An Ovol2-Zeb1 Mutual Inhibitory Circuit Governs Bidirectional and Multi-step Transition between Epithelial and Mesenchymal States
A) Zeb1 binds to the Ovol2 promoter in mouse mammary epithelial cells. Top, diagram showing the presence of Zeb1 consensus motifs (triangles) in Ovol2 promoter. Bottom, ChIP-PCR using primers for the upstream (left) and downstream sites (right). B) RT-quantitative PCR analysis showing that OVOL2 and ZEB1 overexpression in MCF10A cells results in decreased level of Zeb1 and Ovol2 transcripts, respectively TSS, transcription start site.