Fig 1.
Schematic of the Neurospora circadian clock in dark and light.
Fig 2.
Temporal expression profiles of reporter genes in DD and LD.
Luciferase activity under the control of the indicated promoters in WT (left panels) and Δvvd (right panels). The solid line represents the average of 30 measurements from three independent experiments. The shaded areas correspond to the standard deviation, SD. Yellow vertical boxes and grey areas indicate 12h light periods and dark periods, respectively.
Fig 3.
Model fitted to reporter gene expression in DD and LD.
Fig 4.
Maturation of inactive FRQ to active FFC is crucial for circadian rhythmicity.
(A) Maturation of FRQ to FFC was modelled by a linear chain of 6 generic steps. The impact of the number of steps on frq expression levels and rhythm is shown. (B) Delay between newly synthesized FRQ and assembled FRH (left panel) and abundance of all unassembled FRQ-species versus assembled FRH (right panel).
Fig 5.
Modelling the response of frq and vvd promoters to light-pulses of different intensity.
WT (left panels) and Δvvd (right panels) were exposed at t = 7 min to a virtual LP. (A) Modeled response of frq and vvd to virtual LPs of the indicated intensities. (B) Plot of peak expression levels of frq and vvd versus LP intensity. Simulated LP intensities: 0; 0.005; 0.005; 0.05; 0.5 and 5.0 arbitrary units (a.u.).