Between-tumor and within-tumor heterogeneity in invasive potential
(A) Histogram of total Keratin 14 (K14) expression per organoid, calculated as the sum of the K14 intensity on a [0, 1] scale for pixels within the organoid divided by the total number of image pixels. (B) Histogram of mean K14 expression, calculated as the sum of the K14 intensity divided by the area of the organoid in pixels. The organoid size is less than the image size, and therefore the mean K14 is greater than the total K14. Both the total and mean were rank-normalized to generate uniform distributions for robust statistical analysis.