Binding Leverage as a Molecular Basis for Allosteric Regulation
(A) The hexameric structure of BDGH with ligand atoms drawn as spheres. Red ligands are NADP, blue GTP and green ADP. The individual chains are colored yellow, orange and white within each trimer. 1nr7 was used for protein coordinates, and NADP, GTP and ADP were taken from PDB entries 1hwz and 1nqt. The antenna helices of three of the chains are indicated with arrows. (B) Slightly rotated view showing the active and both allosteric sites, colored according to fi(0.25). (C) When more probe locations are included in the analysis, latent sites emerge, as exemplified here by fi(0.41). (D) ROC curves.