The Genome and Development-Dependent Transcriptomes of Pyronema confluens: A Window into Fungal Evolution
A. Multiple alignment of the CBM_14 domains of proteins from P. confluens, several Eurotiales and an insect. In addition to the P. confluens proteins, the following proteins were used for Clustal X analysis: A. gambiae, Anopheles gambiae sp|O76217.2; A. nidulans, Aspergillus nidulans ANID_00499 from the A. nidulans genome project http://www.broadinstitute.org/annotation/genome/aspergillus_group/MultiHome.html; A. niger, Aspergillus niger ref|XP_001397263.1; N. fischeri, Neosartorya fischeri ref|XP_001266454.1; P. chrysog., Penicillium chrysogenum ref|XP_002561001.1. The conserved cysteine residues are indicated by green arrowheads above the sequence. B. Quantitative real time PCR analysis of selected CBM_14 domain-encoding genes from P. confluens. Expression was analyzed for each gene in two independent biological replicates for the four conditions LL, DD, LLK and DDK (light and darkness in surface culture and submerged culture). Sexual development is only possible in condition LL. The graph shows mean and standard deviation (for better visualization, standard deviations for negative expression ratios are shown in the negative instead of the positive direction). Expression ratios were calculated to address the question if a gene is differentially regulated during sexual development (i.e. in LL/DD and in LL/LLK), or regulated by light (i.e. in LL/DD and LLK/DDK) or regulated by surface versus submerged growth (i.e. LL/LLK and DD/DDK).