De novo Assembly of a 40 Mb Eukaryotic Genome from Short Sequence Reads: Sordaria macrospora, a Model Organism for Fungal Morphogenesis
(A) Segregation of the ascospore-color mutant pam2 from a cross; wild type (black ascospores) by pam2 (yellow ascospores). Arrow points to a gene conversion indicated by two black and six yellow ascospores. (B–D) Meiotic prophase. Chromosome axes are stained by the cohesin-associated Spo76/Pds5 protein tagged with GFP. (B) Prophase nucleus of a spo11 null mutant: the 14 chromosomes do not align or synapse and this asynaptic status is seen from leptotene through pachytene. (C, D) Pachytene nucleus from wild-type Sordaria: the seven bivalents are differentiated by their size (D). Chromosome 2 (yellow), which bears the nucleolar organizing region, is attached to the nucleolus (nu). (E) The seven bivalents at late diplotene, stained by DAPI. Note the difference in size when compared to the pachytene nucleus. Bar (B–E) = 5 µm. (F) An EGFP-HEX1 fusion protein localizes to Woronin bodies. Bar = 10 µm. (G) The GFP-tagged developmental protein PRO41 localizes to the endoplasmic reticulum. Plasma membrane stained with FM4-64. Bar = 10 µm. (H, I) In a young protoperithecium (H), the GFP-tagged developmentally induced protein APP accumulates (I). Bar = 20 µm.