Ligand discrimination in immune cells: Signal processing insights into immune dysfunction in ER+ breast cancer
Fig 2
Illustration of experiment, measurement, modeling, and analysis process.
Peripheral blood mononuclear cells from healthy donors and ER+ breast cancer patients were subjected to stimulation with one of 5 cytokines for 15 minutes. Cell surface markers and intracellular proteins were analyzed with spectral flow cytometry to establish cell identity and to measure response to cytokine stimulation. The response to any one of the cytokines is modeled as a sum of signal and noise. All stimulations and responses are combined to create a 6-dimensional signal response space, illustrated here in 3 dimensions. The probability of error, or signal misidentification (), and signal-to-noise ratio (SNR) are computed from the signal response space for each sample and compared across cell types, healthy donors, and breast cancer patient samples. Figure created with Biorender.