Between-tumor and within-tumor heterogeneity in invasive potential
Organoid boundaries are shown for 823 organoids generated from 52 breast tumors and imaged after six days of growth in 3D culture. Each column corresponds to organoids from a single tumor, denoted by an identifier underneath the column. Organoid boundaries were converted to a quantitative spectral power phenotype, represented by a false color map from blue (non-invasive) to red (highly invasive). For each tumor, organoids are stacked from less invasive to more invasive as characterized by the spectral power. Tumors are then arranged from left to right based on the median organoid invasiveness. Differences in numbers of organoids per tumor are from constraints on experimental capacity rather than biological differences between tumors. Heterogeneity is observed on both the horizontal axis (between-tumor variation) and the vertical axis (within-tumor variation).