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The Gene Regulatory Network of Lens Induction Is Wired through Meis-Dependent Shadow Enhancers of Pax6

Fig 2

The expression of lens placode-specific transcription factors is disturbed in Meis1/Meis2 double mutants.

(A-H‘) Cryosections from E10.0 control and Le-Cre;Meis1-/-;Meis2f/f embryos stained with antibody as indicated and nuclei counterstained with DAPI. (B, B‘) Pax6 is not detected in lens surface ectoderm of Le-Cre;Meis1-/-;Meis2f/f embryos (arrowheads) and (D, D‘) expression of the lens differentiation gene Foxe3 is not initiated. (F, F‘) Sox2 is detected in PLE of Meis1/Meis2 double mutants, althouth it failed to thicken. (H, H‘) Finally, expression of Six3 is decreased compared to control. Lens placode (LP) is indicated by dashed line. (A‘-H‘) For clearer examination, lens placode or corresponding lens surface ectoderm region is magnified and shown separately.

Fig 2