Figure 2B and Figure 5C). These observations suggest that in obrs mutants, reduction of late endosomal/lysosomal cholesterol content disturbs MVB formation to some extent, and leads to hypersensitive lethality when the expression of MVB-related genes is knocked down. Another possibility is that the reduced cholesterol content in late endosomes/lysosomes indirectly affects MVB function. For example, the reduced cholesterol content might inhibit Golgi-tolysosome transport of proteins that are required for MVB formation.
In addition to acting as cholesterol transfer proteins, ORPs have also been proposed to act as a sterol sensor that controls cell signaling [44]. Furthermore, two yeast ORPs (Osh6p and Osh7p) have been shown to interact with Vps4p, which has a role in dissociating the ESCRT-III complex from the endosomal membrane [45], suggesting that ORPs directly regulate ESCRT function in response to the cellular cholesterol content. We found that the localization of an ESCRT-III component (VPS-20) is not affected in obrs mutants ( Figure S12J, S12K, S12L) and that the localization of mCherry::OBR-2, which fully restores the lysosomal morphology of obrs mutants ( Figure S12B, S12C, S12D, S12E, S12F), is not altered by knockdown of the MVB-related genes (data not shown). Further studies are needed to determine whether ORPs are directly involved in ESCRT function.
The formation of MVBs is unique in that it is directed toward the lumen of the compartment, rather than the cytosol [46]. During MVB formation, curvature-inducing proteins, such as clathrins and coat protein complexes, could not be involved in the inward invagination of the endosomal membrane. It is also unlikely that the ESCRT proteins directly induce the invagination of the endosomal membrane without getting trapped in the lumen of the forming vesicles. Under these circumstances, lipids have been assumed to play an important role in the membrane invagination step by creating local membrane environments [47]. In mammalian cells, cholesterol is concentrated in endosomal/ lysosomal compartments, especially in the luminal vesicles of MVBs [48]. C. elegans also has a considerable amount of cholesterol in the endosomal/lysosomal fraction ( Figure S11A and S11B). However, the mechanism for accumulation of cholesterol in endosomes/lysosomes is largely unknown, and consequently, the biological significance of cholesterol in endosomal/lysosomal compartments has not been fully elucidated. In this study, we showed that disruption of ORPs reduces the cholesterol content in the endosomal/lysosomal compartments and impairs the MVB formation and function. Although it is not clear at present that the decrease in the cholesterol content is a direct cause of MVB abnormalities, the present study lay a firm basis for further work to more fully elucidate how cholesterol is involved in MVB formation.
through fluid-phase endocytosis in RME-8::GFP-expressing coelomocytes. The outline of the coelomocyte is indicated by a white line (M-R). (M and P) 20 minutes after injection of BSA-TR, BSA-TR appears within RME-8::GFP-positive late endosomes in wild-type and obr quadruple mutants. (N and Q) After 40 min, some TR-BSA fills RME-8::GFP-labeled vesicles at a low level, whereas some TR-BSA is concentrated into RME-8::GFP-negative vesicles in wild-type and obr quadruple mutants. (O and R) After 60 min, most of the BSA-TR appears in RME-8::GFP-negative lysosomes in wild-type and obr quadruple mutants. Note that RME-8::GFP-negative lysosomes are enlarged in obr quadruple mutant coelomocytes (Q and R, arrows). Scale bars are 5 mm (M-R). doi:10.1371/journal.pgen.1001055.g003 In C. elegans, cholesterol depletion induces multiple responses such as embryonic lethality, dauer larva formation, and molting defects [38,39]. Dauer larva formation is regulated by steroid hormone signaling, in which cholesterol-metabolizing enzymes DAF-36 (Rieske-like oxygenase) and DAF-9 (Cytochrome P450) are thought to convert cholesterol into steroid hormones, such as 4-dafachonic acid, that act on a steroid hormone receptor, DAF-12 [49,50]. C. elegans molting is also thought to be regulated by cholesterol-derived steroid hormones via a steroid hormone receptor, NHR-25 [51]. We have never observed dauer larva formation or molting defects in obrs mutants, suggesting that obr mutations do not affect signaling by these steroid hormones.

An evolutionarily conserved role of ORPs
In this study, we demonstrated that human ORP1L is required for MVB formation in mammalian cells. A previous study demonstrated that the GTPase Rab7, when bound to GTP, simultaneously binds to ORP1L and RILP to form a RILP-Rab7-ORP1L complex, which is required for the perinuclear localization of late endosomes/lysosomes [52,53]. Mammalian ORP1L contains three ankyrin repeats at the amino-terminal end, and the interaction with Rab7 through the ankyrin repeats of ORP1L is essential to specify the perinuclear localization of late endosomes/ lysosomes ( Figure S1) [41]. In C. elegans and D. melanogaster, the obr gene products lack the amino-terminal ankyrin repeats and the late endosomes/lysosomes are not organized into the characteristic perinuclear cluster observed in mammalian cells ( Figure S1). These observations suggest that the fundamental role of ORP1L is to maintain enough cholesterol in late endosomes/lysosomes for normal MVB formation. They also suggest that the perinuclear localization of late endosomes/lysosomes in mammals is the result of the appearance of the amino-terminal ankyrin repeats of ORP1L.
As mentioned above, MVB formation requires the inward invagination of the endosomal membrane. Similar membrane invagination also occurs in exosome formation, cytokinesis and viral budding. There is accumulating evidence that the ESCRT proteins have a role in this type of membrane fission. HIV budding from the plasma membrane also requires ESCRT proteins such as Hrs, a homologue of hgrs-1. Interestingly, it has been reported that HIV envelopes contain a high level of cholesterol and cholesterol depletion impairs HIV-1 budding at the plasma membrane. Further studies are needed to assess the involvement of ORP proteins in this process.
Other enhancers whose knockdown causes synthetic lethality with obr mutations In addition to 6 MVB-related genes (hgrs-1, vps-28, vps-2, vps-20, vps-4, and vps-34), we identified 22 other genes that showed synthetic lethality in obr quadruple mutants (Table S2). At the present time, the reason for the strong interaction between these 22 genes and obr genes is unclear. However, like MVB-related genes, several enhancer genes may require a cholesterol-rich membrane environment for their normal functions. Cholesterolrich microdomains play important roles in several biological functions, such as raft-dependent cellular signaling and caveolaemediated endocytosis at the plasma membrane [15]. The present study suggested a novel role of cholesterol-rich microdomains, i.e. providing an adequate membrane environment for MVB formation. Further studies of the enhancer genes should uncover other aspects of intracellular cholesterol functions.

Phenotypic analysis
Adult wild-type and mutant worms were allowed to lay eggs for 2-3 hr, and the progeny were scored for embryonic lethality and larval arrest. Unhatched eggs were examined 24 hr after being laid, and hatched but arrested larvae were examined 72 hr after being laid. To perform fluid-phase endocytosis assay, Texas red BSA was injected at 1 mg/ml in water into the body cavity of wildtype or obr quadruple mutants expressing RME-8::GFP. At defined time points, animals were mounted on slides, put on ice to stop endocytosis, and fluid-phase internalization of the dye into the coelomocytes was viewed with a confocal microscope. For the quantification of endosomes and lysosomes sizes, discrete intracellular structures in at least 30 coelomocytes were analyzed for each marker (RME-8::GFP for late endosomes, LMP-1::GFP for lysosomes). Individual sections through coelomocyte were scanned, and the diameter of the largest endosomes or lysosomes was scored. Coelomocyte, endosomes and lysosomes areas were calculated from their diameter. To quantify the size of LET-23::GFP-positive endocytic compartments in embryos, LET-23::GFP-positive endocytic compartments were sorted into three size categories according to their diameter: 0.7mm.(normal), 0.7-1.5 mm (weak enlarged), and 1.5 mm,(strong enlarged).

RNAi feeding screen
Feeding RNAi was performed as described previously [55]. To score embryonic lethality, young adult worms were placed on each RNAi plate and allowed to feed for 24 hr. Three worms from the original plate were transferred to a fresh RNAi plate and were allowed to lay eggs for 4-5 hr to score embryonic lethality. In an RNAi screen, we first used feeding RNAi clones on chromosome I and III in the Ahringer library to identify RNAi clones that cause high penetrance embryonic lethality in the obr quadruple mutant background, but not in the wild-type background. As a result, we found 22 RNAi clones that caused synthetic lethality with obr quadruple mutations (Table S2, Group A). These enhancer genes included the genes encoding vesicular transport-related proteins, such as apm-1 (m subunit of AP-1), arf-1.2 (a homologue of ARF), vps-34 (Class III phosphatidylinositol 3 kinase) and vps-2 (ESCRT III). Therefore we next focused on genes whose homologues are known to regulate intracellular vesicular transport in other species (Table S2) [56] (MVB formation-related genes, small G proteins, components of COG complex, SNARE genes, SEC-1 family genes, coatmer proteins, and components of retromer complex). We tested 113 genes listed in Table S1 and identified another six genes that could enhance embryonic lethality of obr quadruple mutants (Table S2, Group B).

Cholesterol depleted condition in C. elegans
To obtain cholesterol-free conditions, agar was replaced by agarose S (Wako, Japan) and peptone was omitted from plates. An overnight culture of the OP50 strain of E. coli was grown on a LB medium. Bacteria were rinsed with M9 medium before use. Bacterial suspension were spread on cholesterol-free agarose plates. To perform RNAi under cholesterol depleted condition, bacteria were grown at 37uC to an O.D. of 0.5-0.8, induced with 0.4mM IPTG for 4hr, then concentrated and spread onto agarose plates containing 0.4mM IPTG. For feeding P0 animals, L4 hermaphrodites were plated directly on these plates at 20uC and their progeny were analyzed.

Microscopy
Fluorescence images were obtained using an Axio Imager M1 (Carl Zeiss MicroImaging Inc., Japan) microscope equipped with a digital CCD camera. Confocal images were obtained using a Zeiss LSM510 META confocal microscope system (Carl Zeiss Micro-Imaging).

Preparation of polyclonal antibody
A glutathione S-transferase-ORP1L fusion protein corresponding to amino acids 428-553 in the ORP1L protein was expressed in E. coli BL21 (DE3), purified by affinity chromatography on glutathione-Sepharose 4B (Pharmacia AB, Uppsala, Sweden), and used for immunization of New Zealand White rabbits according to a standard protocol. The ORP1L antiserum were purified by using an Affi-Gel (BIO-RAD, Japan) to which the antigen fragment had been coupled. The antibody were used for immunoblotting in 1 : 10 dilution.

Electron microscopy
HeLa cells cultured on plastic cover glass (Celldesk LF1, Sumitomo Bakelite inc, Tokyo, Japan) in 24-well culture plates were fixed with 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) for 2 hr. Cells were post-fixed in 1% OsO 4 in the same buffer for 1 hr, and dehydrated with a series of ethanol and embedded in epon. After the resin hardened, Celldesk was removed from the epon block. Ultra-thin sections were cut horizontally to the bottom of Celldesk, stained with uranyl acetate for 60 minutes, stained with lead citrate solution for 1 min, and observed under a Hitachi H-7600 electron microscope. For quantitative analyses, electron micrographs were taken at a magnification of 12,000. The cytoplasmic area and the number and diameter of MVBs were determined. Ten cell profiles were taken from each Celldesk, and three samples were analyzed (a total of 30 cells). C. elegans were pre-fixed with 4% paraformaldehyde and 1% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4). Samples were then cut into small pieces, fixed again with 2% paraformaldehyde and 2% glutaraldehyde in the same buffer, and post-fixed with 2% osmium tetroxide in phosphate buffer for 4 hrs. Afterwards, fixed specimens were dehydrated in a graded series of ethanol and embedded in Quetol 651 epoxy resin. Ultrathin (80 to 90 nm-thick) sections obtained by ultramicrotomy were stained with uranyl acetate for 15 minutes and with modified Sato's lead solution for 5 mins. TEM observation was performed using a JEOL JEM-1200EX electron microscope.

Measurement of cholesterol level in late endosomes/ lysosomes in C. elegans
Synchronized first-stage larvae (40,000 worms) were cultured with 6 mCi of [ 14 C]-cholesterol (54 mCi/mmol; American Radiolabeled Chemicals, Inc. St. Louis, U.S.A.) for 54 hr on cholesterol free agar plates (see above) and were harvested from the plates with M9 medium. Late endosomal/lysosomal fraction was then prepared using the lysosome isolation kit (Sigma). Briefly, worms were homogenized using a Dounce homogenizer device and the lysates were subjected to centrifugation at 1,0006g to remove the nuclei. The post nuclear supernatant was subjected to centrifugation at 20,0006g to pellet the membranes, yielding the crude membrane fraction. The crude membrane fraction was resuspended in extraction buffer and subjected to density gradient ultracentrifugation at 150,0006g on an 8-27% Optiprep gradient for 4 hr (Lysosomal Isolation Kit, Sigma-Aldrich). 250 ml fractions were collected from the bottom of the tube with a peristaltic pump. The resulting fraction was treated with 250 mM calcium chloride to remove residual mitochondria and rough ER. Aliquots were assayed for lipid analysis, and the remaining material was processed for immunoblotting. Lipids were extracted by hexane, and were separated by one-dimensional TLC on silica gel 60 plates (Merck Biosciences) in chloroform-methanol (24:1). Cholesterol was identified by comigration with known standard. Cholesterol ratio of late endosome/lysosomal fraction (fraction 7 and 8) was expressed as the percentage of radioactivity of 20,0006g ppt.  Figure S8 obr quadruple mutants exhibit no abberation with receptor-mediated endocytosis. (A-D) Fluorescence images and the corresponding Nomarski micrographs of adult hermaphrodites of wild-type and obr quadruple mutants carrying the YP170::EGFP transgene. The YP170::EGFP fusion protein is transported like endogenous yolk, from intestine to oocyte via receptor-mediated endocytosis. In wild-type worms, the YP170::EGFP endocytosed two nearly full-grown oocytes of one gonad arm (A, arrows). In obr quadruple mutants (obr-1;2;3;4), YP170::EGFP is efficiently endocytosed and stored in oocytes in a similar manner to that in wild-type worms (C, arrows). Scale bars are 20 mm. In the proximal gonad, oocytes undergo maturation (A, arrowheads) and are ovulated into the sperm-containing spermatheca (A, asterisk) where they are fertilized. Fertilized eggs then move into the uterus (A, arrows). In control oocytes prior to fertilization, CAV-1::GFP is concentrated in intracellular vesicles and large ring-like cytoplasmic structures and localized weakly to the plasma membrane (A and B, arrowheads). Immediately after oocytes pass through the spermatheca and are fertilized, the amount of CAV-1::GFP on the cell surface rapidly increases, followed by its internalization and degradation. Newly fertilized embryos exhibited bright CAV-1::GFP fluorescence, initially at the cell surface (A and B, red arrows) and subsequently on internal membranes, but embryos beyond the 2-cell stage, approximately 90 minutes post fertilization, lacked visible fluorescence (A and B, white arrows).