Adhesion Failures Determine the Pattern of Choroidal Neovascularization in the Eye: A Computer Simulation Study
A) Total number of stalk cells vs. time. B) Total number of stalk cells confined in the sub-RPE space vs. time. C) Total number of stalk cells in contact with the POS (stalk cells in the sub-retinal space) vs. time. D) Total number of RPE cells vs. time. E) Total contact area between RPE cells and BrM vs. time. F) Total contact area between POS cells and BrM vs. time. The different colors represent the dynamics of 10 simulation replicas of the adhesion scenario (RRl = 3, RRp = 3, RBl = 2, RBp = 2, ROl = 3) (Table S5, adhesion scenario ID: 38). (A, B) CNV initiates in 9 out of 10 simulation replicas. All develop Early Type 1 CNV. CNV remains confined in the sub-RPE space during one simulated year (Stable Type 1 CNV). A Fully developed sub-RPE capillary network contains about 45 stalk cells (∼3000 cells/mm2). In 5 simulation replicas a few stalk cells die during the simulated year due to lack of RPE-derived VEGF-A. (C) Stalk cells have minimal contact with the POS. (D, E) The RPE remains viable and its total contact area with BrM decreases as stalk cells proliferate. (F) The POS never contacts BrM, indicating that the RPE does not develop any holes.