Effects of segmentation errors on downstream-analysis in highly-multiplexed tissue imaging
Fig 5
Effect of segmentation error on Leiden clustering.
(A) Adjusted Rand Index (ARI) scores for different sizes of neighborhoods in the kNN-graph and perturbation strengths. (B) Aggregations of ARI scores for perturbation levels. (C) ARI scores for different sizes of neighborhoods in the kNN-graph and perturbation strengths for variance stabilized data. (D) ARI scores are aggregated for the number of neighbors. (E) Histogram showing the relationship between neighborhood size and resulting number of clusters. Error bars indicate one median absolute deviation. Colors encode either perturbation strength or the applied preprocessing.