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Genotype to phenotype: Diet-by-mitochondrial DNA haplotype interactions drive metabolic flexibility and organismal fitness

Fig 4

The V161L ND4 amino acid change in complex I of Dahomey mtDNA influenced mitochondrial functions.

(A) The activity of complex I is higher in Alstonville larvae (n = 7 biological rep/mitotype/diet- with two failed reactions removed). (B) Oxygen consumption rate with complex I substrates is higher in Alstonville larvae (n = 6 biological rep/mitotype for 1:2 P:C diet; n = 9 biological rep/mitotype for 1:16 P:C diet). (C) Representative western blot showing reduced levels of complex I (CI) in Dahomey compared to Alstonville on both diets. There were no obvious differences in complex V (CV) or actin protein levels. Repeat gels showed all CI bands were the same size. (D) Native protein gel showing reduced activity of the peripheral arm of complex I (CI) and the supercomplex (SC) in larvae harbouring Dahomey mtDNA on both diets. Bars (meanĀ± s.e.m). t-tests between mitotypes * p< 0.05, ** p< 0.01 (see text).

Fig 4