An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases
Table 2
In vitro measurements of E. coli hisRS and kcat values. The experimental measurements for three separate kinetic assays are reported for the Class II hisRS aminoacyl-tRNA synthetase enzyme. PMID denotes the PubMed ID for the reference source of the data. Abbreviations are, PP = pyrophosphate exchange, AA = aminoacylation, ST = single turnover transfer, and SC = single turnover overall chemistry. Units for kcat are in s−1 and
are in
M. nd/ns = not determined/stated.