Rational Engineering of Enzyme Allosteric Regulation through Sequence Evolution Analysis
(A) Structure of the E.coli FBPase (PDB code 2Q8M). Catalytic site (21 residues) and allosteric site (27 residues) residues were defined as amino acid residues within 6 Å of the substrate, and represented in red and cyan colors, respectively. (B) Distributions of conservation scores for catalytic, allosteric, and surface residues. The statistical significance (P-value) was measured by the Mann-Whitney U test.