Using Fluorescence Recovery After Photobleaching data to uncover filament dynamics
Fig 5
Impact of length distributions on FRAP intensity profile curve data (type 1).
This figure compares the simulated results with random velocity and different length distributions. In panel (a) the type of length distribution changes, in panels (c)-(e) the length changes, and in (f)-(h) the mean length changes. Panel (b) is a blowup of the boxed region in panel (a). In panel (a) the average length for all distributions is 10 microns and for the Gaussian and gamma distributions the standard deviation is 0.2. In panels (c)-(e) the solid curves are plots of scaled Eq (5) such that ‖f‖∞ = 1 and the error bars are type 1 simulated results. The length of the error line is twice the standard deviation of the 50 realizations centered at the average of the realizations. The curves and error bars in (c)-(e) are profiles at times 0.5 (blue and red), 1 (light blue and orange), 3 (lighter blue and light orange), and 5 minutes (cyan and yellow). The y axis is fluorescence intensity (a.u.) in all panels. Length: (a)—varied distributions, (c)-(e)—fixed, (f)-(h)—uniform. Velocity: (a),(b), (f)-(h) gamma μ = 1, σ = 0.25; (c)-(e)—uniform on interval [0, 2].