An empirical model of aminoacylation kinetics for E. coli class I and II aminoacyl tRNA synthetases
Fig 6
Comparison between in vivo activity verses in vitro kcat measurements in class I and II aminoacyl tRNA synthetases.
Black lines with error bars indicate the range of in vivo activity for each AARS enzyme determined from proteomics data, while black triangles give the turnover rate used to calculate the number or enzymes for computational simulations. Red lines with error bars correspond to the range of experimental in vitro kcat measurements. Red triangles represent the optimized kcat values which are able to support maximum ribosome elongation rate that would be possible following a nutrient up-shift. Data for (A) class I tRNA synthetases and (B) class II tRNA synthetases.