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Sequential induction of three recombination directionality factors directs assembly of tripartite integrative and conjugative elements

Fig 4

Transcriptional regulation of rdfG and rdfM by RdfS.

β-galactosidase assays [67] were performed for (A) WSM1271 and (B) R7ANS carrying either control vector pPR3 or pPR3-rdfS (constitutively expressing rdfS) together with one of three RDF promoter-lacZ fusion constructs cloned into the pSDz vector. Assays were performed with six biological replicates and mean β-galactosidase activity values (Relative Fluorescent Units/s/OD600) were compared by Bonferroni adjusted student’s t-tests. SD is denoted by error bars.

Fig 4