A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation
Equimolar mixtures of Cas9 and crRNA (concentrations shown) were pre-incubated for 10 minutes, followed by the addition of target DNA and measuring the amount of cleaved DNA. Normalized cleaved DNA measurements (orange circles) using 25 nM negatively supercoiled plasmid DNA are compared to normalized model-calculated amounts of cleaved DNA (lines). Data points represent single measurements from Sternberg et al. .