Table 1.
The dilution rates, calculated growth rates (Eq (3)), steady state concentrations of cells, metabolite exchange rates (Eq (4)) and carbon recovery.
Table 2.
R2 and median relative error (Median RE) of the experimental and predicted fluxes with (+mATP) or without mATP (-mATP) as constraint.
Data is shown for biomass equation R_biomass_cho.
Fig 1.
Experimental vs. predicted growth rates (A) and intracellular fluxes (B).
Data is shown for biomass equation R_biomass_cho as the objective function. RE—relative error. The legend in panel (A) indicates the publication and the used CHO cell line (if the information was available). Empty symbols indicate non-producers.
Fig 2.
Estimated mATP values and their effect on flux prediction accuracy.
(A) Computationally estimated mATP values for different datasets with two biomass equations. (B) R2 values from linear fits of experimental and predicted intracellular fluxes without (-mATP) or with mATP (+mATP) as constraint. The legend indicates the publication and the used CHO cell line (if the information was available). Empty symbols indicate non-producers.
Fig 3.
Experimental vs. predicted growth rates (A) and intracellular fluxes (B) after the addition of mATP as constraint.
Results are shown for R_biomass_cho as the objective function. RE—relative error. The legend in panel (A) indicates the publication and the used CHO cell line (if the information was available). Empty symbols indicate non-producers.
Fig 4.
Experimental vs. predicted fluxes using minimization of glucose uptake rate as the objective function.
(A) Experimental vs. predicted minimal glucose uptake rate. (B) Experimental vs. predicted intracellular fluxes. Results are shown for R_biomass_cho as the biomass reaction. RE—relative error. The legend in panel (A) indicates the publication and the used CHO cell line (if the information was available). Empty symbols indicate non-producers.
Fig 5.
The experimental exchange rates of glucose (A), lactate (B), glutamine (C) and ammonium (D) increase with increasing growth rate.
The shaded areas represent 95% confidence intervals. The triangle points in magenta color are the dilution rates that had unusually high cell concentration in steady state (see S7 Fig).
Fig 6.
Total energy production at different growth rates (as indicated in Table 1).
The black line is a linear fit and the intercept represents energy consumption at zero growth rate. The magenta triangles are dilution rates that had unusually high cell concentration in steady state (see S7 Fig). The shaded area represents 95% confidence interval.