Effective mechanical potential of cell–cell interaction explains three-dimensional morphologies during early embryogenesis
Fig 7
Inference of effective potentials of cell–cell interaction in compaction–inhibited mouse embryos.
A. Microscopic images of embryos under chemicals. CytoD, cytochalasin D; Bleb, blebbistatin. B. Inferred DP curves in the drug–treated embryos. N = 6~10 for each condition. C. Quantitative differences in the DP curves. Distances at the potential minima in the DP curves, left panel; Distances providing 10% energy of the potential minima as the relative value to the distances at the potential minima, right panel. Mann–Whitney–Wilcoxon tests were performed and the resultant p–values for “No Drugs” vs. “EDTA”, vs. “CytoD”, and vs. “Bleb” are 0.011, 0.23, and 0.00040, respectively, in the left panel. In the right panel, distances providing other % energies instead of 10% are shown in S14B Fig. D. Confocal microscopic images of cell shapes. Cell shapes were visualized by staining F–actin or E–cadherin. E. The embryonic and cellular shapes illustrated based on D. Related figures: S11 (experimental design), S12 (enlarged view of DP curves), S13 (histological images of other embryos), and S14 (details of quantification of DP curves) Figs.