A Robust Approach to Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells
Figure 1
PGP induced pluripotent stem cells.
(A) We derived 2–3 iPS cell lines from two biopsy sites in each individual. iPS cells expressed molecular pluripotency markers (SSEA4, SSEA3, Tra1-60, Tra1-81, NANOG and OCT4) and stained for the alkaline phosphatase activity. (B) When injected into immune-deficient mice, iPS cells formed a teratoma, containing normal tissues from all three germ layers, including respiratory epithelium (endoderm), bone (mesoderm) and neuroectoderm (ectoderm).