Identification of Genes That Promote or Antagonize Somatic Homolog Pairing Using a High-Throughput FISH–Based Screen
a, Rank-order plot of the percentage ± SD of large nuclei. A nuclei was considered large if its volume was at or greater than the 95th percentile volume of control cells. X-axis denotes the RNAi target. P values were determined by an unpaired t test. Inset, the frequency of single-signal nuclei was plotted against the frequency of large nuclei. The coefficient of determination R2 is a measure of how well the data fit a linear regression, with values close to or exactly one representing a perfect fit. As R2 = 0.004, there is no significant correlation between the percentages of paired nuclei and large nuclei. A minimum number of 250 nuclei were scored for each dsRNA. b, The number of FISH signals was plotted against the volume of each nucleus following borr and scra RNAi. No correlation was found between the degree of unpairing (number of FISH signals) and the size of the nuclei.