The genome-scale metabolic model for the purple non-sulfur bacterium Rhodopseudomonas palustris Bis A53 accurately predicts phenotypes under chemoheterotrophic, chemoautotrophic, photoheterotrophic, and photoautotrophic growth conditions
Fig 3
Comparison of the high-throughput growth phenotypic experimental and simulated results across the three principal metabolic models.
R. palustris Bis A53 was cultivated in 190 carbon and 95 nitrogen sources under monoculture conditions for 96 hours. Subsequently, the three GEMs (iDT1294, iRpa940, and iAN1128) were properly constrained to simulate all the Biolog conditions. Each heat map compares every carbon (PM1 and PM2) and nitrogen (PM3) source’s experimental result against the simulation growth output per metabolic model. Growth results were classified into two possible results: Growth (purple) and No Growth (light pink). Additionally, we determined growth by measuring optical density (OD600, see methods) after 96 hrs, as indicated in blue to the right of each Biolog plate set. The substrates studied were sorted in descending order based on growth values. Statistical parameters to determine the global accuracy and prediction capabilities of the three models were calculated.