Overcoming CRISPR-Cas9 off-target prediction hurdles: A novel approach with ESB rebalancing strategy and CRISPR-MCA model
Fig 3
A The new samples feature heatmaps of the severely imbalanced D5 dataset, delineating the positions and types of nucleotides substituted in the generated positive samples of gRNA-target DNA. B Validation of positive off-target sites in dataset D5 predicted by rebalanced CRISPR-MCA. The outermost layer is the real sample, the middle layer is the prediction result after using ESB rebalancing strategy, and the innermost layer is the original prediction result. Incorrect and correct predictions are indicated in red and green, respectively.