Gli2 and Gli3 Localize to Cilia and Require the Intraflagellar Transport Protein Polaris for Processing and Function

Intraflagellar transport (IFT) proteins are essential for cilia assembly and have recently been associated with a number of developmental processes, such as left–right axis specification and limb and neural tube patterning. Genetic studies indicate that IFT proteins are required for Sonic hedgehog (Shh) signaling downstream of the Smoothened and Patched membrane proteins but upstream of the Glioma (Gli) transcription factors. However, the role that IFT proteins play in transduction of Shh signaling and the importance of cilia in this process remain unknown. Here we provide insights into the mechanism by which defects in an IFT protein, Tg737/Polaris, affect Shh signaling in the murine limb bud. Our data show that loss of Tg737 results in altered Gli3 processing that abrogates Gli3-mediated repression of Gli1 transcriptional activity. In contrast to the conclusions drawn from genetic analysis, the activity of Gli1 and truncated forms of Gli3 (Gli3R) are unaffected in Tg737 mutants at the molecular level, indicating that Tg737/Polaris is differentially involved in specific activities of the Gli proteins. Most important, a negative regulator of Shh signaling, Suppressor of fused, and the three full-length Gli transcription factors localize to the distal tip of cilia in addition to the nucleus. Thus, our data support a model where cilia have a direct role in Gli processing and Shh signal transduction.


Introduction
Cilia are microtubule-based organelles that protrude from the surface of most cells in the mammalian body and are formed through a conserved process termed intraflagellar transport (IFT) [1]. Polaris, the protein encoded by Tg737, is a core component of the mammalian IFT machinery and is required for the formation of all cilia and flagella [2,3]. Mice homozygous for the hypomorphic Tg737 orpk allele exhibit phenotypes in many tissues including the formation of cysts in the kidney, liver, and pancreas, hydrocephalus, and skeletal patterning defects that include extra molar teeth, cleft palate, and preaxial polydactyly [2,[4][5][6]. While Tg737 orpk mutants are viable, complete loss of Tg737 function in Tg737 D2-3b-gal mutants results in midgestation lethality, randomization of the left-right body axis, neural tube closure and patterning defects, and formation of eight to ten unpatterned digits per limb [3]. In Tg737 orpk and Tg737 D2-3b-gal mutant mice, cilia are severely malformed or absent, respectively, suggesting that this organelle is required for normal development and patterning of many tissues in the mammalian body [2,3,5].
The mammalian limb is patterned through the interaction of three main signaling centers [7]. The apical ectodermal ridge is necessary for proper limb outgrowth and proximaldistal length while the surface ectoderm regulates dorsalventral patterning. The zone of polarizing activity, located in the posterior mesenchyme, is involved in anterior-posterior patterning including the formation of five digits per limb. Sonic hedgehog (Shh) is secreted by cells in the zone of polarizing activity, and many polydactyl mutants in the mouse have been shown to have ectopic expression of either Shh or genes activated by Shh. The main targets of Shh signaling are the Glioma (Gli) transcription factors [8]. Three Gli transcription factors (Gli1, Gli2, and Gli3) have been identified in mammals. Gli3 exists as a full-length ''activator'' (Gli3A) that is proteolytically processed into a smaller form with repressor activity (Gli3R) in the absence of Shh ligand [9]. Binding of Shh to its receptor Patched1 (Ptch1) leads to the derepression of Smoothened and blocks processing of the Gli3 transcription factor. While mutations in Gli1 or Gli2 alone have no affect on digit patterning, loss of one or both alleles of Gli3 in Gli3 Xt-J mice is sufficient to produce ectopic digits [10,11]. The severe polydactyly in Gli3 Xt-J homozygous mutants is associated with ectopic expression of Shh and its target genes [12]; however, the loss of Shh in Shh À/À ;Gli3 Xt-J double mutants results in identical digit patterning defects as seen in Gli3 Xt-J mutants alone, leading to the hypothesis that a pentadactyl restraint is imposed on the limb by Shh counteraction of Gli3 repressor activity [13,14].
Another important component of the Shh signaling pathway involved in Gli protein regulation is Suppressor of fused (Sufu). Sufu is a negative regulator of Shh signaling that interacts with all three Gli proteins and mediates their nuclear export in the absence of Shh [15][16][17]. In Drosophila, Su(fu) is thought to link Ci (Gli homolog) to the ubiquitin proteasome required for conversion of Ci to the small repressor form, as well as retain unprocessed Ci in the cytoplasm in the absence of ligand; however, this has not yet been demonstrated for the mammalian pathway [8].
Previously, we demonstrated that partial disruption of IFT function in Tg737 orpk mutants results duplication of digit I, while complete loss of IFT in Tg737 D2-3b-gal mutants leads to the formation of up to ten unpatterned digits per limb [6]. Despite the formation of excess digits and a known involvement of Shh in ectopic digit formation in many mouse models [7], Shh expression is not altered in either Tg737 orpk or Tg737 D2-3b-gal mutants [6]. In addition, no alterations in Ptch1 expression in Tg737 orpk hypomorphic mutants is observed. Recent evidence from the labs of Anderson and Niswander has shown that IFT proteins, including Tg737/Polaris, are essential for Shh signaling in both neural tube and limb patterning at the level of Gli3 processing, although the connection between IFT and this signaling pathway remains enigmatic [18][19][20]. Previous models have speculated that there is a cytosolic role for the IFT proteins or that the cilia generate a signal affecting the activity of the Gli transcription factors. However, in this report we provide evidence supporting the hypothesis that there is a direct role for cilia in Shh signal transduction. Despite normal Shh expression in homozygous Tg737 D2-3b-gal mutant limbs [6], expression of the Shh downstream targets such as Ptch1 and Gli1 is lost. Furthermore, cells isolated from mutant limb buds are unable to respond to ShhN conditioned medium (ShhN-CM) in vitro. Even though mutant cells are unable to respond to ShhN-CM, the pathway can be activated by exogenously expressed Gli1; however, Gli2 and full-length Gli3 were found to be inactive in the absence of Polaris. Although full-length Gli3 was nonfunctional in Tg737 D2-3b-gal mutant cells, expression of a processed form of Gli3 (Gli3R) acts as a potent repressor of Gli1-mediated induction of Ptch1 expression. Additionally, the amount of full-length Gli3 was markedly increased in Tg737 D2-3b-gal mutant embryos relative to wild-type controls, suggesting that loss of Polaris results in inefficient Gli3 processing. Most importantly, the data indicate that all three full-length Gli proteins along with Sufu colocalize to the distal tips of cilia in primary limb bud cells. Together our data support a direct role for cilia in the mammalian hedgehog signaling pathway and raise the intriguing possibility that the tip of the cilium is a specialized domain in which proteolytic machinery is concentrated for processing and regulating the activity of Shh signal transduction.

Cilia Are Present on Both Ectoderm and Mesenchyme Cells of the Limb Bud
To determine whether cilia are present on the developing mouse limb bud, we conducted electron microscopic analysis of embryonic day 11.5 (E11.5) limb buds. Using transmission electron microscopy, cilia were found on the mesenchyme. These cilia have a 9 þ 0 microtubule structure, were frequently found in depressions in the cell membrane, and were always closely associated with the Golgi apparatus ( Figure 1A-1C). In addition, small vesicular structures were frequently detected near the base of the cilium. Using scanning electron microscopy, we also determined that most, if not all, ectodermal cells exhibit a single cilium ( Figure 1D and 1E).
To further confirm the presence of cilia in the limb bud, we conducted immunofluorescence analysis of frozen sections using anti-acetylated a-tubulin, which recognizes stabilized microtubules including the cilium axoneme, and anti-Polaris antiserum. The data indicate that Polaris concentrates at the base and distal tip of cilia on both ectodermal and mesenchymal cells as well as in a punctuate pattern overlapping that of acetylated a-tubulin in the axoneme ( Figure  1F-1H). In primary cultures of limb bud cells, cilia were found on most cells when visualized with anti-acetylated atubulin and anti-Polaris antibodies ( Figure 1H). In contrast, the cilia were completely absent from cells isolated from Tg737 D2-3b-gal mutants ( Figure 1I). Domains of stabilized microtubules were still present around the microtubule organizing center (MTOC) from which the cilia would have emerged.
The Hedgehog Signaling Pathway Is Repressed in Tg737 D2-3b-gal Mutants In agreement with previous data in the limb and neural tube [18][19][20], there was no significant expression of two downstream targets of Shh signaling, Ptch1 and Gli1, in Tg737 D2-3b-gal null mutant limb buds (Figure 2A and 2B). These data suggest that despite normal Shh expression in these mutants, Shh release or reception is impaired because of the loss of Polaris. These results confirm our assessment that the IFT mutant limb phenotype is not due to ectopic activation of the Shh pathway and that the phenotype in

Synopsis
Cilia are small projections extending from the surface of most cells. Research has shown that they are important in diseases such as cystic kidney diseases as well as during the development of many tissues including the limb. More recently, proteins such as Polaris, which is required to build cilia, have been demonstrated to be essential for the regulation of Sonic hedgehog (Shh) signaling, although the mechanism has remained elusive. Precise regulation of Shh signal transduction is important for the proper development of many tissues. Excessive activation of the Shh pathway results in severe developmental defects and has been implicated in certain types of cancer. In the limb, Shh signaling is involved in digit development, and excess signaling leads to the formation of extra digits. The main targets of Shh signaling are the Glioma (Gli) family of transcription factors, and Gli3 has been shown to be processed to a shortened repressor form when Shh signaling is repressed. The localization of the Gli transcription factors and Suppressor of fused, a protein involved in the regulation of Gli protein function, to cilia suggests that the cilia may be an important site for regulation of Shh signal transduction by modulating Gli protein function.

Cells Lacking Polaris Are Unable to Respond to ShhN
To test whether Polaris is required for Shh reception, we isolated cells from Tg737 D2-3b-gal mutant and wild-type limb buds (E11.5) and cultured the cells in ShhN-CM. The ability of the cells to respond to ShhN was determined by induction of Ptch1 and Gli1 expression using semi-quantitative reverse transcription PCR (RT-PCR). While robust response to ShhN-CM was seen in wild-type cells, cells lacking Tg737 showed no increase in the expression of Ptch1 or Gli1 relative to control treated cells ( Figure 2C). These data indicate that Polaris is required in Shh responding cells to activate the Shh signaling pathway in the presence of ligand.

Loss of Polaris Results in Altered Gli Activity and Processing
Genetic studies have indicated that IFT function is required for Shh signaling downstream of Ptch1, possibly at the level of Gli function [18][19][20]. To further explore the connection between Gli activity and Polaris, we used adenoviruses [21] to express the full-length Gli proteins in Tg737 null cells. Previous results have shown that ectopic expression of Gli1 and Gli2 can induce transcription of Shh target genes while Gli3 has been shown to inhibit Gli1mediated transcription [8,21,22]. As seen in wild-type cells, infection of Tg737 D2-3b-gal primary limb cells with full-length Gli1 resulted in increased transcription of Ptch1 compared to infection with green fluorescent protein (GFP)-only virus ( Figure 3A). This indicates that Polaris function is not required for Gli1-mediated pathway activation. However, infection of Tg737 D2-3b-gal primary limb bud cells with Gli2expressing virus failed to induce Ptch1 transcription ( Figure  3B) suggesting that Gli2 function requires the activity of Polaris. It is unclear at this time whether the loss of Gli2 function in Tg737 D2-3b-gal mutants is due to a requirement of Polaris for Gli2 stability or other post-translational regulation. As seen in previous studies, infection of cells with the full-length form of Gli3 was able to repress Gli1-mediated transcription when coexpressed in wild-type cells [22]. However, in cells lacking Polaris, full-length Gli3 failed to repress pathway activation by Gli1, as evidenced by increased Ptch1 expression ( Figure 3A).

Gli3 Processing Is Inhibited by Loss of Polaris
The above data raised the possibility that loss of Polaris impaired the conversion of the full-length Gli3 to the  truncated repressor form. To determine if this was the case, we examined the levels of full-length and processed forms of Gli3 in wild-type and Tg737 D2-3b-gal whole embryos (E11.5) by Western blot analysis using Gli3 antiserum (gift of B. Wang). In agreement with previously published results [19,20], there was a marked increase in the ratio of the full-length Gli3 to the processed form of Gli3R in Tg737 mutants ( Figure 3C), although some Gli3R is clearly evident. Together, these data suggest that Polaris is required for efficient processing of Gli3.
To determine if the loss of Gli3-mediated repression in Tg737 D2-3b-gal mutants was due to defects in processing of full-length Gli3 to the repressor form, we infected primary cells with a truncated form of Gli3 (Gli3R) and analyzed the effect on Gli1-mediated transcription. In both wild-type and Tg737 D2-3b-gal mutant cells, the processed form of Gli3R was able to function as a potent repressor of Gli1-induced transcription of Ptch1 ( Figure 3D). These results indicate that the loss of Gli3 activity observed with the full-length form is due to a defect in processing and not a loss of repressor activity or trafficking to the nucleus.

Partial Loss of Polaris Function Exacerbates the Phenotype of Gli3 Heterozygous Mutants
We predicated that if Polaris is required for proper Gli3 processing, partial loss of Polaris function as seen with the Tg737 orpk hypomorphic allele would exacerbate the phenotype of Gli3 heterozygous mice and cause a phenotype that is more reminiscent of Gli3 null mutants. To evaluate this possibility, we crossed Tg737 orpk/þ heterozygous mice with Gli3 Xt-J/þ ;Tg737 orpk/þ compound heterozygotes and correlated the resulting phenotypes with the genotypes of the embryos. Heterozygous Gli3 Xt-J/þ mice are viable and exhibit a single additional preaxial digit similar to that seen in homozygous Tg737 orpk/orpk mutants [6,12,23]. In contrast, homozygous Gli3 Xt-J/Xt-J mutants are nonviable and have 8-10 nonpatterned digits per limb, as is also seen in Tg737 D2-3b-gal null mutants [12]. Intriguingly, no viable Gli3 Xt-J/þ ;Tg737 orpk/orpk offspring were obtained (0/64 pups; seven litters). Analysis at earlier developmental stages indicated that the Gli3 Xt-J/þ ;Tg737 orpk/orpk mice die during gestation with severe developmental abnormalities including 6-9 digits per limb, exencephaly, abdominal closure defects, and edema (Figure 4A-4E; data not shown). These phenotypes are not characteristic of Gli3 Xt-J/þ heterozygous or Tg737 orpk/orpk homozygous mice alone but are seen in Gli3 Xt-J/Xt-J homozygous mutants.
While both Tg737 D2-3b-gal and Gli3 Xt-J mutant mice have similar digit patterning phenotypes, Tg737 D2-3b-gal mutants do not express Ptch1 or Gli1 in the posterior limb bud while in Gli3 Xt-J mutants the expression domains of Ptch1 or Gli1 are expanded. To determine if the limb patterning defects in Gli3 Xt-J/þ ;Tg737 orpk/orpk mutants were due to a loss of Shh responsiveness, as seen in Tg737 D2-3b-gal mutants, or more closely resembled the phenotype of Gli3 Xt-J mutants, we tested primary cells derived from these embryos for their ability to induce Gli1 expression in response to ShhN-CM. In contrast to Tg737 D2-3b-gal mutants, Gli3 Xt-J/þ ;Tg737 orpk/orpk cells responded to ShhN-CM with increased expression of Gli1 when analyzed by quantitative RT-PCR, although at reduced levels compared to wild-type cells ( Figure 4F). In agreement with the quantitative RT-PCR data, Gli1 expression was observed in the posterior region of all embryos by in situ hybridization (data not shown). No overt or consistent differences were evident in Gli3 Xt-J/þ or Gli3 Xt-J/þ ;Tg737 orpk/orpk embryos when compared to wild-type (data not shown). Together these data indicate that the Gli3 Xt-J/þ ;Tg737 orpk/orpk phenotype more closely resembles that of Gli3 homozygotes than that of Tg737 null mutants, which are nonresponsive to ShhN-CM.

Exogenously Expressed Components of the Shh Signaling Pathway Localize to the Cilia
While it is known that IFT is required for Shh signaling [18,19], it remains unclear whether this is due to a requirement for cilia in Shh pathway activation, production of a secondary signal by cilia, or a novel non-ciliary role for IFT. To begin distinguishing between these possibilities, we evaluated the subcellular localization of several key proteins involved in the Shh signaling pathway, including Gli1, Gli2, Gli3, Gli3R, and Sufu relative to cilia and the IFT protein Polaris.
In the case of the Gli1, Gli2, Gli3, and Gli3R proteins, localization was determined by infection of primary Tg737 D2-3b-gal mutant and wild-type limb bud cells with adenoviral vectors that express the full-length Gli proteins or the truncated Gli3R fused to GFP [21]. Infections were performed such that greater than 75% of the cells expressed GFP. For these studies, we focused on cells that had low levels of exogenous expression to minimize any effects that overexpression may have on protein localization. For all three full-length Gli proteins, expression was detected in the nucleus in cells that expressed high levels of GFP ( Figure 5; data not shown), as reported previously [22]. However, we also detected a small domain of GFP in all cells expressing the tagged protein that was located near the cilium axoneme as visualized with anti-acetylated a-tubulin antibodies ( Figure  5A-5C). The GFP signals failed to colocalize with c-tubulin (basal body marker), indicating that the Gli::GFP proteins do not localize to the basal body at the base of the cilia ( Figure  5E; data not shown). Rather, the GFP signal was found to colocalize with a subdomain of Polaris (Figure 5F and 5G; data not shown). The colocalization of Gli::GFP with a domain of Polaris, but not with c-tubulin, indicates that the fulllength Gli proteins concentrate at the tip of the cilium but not at the base. Treatment of infected cells with ShhN-CM did not alter the distribution of Gli1, Gli2, or Gli3 at the distal tips of cilia (data not shown). However, it may be difficult to assess any changes in localization since GFP is fused to the Cterminus of the Gli proteins. Thus, processing that occurs in the case of full-length Gli3 would remove the GFP tag and prevent visualization of the truncated N-terminal form of the protein that traffics to the nucleus.
In contrast to the localization observed for the three fulllength Gli proteins, Gli3R::GFP was detected predominantly in the nucleus. We could detect no GFP signal at the distal tip of cilia, suggesting that after processing, Gli3R is released from the cilia or that the cilia targeting domain is located in the C-terminus of Gli3 ( Figure 5D). These possibilities are currently being explored.
In Tg737 D2-3b-gal mutant cells that lack cilia, the Gli::GFP fusion proteins were seen in the nucleus, as observed in wildtype samples. Additionally, Gli1::GFP, Gli2::GFP, and Gli3::GFP were localized around the MTOC, where the cilia would have formed ( Figure 5H-5J). In contrast, Gli3R was present mainly in the nucleus and was not detected around the MTOC in either the wild-type or Tg737 D2-3b-gal mutant cells ( Figure 5D and 5K). The nuclear localization of the Gli::GFP proteins in Tg737 D2-3b-gal mutants suggests that Polaris is not required for nuclear import of the Gli transcription factors.

Endogenous Gli3 and Sufu Localize to the Tip of Cilia
To confirm the localization of GFP-tagged Gli3 at the tip of cilia, and to determine if this was the full-length Gli3 protein or only the GFP-tagged C-terminus, we conducted immuno- (F) Incubation of cells from Gli3 þ/þ ;Tg737 þ/þ , Gli3 Xt-J/þ ;Tg737 þ/þ , and Gli3 Xt-J/þ ;Tg737 orpk/orpk mutant mice with ShhN-CM resulted in increased expression of Gli1 when compared to cells from the same embryo treated with control medium, as determined by quantitative RT-PCR analysis. The results are reported for four littermates of the indicated genotypes. Each sample was analyzed in duplicate, and results are reported as the average fold increase. Unlike Tg737 D2-3b-gal (null) mutants, which are nonresponsive to ShhN-CM, the Gli3 Xt-J/þ ;Tg737 orpk/ orpk samples are able to respond and activate the pathway, indicating that the Gli3 Xt-J/þ ;Tg737 orpk/orpk phenotype does not resemble that of Tg737 D2-3b-gal (null) mutants but rather that of Gli3 Xt-J/ Xt-J . DOI: 10.1371/journal.pgen.0010053.g004 fluorescence analysis of endogenous Gli3 in noninfected primary limb cells using Gli3 antisera generated against the N-terminus of the protein ( Figure 6). The data indicate that, as seen with exogenously expressed Gli3::GFP, endogenous Gli3 was concentrated at the tip of cilia ( Figure 6A; data not shown). Since the Gli3 antiserum recognizes the N-terminus of Gli3, and GFP is fused to the C-terminus in Gli3::GFP virus, the data suggest that it is the full-length form of Gli3 that localizes to the cilium tip.
Since all three Gli proteins localize to the tip of the cilium and to the nucleus, and since Sufu has been shown to directly interact with the Gli proteins [17], we predicted that Sufu would also be present in these two regions of the cell. To explore this possibility, we analyzed the localization of endogenous Sufu by immunofluorescence in primary limb bud cultures. The data confirm that endogenous Sufu colocalized with endogenous Gli3 and with the Gli::GFP fusion proteins, and partially overlapped with Polaris ( Figure  6A and 6D; data not shown). It was also present adjacent to acetylated a-tubulin in the cilium axoneme and did not localize with c-tubulin at the basal body, again indicating that these proteins concentrate at the tip of the cilium ( Figure 6B and 6C). A low level of Sufu was also evident in the nucleus but was in a different plane of focus when the Sufu image for Figure 6 was captured. In addition, Sufu was detected in the cytosol; however, whereas the nuclear and cilium tip signals were blocked by preincubation with the immunizing peptide, the cytoplasmic signal remained unchanged ( Figure 6E and 6F), suggesting that it may be nonspecific.

Discussion
Cilia are expressed on many different cell types in the mammalian body. They are formed and maintained by a highly conserved process termed IFT, but they perform diverse functions on various cell types [1]. In mammals, cilia have been demonstrated to play a critical role in developmental processes-from left-right axis specification and skeletal patterning to normal kidney, pancreas, and liver physiology-as well as disease processes [2][3][4]6,24]. Recent data have demonstrated that the IFT proteins, which are necessary for cilia formation, are also required for proper limb and neural tube patterning [6,[18][19][20]. Furthermore, the IFT proteins have been shown to function as part of the Shh signal transduction pathway and in regulating Gli activity [19,20]. Here we show that Gli2 and full-length Gli3 function are disrupted in the Tg737 D2-3b-gal cilium mutants. In contrast, our data indicate that Gli1 and a processed form of Gli3 (Gli3R) are able to induce or repress the Shh pathway, respectively, regardless of the presence or absence of Polaris. Most important, all three full-length Gli::GFP proteins, as well as endogenous Gli3 and Sufu, localize to the distal tip of cilia in primary limb bud cell cultures, supporting a direct role for cilia in regulating Shh signal transduction.
Mice homozygous for the hypomorphic Tg737 orpk allele have preaxial polydactyly, but no alterations in expression of Shh or its downstream targets are evident [6]. Preaxial polydactyly is also seen in mice heterozygous for the Xt-J allele of Gli3 [10]. Interestingly, Gli3 Xt-J/þ ;Tg737 orpk/orpk mice develop multiple ectopic digits on all four limbs and die during gestation. This phenotype is reminiscent of both Gli3 Xt-J and Tg737 D2-3b-gal homozygous mutants. However, unlike cells from Tg737 D2-3b-gal mutants, cells from Gli3 Xt-J/þ ;Tg737 orpk/orpk mice are able to induce Gli1 transcription in response to ShhN-CM. These data suggest that the hypomorphic Tg737 orpk allele further disrupts Gli3 function and converts the Gli3 Xt-J/þ heterozygotes to a phenotype more similar to that seen in the Gli3 null mutants. Previous genetic studies on IFT mutant mice have indicated that IFT proteins function in Shh signal transduction at a step downstream from the membrane proteins Ptch and Smoothened and are required for all Gli function [18][19][20]. In contrast, our data suggest Polaris is required for specific Gli functions since Gli1 and truncated Gli3 (Gli3R) are active in the absence of Polaris while Gli2 and full-length Gli3 are not. These data suggest that the defects observed when mutant cells are infected with full-length Gli3 are likely due to loss of processing of Gli3 to form the repressor, but not loss of repressor activity. While processing of Gli2 remains controversial, Gli2 function is disrupted in Tg737 D2-3b-gal mice, suggesting that IFT or cilia are required for some aspect of Gli2 regulation or activity. This does not appear to involve translocation of the Gli proteins to the nucleus since they are all detectable in the nucleus of Tg737 D2-3b-gal mutant cells.
In Drosophila, Su(fu) is involved in the negative regulation of Ci (Gli homolog) transcriptional activity by sequestering it in the cytoplasm and targeting it for proteolytic processing to produce a transcriptional repressor [8]. Whether the role of Sufu in targeting Gli proteins for proteolytic processing is conserved in mammals has yet to be determined. Mammalian Sufu has been shown to interact with all three Gli proteins through a conserved SYGH motif in the N-terminus of the Gli proteins in addition to a region in the C-terminus of Gli1, and negatively regulates Gli1 transcriptional activity [16,17]. The colocalization of Sufu and the Gli proteins to the tip of the cilium, along with a requirement for IFT in proper Gli3 processing, suggests that mammalian Sufu may have a similar role in Gli regulation and, furthermore, that proteolytic processing may occur at the tip of the cilium.
Huangfu et al. [18] proposed two possible models for how IFT may regulate Shh signaling, one suggesting the involvement of a cilia-derived signal that is required for Shh pathway activation and a second model in which IFT has two separate functions, one in ciliogenesis and a second one in intracellular transport. While testing these models is hindered by our inability to specifically disrupt cilia formation without also perturbing IFT, the data presented here support a direct role for cilia in Shh pathway regulation. This is based on the localization of multiple components of the Shh pathway in the cilia of wild-type cells, and on altered Gli3 processing and impaired Gli2 function detected in cells lacking this organelle. While we cannot conclusively rule out a nonciliary function of IFT, we propose that IFT functions to direct and concentrate the Gli proteins, Sufu, and possibly the proteolytic machinery needed for efficient processing of the Gli proteins to a domain located at the distal tips of cilia. In the absence of the cilium, the Gli proteins localize diffusely around the basal body region and fail to undergo normal processing, resulting in their impaired activity.

Materials and Methods
Mouse strains and methods. Tg737 D2-3b-gal , Tg737 orpk , and Gli3 Xt-J mice have been previously described [3,4,23]. Tg737 D2-3b-gal mice were maintained on a mixed FVB 3 BALB/c background and genotyped as described [3]. Tg737 orpk mice were maintained on an FVB background and genotyped as described [3]. Gli3 Xt-J mice were maintained on a C57BL/6 background and were genotyped as described [3,25]. Analysis of phenotypes for Gli3 Xt-J ;Tg737 orpk mice was performed on pups or embryos from F 1 intercrosses. For staged embryos, noon of the day of the vaginal plug appeared was considered E0.5.
Skeletal stains were performed as described [29]. Cell culture. ShhN-CM and vector-only control conditioned medium were generated as previously described [30]. For induction assays, cells were cultured in a 1:1 mixture of conditioned medium and DMEM þ 15% FBS overnight prior to RNA isolation.
Embryos were isolated and identified by phenotype (Tg737 D2-3b-gal mutants) or by PCR using DNA isolated from yolk sacs. Limb buds for cell culture experiments were removed and treated with 0.25% trypsin in PBS for 15 min at room temperature. Following trypsin treatment, cells were mechanically dissociated and FBS was added to 10%. Cells were collected by centrifugation and plated with DMEM þ 15% FBS.
Gli1::GFP, Gli2::GFP, and Gli3::GFP adenovirus constructs encoding C-terminal GFP fusions have been previously described [21]. The Gli3R::GFP adenovirus was generated by replacing the full-length cDNA in the Gli3::GFP vector with the coding region corresponding to amino acids 1-677. This truncated form of Gli3 (Gli3R) has been previously shown to act as a constitutive repressor of Shh signaling [31]. All infections were optimized to produce greater than 75% infection and were done at least three times. To determine if expression levels were consistent between samples, we examined the level of expression of the Gli::GFP genes by RT-PCR using primers specific for the GFP coding region. Similar levels of expression were seen in wild-type and mutant samples under the same infection conditions. All localization data shown are representative of the pattern observed in the majority of the cells from all experiments. An identical pattern of localization was observed in the IMCD mouse kidney cell line. No specific localization of GFP was found for cells infected with GFP control virus only.
RNA isolation and RT-PCR. RNA was isolated using TRIzol (Invitrogen, Carlsbad, California, United States) according to the manufacturer's instructions. Reverse transcription was performed using SuperScript II reverse transcriptase (Invitrogen) according to the manufacturer's instructions. Equal amounts of cDNA were used as templates for PCR with Taq Polymerase (Brinkman Instruments, Westbury, New York, United States) according to the manufacturer's instructions. Relative expression levels were calculated by comparing the intensity of the Ptch1 or Gli1 PCR product to the actin PCR product in the same reaction using LabWorks 4.0 software (UVP, Upland, California, United States). Primer sequences are available upon request.
Quantitative RT-PCR measurement was performed using the SmartCycler machine (Cepheid, Sunnyvale, California, United States). The TaqMan primer and probe sets, for Gli1 and 18S rRNA (TaqMan Assays-on-Demand Products), were purchased from Applied Biosystems (Foster City, California, United States). The 18S rRNA gene was used as an internal control. The threshold cycle (C T ) for Gli1 was first normalized to the corresponding 18S rRNA C T . Relative fold differences were then determined using the 2 (-D,D[C T ]) method [32] by comparing the expression levels in ShhN-CM-induced cells to their vector conditioned medium controls. No significant difference in basal Gli1 expression in vector conditioned medium-treated cells was evident between wild-type and Gli3 Xt-J/þ samples.
Immunofluorescence. For analysis of cilia in vivo, limb buds were dissected from wild-type embryos (E10.5), embedded in OCT, and snap frozen. Sections of 20 lm were cut and stained as previously described [33] using 0.2% Triton X-100 for permeabilization. Cultured primary cells were fixed, permeabilized, and stained using an identical procedure. Anti-Polaris polyclonal antibody was generated by Sigma-Genosys (The Woodlands, Texas, United States) and screened for specificity by Western blot analysis and immunofluorescence. The antiserum recognized a single band of the correct size in wild-type samples by Western blot analysis; this band was absent in Tg737 D2-3b-gal samples. Only faint nuclear staining was observed in Tg737 D2-3b-gal primary cells by immunofluorescence, whereas localization to cilia was additionally observed in wild-type samples. Sufu antibody and blocking peptide were obtained from Santa Cruz Biotechnology (Santa Cruz, California, United States). Identical staining with the Sufu antibody was seen in the IMCD mouse kidney cell line. Affinity-purified anti-Gli3 antiserum was provided by B. Wang and used as previously described [9]. Acetylated a-tubulin and c-tubulin antibodies were obtained from Sigma (St. Louis, Missouri, United States). Gli::GFP fusion proteins were visualized using the GFP tag encoded by the adenovirus constructs. Nuclei were stained with Hoechst 33258 (Sigma).
All fluorescence imaging was performed on a Nikon (Tokyo, Japan) TE200 Eclipse inverted epifluorescence microscope equipped with a CoolSnap HQ cooled CCD camera (Roper Scientific, Trenton, New Jersey, United States) and MetaMorph imaging software (Molecular Devices, Downington, Pennsylvania, United States). All filters and shutters were computer driven.
Electron microscopy. Wild-type E11.5 embryos were isolated in PBS and fixed in 2.5% gluteraldehyde in 0.1 M cacodylate buffer for 90 min at room temperature. Samples were washed in three changes of 0.1 M cacodylate buffer, fixed in 1% OsO 4 for 60 min at room temperature, washed in thee changes of 0.1 M cacodylate buffer, and dehydrated through a graded ethanol series. Samples were processed for scanning or transmission electron microscopy by the University of Alabama at Birmingham High Resolution Imaging Facility using standard procedures. Scanning electron microscopy samples were imaged on an ISI SX-40 scanning electron microscope (Topcon Technologies, Paramus, New Jersey, United States). Transmission electron microscopy sections were imaged on a Zeiss (Oberkochen, Germany) EM 10C transmission electron microscope.