Adaptive Evolution of the Lactose Utilization Network in Experimentally Evolved Populations of Escherichia coli
A) Growth curves for the ancestor (green), and reconstructed lacI (blue) and lacO1 (purple) mutants. Conditions used were: Lac→Glu, Glu→Lac and G+L→G+L, where the sugars indicate pre-conditioning and measurement environments, respectively. These transitions correspond to those present in the G/L and G+L evolution environments. OD values are plotted on a log10 scaled axis. B) LacZ expression time course for the ancestor and the ancestor with lacI and lacO1 mutations during growth in the G+L evolution environment. Values are the average of two independent replicates with standard deviation shown.