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OpenCyto: An Open Source Infrastructure for Scalable, Robust, Reproducible, and Automated, End-to-End Flow Cytometry Data Analysis

Figure 4

Example of OpenCyto automated gates on the perforin channel for CD8+ T-cells for six randomly selected samples from the HVTN 080 ICS data set.

The perforin marker exhibits staining variability as evidenced by the varying width and position of the negative peak and was not gated by the manual template-gating approach. Despite this variability, OpenCyto data-driven automated gating is able to identify a reasonable threshold for perforin positive cells.

Figure 4