PhysiCell: An open source physics-based cell simulator for 3-D multicellular systems
Videos are available at S1 and S2 Videos. Legend: Ki67+ cells are green before mitosis (K1) and magenta afterwards (K2). Pale blue cells are Ki67- (Q), dead cells are red (apoptotic) and brown (necrotic), and nuclei are dark blue. Bottom: Hanging drop spheroid experiment (HCC827 non-small cell lung carcinoma) showing a similar necrotic core microstructure. PhysiCell is the first simulation to predict this structure arising from cell-scale mechanical interactions. Image courtesy Mumenthaler lab, Lawrence J. Ellison Center for Transformative Medicine, University of Southern California.