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A Comprehensive Genome-Wide Map of Autonomously Replicating Sequences in a Naive Genome

Figure 1

Isolation and characterization of K. lactis ARSs.

(A) Schematic of the ARS screen in K. lactis. (B) Schematic for the iterative predict-and-verify approach for identifying genomic ARSs. (C) Logos of KlACS [66]. Top panel, KlACS based on the initial 69 KlARSs. Bottom panel, KlACS based on 148 verified KlARSs. Arrows indicate an inverted repeat motif centered about an axis of symmetry marked by a vertical line. (D) An extended 50 bp motif of the S. cerevisiae ACS motif is shown in comparison with the KlACS.

Figure 1