A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells
Variations in the mappable ASE loci with in vitro iPS differentiation.
iPS cells (A), retinoic acid (RA)-treated iPS cells (B), and embryoid bodies (C) were compared to another iPS clone from the same individual pair-wise. Two different types of statistical threshold were used to categorize ‘mappable’ ASE loci. Mono-allelic expression is defined as having an allelic preference of >90%. N is the number of observation from cDNA samples. Retinoic acid was added directly to newly suspended iPS colonies growing on a low-attachment plate for 12 hours. Embryoid bodies were cultured the same way without retinoic acid for 7 days.