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A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells

Figure 10

Variations in the mappable ASE loci with in vitro iPS differentiation.

iPS cells (A), retinoic acid (RA)-treated iPS cells (B), and embryoid bodies (C) were compared to another iPS clone from the same individual pair-wise. Two different types of statistical threshold were used to categorize ‘mappable’ ASE loci. Mono-allelic expression is defined as having an allelic preference of >90%. N is the number of observation from cDNA samples. Retinoic acid was added directly to newly suspended iPS colonies growing on a low-attachment plate for 12 hours. Embryoid bodies were cultured the same way without retinoic acid for 7 days.

Figure 10