Trafficking dynamics of VEGFR1, VEGFR2, and NRP1 in human endothelial cells
Fig 2
Experimental measures of VEGFR1, VEGFR2, and NRP1: whole-cell and surface levels in HUVEC.
Western blot of total HUVEC lysates treated as indicated for 1–24 hours with 50 μg/ml cycloheximide (CHX) and stained for VEGFR1, VEGFR2 and NRP1. Representative of n = 3 replicates. B, Western blot of biotin labeling assay to measure surface and internal VEGFR1, VEGFR2, and NRP1 levels in HUVEC. Representative of n = 3 replicates. C, Western blot showing the effect of depletion by siRNA knockdown of both Rab4a and Rab11a on whole-cell VEGFR1, VEGFR2, and NRP1 levels.