Conditional knockout mice for the distal appendage protein CEP164 reveal its essential roles in airway multiciliated cell differentiation
Fig 5
CEP164 regulates small vesicle recruitment to basal bodies in multiciliated cells.
(A) TEM images of multiciliated cells from CEP164fl/fl and FOXJ1-Cre;CEP164fl/fl adult tracheas. Asterisks depict multiple cytoplasmic basal bodies in FOXJ1-Cre;CEP164fl/fl trachea. Scale bar, 500 nm. (B) TEM images of cross sections through the apical region of multiciliated cells from ALId14 MTECs derived from CEP164fl/fl and FOXJ1-Cre;CEP164fl/fl adult tracheas. Asterisks depict multiple cytoplasmic, misoriented basal bodies in FOXJ1-Cre;CEP164fl/fl multiciliated cells. Scale bars, 500 nm. (C) TEM images of CEP164fl/fl and FOXJ1-Cre;CEP164fl/fl P8 tracheas subjected to ex vivo culture in the presence of taxol to enrich vesicle-bound basal bodies. Arrowheads denote vesicles attached to the distal end of the cytoplasmic centriole in control CEP164fl/fl samples. Scale bar, 200 nm.