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Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness

Fig 7

Transcriptomics and metabolomics assays.

Differentially expressed KEGG pathways and metabolites from wandering third instar female larvae fed the 1:2 P:C and 1:16 P:C food. Red indicates elevated in Dahomey, blue elevated in Alstonville with darker colours representing smaller FDRs. Detailed FDRs are shown in S3 Table. (A) The differentially expressed KEGG pathways observed in RNA-seq profiling for the 1:2 P:C diet (n = 4 biological rep/mitotype). MXC P450 is Methoxychlor-Cytochrome P450, C P450 is Cytochrome P450, A & A is Ascorbate and Aldarate, P & C is Porphyrin and Chlorophyll, P & G is Pentose and Gluconate, ER is endoplasmic reticulum. (B) The differentially expressed KEGG pathways observed in RNA-seq profiling for the 1:16 P:C diet (n = 4 biological rep/mitotype). NER is nucleotide excision repair, BER is base excision repair, FoxO is Forkhead box, HR is homologous recombination, Pyrimidine is pyrimidine metabolism, OXPHOS is oxidative phosphorylation, TCA is tricarboxylic acid, Glycerophospholipid is glycerophospholipid metabolism, and Carbon is carbon metabolism. Detailed FDRs are shown in S5 Table. (C) Differentially abundant metabolites observed in GC/MS profiling (n = 5 biological rep/mitotype) for the 1:2 P:C diet. (D) Differentially abundant metabolites observed in GC/MS profiling (n = 5 biological rep/mitotype) for the 1:16 P:C diet.

Fig 7

doi: https://doi.org/10.1371/journal.pgen.1007735.g007