Prediction of Protein Binding Regions in Disordered Proteins
Top: Number of atomic contacts (green) and prediction output (blue) and for the N-terminal binding region of human p27. “D1”and “D2” denote the two strongly interacting domains (red boxes) and “LH” denotes the weakly interacting linker domain between them (yellow box). Bottom: Crystal structure of human p27 (red and yellow) complexed with CDK2 (magenta) and Cyclin A (blue) (PDB ID: 1jsu ). Red parts denote regions that are predicted to bind by the predictor. These regions correspond to the experimentally verified strongly binding regions of p27. The figure was generated by PyMOL.