Chaos in synthetic microbial communities
Fig 2
Overview of the pipeline for identifying chaotic topologies.
A(i) The initial model space is built from different combinations of engineering options. N1, N2, N3 are the three strains being engineered, and can optionally express QS molecules A1, A2 and bacteriocins B1, B2, B3. 4182 models are generated forming our initial model space. A(ii) We then perform ABC SMC for an oscillatory objective, which yielded 117 models that were capable of producing oscillations. A(iii) These form the prior model space for the chaos objective, using a threshold of λ1 > 0.003, we identify models capable of producing chaotic behaviour B The barchart shows the probability of models for the chaotic objective. The error bars represent the standard deviation. C An example time series representative of the chaos objective posterior distribution. Population densities as optical density (OD) show sustained, nonrepetitive oscillatory behaviour for the three species community.