Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress
(A) Representative pictures of 17-day old seedlings of Col-0 wild type and crk2. Complementation of crk2 with 35S::CRK2-CDS:YFP rescued the growth defect of the mutant. Plants were grown under the following conditions: 250 μmol m-2 s-1 light intensity under 12 h-day length (day: 23°C, 70% relative humidity; night: 18°C, 90% relative humidity). Bar = 1 cm. Pictures are representative of three independent experiments. (B) A selection of crk mutant lines showing earlier senescence compared to Col-0 wild type. Results are means ± SE (n = 8). (C) Several crk mutants flowered earlier compared to wild type while crk2 flowered later. Results are means ± SE (n = 8). (D) Time course analysis of endosperm rupture showed delayed germination in several crk mutants compared to wild type. Results represent means from three independent biological experiments (n = 30). Testa and endosperm rupture were assessed every 5 hours up to 51 hours of imbibition. A seed was considered as germinated when the radicle protruded through both envelopes. (E) Several crk mutants exhibit a lower pavement cell density (number of pavement cells / mm2) in cotyledons. Results are means ± SE (n = 15). (F) Three crks showed slightly longer roots compared to wild type (measured eight days after stratification). Results are means ± SE (n = 16). (B-F) Differences between mutants and Col-0 wild type were compared and analysed using one-way-ANOVA (post hoc Dunnett, asterisks indicate statistical significance at *P<0.05, **P<0.01 and ***P<0.001) for (B, C, E) and linear model with single step p-value adjustment (F). All experiments were repeated three times with similar results.