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Sequential induction of three recombination directionality factors directs assembly of tripartite integrative and conjugative elements

Fig 2

qPCR measurement of excisive ICEMcSym1271 recombination.

Measurements represent the mean percentage of WSM1271 chromosomes in stationary-phase cultures harbouring each excisive Int-mediated recombination product (attBS, attPS, attBG, attPG, attPM, and attPM) determined by qPCR [19]. Where appropriate, plasmids carried by WSM1271 (here abbreviated as 1271) are listed in brackets after the strain name (see Table 3 for a description of plasmids). Values for each of the assay types attBS, attPS, attBG, attPG, attPM, and attPM site were individually compared between strains within the same panel (panel A, B, or C) using ANOVA and Fisher’s LSD test controlling for type I error using the Bonferroni adjustment. Groups of values from the same assay type and in the same panel that are not significantly different from each other have the same letter (a, b, c, d, e, f or g) indicated above. Expression from the IPTG inducible promoter of pSDz constructs were not induced with IPTG as they exhibit leaky expression without induction in TY medium used for assays. (A) Involvement of rdfG and rdfM in excisive recombination. (B) Quorum-sensing induction of excisive recombination. (C) Involvement of rdfS in excisive recombination.

Fig 2