The Drosophila foraging Gene Mediates Adult Plasticity and Gene–Environment Interactions in Behaviour, Metabolites, and Gene Expression in Response to Food Deprivation
Flies carrying different for alleles crossed to mutants of the positive regulators (A) InR and (B) Dp110 (dashed curves) show almost none of the normal food-deprived rover-sitter food-leaving difference (compare to Figure 1 and solid balancer curves in this figure). In these quantitative complementation crosses, the food leaving behaviour of FD for;mutant transheterozygotes is compared to the FD for;Balancer transheterozygote controls. The difference in food leaving between the control balancer and mutant cross depends significantly on foraging allele, demonstrating interaction between the mutant gene and for. p(Interaction) = 0.012 (InR), p = 0.046 (Dp110). Data is arcsine transformed means±1 standard error for trials on n days (n = 11 for InR and Dp110). Behaviour assays were performed on FD flies as described in Figure 1 and Methods. Full ANOVA statistics are in Table S5.